package org.broadinstitute.sting.alignment; import org.broadinstitute.sting.utils.cmdLine.Argument; import org.broadinstitute.sting.gatk.walkers.ReadWalker; import org.broadinstitute.sting.alignment.bwa.c.BWACAligner; import org.broadinstitute.sting.alignment.bwa.c.BWACConfiguration; import net.sf.samtools.SAMRecord; import java.util.Random; import java.util.Iterator; /** * Align reads to the reference specified by BWTPrefix. * * @author mhanna * @version 0.1 */ public class AlignmentWalker extends ReadWalker { @Argument(fullName="BWTPrefix",shortName="BWT",doc="Index files generated by bwa index -d bwtsw",required=false) String prefix = "/Users/mhanna/reference/Ecoli/Escherichia_coli_K12_MG1655.fasta"; /** * The actual aligner. */ private BWACAligner aligner = null; /** * A random number generator, used to generate alignments. */ private Random random = new Random(); /** * Create an aligner object. The aligner object will load and hold the BWT until close() is called. */ @Override public void initialize() { BWACConfiguration configuration = new BWACConfiguration(prefix); aligner = new BWACAligner(configuration); } /** * Aligns a read to the given reference. * @param ref Reference over the read. Read will most likely be unmapped, so ref will be null. * @param read Read to align. * @return Number of alignments found for this read. */ @Override public Integer map(char[] ref, SAMRecord read) { Iterator alignedReads = aligner.align(read); if(alignedReads.hasNext()) { SAMRecord[] bestAlignments = alignedReads.next(); SAMRecord selectedRead = bestAlignments[random.nextInt(bestAlignments.length)]; out.println(selectedRead.format()); return bestAlignments.length; } else { out.println(read.format()); return 0; } } /** * Initial value for reduce. In this case, alignments will be counted. * @return 0, indicating no alignments yet found. */ @Override public Integer reduceInit() { return 0; } /** * Calculates the number of alignments found. * @param value Number of alignments found by this map. * @param sum Number of alignments found before this map. * @return Number of alignments found up to and including this map. */ @Override public Integer reduce(Integer value, Integer sum) { return value + sum; } /** * Cleanup. * @param result Number of reads processed. */ @Override public void onTraversalDone(Integer result) { aligner.close(); super.onTraversalDone(result); } }