#include #include #include #include #include #include "kalloc.h" #include "mmpriv.h" static char mm_rg_id[256]; static inline void str_enlarge(kstring_t *s, int l) { if (s->l + l + 1 > s->m) { s->m = s->l + l + 1; kroundup32(s->m); s->s = (char*)realloc(s->s, s->m); } } static inline void str_copy(kstring_t *s, const char *st, const char *en) { str_enlarge(s, en - st); memcpy(&s->s[s->l], st, en - st); s->l += en - st; } static void mm_sprintf_lite(kstring_t *s, const char *fmt, ...) { char buf[16]; // for integer to string conversion const char *p, *q; va_list ap; va_start(ap, fmt); for (q = p = fmt; *p; ++p) { if (*p == '%') { if (p > q) str_copy(s, q, p); ++p; if (*p == 'd') { int c, i, l = 0; unsigned int x; c = va_arg(ap, int); x = c >= 0? c : -c; do { buf[l++] = x%10 + '0'; x /= 10; } while (x > 0); if (c < 0) buf[l++] = '-'; str_enlarge(s, l); for (i = l - 1; i >= 0; --i) s->s[s->l++] = buf[i]; } else if (*p == 's') { char *r = va_arg(ap, char*); str_copy(s, r, r + strlen(r)); } else if (*p == 'c') { str_enlarge(s, 1); s->s[s->l++] = va_arg(ap, int); } else abort(); q = p + 1; } } if (p > q) str_copy(s, q, p); va_end(ap); s->s[s->l] = 0; } static char *mm_escape(char *s) { char *p, *q; for (p = q = s; *p; ++p) { if (*p == '\\') { ++p; if (*p == 't') *q++ = '\t'; else if (*p == '\\') *q++ = '\\'; } else *q++ = *p; } *q = '\0'; return s; } static void sam_write_rg_line(kstring_t *str, const char *s) { char *p, *q, *r, *rg_line = 0; memset(mm_rg_id, 0, 256); if (s == 0) return; if (strstr(s, "@RG") != s) { if (mm_verbose >= 1) fprintf(stderr, "[ERROR] the read group line is not started with @RG\n"); goto err_set_rg; } if (strstr(s, "\t") != NULL) { if (mm_verbose >= 1) fprintf(stderr, "[ERROR] the read group line contained literal characters -- replace with escaped tabs: \\t\n"); goto err_set_rg; } rg_line = strdup(s); mm_escape(rg_line); if ((p = strstr(rg_line, "\tID:")) == 0) { if (mm_verbose >= 1) fprintf(stderr, "[ERROR] no ID within the read group line\n"); goto err_set_rg; } p += 4; for (q = p; *q && *q != '\t' && *q != '\n'; ++q); if (q - p + 1 > 256) { if (mm_verbose >= 1) fprintf(stderr, "[ERROR] @RG:ID is longer than 255 characters\n"); goto err_set_rg; } for (q = p, r = mm_rg_id; *q && *q != '\t' && *q != '\n'; ++q) *r++ = *q; mm_sprintf_lite(str, "%s\n", rg_line); err_set_rg: free(rg_line); } void mm_write_sam_hdr(const mm_idx_t *idx, const char *rg, const char *ver, int argc, char *argv[]) { kstring_t str = {0,0,0}; if (idx) { uint32_t i; for (i = 0; i < idx->n_seq; ++i) printf("@SQ\tSN:%s\tLN:%d\n", idx->seq[i].name, idx->seq[i].len); } if (rg) sam_write_rg_line(&str, rg); mm_sprintf_lite(&str, "@PG\tID:minimap2\tPN:minimap2"); if (ver) mm_sprintf_lite(&str, "\tVN:%s", ver); if (argc > 1) { int i; mm_sprintf_lite(&str, "\tCL:minimap2"); for (i = 1; i < argc; ++i) mm_sprintf_lite(&str, " %s", argv[i]); } mm_sprintf_lite(&str, "\n"); fputs(str.s, stdout); free(str.s); } static void write_cs(void *km, kstring_t *s, const mm_idx_t *mi, const mm_bseq1_t *t, const mm_reg1_t *r, int no_iden) { extern unsigned char seq_nt4_table[256]; int i, q_off, t_off; uint8_t *qseq, *tseq; char *tmp; if (r->p == 0) return; mm_sprintf_lite(s, "\tcs:Z:"); qseq = (uint8_t*)kmalloc(km, r->qe - r->qs); tseq = (uint8_t*)kmalloc(km, r->re - r->rs); tmp = (char*)kmalloc(km, r->re - r->rs > r->qe - r->qs? r->re - r->rs + 1 : r->qe - r->qs + 1); mm_idx_getseq(mi, r->rid, r->rs, r->re, tseq); if (!r->rev) { for (i = r->qs; i < r->qe; ++i) qseq[i - r->qs] = seq_nt4_table[(uint8_t)t->seq[i]]; } else { for (i = r->qs; i < r->qe; ++i) { uint8_t c = seq_nt4_table[(uint8_t)t->seq[i]]; qseq[r->qe - i - 1] = c >= 4? 4 : 3 - c; } } for (i = q_off = t_off = 0; i < r->p->n_cigar; ++i) { int j, op = r->p->cigar[i]&0xf, len = r->p->cigar[i]>>4; assert(op >= 0 && op <= 3); if (op == 0) { int l_tmp = 0; for (j = 0; j < len; ++j) { if (qseq[q_off + j] != tseq[t_off + j]) { if (l_tmp > 0) { if (!no_iden) { tmp[l_tmp] = 0; mm_sprintf_lite(s, "=%s", tmp); } else mm_sprintf_lite(s, ":%d", l_tmp); l_tmp = 0; } mm_sprintf_lite(s, "*%c%c", "acgtn"[tseq[t_off + j]], "acgtn"[qseq[q_off + j]]); } else tmp[l_tmp++] = "ACGTN"[qseq[q_off + j]]; } if (l_tmp > 0) { if (!no_iden) { tmp[l_tmp] = 0; mm_sprintf_lite(s, "=%s", tmp); } else mm_sprintf_lite(s, ":%d", l_tmp); } q_off += len, t_off += len; } else if (op == 1) { for (j = 0, tmp[len] = 0; j < len; ++j) tmp[j] = "acgtn"[qseq[q_off + j]]; mm_sprintf_lite(s, "+%s", tmp); q_off += len; } else if (op == 2 || (op == 3 && len < 4)) { for (j = 0, tmp[len] = 0; j < len; ++j) tmp[j] = "acgtn"[tseq[t_off + j]]; mm_sprintf_lite(s, "-%s", tmp); t_off += len; } else { // op == 3 && len >= 4 mm_sprintf_lite(s, "~%c%c%d%c%c", "acgtn"[tseq[t_off]], "acgtn"[tseq[t_off+1]], len, "acgtn"[tseq[t_off+len-2]], "acgtn"[tseq[t_off+len-1]]); t_off += len; } } assert(t_off == r->re - r->rs && q_off == r->qe - r->qs); kfree(km, qseq); kfree(km, tseq); kfree(km, tmp); } static inline void write_tags(kstring_t *s, const mm_reg1_t *r) { int type = r->inv? 'I' : r->id == r->parent? 