/* * Copyright (c) 2010 The Broad Institute * Permission is hereby granted, free of charge, to any person * obtaining a copy of this software and associated documentation * files (the ÓSoftwareÓ), to deal in the Software without * restriction, including without limitation the rights to use, * copy, modify, merge, publish, distribute, sublicense, and/or sell * copies of the Software, and to permit persons to whom the * Software is furnished to do so, subject to the following * conditions: * The above copyright notice and this permission notice shall be * included in all copies or substantial portions of the Software. * * THE SOFTWARE IS PROVIDED ÓAS ISÓ, WITHOUT WARRANTY OF ANY KIND, * EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES * OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND * NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT * HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, * WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING * FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR * THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ package org.broadinstitute.sting.utils.sam; import net.sf.samtools.*; import java.util.Map; import java.util.HashMap; import java.io.File; /** * A miscellaneous collection of utilities for working with SAM files, headers, etc. * Static methods only, please. * * @author mhanna * @version 0.1 */ public class ReadUtils { private ReadUtils() { } public static SAMFileHeader copySAMFileHeader(SAMFileHeader toCopy) { SAMFileHeader copy = new SAMFileHeader(); copy.setSortOrder(toCopy.getSortOrder()); copy.setGroupOrder(toCopy.getGroupOrder()); copy.setProgramRecords(toCopy.getProgramRecords()); copy.setReadGroups(toCopy.getReadGroups()); copy.setSequenceDictionary(toCopy.getSequenceDictionary()); for (Map.Entry e : toCopy.getAttributes()) copy.setAttribute(e.getKey(), e.getValue()); return copy; } public static SAMFileWriter createSAMFileWriterWithCompression(SAMFileHeader header, boolean presorted, String file, int compression) { if (file.endsWith(".bam")) return new SAMFileWriterFactory().makeBAMWriter(header, presorted, new File(file), compression); return new SAMFileWriterFactory().makeSAMOrBAMWriter(header, presorted, new File(file)); } public static boolean isPlatformRead(SAMRecord read, String name) { SAMReadGroupRecord readGroup = read.getReadGroup(); if (readGroup != null) { Object readPlatformAttr = readGroup.getAttribute("PL"); if (readPlatformAttr != null) return readPlatformAttr.toString().toUpperCase().contains(name); } return false; } public static boolean is454Read(SAMRecord read) { return isPlatformRead(read, "454"); } public static boolean isSOLiDRead(SAMRecord read) { return isPlatformRead(read, "SOLID"); } public static boolean isSLXRead(SAMRecord read) { return isPlatformRead(read, "ILLUMINA"); } private static final Map readFlagNames = new HashMap(); static { readFlagNames.put(0x1, "Paired"); readFlagNames.put(0x2, "Proper"); readFlagNames.put(0x4, "Unmapped"); readFlagNames.put(0x8, "MateUnmapped"); readFlagNames.put(0x10, "Forward"); //readFlagNames.put(0x20, "MateForward"); readFlagNames.put(0x4, "FirstOfPair"); readFlagNames.put(0x8, "SecondOfPair"); readFlagNames.put(0x100, "NotPrimary"); readFlagNames.put(0x200, "NON-PF"); readFlagNames.put(0x400, "Duplicate"); } public static String readFlagsAsString(SAMRecord rec) { String flags = ""; for (int flag : readFlagNames.keySet()) { if ((rec.getFlags() & flag) != 0) { flags += readFlagNames.get(flag) + " "; } } return flags; } }