When run without an explicit -onlyCoding option, as we've been doing up to now, snpEff automatically sets -onlyCoding to "true" provided that there is at least one transcript marked as "protein_coding", which will always be the case for us in practice (and indeed, all pipeline runs so far with snpEff 2.0.5 have run with -onlyCoding auto-set to "true"). However, given the disastrous effect on annotation quality setting "-onlyCoding false" has, we wish to be explicit with this option rather than relying on snpEff's auto-detection logic. |
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