Documented ov-eval.js
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@ -17,7 +17,8 @@ minimap2 -cx splice ref.fa rna-seq.fq | splice2bed.js - # convert splice aln t
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- [Convert SAM to PAF](#sam2paf)
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- [Convert GTF/GFF3 to BED12 format](#gff2bed)
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- [Convert spliced alignment to BED12](#splice2bed)
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- [Convert spliced alignment to BED12](#eval)
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- [Evaluating mapping accuracy with simulated reads](#mapeval)
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- [Evaluating read overlap sensitivity](#oveval)
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## <a name="intro"></a>Introduction
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@ -54,7 +55,7 @@ seamlessly works with both GTF and GFF3.
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Script [splice2bed.js](splice2bed.js) converts spliced alignment in SAM or PAF
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to 12-column BED format.
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### <a name="eval"></a>Evaluating mapping accuracy with simulated reads
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### <a name="mapeval"></a>Evaluating mapping accuracy with simulated reads
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Script [sim-pbsim.js](sim-pbsim.js) converts the MAF output of [pbsim][pbsim]
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to FASTQ and encodes the true mapping position in the read name in a format like
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@ -85,6 +86,11 @@ mappings, accumulative mapping error rate and the accumulative number of
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mapped reads. The U-line gives the number of unmapped reads if they are present
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in the SAM file.
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### <a name="oveval"></a>Evaluating read overlap sensitivity
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Script [ov-eval.js](ov-eval.js) takes read-to-reference alignment in PAF and
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read overlaps in PAF and evaluates the sensitivity.
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[cs]: https://github.com/lh3/minimap2#cs
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[k8]: https://github.com/attractivechaos/k8
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[maf]: https://genome.ucsc.edu/FAQ/FAQformat#format5
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@ -1,3 +1,5 @@
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#!/usr/bin/env k8
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var getopt = function(args, ostr) {
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var oli; // option letter list index
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if (typeof(getopt.place) == 'undefined')
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