var getopt = function(args, ostr) { var oli; // option letter list index if (typeof(getopt.place) == 'undefined') getopt.ind = 0, getopt.arg = null, getopt.place = -1; if (getopt.place == -1) { // update scanning pointer if (getopt.ind >= args.length || args[getopt.ind].charAt(getopt.place = 0) != '-') { getopt.place = -1; return null; } if (getopt.place + 1 < args[getopt.ind].length && args[getopt.ind].charAt(++getopt.place) == '-') { // found "--" ++getopt.ind; getopt.place = -1; return null; } } var optopt = args[getopt.ind].charAt(getopt.place++); // character checked for validity if (optopt == ':' || (oli = ostr.indexOf(optopt)) < 0) { if (optopt == '-') return null; // if the user didn't specify '-' as an option, assume it means null. if (getopt.place < 0) ++getopt.ind; return '?'; } if (oli+1 >= ostr.length || ostr.charAt(++oli) != ':') { // don't need argument getopt.arg = null; if (getopt.place < 0 || getopt.place >= args[getopt.ind].length) ++getopt.ind, getopt.place = -1; } else { // need an argument if (getopt.place >= 0 && getopt.place < args[getopt.ind].length) getopt.arg = args[getopt.ind].substr(getopt.place); else if (args.length <= ++getopt.ind) { // no arg getopt.place = -1; if (ostr.length > 0 && ostr.charAt(0) == ':') return ':'; return '?'; } else getopt.arg = args[getopt.ind]; // white space getopt.place = -1; ++getopt.ind; } return optopt; } var c, fn_ucsc_fai = null; while ((c = getopt(arguments, "u:")) != null) { if (c == 'u') fn_ucsc_fai = getopt.arg; } if (getopt.ind == arguments.length) { print("Usage: k8 gff2bed.js [-u ucsc-genome.fa.fai] "); exit(1); } var ens2ucsc = {}; if (fn_ucsc_fai != null) { var buf = new Bytes(); var file = new File(fn_ucsc_fai); while (file.readline(buf) >= 0) { var t = buf.toString().split("\t"); var s = t[0]; if (/_(random|alt)$/.test(s)) { s = s.replace(/_(random|alt)$/, ''); s = s.replace(/^chr\S+_/, ''); } else { s = s.replace(/^chrUn_/, ''); } s = s.replace(/v(\d+)/, ".$1"); if (s != t[0]) ens2ucsc[s] = t[0]; } file.close(); buf.destroy(); } function print_bed12(exons, cds_st, cds_en) { if (exons.length == 0) return; var name = exons[0].slice(4, 7).join("|"); var a = exons.sort(function(a,b) {return a[1]-b[1]}); var sizes = [], starts = [], st, en; st = a[0][1]; en = a[a.length - 1][2]; if (cds_st == 1<<30) cds_st = st; if (cds_en == 0) cds_en = en; if (cds_st < st || cds_en > en) throw Error("inconsistent thick start or end for transcript " + a[0][4]); for (var i = 0; i < a.length; ++i) { sizes.push(a[i][2] - a[i][1]); starts.push(a[i][1]); } print(a[0][0], st, en, name, 0, a[0][3], cds_st, cds_en, ".", a.length, sizes.join(",") + ",", starts.join(",") + ","); } var re_gtf = /(transcript_id|transcript_type|transcript_biotype|gene_name) "([^"]+)";/g; var re_gff3 = /(transcript_id|transcript_type|transcript_biotype|gene_name)=([^;]+)/g; var buf = new Bytes(); var file = new File(arguments[getopt.ind]); var exons = [], cds_st = 1<<30, cds_en = 0, last_id = null; while (file.readline(buf) >= 0) { var t = buf.toString().split("\t"); if (t[0].charAt(0) == '#') continue; if (t[2] != "CDS" && t[2] != "exon") continue; t[3] = parseInt(t[3]) - 1; t[4] = parseInt(t[4]); var id = null, type = "", gname = "N/A", biotype = "", m; while ((m = re_gtf.exec(t[8])) != null) { if (m[1] == "transcript_id") id = m[2]; else if (m[1] == "transcript_type") type = m[2]; else if (m[1] == "transcript_biotype") biotype = m[2]; else if (m[1] == "gene_name") name = m[2]; } while ((m = re_gff3.exec(t[8])) != null) { if (m[1] == "transcript_id") id = m[2]; else if (m[1] == "transcript_type") type = m[2]; else if (m[1] == "transcript_biotype") biotype = m[2]; else if (m[1] == "gene_name") name = m[2]; } if (type == "" && biotype != "") type = biotype; if (id == null) throw Error("No transcript_id"); if (id != last_id) { print_bed12(exons, cds_st, cds_en); exons = [], cds_st = 1<<30, cds_en = 0; last_id = id; } if (t[2] == "CDS") { cds_st = cds_st < t[3]? cds_st : t[3]; cds_en = cds_en > t[4]? cds_en : t[4]; } else if (t[2] == "exon") { if (fn_ucsc_fai != null && ens2ucsc[t[0]] != null) t[0] = ens2ucsc[t[0]]; exons.push([t[0], t[3], t[4], t[6], id, type, name]); } } if (last_id != null) print_bed12(exons, cds_st, cds_en); file.close(); buf.destroy();