'P' : 'S'; mm_sprintf_lite(s, "\ttp:A:%c\tcm:i:%d\ts1:i:%d", type, r->cnt, r->score); if (r->parent == r->id) mm_sprintf_lite(s, "\ts2:i:%d", r->subsc); if (r->split) mm_sprintf_lite(s, "\tzd:i:%d", r->split); if (r->p) { mm_sprintf_lite(s, "\tNM:i:%d\tms:i:%d\tAS:i:%d\tnn:i:%d", r->p->n_diff, r->p->dp_max, r->p->dp_score, r->p->n_ambi); if (r->p->trans_strand == 1 || r->p->trans_strand == 2) mm_sprintf_lite(s, "\tts:A:%c", "?+-?"[r->p->trans_strand]); } } void mm_write_paf(kstring_t *s, const mm_idx_t *mi, const mm_bseq1_t *t, const mm_reg1_t *r, void *km, int opt_flag) { s->l = 0; mm_sprintf_lite(s, "%s\t%d\t%d\t%d\t%c\t", t->name, t->l_seq, r->qs, r->qe, "+-"[r->rev]); if (mi->seq[r->rid].name) mm_sprintf_lite(s, "%s", mi->seq[r->rid].name); else mm_sprintf_lite(s, "%d", r->rid); mm_sprintf_lite(s, "\t%d\t%d\t%d", mi->seq[r->rid].len, r->rs, r->re); if (r->p) mm_sprintf_lite(s, "\t%d\t%d", r->p->blen - r->p->n_ambi - r->p->n_diff, r->p->blen); else mm_sprintf_lite(s, "\t%d\t%d", r->fuzzy_mlen, r->fuzzy_blen); mm_sprintf_lite(s, "\t%d", r->mapq); write_tags(s, r); if (r->p && (opt_flag & MM_F_OUT_CG)) { uint32_t k; mm_sprintf_lite(s, "\tcg:Z:"); for (k = 0; k < r->p->n_cigar; ++k) mm_sprintf_lite(s, "%d%c", r->p->cigar[k]>>4, "MIDN"[r->p->cigar[k]&0xf]); } if (r->p && (opt_flag & MM_F_OUT_CS)) write_cs(km, s, mi, t, r, opt_flag&MM_F_CS_NO_EQUAL); } static void sam_write_sq(kstring_t *s, char *seq, int l, int rev, int comp) { extern unsigned char seq_comp_table[256]; if (rev) { int i; str_enlarge(s, l); for (i = 0; i < l; ++i) { int c = seq[l - 1 - i]; s->s[s->l + i] = c < 128 && comp? seq_comp_table[c] : c; } s->l += l; } else str_copy(s, seq, seq + l); } void mm_write_sam2(kstring_t *s, const mm_idx_t *mi, const mm_bseq1_t *t, int seg_idx, int reg_idx, int n_seg, const int *n_regss, const mm_reg1_t *const* regss, void *km) { int flag = n_seg > 1? 0x1 : 0x0, n_regs = n_regss[seg_idx]; const mm_reg1_t *regs = regss[seg_idx]; const mm_reg1_t *r = n_regs > 0 && reg_idx < n_regs && reg_idx >= 0? ®s[reg_idx] : NULL; // write QNAME s->l = 0; mm_sprintf_lite(s, "%s", t->name); if (n_seg > 1) s->l = mm_qname_len(t->name); // trim the suffix like /1 or /2 // write up to CIGAR if (r == 0) { mm_sprintf_lite(s, "\t4\t*\t0\t0\t*\t"); } else { if (r->rev) flag |= 0x10; if (r->parent != r->id) flag |= 0x100; else if (!r->sam_pri) flag |= 0x800; if (n_seg > 1) { if (seg_idx == 0) flag |= 0x40; else if (seg_idx == n_seg - 1) flag |= 0x80; // TODO: set 0x2!!! } mm_sprintf_lite(s, "\t%d\t%s\t%d\t%d\t", flag, mi->seq[r->rid].name, r->rs+1, r->mapq); if (r->p) { // actually this should always be true for SAM output uint32_t k, clip_len = r->rev? t->l_seq - r->qe : r->qs; int clip_char = (flag&0x800)? 