#!/usr/bin/env Rscript args <- commandArgs(TRUE) base_name = args[1] input = args[2] d <- read.table(input, header=T) # separate the data into filtered and unfiltered d.filtered <- d[d$filter_type=="filtered",] d.unfiltered <- d[d$filter_type=="unfiltered",] if (nrow(d.filtered) > 0) { d.display <- d.filtered } else { d.display <- d.unfiltered } # # Plot histograms of the known versus novel Ti/Tv # outfile = paste(base_name, ".histograms.png", sep="") if (nrow(d.filtered) > 0) { nFilterTypes <- 2 } else { nFilterTypes <- 1 } bitmap(outfile, width=600, height=(300 * nFilterTypes), units="px") par(cex=1.1, mfrow=c(1 * nFilterTypes,2)) nbreaks <- 20 color <- "grey" xlim <- c(0,4) hist(d.unfiltered$known_titv, nbreaks, col=color, xlim=xlim) hist(d.unfiltered$novel_titv, nbreaks, col=color, xlim=xlim) if (nrow(d.filtered) > 0) { hist(d.filtered$known_titv, nbreaks, col=color, xlim=xlim) hist(d.filtered$novel_titv, nbreaks, col=color, xlim=xlim) } dev.off() # # Plot samples in order of novel Ti/Tv versus known Ti/Tv # outfile = paste(base_name, ".novel_vs_known_titv.png", sep="") bitmap(outfile, width=600, height=600, units="px") d.display <- d.display[order(d.display$novel_titv),] plot(1:length(d.display$known_titv),d.display$known_titv,type="b",col="blue",ylim=c(0,4), xlab="Sample #", ylab="Ti / Tv") points(1:length(d.display$novel_titv),d.display$novel_titv,type="b",col="red",ylim=c(0,4)) legend("bottomright", c("known","novel"), col=c("blue","red"), pch=21) dev.off()