#include #include #include #ifdef __GNUC__ #define LIKELY(x) __builtin_expect((x), 1) #define UNLIKELY(x) __builtin_expect((x), 0) #else #define LIKELY(x) (x) #define UNLIKELY(x) (x) #endif typedef struct { int32_t h, e; } eh_t; int ksw_normal(int qlen, const uint8_t *query, int tlen, const uint8_t *target, int m, const int8_t *mat, int o_del, int e_del, int o_ins, int e_ins, int w, int end_bonus, int zdrop, int h0, int *_qle, int *_tle, int *_gtle, int *_gscore, int *_max_off) { eh_t *eh; // score array int8_t *qp; // query profile int i, j, k, oe_del = o_del + e_del, oe_ins = o_ins + e_ins, beg, end, max, max_i, max_j, max_ins, max_del, max_ie, gscore, max_off; assert(h0 > 0); qp = malloc(qlen * m); eh = calloc(qlen + 1, 8); // generate the query profile for (k = i = 0; k < m; ++k) { const int8_t *p = &mat[k * m]; for (j = 0; j < qlen; ++j) qp[i++] = p[query[j]]; } // fill the first row eh[0].h = h0; eh[1].h = h0 > oe_ins ? h0 - oe_ins : 0; for (j = 2; j <= qlen && eh[j - 1].h > e_ins; ++j) eh[j].h = eh[j - 1].h - e_ins; // adjust $w if it is too large k = m * m; for (i = 0, max = 0; i < k; ++i) // get the max score max = max > mat[i] ? max : mat[i]; max_ins = (int)((double)(qlen * max + end_bonus - o_ins) / e_ins + 1.); max_ins = max_ins > 1 ? max_ins : 1; w = w < max_ins ? w : max_ins; max_del = (int)((double)(qlen * max + end_bonus - o_del) / e_del + 1.); max_del = max_del > 1 ? max_del : 1; w = w < max_del ? w : max_del; // TODO: is this necessary? // DP loop max = h0, max_i = max_j = -1; max_ie = -1, gscore = -1; max_off = 0; beg = 0, end = qlen; for (i = 0; LIKELY(i < tlen); ++i) { int t, f = 0, h1, m = 0, mj = -1; int8_t *q = &qp[target[i] * qlen]; // apply the band and the constraint (if provided) if (beg < i - w) beg = i - w; if (end > i + w + 1) end = i + w + 1; if (end > qlen) end = qlen; // compute the first column if (beg == 0) { h1 = h0 - (o_del + e_del * (i + 1)); if (h1 < 0) h1 = 0; } else h1 = 0; for (j = beg; LIKELY(j < end); ++j) { // At the beginning of the loop: eh[j] = { H(i-1,j-1), E(i,j) }, f = F(i,j) and h1 = H(i,j-1) // Similar to SSE2-SW, cells are computed in the following order: // H(i,j) = max{H(i-1,j-1)+S(i,j), E(i,j), F(i,j)} // E(i+1,j) = max{H(i,j)-gapo, E(i,j)} - gape // F(i,j+1) = max{H(i,j)-gapo, F(i,j)} - gape eh_t *p = &eh[j]; int h, M = p->h, e = p->e; // get H(i-1,j-1) and E(i-1,j) p->h = h1; // set H(i,j-1) for the next row M = M ? M + q[j] : 0; // separating H and M to disallow a cigar like "100M3I3D20M" h = M > e ? M : e; // e and f are guaranteed to be non-negative, so h>=0 even if M<0 h = h > f ? h : f; h1 = h; // save H(i,j) to h1 for the next column mj = m > h ? mj : j; // record the position where max score is achieved m = m > h ? m : h; // m is stored at eh[mj+1] t = M - oe_del; t = t > 0 ? t : 0; e -= e_del; e = e > t ? e : t; // computed E(i+1,j) p->e = e; // save E(i+1,j) for the next row t = M - oe_ins; t = t > 0 ? t : 0; f -= e_ins; f = f > t ? f : t; // computed F(i,j+1) } eh[end].h = h1; eh[end].e = 0; if (j == qlen) { max_ie = gscore > h1 ? max_ie : i; gscore = gscore > h1 ? gscore : h1; } if (m == 0) break; if (m > max) { max = m, max_i = i, max_j = mj; max_off = max_off > abs(mj - i) ? max_off : abs(mj - i); } else if (zdrop > 0) { if (i - max_i > mj - max_j) { if (max - m - ((i - max_i) - (mj - max_j)) * e_del > zdrop) break; } else { if (max - m - ((mj - max_j) - (i - max_i)) * e_ins > zdrop) break; } } // update beg and end for the next round for (j = beg; LIKELY(j < end) && eh[j].h == 0 && eh[j].e == 0; ++j) ; beg = j; for (j = end; LIKELY(j >= beg) && eh[j].h == 0 && eh[j].e == 0; --j) ; end = j + 2 < qlen ? j + 2 : qlen; // beg = 0; end = qlen; // uncomment this line for debugging } free(eh); free(qp); if (_qle) *_qle = max_j + 1; if (_tle) *_tle = max_i + 1; if (_gtle) *_gtle = max_ie + 1; if (_gscore) *_gscore = gscore; if (_max_off) *_max_off = max_off; return max; }