/* * Copyright (c) 2009 The Broad Institute * * Permission is hereby granted, free of charge, to any person * obtaining a copy of this software and associated documentation * files (the "Software"), to deal in the Software without * restriction, including without limitation the rights to use, * copy, modify, merge, publish, distribute, sublicense, and/or sell * copies of the Software, and to permit persons to whom the * Software is furnished to do so, subject to the following * conditions: * * The above copyright notice and this permission notice shall be * included in all copies or substantial portions of the Software. * * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, * EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES * OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND * NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT * HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, * WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING * FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR * OTHER DEALINGS IN THE SOFTWARE. */ package org.broadinstitute.sting.gatk.contexts; import org.broadinstitute.sting.utils.exceptions.ReviewedStingException; import org.broadinstitute.sting.utils.GenomeLoc; import org.broadinstitute.sting.utils.exceptions.UserException; import org.broadinstitute.sting.utils.pileup.*; import java.util.*; /** * Useful class for storing different AlignmentContexts * User: ebanks * Modified: chartl (split by read group) */ public class StratifiedAlignmentContext { // Definitions: // COMPLETE = full alignment context // FORWARD = reads on forward strand // REVERSE = reads on forward strand // public enum StratifiedContextType { COMPLETE, FORWARD, REVERSE } private GenomeLoc loc; private RBP basePileup = null; // // accessors // public GenomeLoc getLocation() { return loc; } public StratifiedAlignmentContext(GenomeLoc loc) { this(loc,null); } public StratifiedAlignmentContext(GenomeLoc loc, RBP pileup) { this.loc = loc; this.basePileup = pileup; } public AlignmentContext getContext(StratifiedContextType type) { switch(type) { case COMPLETE: return new AlignmentContext(loc,basePileup); case FORWARD: return new AlignmentContext(loc,basePileup.getPositiveStrandPileup()); case REVERSE: return new AlignmentContext(loc,basePileup.getNegativeStrandPileup()); default: throw new ReviewedStingException("Unable to get alignment context for type = " + type); } } /** * Splits the given AlignmentContext into a StratifiedAlignmentContext per sample. * * @param pileup the original pileup * * @return a Map of sample name to StratifiedAlignmentContext * **/ public static Map splitContextBySample(RBP pileup) { return splitContextBySample(pileup, null); } /** * Splits the given AlignmentContext into a StratifiedAlignmentContext per sample. * * @param pileup the original pileup * @param assumedSingleSample if not null, any read without a readgroup will be given this sample name * * @return a Map of sample name to StratifiedAlignmentContext * **/ public static Map splitContextBySample(RBP pileup, String assumedSingleSample) { GenomeLoc loc = pileup.getLocation(); HashMap contexts = new HashMap(); for(String sampleName: pileup.getSamples()) { RBP pileupBySample = (RBP)pileup.getPileupForSample(sampleName); // Don't add empty pileups to the split context. if(pileupBySample.size() == 0) continue; if(sampleName != null) contexts.put(sampleName,new StratifiedAlignmentContext(loc,pileupBySample)); else { if(assumedSingleSample == null) { throw new UserException.MalformedBam(pileupBySample.iterator().next().getRead(), "Missing read group for read"); } contexts.put(assumedSingleSample,new StratifiedAlignmentContext(loc,pileupBySample)); } } return contexts; } /** * Splits the given AlignmentContext into a StratifiedAlignmentContext per read group. * * @param pileup the original pileup * @return a Map of sample name to StratifiedAlignmentContext * TODO - support for collapsing or assuming read groups if they are missing * **/ public static Map> splitContextByReadGroup(RBP pileup) { HashMap> contexts = new HashMap>(); for(String readGroupId: pileup.getReadGroups()) contexts.put(readGroupId,new StratifiedAlignmentContext(pileup.getLocation(),(RBP)pileup.getPileupForReadGroup(readGroupId))); return contexts; } public static AlignmentContext joinContexts(Collection contexts) { // validation GenomeLoc loc = contexts.iterator().next().getLocation(); boolean isExtended = contexts.iterator().next().basePileup instanceof ReadBackedExtendedEventPileup; for(StratifiedAlignmentContext context: contexts) { if(!loc.equals(context.getLocation())) throw new ReviewedStingException("Illegal attempt to join contexts from different genomic locations"); if(isExtended != (context.basePileup instanceof ReadBackedExtendedEventPileup)) throw new ReviewedStingException("Illegal attempt to join simple and extended contexts"); } AlignmentContext jointContext; if(isExtended) { List pe = new ArrayList(); for(StratifiedAlignmentContext context: contexts) { for(PileupElement pileupElement: context.basePileup) pe.add((ExtendedEventPileupElement)pileupElement); } jointContext = new AlignmentContext(loc, new ReadBackedExtendedEventPileupImpl(loc,pe)); } else { List pe = new ArrayList(); for(StratifiedAlignmentContext context: contexts) { for(PileupElement pileupElement: context.basePileup) pe.add(pileupElement); } jointContext = new AlignmentContext(loc, new ReadBackedPileupImpl(loc,pe)); } return jointContext; } }