diff --git a/README.md b/README.md
index 21f69f0..50bdc94 100644
--- a/README.md
+++ b/README.md
@@ -10,6 +10,8 @@ cd minimap2 && make
 ./minimap2 -ax map10k MT-human.mmi test/MT-orang.fa > test.sam
 # long-read overlap (no test data)
 ./minimap2 -x ava-pb your-reads.fa your-reads.fa > overlaps.paf
+# spliced alignment (no test data)
+./minimap2 -ax splice ref.fa rna-seq-reads.fa > spliced.sam
 # man page
 man ./minimap2.1
 ```