import java.io.PrintWriter import org.broadinstitute.sting.queue.function.ListWriterFunction import org.broadinstitute.sting.queue.QScript import org.broadinstitute.sting.queue.extensions.gatk._ import org.broadinstitute.sting.utils.exceptions.ReviewedStingException /** * A pipeline for Queue that runs a custom walker outside of the GATK jar. * NOTE: This code is an unsupported example for soliciting feedback on how to improve Queue. * Future syntax will simplify running the GATK so please expect the syntax below to change significantly. */ class PrintReadsAcrossManySamples extends QScript { @Input(doc="The reference file for the bam files.", shortName="R") var referenceFile: File = null // NOTE: Do not initialize List, Set, or Option to null // as you won't be able to update the collection. // By default set: // List[T] = Nil // Set[T] = Set.empty[T] // Option[T] = None @Input(doc="One or more bam files.", shortName="I") var bamFile: File = _ @Input(doc="Name of the test case", fullName="test_case",required=false) var testCaseName: String = "." @Input(doc="Max number of bam files to process", fullName="max_bams",required=false) var maxBams = 1 @Input(doc="Step size",fullName="step_size",required=false) var stepSize = 1 def script = { var lines = scala.io.Source.fromFile(bamFile).getLines.map(new File(_)).toList for(numBams <- 1 to (maxBams min lines.size) by stepSize) { val basePath = "%s/%03d_bams".format(testCaseName,numBams) val writeBamList = new ListWriterFunction writeBamList.inputFiles = lines.take(numBams) writeBamList.listFile = new File(basePath+"/bams.list") add(writeBamList) // Create the function that we can run. val printreads = new PrintReads printreads.jobOutputFile = new File(basePath+"/PrintReads.out") printreads.input_file = List(writeBamList.listFile) printreads.reference_sequence = referenceFile printreads.out = new File("/dev/null") printreads.memoryLimit = 8 add(printreads) } } }