/*
* Copyright (c) 2009 The Broad Institute
*
* Permission is hereby granted, free of charge, to any person
* obtaining a copy of this software and associated documentation
* files (the "Software"), to deal in the Software without
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* copy, modify, merge, publish, distribute, sublicense, and/or sell
* copies of the Software, and to permit persons to whom the
* Software is furnished to do so, subject to the following
* conditions:
*
* The above copyright notice and this permission notice shall be
* included in all copies or substantial portions of the Software.
*
* THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND,
* EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES
* OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND
* NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT
* HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY,
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* OTHER DEALINGS IN THE SOFTWARE.
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package org.broadinstitute.sting.gatk.traversals;
import net.sf.picard.filter.FilteringIterator;
import net.sf.picard.filter.SamRecordFilter;
import net.sf.samtools.SAMRecord;
import org.apache.log4j.Logger;
import org.broadinstitute.sting.gatk.contexts.AlignmentContext;
import org.broadinstitute.sting.gatk.datasources.providers.ReadView;
import org.broadinstitute.sting.gatk.datasources.providers.ReadShardDataProvider;
import org.broadinstitute.sting.gatk.iterators.PushbackIterator;
import org.broadinstitute.sting.gatk.walkers.DuplicateWalker;
import org.broadinstitute.sting.utils.GenomeLoc;
import org.broadinstitute.sting.utils.GenomeLocParser;
import org.broadinstitute.sting.utils.pileup.ReadBackedPileup;
import java.util.*;
/**
* @author Mark DePristo
* @version 0.1
*
* Class TraverseDuplicates
*
* This class handles traversing lists of duplicate reads in the new shardable style
*/
public class TraverseDuplicates extends TraversalEngine,ReadShardDataProvider> {
/** our log, which we want to capture anything from this class */
protected static Logger logger = Logger.getLogger(TraverseDuplicates.class);
/** descriptor of the type */
private static final String DUPS_STRING = "dups";
/** Turn this to true to enable logger.debug output */
private final boolean DEBUG = false;
private List readsAtLoc(final SAMRecord read, PushbackIterator iter) {
GenomeLoc site = GenomeLocParser.createGenomeLoc(read);
ArrayList l = new ArrayList();
l.add(read);
for (SAMRecord read2 : iter) {
GenomeLoc site2 = GenomeLocParser.createGenomeLoc(read2);
// the next read starts too late
if (site2.getStart() != site.getStart()) {
iter.pushback(read2);
break;
} else {
l.add(read2);
}
}
return l;
}
/**
* Creates a set of lists of reads, where each list contains reads from the same underlying molecule according
* to their duplicate flag and their (and mate, if applicable) start/end positions.
*
* @param reads the list of reads to split into unique molecular samples
* @return
*/
protected Set> uniqueReadSets(List reads) {
Set> readSets = new HashSet>();
// for each read, find duplicates, and either add the read to its duplicate list or start a new one
for ( SAMRecord read : reads ) {
List readSet = findDuplicateReads(read, readSets);
if ( readSet == null ) {
readSets.add(new ArrayList(Arrays.asList(read))); // copy so I can add to the list
} else {
readSet.add(read);
}
}
return readSets;
}
/**
* Find duplicate reads for read in the set of unique reads. This is effective a duplicate marking algorithm,
* but it relies for safety's sake on the file itself being marked by a true duplicate marking algorithm. Pair
* and single-end read aware.
