/* * Copyright (c) 2009 The Broad Institute * * Permission is hereby granted, free of charge, to any person * obtaining a copy of this software and associated documentation * files (the "Software"), to deal in the Software without * restriction, including without limitation the rights to use, * copy, modify, merge, publish, distribute, sublicense, and/or sell * copies of the Software, and to permit persons to whom the * Software is furnished to do so, subject to the following * conditions: * * The above copyright notice and this permission notice shall be * included in all copies or substantial portions of the Software. * * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, * EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES * OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND * NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT * HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, * WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING * FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR * OTHER DEALINGS IN THE SOFTWARE. */ package org.broadinstitute.sting.gatk.contexts; import org.broadinstitute.sting.gatk.datasources.sample.Sample; import org.broadinstitute.sting.utils.exceptions.ReviewedStingException; import org.broadinstitute.sting.utils.GenomeLoc; import org.broadinstitute.sting.utils.exceptions.UserException; import org.broadinstitute.sting.utils.pileup.*; import java.util.*; /** * Useful utilities for storing different AlignmentContexts * User: ebanks */ public class StratifiedAlignmentContext { // Definitions: // COMPLETE = full alignment context // FORWARD = reads on forward strand // REVERSE = reads on forward strand // public enum StratifiedContextType { COMPLETE, FORWARD, REVERSE } private StratifiedAlignmentContext() { // cannot be instantiated } /** * Returns a potentially derived subcontext containing only forward, reverse, or in fact all reads * in alignment context context. * * @param context * @param type * @return */ public static AlignmentContext stratify(AlignmentContext context, StratifiedContextType type) { switch(type) { case COMPLETE: return context; case FORWARD: return new AlignmentContext(context.getLocation(),context.getPileup().getPositiveStrandPileup()); case REVERSE: return new AlignmentContext(context.getLocation(),context.getPileup().getNegativeStrandPileup()); default: throw new ReviewedStingException("Unable to get alignment context for type = " + type); } } public static Map splitContextBySampleName(AlignmentContext context) { return splitContextBySampleName(context, null); } public static Map splitContextBySample(AlignmentContext context) { Map m = new HashMap(); for ( Map.Entry entry : splitContextBySampleName(context, null).entrySet() ) { m.put(new Sample(entry.getKey()), entry.getValue()); } return m; } /** * Splits the given AlignmentContext into a StratifiedAlignmentContext per sample, but referencd by sample name instead * of sample object. * * @param context the original pileup * * @return a Map of sample name to StratifiedAlignmentContext * **/ public static Map splitContextBySampleName(AlignmentContext context, String assumedSingleSample) { GenomeLoc loc = context.getLocation(); HashMap contexts = new HashMap(); for(String sample: context.getPileup().getSampleNames()) { ReadBackedPileup pileupBySample = context.getPileup().getPileupForSampleName(sample); // Don't add empty pileups to the split context. if(pileupBySample.size() == 0) continue; if(sample != null) contexts.put(sample, new AlignmentContext(loc, pileupBySample)); else { if(assumedSingleSample == null) { throw new UserException.ReadMissingReadGroup(pileupBySample.iterator().next().getRead()); } contexts.put(assumedSingleSample,new AlignmentContext(loc, pileupBySample)); } } return contexts; } public static Map splitContextBySampleName(ReadBackedPileup pileup, String assumedSingleSample) { return splitContextBySampleName(new AlignmentContext(pileup.getLocation(), pileup)); } public static AlignmentContext joinContexts(Collection contexts) { // validation GenomeLoc loc = contexts.iterator().next().getLocation(); boolean isExtended = contexts.iterator().next().basePileup instanceof ReadBackedExtendedEventPileup; for(AlignmentContext context: contexts) { if(!loc.equals(context.getLocation())) throw new ReviewedStingException("Illegal attempt to join contexts from different genomic locations"); if(isExtended != (context.basePileup instanceof ReadBackedExtendedEventPileup)) throw new ReviewedStingException("Illegal attempt to join simple and extended contexts"); } AlignmentContext jointContext; if(isExtended) { List pe = new ArrayList(); for(AlignmentContext context: contexts) { for(PileupElement pileupElement: context.basePileup) pe.add((ExtendedEventPileupElement)pileupElement); } jointContext = new AlignmentContext(loc, new ReadBackedExtendedEventPileupImpl(loc,pe)); } else { List pe = new ArrayList(); for(AlignmentContext context: contexts) { for(PileupElement pileupElement: context.basePileup) pe.add(pileupElement); } jointContext = new AlignmentContext(loc, new ReadBackedPileupImpl(loc,pe)); } return jointContext; } }