updated manpage
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minimap2.1
19
minimap2.1
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@ -364,6 +364,17 @@ on SIRV data, please add
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.B --splice-flank=no
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.B --splice-flank=no
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to the command line.
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to the command line.
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.TP
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.TP
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.BR --junc-bed \ FILE
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BED file consisting of annotated introns and their strands. With this option,
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minimap2 prefers splicing in annotations.
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.I FILE
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can be generated with `paftools.js gff2bed -j ann.gtf' [].
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.TP
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.BR --junc-bonus \ INT
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Score bonus for a splice donor or acceptor found in annotation (effective with
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.BR --junc-bed )
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[0].
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.TP
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.BI --end-seed-pen \ INT
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.BI --end-seed-pen \ INT
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Drop a terminal anchor if
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Drop a terminal anchor if
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.IR s <log( g )+ INT ,
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.IR s <log( g )+ INT ,
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@ -507,7 +518,7 @@ is determined by the sequencing error mode.
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.B asm5
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.B asm5
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Long assembly to reference mapping
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Long assembly to reference mapping
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.RB ( -k19
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.RB ( -k19
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.B -w19 -A1 -B19 -O39,81 -E3,1 -s200 -z200
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.B -w19 -A1 -B19 -O39,81 -E3,1 -s200 -z200 -N50
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.BR --min-occ-floor=100 ).
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.BR --min-occ-floor=100 ).
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Typically, the alignment will not extend to regions with 5% or higher sequence
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Typically, the alignment will not extend to regions with 5% or higher sequence
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divergence. Only use this preset if the average divergence is far below 5%.
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divergence. Only use this preset if the average divergence is far below 5%.
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@ -515,14 +526,14 @@ divergence. Only use this preset if the average divergence is far below 5%.
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.B asm10
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.B asm10
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Long assembly to reference mapping
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Long assembly to reference mapping
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.RB ( -k19
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.RB ( -k19
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.B -w19 -A1 -B9 -O16,41 -E2,1 -s200 -z200
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.B -w19 -A1 -B9 -O16,41 -E2,1 -s200 -z200 -N50
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.BR --min-occ-floor=100 ).
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.BR --min-occ-floor=100 ).
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Up to 10% sequence divergence.
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Up to 10% sequence divergence.
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.TP
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.TP
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.B asm20
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.B asm20
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Long assembly to reference mapping
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Long assembly to reference mapping
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.RB ( -k19
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.RB ( -k19
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.B -w10 -A1 -B4 -O6,26 -E2,1 -s200 -z200
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.B -w10 -A1 -B4 -O6,26 -E2,1 -s200 -z200 -N50
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.BR --min-occ-floor=100 ).
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.BR --min-occ-floor=100 ).
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Up to 20% sequence divergence.
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Up to 20% sequence divergence.
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.TP
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.TP
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@ -544,7 +555,7 @@ is that this preset is not using HPC minimizers.
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.B splice
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.B splice
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Long-read spliced alignment
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Long-read spliced alignment
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.RB ( -k15
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.RB ( -k15
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.B -w5 --splice -g2000 -G200k -A1 -B2 -O2,32 -E1,0 -C9 -z200 -ub
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.B -w5 --splice -g2000 -G200k -A1 -B2 -O2,32 -E1,0 -C9 -z200 -ub --junc-bonus=9
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.BR --splice-flank=yes ).
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.BR --splice-flank=yes ).
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In the splice mode, 1) long deletions are taken as introns and represented as
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In the splice mode, 1) long deletions are taken as introns and represented as
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the
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the
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