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各种统计信息的计算，metrics文件中记录的信息

  • htsjdk.samtools.metrics.StringHeader
    • 命令行参数
    • 执行命令的时间
  • METRICS CLASS picard.sam.DuplicationMetrics
LIBRARY 样本ID(建库制备的样本id)
UNPAIRED_READS_EXAMINED 未匹配的reads数量
READ_PAIRS_EXAMINED 匹配的reads数量
SECONDARY_OR_SUPPLEMENTARY_RDS 非主要匹配的reads个数
UNMAPPED_READS  未匹配的reads个数
UNPAIRED_READ_DUPLICATES    未匹配的reads，冗余的个数
READ_PAIR_DUPLICATES    匹配的reads，冗余个数
READ_PAIR_OPTICAL_DUPLICATES    匹配的reads，光学原因造成的冗余个数
PERCENT_DUPLICATION 冗余reads占比
ESTIMATED_LIBRARY_SIZE  估计的样本reads总量

normal	1205	498430	729	1205	763	117212	6877	0.235643	924111
  • HISTOGRAM java.lang.Double
BIN 坐标值
CoverageMult
all_sets
optical_sets
non_optical_sets

1.0	1.010558	287416	0	291706
2.0	1.599836	72561	6664	70093
3.0	1.943455	15542	105	14299
4.0	2.143827	3274	1	2831
5.0	2.260667	708	0	607
6.0	2.3288	136	0	114
7.0	2.368529	28	0	17
8.0	2.391696	7	0	5
9.0	2.405205	2	0	2
10.0	2.413082	0	0	0
11.0	2.417676	0	0	0
12.0	2.420354	0	0	0
13.0	2.421916	0	0	0
14.0	2.422827	0	0	0
15.0	2.423358	0	0	0
...
...
98.0	2.424101	0	0	0
99.0	2.424101	0	0	0
100.0	2.424101	0	0	0