#include #include #include "minimap.h" #include "ksw2.h" #ifndef kroundup32 #define kroundup32(x) (--(x), (x)|=(x)>>1, (x)|=(x)>>2, (x)|=(x)>>4, (x)|=(x)>>8, (x)|=(x)>>16, ++(x)) #endif static void ksw_gen_simple_mat(int m, int8_t *mat, int8_t a, int8_t b) { int i, j; a = a < 0? -a : a; b = b > 0? -b : b; for (i = 0; i < m - 1; ++i) { for (j = 0; j < m - 1; ++j) mat[i * m + j] = i == j? a : b; mat[i * m + m - 1] = 0; } for (j = 0; j < m; ++j) mat[(m - 1) * m + j] = 0; } static inline void mm_seq_rev(uint32_t len, uint8_t *seq) { uint32_t i; uint8_t t; for (i = 0; i < len>>1; ++i) t = seq[i], seq[i] = seq[len - 1 - i], seq[len - 1 - i] = t; } static void mm_append_cigar(mm_reg1_t *r, uint32_t n_cigar, uint32_t *cigar) // TODO: this calls the libc realloc() { if (n_cigar == 0) return; if (r->cigar == 0) { uint32_t m_cigar = n_cigar + 2; kroundup32(m_cigar); r->cigar = (mm_cigar_t*)malloc(m_cigar * 4); r->cigar->n = 0, r->cigar->m = m_cigar; } else if (r->cigar->n + n_cigar > r->cigar->m - 2) { r->cigar->m = r->cigar->n + n_cigar + 2; kroundup32(r->cigar->m); r->cigar = (mm_cigar_t*)realloc(r->cigar, r->cigar->m * 4); } if (r->cigar->n > 0 && (r->cigar->cigar[r->cigar->n-1]&0xf) == (cigar[0]&0xf)) { // same CIGAR op at the boundary r->cigar->cigar[r->cigar->n-1] += cigar[0]>>4<<4; if (n_cigar > 1) memcpy(r->cigar->cigar + r->cigar->n, cigar + 1, (n_cigar - 1) * 4); r->cigar->n += n_cigar - 1; } else { memcpy(r->cigar->cigar + r->cigar->n, cigar, n_cigar * 4); r->cigar->n += n_cigar; } } static void mm_align1(void *km, const mm_mapopt_t *opt, const mm_idx_t *mi, int qlen, uint8_t *qseq0[2], mm_reg1_t *r, mm_reg1_t *r2, mm128_t *a, ksw_extz_t *ez) { int32_t rid = a[r->as].x<<1>>33, rev = a[r->as].x>>63; uint8_t *tseq, *qseq; int32_t i, k, l, rs0, re0, qs0, qe0; int32_t rs, re, qs, qe; int32_t rs1, qs1; int8_t mat[25]; ksw_gen_simple_mat(5, mat, opt->a, opt->b); rs = (int32_t)a[r->as].x + 1; // NB: this is the same as r->{rs,re} re = (int32_t)a[r->as + r->cnt - 1].x + 1; qs = (int32_t)a[r->as].y + 1; // NB: this is the coordinate on the reverse strand; r->{qs,qe} are on the reverse strand qe = (int32_t)a[r->as + r->cnt - 1].y + 1; if (qs > 0 && rs > 0) { l = qs < opt->max_gap? qs : opt->max_gap; qs0 = qs - l; l = (l * opt->a - opt->q) / opt->e; l = l < opt->max_gap? l : opt->max_gap; l = l < rs? l : rs; rs0 = rs - l; } else rs0 = rs, qs0 = qs; if (qe < qlen && re < mi->seq[rid].len) { l = qlen - re < opt->max_gap? qlen - re : opt->max_gap; qe0 = qe + l; l = (l * opt->a - opt->q) / opt->e; l = l < opt->max_gap? l : opt->max_gap; l = l < mi->seq[rid].len - re? l : mi->seq[rid].len - re; re0 = re + l; } else re0 = re, qe0 = qe; tseq = (uint8_t*)kmalloc(km, re0 - rs0); if (qs > 0 && rs > 0) { // left