#include #include "minimap.h" #include "ksw2.h" static void mm_align1(void *km, const mm_mapopt_t *opt, const mm_idx_t *mi, int qlen, uint8_t *qseq0[2], mm_reg1_t *r, mm_reg1_t *r_split, mm128_t *a) { int32_t rid = a[r->as].x<<1>>33, rev = a[r->as].x>>63; uint8_t *tseq0, *tseq, *qseq; int32_t i, l, rs0, re0; int32_t rs, re, qs, qe, ret; l = r->qs < opt->max_gap? r->qs : opt->max_gap; l = (l * opt->a - opt->q) / opt->e; l = l < opt->max_gap? l : opt->max_gap; l = l < r->rs? l : r->rs; rs0 = r->rs - l; l = qlen - r->re < opt->max_gap? qlen - r->re : opt->max_gap; l = (l * opt->a - opt->q) / opt->e; l = l < opt->max_gap? l : opt->max_gap; l = l < mi->seq[rid].len - r->re? l : mi->seq[rid].len - r->re; re0 = r->re + l; tseq0 = (uint8_t*)kmalloc(km, re0 - rs0); ret = mm_idx_getseq(mi, rid, rs0, re0, tseq0); assert(ret > 0); rs = (int32_t)a[r->as].x + 1; qs = (int32_t)a[r->as].y + 1; for (i = 1; i < r->cnt; ++i) { re = (int32_t)a[r->as + i].x + 1; qe = (int32_t)a[r->as + i].y + 1; if (i == r->cnt - 1 || qe - qs >= opt->min_ksw_len || re - rs >= opt->min_ksw_len) { qseq = &qseq0[rev][qs]; tseq = &tseq0[rs - rs0]; #if 0 fprintf(stderr, "===> [%d] %d-%d %c (%s:%d-%d) <===\n", i, qs, qe, "+-"[rev], mi->seq[rid].name, rs, re); for (k = 0; k < re - rs; ++k) fputc("ACGTN"[tseq[k]], stderr); fputc('\n', stderr); for (k = 0; k < qe - qs; ++k) fputc("ACGTN"[qseq[k]], stderr); fputc('\n', stderr); #endif rs = re, qs = qe; } } kfree(km, tseq0); } void mm_align_skeleton(void *km, const mm_mapopt_t *opt, const mm_idx_t *mi, int qlen, const char *qstr, int n_regs, mm_reg1_t *regs, mm128_t *a) { extern unsigned char seq_nt4_table[256]; int i, reg; uint8_t *qseq0[2]; qseq0[0] = (uint8_t*)kmalloc(km, qlen); qseq0[1] = (uint8_t*)kmalloc(km, qlen); for (i = 0; i < qlen; ++i) { qseq0[0][i] = seq_nt4_table[(uint8_t)qstr[i]]; qseq0[1][qlen - 1 - i] = qseq0[0][i] < 4? 3 - qseq0[0][i] : 4; } for (reg = 0; reg < n_regs; ++reg) { mm_reg1_t r_split; mm_align1(km, opt, mi, qlen, qseq0, ®s[reg], &r_split, a); } kfree(km, qseq0[0]); kfree(km, qseq0[1]); }