#!/usr/bin/env k8 var getopt = function(args, ostr) { var oli; // option letter list index if (typeof(getopt.place) == 'undefined') getopt.ind = 0, getopt.arg = null, getopt.place = -1; if (getopt.place == -1) { // update scanning pointer if (getopt.ind >= args.length || args[getopt.ind].charAt(getopt.place = 0) != '-') { getopt.place = -1; return null; } if (getopt.place + 1 < args[getopt.ind].length && args[getopt.ind].charAt(++getopt.place) == '-') { // found "--" ++getopt.ind; getopt.place = -1; return null; } } var optopt = args[getopt.ind].charAt(getopt.place++); // character checked for validity if (optopt == ':' || (oli = ostr.indexOf(optopt)) < 0) { if (optopt == '-') return null; // if the user didn't specify '-' as an option, assume it means null. if (getopt.place < 0) ++getopt.ind; return '?'; } if (oli+1 >= ostr.length || ostr.charAt(++oli) != ':') { // don't need argument getopt.arg = null; if (getopt.place < 0 || getopt.place >= args[getopt.ind].length) ++getopt.ind, getopt.place = -1; } else { // need an argument if (getopt.place >= 0 && getopt.place < args[getopt.ind].length) getopt.arg = args[getopt.ind].substr(getopt.place); else if (args.length <= ++getopt.ind) { // no arg getopt.place = -1; if (ostr.length > 0 && ostr.charAt(0) == ':') return ':'; return '?'; } else getopt.arg = args[getopt.ind]; // white space getopt.place = -1; ++getopt.ind; } return optopt; } var colors = ["0,128,255", "255,0,0", "0,192,0"]; function print_lines(a, fmt) { if (a.length == 0) return; if (fmt == "bed") { var n_pri = 0; for (var i = 0; i < a.length; ++i) if (a[i][8] == 0) ++n_pri; if (n_pri > 1) { for (var i = 0; i < a.length; ++i) if (a[i][8] == 0) a[i][8] = 1; } else if (n_pri == 0) { warn("Warning: " + a[0][3] + " doesn't have a primary alignment"); } for (var i = 0; i < a.length; ++i) { a[i][8] = colors[a[i][8]]; print(a[i].join("\t")); } } a.length = 0; } function main(args) { var re = /(\d+)([MIDNSH])/g; var c, fmt = "bed", fn_name_conv = null; while ((c = getopt(args, "f:n:")) != null) { if (c == 'f') fmt = getopt.arg; else if (c == 'n') fn_name_conv = getopt.arg; } if (getopt.ind == args.length) { warn("Usage: k8 splice2bed.js |"); exit(1); } var conv = null; if (fn_name_conv != null) { conv = new Map(); var file = new File(fn_name_conv); var buf = new Bytes(); while (file.readline(buf) >= 0) { var t = buf.toString().split("\t"); conv.put(t[0], t[1]); } buf.destroy(); file.close(); } var file = args[getopt.ind] == '-'? new File() : new File(args[getopt.ind]); var buf = new Bytes(); var a = []; while (file.readline(buf) >= 0) { var line = buf.toString(); if (line.charAt(0) == '@') continue; // skip SAM header lines var t = line.split("\t"); var is_pri = false, cigar = null, a1; var qname = conv != null? conv.get(t[0]) : null; if (qname != null) t[0] = qname; if (t.length >= 10 && t[4] != '+' && t[4] != '-' && /^\d+/.test(t[1])) { // SAM var flag = parseInt(t[1]); if (flag&1) t[0] += '/' + (flag>>6&3); } if (a.length && a[0][3] != t[0]) { print_lines(a, fmt); a = []; } if (t.length >= 12 && (t[4] == '+' || t[4] == '-')) { // PAF for (var i = 12; i < t.length; ++i) { if (t[i].substr(0, 5) == 'cg:Z:') { cigar = t[i].substr(5); } else if (t[i].substr(0, 5) == 's2:i:') { is_pri = true; } } a1 = [t[5], t[7], t[8], t[0], Math.floor(t[9]/t[10]*1000), t[4]]; } else if (t.length >= 10) { // SAM var flag = parseInt(t[1]); if ((flag&4) || a[2] == '*') continue; cigar = t[5]; is_pri = (flag&0x100)? false : true; a1 = [t[2], parseInt(t[3])-1, null, t[0], 1000, (flag&16)? '-' : '+']; } else { throw Error("unrecognized input format"); } if (cigar == null) throw Error("missing CIGAR"); var m, x0 = 0, x = 0, bs = [], bl = []; while ((m = re.exec(cigar)) != null) { if (m[2] == 'M' || m[2] == 'D') { x += parseInt(m[1]); } else if (m[2] == 'N') { bs.push(x0); bl.push(x - x0); x += parseInt(m[1]); x0 = x; } } bs.push(x0); bl.push(x - x0); // write the BED12 line if (a1[2] == null) a1[2] = a1[1] + x; a1.push(a1[1], a1[2]); // thick start/end is the same as start/end a1.push(is_pri? 0 : 2, bs.length, bl.join(",")+",", bs.join(",")+","); a.push(a1); } print_lines(a, fmt); buf.destroy(); file.close(); if (conv != null) conv.destroy(); } main(arguments);