/* * The Broad Institute * SOFTWARE COPYRIGHT NOTICE AGREEMENT * This software and its documentation are copyright 2009 by the * Broad Institute/Massachusetts Institute of Technology. All rights are reserved. * * This software is supplied without any warranty or guaranteed support whatsoever. Neither * the Broad Institute nor MIT can be responsible for its use, misuse, or functionality. */ package org.broadinstitute.sting.secondarybase; import net.sf.picard.PicardException; import edu.mit.broad.picard.util.BasicTextFileParser; import net.sf.picard.util.FormatUtil; import java.io.File; /** * Class to parse the data in an Illumina Firecrest directory and return an iterator over that data, in order * by tile. * * @author Kiran Garimella */ public class FirecrestFileParser extends AbstractFirecrestFileParser { private BasicTextFileParser parser; private final FormatUtil formatter = new FormatUtil(); private final File[] intensityFiles; /** * Constructor * * @param firecrestDirectory directory where the Firecrest files can be located * @param lane the lane to parse */ public FirecrestFileParser(final File firecrestDirectory, final int lane) { super(firecrestDirectory, lane); intensityFiles = getFilesMatchingRegexp("s_" + lane + "_\\d{4}_int.txt(.gz)?"); } @Override public boolean isValidFirecrestDirectory() { return (intensityFiles.length > 0); } /** * Sorts the relevant files in the firecrestDirectory. Does some basic sanity checking to ensure that some files * are found and that they are the expected multiple for paired-end or not. */ @Override protected void prepareToIterate() { // Some basic sanity checking on file counts if (intensityFiles.length == 0) { throw new PicardException("No Firecrest 1.3 intensity files found in " + firecrestDirectory.getAbsolutePath() + " for lane " + lane); } // Sort each set of reads and create a text parser for it parser = makeParserForTextFiles(true, intensityFiles); } /** * Parses the next line from the parser and constructs a FirecrestReadData object from it * The first 4 fields are position information for the read, and the remaining value are * the intensities data. * * @return a fully populated FirecrestReadData object */ protected FirecrestReadData readNext() { if (!parser.hasNext()) { return null; } final String[] data = parser.next(); final int lane = formatter.parseInt(data[0]); final int tile = formatter.parseInt(data[1]); final int x = formatter.parseInt(data[2]); final int y = formatter.parseInt(data[3]); int intensityOffset = 4; int numIntensities = (data.length - 4)/4; double[][] intensities = new double[numIntensities][4]; for (int cycle = 0, index = intensityOffset; cycle < numIntensities; cycle++) { for (int channel = 0; channel < 4; channel++, index++) { intensities[cycle][channel] = formatter.parseFloat(data[index]); } } return new FirecrestReadData(lane, tile, x, y, intensities); } /** * Closes the underlying PasteParser */ @Override public void close() { if (parser != null) { parser.close(); } } }