extension uint32_t ql = qs - qs0, tl = rs - rs0; qseq = &qseq0[rev][qs0]; mm_idx_getseq(mi, rid, rs0, rs, tseq); mm_seq_rev(ql, qseq); mm_seq_rev(tl, tseq); #if 0 fprintf(stderr, "===> [-1] %d-%d %c (%s:%d-%d) <===\n", qs0, qs, "+-"[rev], mi->seq[rid].name, rs0, rs); for (k = 0; k < tl; ++k) fputc("ACGTN"[tseq[k]], stderr); fputc('\n', stderr); for (k = 0; k < ql; ++k) fputc("ACGTN"[qseq[k]], stderr); fputc('\n', stderr); #endif ksw_extz2_sse(km, ql, qseq, tl, tseq, 5, mat, opt->q, opt->e, (int)(opt->bw * 1.5 + .499), opt->zdrop, KSW_EZ_EXTZ_ONLY|KSW_EZ_RIGHT|KSW_EZ_REV_CIGAR, ez); mm_seq_rev(ql, qseq); mm_append_cigar(r, ez->n_cigar, ez->cigar); rs1 = rs - (ez->max_t + 1); qs1 = qs - (ez->max_q + 1); } else rs1 = rs, qs1 = qs; fprintf(stderr, "%d,%d\n", rs1, qs1); for (i = 0; i < r->cigar->n; ++i) fprintf(stderr, "%d%c", r->cigar->cigar[i]>>4, "MID"[r->cigar->cigar[i]&0xf]); fputc('\n', stderr); for (i = 1; i < r->cnt; ++i) { // gap filling re = (int32_t)a[r->as + i].x + 1; qe = (int32_t)a[r->as + i].y + 1; if (i == r->cnt - 1 || qe - qs >= opt->min_ksw_len || re - rs >= opt->min_ksw_len) { qseq = &qseq0[rev][qs]; mm_idx_getseq(mi, rid, rs, re, tseq); #if 1 fprintf(stderr, "===> [%d] %d-%d %c (%s:%d-%d) <===\n", i, qs, qe, "+-"[rev], mi->seq[rid].name, rs, re); for (k = 0; k < re - rs; ++k) fputc("ACGTN"[tseq[k]], stderr); fputc('\n', stderr); for (k = 0; k < qe - qs; ++k) fputc("ACGTN"[qseq[k]], stderr); fputc('\n', stderr); #endif ksw_extz2_sse(km, qe-qs, qseq, re-rs, tseq, 5, mat, opt->q, opt->e, (int)(opt->bw * 1.5 + .499), opt->zdrop, KSW_EZ_DYN_BAND, ez); if (ez->score == KSW_NEG_INF) { // truncated by Z-drop } else { mm_append_cigar(r, ez->n_cigar, ez->cigar); } for (i = 0; i < r->cigar->n; ++i) fprintf(stderr, "%d%c", r->cigar->cigar[i]>>4, "MID"[r->cigar->cigar[i]&0xf]); fputc('\n', stderr); rs = re, qs = qe; } } if (i == r->cnt) { } // fprintf(stderr, "%d,%d\n", rs1, qs1); for (i = 0; i < r->cigar->n; ++i) fprintf(stderr, "%d%c", r->cigar->cigar[i]>>4, "MID"[r->cigar->cigar[i]&0xf]); fputc('\n', stderr); kfree(km, tseq); } void mm_align_skeleton(void *km, const mm_mapopt_t *opt, const mm_idx_t *mi, int qlen, const char *qstr, int n_regs, mm_reg1_t *regs, mm128_t *a) { extern unsigned char seq_nt4_table[256]; int i, reg; uint8_t *qseq0[2]; ksw_extz_t ez; memset(&ez, 0, sizeof(ksw_extz_t)); qseq0[0] = (uint8_t*)kmalloc(km, qlen); qseq0[1] = (uint8_t*)kmalloc(km, qlen); for (i = 0; i < qlen; ++i) { qseq0[0][i] = seq_nt4_table[(uint8_t)qstr[i]]; qseq0[1][qlen - 1 - i] = qseq0[0][i] < 4? 3 - qseq0[0][i] : 4; } for (reg = 0; reg < n_regs; ++reg) { mm_reg1_t r2; mm_align1(km, opt, mi, qlen, qseq0, ®s[reg], &r2, a, &ez); } kfree(km, qseq0[0]); kfree(km, qseq0[1]); kfree(km, ez.cigar); }