#!/util/bin/perl $|++; use strict; if (scalar(@ARGV) != 1 ) { die("usage vcf_to_germline_maflite.pl \n"); } my ($normalAlias) = @ARGV; my $line; while (defined($line = )) { if ($line =~ /#/) { next; } my ($chrom, $pos, $rsName, $ref, $alt, $qual, $filter, $info, $format, $sampleA) = split("\t", $line); my ($genoNum) = split(":", $sampleA); my ($geno1, $geno2) = split("/",$genoNum); my $allele1 = "N"; my $allele2 = "N"; if ( $geno1 eq "0") { $allele1 = $ref; } elsif ($geno1 eq "1") { $allele1 = $alt; } elsif ($geno1 eq ".") { # do nothing - just leave the N in place } else { die("allele1: only handled single alt currently:\n$line \n"); } if ( $geno2 eq "0") { $allele2 = $ref; } elsif ($geno2 eq "1") { $allele2 = $alt; } elsif ($geno2 eq ".") { # do nothing - just leave the N in place } else { die("allele2: only handled single alt currently:\n$line\n"); } my $ncbiBuild = "36"; print join("\t", ($ncbiBuild, $chrom, $pos, $pos, $ref, $allele1, $allele2, $normalAlias, $normalAlias)) . "\n"; }