package org.broadinstitute.sting.gatk.traversals; import net.sf.samtools.SAMRecord; import org.apache.log4j.Logger; import org.broadinstitute.sting.gatk.WalkerManager; import org.broadinstitute.sting.gatk.datasources.providers.*; import org.broadinstitute.sting.gatk.refdata.ReadMetaDataTracker; import org.broadinstitute.sting.gatk.walkers.DataSource; import org.broadinstitute.sting.gatk.walkers.ReadWalker; import org.broadinstitute.sting.utils.GenomeLocParser; /* * Copyright (c) 2009 The Broad Institute * * Permission is hereby granted, free of charge, to any person * obtaining a copy of this software and associated documentation * files (the "Software"), to deal in the Software without * restriction, including without limitation the rights to use, * copy, modify, merge, publish, distribute, sublicense, and/or sell * copies of the Software, and to permit persons to whom the * Software is furnished to do so, subject to the following * conditions: * * The above copyright notice and this permission notice shall be * included in all copies or substantial portions of the Software. * * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, * EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES * OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND * NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT * HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, * WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING * FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR * OTHER DEALINGS IN THE SOFTWARE. */ /** * @author aaron * @version 1.0 * @date Apr 24, 2009 *

* Class TraverseReads *

* This class handles traversing by reads in the new shardable style */ public class TraverseReads extends TraversalEngine,ReadShardDataProvider> { /** our log, which we want to capture anything from this class */ protected static Logger logger = Logger.getLogger(TraverseReads.class); /** descriptor of the type */ private static final String READS_STRING = "reads"; /** * Traverse by reads, given the data and the walker * * @param walker the walker to traverse with * @param dataProvider the provider of the reads data * @param sum the value of type T, specified by the walker, to feed to the walkers reduce function * @return the reduce variable of the read walker */ public T traverse(ReadWalker walker, ReadShardDataProvider dataProvider, T sum) { logger.debug(String.format("TraverseReads.traverse Covered dataset is %s", dataProvider)); if( !dataProvider.hasReads() ) throw new IllegalArgumentException("Unable to traverse reads; no read data is available."); boolean needsReferenceBasesP = WalkerManager.isRequired(walker, DataSource.REFERENCE_BASES); ReadView reads = new ReadView(dataProvider); ReadReferenceView reference = new ReadReferenceView(dataProvider); // get the reference ordered data ReadBasedReferenceOrderedView rodView = new ReadBasedReferenceOrderedView(dataProvider); // while we still have more reads for (SAMRecord read : reads) { // an array of characters that represent the reference char[] refSeq = null; // get the array of characters for the reference sequence, since we're a mapped read if (needsReferenceBasesP && !read.getReadUnmappedFlag() && dataProvider.hasReference()) refSeq = reference.getReferenceBases(read); // update the number of reads we've seen TraversalStatistics.nRecords++; TraversalStatistics.nReads++; // if the read is mapped, create a metadata tracker ReadMetaDataTracker tracker = (read.getReferenceIndex() >= 0) ? rodView.getReferenceOrderedDataForRead(read) : null; final boolean keepMeP = walker.filter(refSeq, read); if (keepMeP) { M x = walker.map(refSeq, read, tracker); // the tracker can be null sum = walker.reduce(x, sum); } printProgress(READS_STRING, (read.getReferenceIndex() == SAMRecord.NO_ALIGNMENT_REFERENCE_INDEX) ? null : GenomeLocParser.createGenomeLoc(read.getReferenceIndex(),read.getAlignmentStart())); } return sum; } /** * Temporary override of printOnTraversalDone. * TODO: Add some sort of TE.getName() function once all TraversalEngines are ported. * @param sum Result of the computation. */ public void printOnTraversalDone( T sum ) { printOnTraversalDone(READS_STRING, sum ); } }