#include #include #include "mmpriv.h" #include "kalloc.h" mm_reg1_t *mm_gen_regs(void *km, int qlen, int n_u, uint64_t *u, mm128_t *a) // convert chains to hits { mm128_t *z, tmp; mm_reg1_t *r; int i, k; if (n_u == 0) return 0; // sort by score z = (mm128_t*)kmalloc(km, n_u * 16); for (i = k = 0; i < n_u; ++i) { z[i].x = u[i] >> 32; z[i].y = (uint64_t)k << 32 | (int32_t)u[i]; k += (int32_t)u[i]; } radix_sort_128x(z, z + n_u); for (i = 0; i < n_u>>1; ++i) // reverse, s.t. larger score first tmp = z[i], z[i] = z[n_u-1-i], z[n_u-1-i] = tmp; // populate r[] r = (mm_reg1_t*)calloc(n_u, sizeof(mm_reg1_t)); for (i = 0; i < n_u; ++i) { mm_reg1_t *ri = &r[i]; ri->id = i; ri->parent = MM_PARENT_UNSET; ri->score = z[i].x; ri->cnt = (int32_t)z[i].y; ri->as = z[i].y >> 32; mm_reg_set_coor(ri, qlen, a); } kfree(km, z); return r; } void mm_split_reg(mm_reg1_t *r, mm_reg1_t *r2, int n, int qlen, mm128_t *a) { if (n <= 0 || n >= r->cnt) return; *r2 = *r; r2->id = -1; r2->sam_pri = 0; r2->p = 0; r2->cnt = r->cnt - n; r2->score = (int32_t)(r->score * ((float)r2->cnt / r->cnt) + .499); r2->as = r->as + n; if (r->parent == r->id) r2->parent = MM_PARENT_TMP_PRI; mm_reg_set_coor(r2, qlen, a); r->cnt -= r2->cnt; r->score -= r2->score; mm_reg_set_coor(r, qlen, a); r->split |= 1, r2->split |= 2; } void mm_set_parent(void *km, float mask_level, int n, mm_reg1_t *r) // and compute mm_reg1_t::subsc { int i, j, k, *w; if (n <= 0) return; w = (int*)kmalloc(km, n * sizeof(int)); w[0] = 0, r[0].parent = 0; for (i = 1, k = 1; i < n; ++i) { int si = r[i].qs, ei = r[i].qe; for (j = 0; j < k; ++j) { int sj = r[w[j]].qs, ej = r[w[j]].qe; int min = ej - sj < ei - si? ej - sj : ei - si; int ol = si < sj? (ei < sj? 0 : ei < ej? ei - sj : ej - sj) : (ej < si? 0 : ej < ei? ej - si : ei - si); if (ol > mask_level * min) { r[i].parent = r[w[j]].parent; if (r[w[j]].subsc < r[i].score) r[w[j]].subsc = r[i].score; break; } } if (j == k) w[k++] = i, r[i].parent = i; } kfree(km, w); } void mm_update_parent(void *km, float mask_level, int n, mm_reg1_t *r) // due to changes to r.{qs,qe} after DP extension { int i, j, k, *w, n_pri = 0; if (n <= 0) return; for (i = 0; i < n; ++i) if (r[i].id == r[i].parent) ++n_pri; if (n_pri <= 1) return; w = (int*)kmalloc(km, n_pri * sizeof(int)); for (i = j = 0; i < n; ++i) // find the first primary if (r[i].id == r[i].parent) break; for (w[0] = i, i = i + 1, k = 1; i < n; ++i) { int si = r[i].qs, ei = r[i].qe; if (r[i].id != r[i].parent) continue; // only check primary for (j = 0; j < k; ++j) { int sj = r[w[j]].qs, ej = r[w[j]].qe; int min = ej - sj < ei - si? ej - sj : ei - si; int ol = si < sj? (ei < sj? 0 : ei < ej? ei - sj : ej - sj) : (ej < si? 0 : ej < ei? ej - si : ei - si); if (ol > mask_level * min) { r[i].parent = r[w[j]].parent; if (r[w[j]].subsc < r[i].score) r[w[j]].subsc = r[i].score; break; } } if (j == k) w[k++] = i; } kfree(km, w); } void mm_sync_regs(void *km, int n_regs, mm_reg1_t *regs) // keep mm_reg1_t::{id,parent} in sync; also reset id { int *tmp, i, max_id = -1, n_tmp, n_pri; if (n_regs <= 0) return; for (i = 0; i < n_regs; ++i) max_id = max_id > regs[i].id? max_id : regs[i].id; n_tmp = max_id + 1; tmp = (int*)kmalloc(km, n_tmp * sizeof(int)); for (i = 0; i < n_tmp; ++i) tmp[i] = -1; for (i = 0; i < n_regs; ++i) if (regs[i].id >= 0) tmp[regs[i].id] = i; for (i = n_pri = 0; i < n_regs; ++i) { mm_reg1_t *r = ®s[i]; r->id = i; if (r->parent == MM_PARENT_TMP_PRI) r->parent = i; else if (r->parent >= 0 && tmp[r->parent] >= 0) r->parent = tmp[r->parent]; else r->parent = MM_PARENT_UNSET; if (r->id == r->parent) { if (n_pri++ == 0) r->sam_pri = 1; } } kfree(km, tmp); } void mm_select_sub(void *km, float mask_level, float pri_ratio, int *n_, mm_reg1_t *r) { if (pri_ratio > 0.0f && *n_ > 0) { int i, k, n = *n_; for (i = k = 0; i < n; ++i) if (r[i].parent == i || r[i].score >= r[r[i].parent].score * pri_ratio) r[k++] = r[i]; if (k != n) mm_sync_regs(km, k, r); // removing hits requires sync() *n_ = k; } } int mm_squeeze_a(void *km, int n_regs, mm_reg1_t *regs, mm128_t *a) { // squeeze out regions in a[] that are not referenced by regs[] int i, as = 0; uint64_t *aux; aux = (uint64_t*)kmalloc(km, n_regs * 8); for (i = 0; i < n_regs; ++i) aux[i] = (uint64_t)regs[i].as << 32 | i; radix_sort_64(aux, aux + n_regs); for (i = 0; i < n_regs; ++i) { mm_reg1_t *r = ®s[(int32_t)aux[i]]; if (r->as != as) { memmove(&a[as], &a[r->as], r->cnt * 16); r->as = as; } as += r->cnt; } kfree(km, aux); return as; } void mm_join_long(void *km, const mm_mapopt_t *opt, int qlen, int n_regs, mm_reg1_t *regs, mm128_t *a) { int i, n_aux; uint64_t *aux; if (n_regs < 2) return; // nothing to join mm_squeeze_a(km, n_regs, regs, a); aux = (uint64_t*)kmalloc(km, n_regs * 8); for (i = n_aux = 0; i < n_regs; ++i) if (regs[i].parent == i || regs[i].parent < 0) aux[n_aux++] = (uint64_t)regs[i].as << 32 | i; radix_sort_64(aux, aux + n_aux); for (i = n_aux - 1; i >= 1; --i) { mm_reg1_t *r0 = ®s[(int32_t)aux[i-1]], *r1 = ®s[(int32_t)aux[i]]; mm128_t *a0e, *a1s; int max_gap, min_gap; // test if (r0->as + r0->cnt != r1->as) continue; // not adjacent in a[] if (r0->rid != r1->rid || r0->rev != r1->rev) continue; // make sure on the same target and strand if (r0->score < opt->min_join_flank_sc || r1->score < opt->min_join_flank_sc) continue; // require good flanking chains a0e = &a[r0->as + r0->cnt - 1]; a1s = &a[r1->as]; if (a1s->x <= a0e->x || (int32_t)a1s->y <= (int32_t)a0e->y) continue; // keep colinearity max_gap = min_gap = (int32_t)a1s->y - (int32_t)a0e->y; max_gap = max_gap > a1s->x - a0e->x? max_gap : a1s->x - a0e->x; min_gap = min_gap < a1s->x - a0e->x? min_gap : a1s->x - a0e->x; if (max_gap > opt->max_join_long || min_gap > opt->max_join_short) continue; // all conditions satisfied; join a[r1->as].y |= 1ULL<<40; r0->cnt += r1->cnt, r0->score += r1->score; mm_reg_set_coor(r0, qlen, a); r1->cnt = 0; } kfree(km, aux); } void mm_set_mapq(int n_regs, mm_reg1_t *regs) { int i; for (i = 0; i < n_regs; ++i) { mm_reg1_t *r = ®s[i]; if (r->parent == r->id) { int mapq; mapq = (int)(30.0 * (1. - (float)r->subsc / r->score) * logf(r->score)); mapq = mapq > 0? mapq : 0; r->mapq = mapq < 60? mapq : 60; } else r->mapq = 0; } }