/* Description: read ends结构体主要用来标记冗余,包含一些序列的测序过程中的物理信息等 Copyright : All right reserved by ICT Author : Zhang Zhonghai Date : 2023/11/3 */ #ifndef READ_ENDS_H_ #define READ_ENDS_H_ #include /* 包含了所有read ends信息,如picard里边的 ReadEndsForMarkDuplicates*/ struct ReadEnds { /* PhysicalLocationInt中的成员变量 */ /** * Small class that provides access to the physical location information about a cluster. * All values should be defaulted to -1 if unavailable. Tile should only allow * non-zero positive integers, x and y coordinates must be non-negative. * This is different from PhysicalLocationShort in that the x and y positions are ints, not shorts * thus, they do not overflow within a HiSeqX tile. */ int16_t tile = -1; int32_t x = -1; int32_t y = -1; /* ReadEnds中的成员变量 */ /** Little struct-like class to hold read pair (and fragment) end data for duplicate marking. */ static const int8_t F = 0, R = 1, FF = 2, FR = 3, RR = 4, RF = 5; int16_t libraryId; int8_t orientation; int32_t read1ReferenceIndex = -1; int32_t read1Coordinate = -1; int32_t read2ReferenceIndex = -1; int32_t read2Coordinate = -1; // This field is overloaded for flow based processing as the end coordinate of read 1. (paired reads not supported) /* Additional information used to detect optical dupes */ int16_t readGroup = -1; /** For optical duplicate detection the orientation matters regard to 1st or 2nd end of a mate */ int8_t orientationForOpticalDuplicates = -1; /** A *transient* flag marking this read end as being an optical duplicate. */ bool isOpticalDuplicate = false; /* ReadEndsForMarkDuplicates中的成员变量 */ /** Little struct-like class to hold read pair (and fragment) end data for MarkDuplicatesWithMateCigar **/ int16_t score = 0; int64_t read1IndexInFile = -1; int64_t read2IndexInFile = -1; int64_t duplicateSetSize = -1; /* ReadEndsForMarkDuplicatesWithBarcodes中的成员变量 (好像用不到) */ int32_t barcode = 0; // primary barcode for this read (and pair) int32_t readOneBarcode = 0; // read one barcode, 0 if not present int32_t readTwoBarcode = 0; // read two barcode, 0 if not present or not paired }; #endif