#ifndef BWTALN_H #define BWTALN_H #include #include "bwt.h" #define BWA_TYPE_NO_MATCH 0 #define BWA_TYPE_UNIQUE 1 #define BWA_TYPE_REPEAT 2 #define BWA_TYPE_MATESW 3 #define SAM_FPD 1 // paired #define SAM_FPP 2 // properly paired #define SAM_FSU 4 // self-unmapped #define SAM_FMU 8 // mate-unmapped #define SAM_FSR 16 // self on the reverse strand #define SAM_FMR 32 // mate on the reverse strand #define SAM_FR1 64 // this is read one #define SAM_FR2 128 // this is read two #define SAM_FSC 256 // secondary alignment #define BWA_AVG_ERR 0.02 #define BWA_MIN_RDLEN 35 // for read trimming #ifndef bns_pac #define bns_pac(pac, k) ((pac)[(k)>>2] >> ((~(k)&3)<<1) & 3) #endif typedef struct { bwtint_t w; int bid; } bwt_width_t; typedef struct { uint32_t n_mm:8, n_gapo:8, n_gape:8, a:1; bwtint_t k, l; int score; } bwt_aln1_t; typedef struct { char *name; ubyte_t *seq, *rseq, *qual; uint32_t len:20, strand:1, type:2, dummy:1, extra_flag:8; uint32_t n_mm:8, n_gapo:8, n_gape:8, mapQ:8; int score; // alignments in SA coordinates int n_aln; bwt_aln1_t *aln; // alignment information bwtint_t sa, pos; uint64_t c1:28, c2:28, seQ:8; // number of top1 and top2 hits; single-end mapQ int n_cigar; uint16_t *cigar; // for multi-threading only int tid; // NM and MD tags uint32_t full_len:20, nm:12; char *md; } bwa_seq_t; #define BWA_MODE_GAPE 0x01 #define BWA_MODE_COMPREAD 0x02 #define BWA_MODE_LOGGAP 0x04 #define BWA_MODE_NONSTOP 0x10 typedef struct { int s_mm, s_gapo, s_gape; int mode; int indel_end_skip, max_del_occ, max_entries; float fnr; int max_diff, max_gapo, max_gape; int max_seed_diff, seed_len; int n_threads; int max_top2; int trim_qual; } gap_opt_t; #define BWA_PET_STD 1 #define BWA_PET_SOLID 2 typedef struct { int max_isize; int max_occ; int type, is_sw; } pe_opt_t; struct __bwa_seqio_t; typedef struct __bwa_seqio_t bwa_seqio_t; #ifdef __cplusplus extern "C" { #endif gap_opt_t *gap_init_opt(); void bwa_aln_core(const char *prefix, const char *fn_fa, const gap_opt_t *opt); bwa_seqio_t *bwa_seq_open(const char *fn); void bwa_seq_close(bwa_seqio_t *bs); void seq_reverse(int len, ubyte_t *seq, int is_comp); bwa_seq_t *bwa_read_seq(bwa_seqio_t *seq, int n_needed, int *n, int is_comp, int trim_qual); void bwa_free_read_seq(int n_seqs, bwa_seq_t *seqs); int bwa_cal_maxdiff(int l, double err, double thres); void bwa_cs2nt_core(bwa_seq_t *p, bwtint_t l_pac, ubyte_t *pac); void bwa_cal_sa_reg_gap(int tid, bwt_t *const bwt[2], int n_seqs, bwa_seq_t *seqs, const gap_opt_t *opt); #ifdef __cplusplus } #endif #endif