'H' : 'S'; if (clip_len) mm_sprintf_lite(s, "%d%c", clip_len, clip_char); for (k = 0; k < r->p->n_cigar; ++k) mm_sprintf_lite(s, "%d%c", r->p->cigar[k]>>4, "MIDN"[r->p->cigar[k]&0xf]); clip_len = r->rev? r->qs : t->l_seq - r->qe; if (clip_len) mm_sprintf_lite(s, "%d%c", clip_len, clip_char); } else mm_sprintf_lite(s, "*"); } // write mate positions if (n_seg > 1) { mm_sprintf_lite(s, "\t*\t0\t0\t"); } else mm_sprintf_lite(s, "\t*\t0\t0\t"); // write SEQ and QUAL if (r == 0) { sam_write_sq(s, t->seq, t->l_seq, 0, 0); mm_sprintf_lite(s, "\t"); if (t->qual) sam_write_sq(s, t->qual, t->l_seq, 0, 0); else mm_sprintf_lite(s, "*"); } else { if ((flag & 0x900) == 0) { sam_write_sq(s, t->seq, t->l_seq, r->rev, r->rev); mm_sprintf_lite(s, "\t"); if (t->qual) sam_write_sq(s, t->qual, t->l_seq, r->rev, 0); else mm_sprintf_lite(s, "*"); } else if (flag & 0x100) { mm_sprintf_lite(s, "*\t*"); } else { sam_write_sq(s, t->seq + r->qs, r->qe - r->qs, r->rev, r->rev); mm_sprintf_lite(s, "\t"); if (t->qual) sam_write_sq(s, t->qual + r->qs, r->qe - r->qs, r->rev, 0); else mm_sprintf_lite(s, "*"); } } // write tags if (mm_rg_id[0]) mm_sprintf_lite(s, "\tRG:Z:%s", mm_rg_id); if (n_seg > 2) mm_sprintf_lite(s, "\tFI:i:%d", seg_idx); if (r) { write_tags(s, r); if (r->parent == r->id && r->p && n_regs > 1 && regs && r >= regs && r - regs < n_regs) { // supplementary aln may exist int i, n_sa = 0; // n_sa: number of SA fields for (i = 0; i < n_regs; ++i) if (i != r - regs && regs[i].parent == regs[i].id && regs[i].p) ++n_sa; if (n_sa > 0) { mm_sprintf_lite(s, "\tSA:Z:"); for (i = 0; i < n_regs; ++i) { const mm_reg1_t *q = ®s[i]; int l_M, l_I = 0, l_D = 0, clip5 = 0, clip3 = 0; if (r == q || q->parent != q->id || q->p == 0) continue; if (q->qe - q->qs < q->re - q->rs) l_M = q->qe - q->qs, l_D = (q->re - q->rs) - l_M; else l_M = q->re - q->rs, l_I = (q->qe - q->qs) - l_M; clip5 = q->rev? t->l_seq - q->qe : q->qs; clip3 = q->rev? q->qs : t->l_seq - q->qe; mm_sprintf_lite(s, "%s,%d,%c,", mi->seq[q->rid].name, q->rs+1, "+-"[q->rev]); if (clip5) mm_sprintf_lite(s, "%dS", clip5); if (l_M) mm_sprintf_lite(s, "%dM", l_M); if (l_I) mm_sprintf_lite(s, "%dI", l_I); if (l_D) mm_sprintf_lite(s, "%dD", l_D); if (clip3) mm_sprintf_lite(s, "%dS", clip3); mm_sprintf_lite(s, ",%d,%d;", q->mapq, q->p->n_diff); } } } } s->s[s->l] = 0; // we always have room for an extra byte (see str_enlarge) } void mm_write_sam(kstring_t *s, const mm_idx_t *mi, const mm_bseq1_t *t, const mm_reg1_t *r, int n_regs, const mm_reg1_t *regs) { int i; for (i = 0; i < n_regs; ++i) if (r == ®s[i]) break; mm_write_sam2(s, mi, t, 0, i, 1, &n_regs, ®s, NULL); }