var getopt = function(args, ostr) { var oli; // option letter list index if (typeof(getopt.place) == 'undefined') getopt.ind = 0, getopt.arg = null, getopt.place = -1; if (getopt.place == -1) { // update scanning pointer if (getopt.ind >= args.length || args[getopt.ind].charAt(getopt.place = 0) != '-') { getopt.place = -1; return null; } if (getopt.place + 1 < args[getopt.ind].length && args[getopt.ind].charAt(++getopt.place) == '-') { // found "--" ++getopt.ind; getopt.place = -1; return null; } } var optopt = args[getopt.ind].charAt(getopt.place++); // character checked for validity if (optopt == ':' || (oli = ostr.indexOf(optopt)) < 0) { if (optopt == '-') return null; // if the user didn't specify '-' as an option, assume it means null. if (getopt.place < 0) ++getopt.ind; return '?'; } if (oli+1 >= ostr.length || ostr.charAt(++oli) != ':') { // don't need argument getopt.arg = null; if (getopt.place < 0 || getopt.place >= args[getopt.ind].length) ++getopt.ind, getopt.place = -1; } else { // need an argument if (getopt.place >= 0 && getopt.place < args[getopt.ind].length) getopt.arg = args[getopt.ind].substr(getopt.place); else if (args.length <= ++getopt.ind) { // no arg getopt.place = -1; if (ostr.length > 0 && ostr.charAt(0) == ':') return ':'; return '?'; } else getopt.arg = args[getopt.ind]; // white space getopt.place = -1; ++getopt.ind; } return optopt; } var c, pri_only = false; while ((c = getopt(arguments, "p")) != null) if (c == 'p') pri_only = true; var file = arguments.length == getopt.ind? new File() : new File(arguments[getopt.ind]); var buf = new Bytes(); var re = /(\d+)([MIDSHNX=])/g; var len = {}, lineno = 0; while (file.readline(buf) >= 0) { var m, n_cigar = 0, line = buf.toString(); ++lineno; if (line.charAt(0) == '@') { if (/^@SQ/.test(line)) { var name = (m = /\tSN:(\S+)/.exec(line)) != null? m[1] : null; var l = (m = /\tLN:(\d+)/.exec(line)) != null? parseInt(m[1]) : null; if (name != null && l != null) len[name] = l; } continue; } var t = line.split("\t"); var flag = parseInt(t[1]); if (t[9] != '*' && t[10] != '*' && t[9].length != t[10].length) throw Error("ERROR at line " + lineno + ": inconsistent SEQ and QUAL lengths - " + t[9].length + " != " + t[10].length); if (t[2] == '*' || (flag&4)) continue; if (pri_only && (flag&0x100)) continue; var tlen = len[t[2]]; if (tlen == null) throw Error("ERROR at line " + lineno + ": can't find the length of contig " + t[2]); var nn = (m = /\tnn:i:(\d+)/.exec(line)) != null? parseInt(m[1]) : 0; var NM = (m = /\tNM:i:(\d+)/.exec(line)) != null? parseInt(m[1]) : null; var have_NM = NM == null? false : true; NM += nn; var clip = [0, 0], I = [0, 0], D = [0, 0], M = 0, N = 0, ql = 0, tl = 0, mm = 0, ext_cigar = false; while ((m = re.exec(t[5])) != null) { var l = parseInt(m[1]); if (m[2] == 'M') M += l, ql += l, tl += l, ext_cigar = false; else if (m[2] == 'I') ++I[0], I[1] += l, ql += l; else if (m[2] == 'D') ++D[0], D[1] += l, tl += l; else if (m[2] == 'N') N += l, tl += l; else if (m[2] == 'S') clip[M == 0? 0 : 1] = l, ql += l; else if (m[2] == 'H') clip[M == 0? 0 : 1] = l; else if (m[2] == '=') M += l, ql += l, tl += l, ext_cigar = true; else if (m[2] == 'X') M += l, ql += l, tl += l, mm += l, ext_cigar = true; ++n_cigar; } if (n_cigar > 65535) warn("WARNING at line " + lineno + ": " + n_cigar + " CIGAR operations"); if (tl + parseInt(t[3]) - 1 > tlen) { warn("WARNING at line " + lineno + ": alignment end position larger than ref length; skipped"); continue; } if (t[9] != '*' && t[9].length != ql) { warn("WARNING at line " + lineno + ": SEQ length inconsistent with CIGAR (" + t[9].length + " != " + ql + "); skipped"); continue; } if (!have_NM || ext_cigar) NM = I[1] + D[1] + mm; if (NM < I[1] + D[1] + mm) { warn("WARNING at line " + lineno + ": NM is less than the total number of gaps (" + NM + " < " + (I[1]+D[1]+mm) + ")"); NM = I[1] + D[1] + mm; } var extra = ["mm:i:"+(NM-I[1]-D[1]), "io:i:"+I[0], "in:i:"+I[1], "do:i:"+D[0], "dn:i:"+D[1]]; var match = M - (NM - I[1] - D[1]); var blen = M + I[1] + D[1]; var qlen = M + I[1] + clip[0] + clip[1]; var qs, qe; if (flag&16) qs = clip[1], qe = qlen - clip[0]; else qs = clip[0], qe = qlen - clip[1]; var ts = parseInt(t[3]) - 1, te = ts + M + D[1] + N; var a = [t[0], qlen, qs, qe, flag&16? '-' : '+', t[2], tlen, ts, te, match, blen, t[4]]; print(a.join("\t"), extra.join("\t")); } buf.destroy(); file.close();