/********************************************************************************************* Description: sw align functions in bwa-mem Copyright : All right reserved by NCIC.ICT Author : Zhang Zhonghai Date : 2024/04/11 ***********************************************************************************************/ #include #include #include "utils.h" #include "align.h" kswr_t align_sse_u8(byte_mem_t *bmem, kswq_sse_t *q, int tlen, const uint8_t *target, int _o_del, int _e_del, int _o_ins, int _e_ins, int xtra) // the first gap costs -(_o+_e) { int slen, i, m_b, n_b, te = -1, gmax = 0, minsc, endsc; uint64_t *b; __m128i zero, oe_del, e_del, oe_ins, e_ins, shift, *H0, *H1, *E, *Hmax; kswr_t r; #if defined __SSE2__ #define __max_16(ret, xx) do { \ (xx) = _mm_max_epu8((xx), _mm_srli_si128((xx), 8)); \ (xx) = _mm_max_epu8((xx), _mm_srli_si128((xx), 4)); \ (xx) = _mm_max_epu8((xx), _mm_srli_si128((xx), 2)); \ (xx) = _mm_max_epu8((xx), _mm_srli_si128((xx), 1)); \ (ret) = _mm_extract_epi16((xx), 0) & 0x00ff; \ } while (0) // Given entries with arbitrary values, return whether they are all 0x00 #define allzero_16(xx) (_mm_movemask_epi8(_mm_cmpeq_epi8((xx), zero)) == 0xffff) #elif defined __ARM_NEON #define __max_16(ret, xx) (ret) = vmaxvq_u8((xx)) #define allzero_16(xx) (vmaxvq_u8((xx)) == 0) #else #define __max_16(ret, xx) (ret) = m128i_max_u8((xx)) #define allzero_16(xx) (m128i_allzero((xx))) #endif // initialization r = g_defr; minsc = (xtra&KSW_XSUBO)? xtra&0xffff : 0x10000; endsc = (xtra&KSW_XSTOP)? xtra&0xffff : 0x10000; m_b = n_b = 0; b = 0; zero = _mm_set1_epi32(0); oe_del = _mm_set1_epi8(_o_del + _e_del); e_del = _mm_set1_epi8(_e_del); oe_ins = _mm_set1_epi8(_o_ins + _e_ins); e_ins = _mm_set1_epi8(_e_ins); shift = _mm_set1_epi8(q->shift); H0 = q->H0; H1 = q->H1; E = q->E; Hmax = q->Hmax; slen = q->slen; for (i = 0; i < slen; ++i) { _mm_store_si128(E + i, zero); _mm_store_si128(H0 + i, zero); _mm_store_si128(Hmax + i, zero); } // the core loop for (i = 0; i < tlen; ++i) { int j, k, imax; __m128i e, h, t, f = zero, max = zero, *S = q->qp + target[i] * slen; // s is the 1st score vector h = _mm_load_si128(H0 + slen - 1); // h={2,5,8,11,14,17,-1,-1} in the above example h = _mm_slli_si128(h, 1); // h=H(i-1,-1); << instead of >> because x64 is little-endian for (j = 0; LIKELY(j < slen); ++j) { /* SW cells are computed in the following order: * H(i,j) = max{H(i-1,j-1)+S(i,j), E(i,j), F(i,j)} * E(i+1,j) = max{H(i,j)-q, E(i,j)-r} * F(i,j+1) = max{H(i,j)-q, F(i,j)-r} */ // compute H'(i,j); note that at the beginning, h=H'(i-1,j-1) h = _mm_adds_epu8(h, _mm_load_si128(S + j)); h = _mm_subs_epu8(h, shift); // h=H'(i-1,j-1)+S(i,j) e = _mm_load_si128(E + j); // e=E'(i,j) h = _mm_max_epu8(h, e); h = _mm_max_epu8(h, f); // h=H'(i,j) max = _mm_max_epu8(max, h); // set max _mm_store_si128(H1 + j, h); // save to H'(i,j) // now compute E'(i+1,j) e = _mm_subs_epu8(e, e_del); // e=E'(i,j) - e_del t = _mm_subs_epu8(h, oe_del); // h=H'(i,j) - o_del - e_del e = _mm_max_epu8(e, t); // e=E'(i+1,j) _mm_store_si128(E + j, e); // save to E'(i+1,j) // now compute F'(i,j+1) f = _mm_subs_epu8(f, e_ins); t = _mm_subs_epu8(h, oe_ins); // h=H'(i,j) - o_ins - e_ins f = _mm_max_epu8(f, t); // get H'(i-1,j) and prepare for the next j h = _mm_load_si128(H0 + j); // h=H'(i-1,j) } // NB: we do not need to set E(i,j) as we disallow adjecent insertion and then deletion for (k = 0; LIKELY(k < 16); ++k) { // this block mimics SWPS3; NB: H(i,j) updated in the lazy-F loop cannot exceed max f = _mm_slli_si128(f, 1); for (j = 0; LIKELY(j < slen); ++j) { h = _mm_load_si128(H1 + j); h = _mm_max_epu8(h, f); // h=H'(i,j) _mm_store_si128(H1 + j, h); h = _mm_subs_epu8(h, oe_ins); f = _mm_subs_epu8(f, e_ins); if (UNLIKELY(allzero_16(_mm_subs_epu8(f, h)))) goto end_loop16; } } end_loop16: //int k;for (k=0;k<16;++k)printf("%d ", ((uint8_t*)&max)[k]);printf("\n"); __max_16(imax, max); // imax is the maximum number in max //fprintf(stderr, "%d\n", imax); if (imax >= minsc) { // write the b array; this condition adds branching unfornately //fprintf(stderr, "%d\n", imax); if (n_b == 0 || (int32_t)b[n_b-1] + 1 != i) { // then append if (n_b == m_b) { m_b = m_b? m_b<<1 : 8; b = (uint64_t*)realloc(b, 8 * m_b); //b = (uint64_t *)byte_mem_request(bmem, 8 * m_b); } b[n_b++] = (uint64_t)imax<<32 | i; } else if ((int)(b[n_b-1]>>32) < imax) b[n_b-1] = (uint64_t)imax<<32 | i; // modify the last } if (imax > gmax) { gmax = imax; te = i; // te is the end position on the target for (j = 0; LIKELY(j < slen); ++j) // keep the H1 vector _mm_store_si128(Hmax + j, _mm_load_si128(H1 + j)); if (gmax + q->shift >= 255 || gmax >= endsc) break; } S = H1; H1 = H0; H0 = S; // swap H0 and H1 } r.score = gmax + q->shift < 255? gmax : 255; r.te = te; if (r.score != 255) { // get a->qe, the end of query match; find the 2nd best score int max = -1, tmp, low, high, qlen = slen * 16; uint8_t *t = (uint8_t*)Hmax; for (i = 0; i < qlen; ++i, ++t) if ((int)*t > max) max = *t, r.qe = i / 16 + i % 16 * slen; else if ((int)*t == max && (tmp = i / 16 + i % 16 * slen) < r.qe) r.qe = tmp; //printf("%d,%d\n", max, gmax); if (b) { i = (r.score + q->max - 1) / q->max; low = te - i; high = te + i; for (i = 0; i < n_b; ++i) { int e = (int32_t)b[i]; if ((e < low || e > high) && (int)(b[i]>>32) > r.score2) r.score2 = b[i]>>32, r.te2 = e; } } } free(b); //byte_mem_clear(bmem); return r; }