import org.broadinstitute.sting.queue.QScript import org.broadinstitute.sting.queue.extensions.gatk._ /** * A pipeline for Queue that runs a custom walker outside of the GATK jar. * NOTE: This code is an unsupported example for soliciting feedback on how to improve Queue. * Future syntax will simplify running the GATK so please expect the syntax below to change significantly. */ class ExampleCustomWalker extends QScript { // The full packaged jar should be used. // You can build this jar via 'ant package' and then find it under // 'Sting/dist/packages/GenomeAnalysisTK-*/GenomeAnalysisTK.jar' @Input(doc="The path to the packaged GenomeAnalysisTK.jar file.", shortName="gatk") var gatkJar: File = null @Input(doc="The reference file for the bam files.", shortName="R") var referenceFile: File = null // NOTE: Do not initialize List, Set, or Option to null // as you won't be able to update the collection. // By default set: // List[T] = Nil // Set[T] = Set.empty[T] // Option[T] = None @Input(doc="One or more bam files.", shortName="I") var bamFiles: List[File] = Nil /** * In script, you create and then add() functions to the pipeline. */ def script = { val myClasses = "myClassDir" val customWalker = new CommandLineGATK { // Set the name of your walker, for example this will be passed as -T MyCustomWalker this.analysis_type = "MyCustomWalker" // NOTE: At this time, you still need to specify the GATK jar or the pipeline won't validate. this.jarFile = gatkJar override def javaExecutable = "org.broadinstitute.sting.gatk.CommandLineGATK" override def javaOpts = "%s -cp %s:%s".format(super.javaOpts, gatkJar, myClasses) } customWalker.reference_sequence = referenceFile customWalker.input_file = bamFiles // Add the newly created function to the pipeline. add(customWalker) } }