*
* @param read
* @param readSets
* @return The list of duplicate reads that read is a member of, or null if it's the only one of its kind
*/
protected List findDuplicateReads(SAMRecord read, Set> readSets ) {
if ( read.getReadPairedFlag() ) {
// paired
final GenomeLoc readMateLoc = GenomeLocParser.createGenomeLoc(read.getMateReferenceIndex(), read.getMateAlignmentStart(), read.getMateAlignmentStart());
for (List reads : readSets) {
SAMRecord key = reads.get(0);
// read and key start at the same place, and either the this read and the key
// share a mate location or the read is flagged as a duplicate
if ( read.getAlignmentStart() == key.getAlignmentStart() && key.getReadPairedFlag() && ( key.getDuplicateReadFlag() || read.getDuplicateReadFlag() ) ) {
// at least one has to be marked as a duplicate
final GenomeLoc keyMateLoc = GenomeLocParser.createGenomeLoc(key.getMateReferenceIndex(), key.getMateAlignmentStart(), key.getMateAlignmentStart());
if ( readMateLoc.compareTo(keyMateLoc) == 0 ) {
// we are at the same position as the dup and have the same mat pos, it's a dup
if (DEBUG) logger.debug(String.format(" => Adding read to dups list: %s %d %s vs. %s", read, reads.size(), readMateLoc, keyMateLoc));
return reads;
}
}
}
} else {
for (List reads : readSets) {
SAMRecord key = reads.get(0);
boolean v = (! key.getReadPairedFlag()) && read.getAlignmentStart() == key.getAlignmentStart() && ( key.getDuplicateReadFlag() || read.getDuplicateReadFlag() ) && read.getReadLength() == key.getReadLength();
//System.out.printf("%s %s %b %b %d %d %d %d => %b%n",
// read.getReadPairedFlag(), key.getReadPairedFlag(), read.getDuplicateReadFlag(), key.getDuplicateReadFlag(),
// read.getAlignmentStart(), key.getAlignmentStart(), read.getReadLength(), key.getReadLength(), v);
if ( v ) {
//System.out.printf("Returning reads...%n");
return reads;
}
}
}
return null;
}
/**
* Class to filter out un-handle-able reads from the stream. We currently are skipping
* unmapped reads, non-primary reads, unaligned reads, and duplicate reads.
*/
public static class duplicateStreamFilterFunc implements SamRecordFilter {
SAMRecord lastRead = null;
public boolean filterOut(SAMRecord rec) {
boolean result = false;
if (rec.getReadUnmappedFlag()) {
TraversalStatistics.nUnmappedReads++;
result = true;
} else if (rec.getNotPrimaryAlignmentFlag()) {
TraversalStatistics.nNotPrimary++;
result = true;
} else if (rec.getAlignmentStart() == SAMRecord.NO_ALIGNMENT_START) {
TraversalStatistics.nBadAlignments++;
result = true;
} else {
result = false;
}
if (result) {
TraversalStatistics.nSkippedReads++;
//System.out.printf(" [filter] %s => %b", rec.getReadName(), result);
} else {
TraversalStatistics.nReads++;
}
return result;
}
}
// --------------------------------------------------------------------------------------------------------------
//
// new style interface to the system
//
// --------------------------------------------------------------------------------------------------------------
/**
* Traverse by reads, given the data and the walker
*
* @param walker the walker to execute over
* @param sum of type T, the return from the walker
*
* @return the result type T, the product of all the reduce calls
*/
public T traverse(DuplicateWalker walker,
ReadShardDataProvider dataProvider,
T sum) {
FilteringIterator filterIter = new FilteringIterator(new ReadView(dataProvider).iterator(), new duplicateStreamFilterFunc());
PushbackIterator iter = new PushbackIterator(filterIter);
/**
* while we still have more reads:
* ok, here's the idea. We get all the reads that start at the same position in the genome
* We then split the list of reads into sublists of reads:
* -> those with the same mate pair position, for paired reads
* -> those flagged as unpaired and duplicated but having the same start and end
*/
for (SAMRecord read : iter) {
// get the genome loc from the read
GenomeLoc site = GenomeLocParser.createGenomeLoc(read);
Set> readSets = uniqueReadSets(readsAtLoc(read, iter));
if ( DEBUG ) logger.debug(String.format("*** TraverseDuplicates.traverse at %s with %d read sets", site, readSets.size()));
// Jump forward in the reference to this locus location
AlignmentContext locus = new AlignmentContext(site, new ReadBackedPileup(site));
// update the number of duplicate sets we've seen
TraversalStatistics.nRecords++;
// actually call filter and map, accumulating sum
final boolean keepMeP = walker.filter(site, locus, readSets);
if (keepMeP) {
M x = walker.map(site, locus, readSets);
sum = walker.reduce(x, sum);
}
printProgress(DUPS_STRING, site);
if (this.maximumIterations > 0 && TraversalStatistics.nRecords > this.maximumIterations) {
logger.warn(String.format(("Maximum number of duplicate sets encountered, terminating traversal " + TraversalStatistics.nRecords)));
break;
}
}
return sum;
}
/**
* Temporary override of printOnTraversalDone.
*
* @param sum Result of the computation.
*/
public void printOnTraversalDone(T sum) {
printOnTraversalDone(DUPS_STRING, sum);
}
}