#this script produces the output to go in emails import subprocess import os import re import sys import getopt import sample_lister try: opts, args = getopt.getopt(sys.argv[1:], "dp:s:") except getopt.GetoptError, err: # print help information and exit: print str(err) # will print something like "option -a not recognized" usage() sys.exit(2) opts=dict(opts) givenname=opts['-s'] projname=opts['-p'] if '-d' in opts: dirname=opts['-d'] else: dirname="" class SampSet(sample_lister.SampleSet): def __init__(self, projectname, sampset, pathname): self.sampset=sampset self.pathname=pathname self.projectname=projectname sample_lister.SampleSet.__init__(self, projectname, sampset, pathname) def evalout(self): '''This produced the output that needs to go in the emails''' filename = "/humgen/gsa-firehose/firehose/firehose_output/trunk/Sample_Set/" + self.sampset +"/UnifiedGenotyper/"+ self.sampset+".filtered.eval" evalfile = open(filename, "r").read() annotations= ["all", "novel", "known", "snp_at_known_non_snps", "filtered"] variant=dict(zip(annotations, ('','','','',''))) ratio=dict(zip(annotations, ('','','','',''))) bpre=re.compile("all,summary,variant_counts +n bases covered +(\d+)") size=repr(bpre.search(evalfile).group(1)) bamsearch="find /humgen/gsa-firehose/firehose/firehose_output/trunk/Sample_Set/"+self.sampset+"/* -name \*.bam" bams = subprocess.Popen([bamsearch], shell=True, stdout=subprocess.PIPE).communicate()[0] sampno=bams.count("bam") bedsearch="find /humgen/gsa-firehose/firehose/firehose_output/trunk/Sample_Set/"+self.sampset+"/* -name \*filtered_indels.bed" beds = subprocess.Popen([bedsearch], shell=True, stdout=subprocess.PIPE).communicate()[0] vcf="/humgen/gsa-firehose/firehose/firehose_output/trunk/Sample_Set/"+self.sampset+"/UnifiedGenotyper/"+self.sampset+'.maf.annotated.vcf' for a in annotations: anregexv = re.compile(a + ",summary,variant_counts +variants +(\d+)") variant[a] = repr(anregexv.search(evalfile).group(1)) anregexr = re.compile(a + ",summary,transitions_transversions +ratio +(\d+.\d+)") ratio[a] = repr(anregexr.search(evalfile).group(1)) out1="Samples processed:"+repr(sampno)+"\n\n Target size: \t" +size+" bp \n\n\t\t\t\t\t Variants \t\t Ti/TV \n (true positives)\t All \t\t " +variant["all"]+ " \t\t " + ratio["all"] +" \n \t\t\t Known \t\t " +variant["known"]+ " \t\t " + ratio['known']+" \n \t\t\t Novel \t\t " +variant["novel"]+" \t\t " + ratio['novel']+ " \n*************************************************************************\n (false \tSNPS at known indels \t " +variant["snp_at_known_non_snps"]+"\t\t\t " + ratio['snp_at_known_non_snps']+ " \n positives) \t\t filtered \t " +variant["filtered"]+" \t\t " + ratio['filtered'] out2="\n\n\nSNP calls:"+vcf+"\n\nIndel-realigned Bam files:\n"+bams+"\nIndel calls:\n"+beds if self.pathname == '': print(out1+out2) else: filename=self.pathname+self.sampset+".emailtxt" putthere=open(filename, "w") putthere.write(out1+out2) target=SampSet(projname,givenname,dirname) target.evalout() #TODO: make this send the email when run #TODO: make this find the list of bams, bed files, and annotated vcfs.