package org.broadinstitute.sting.gatk;
import org.broadinstitute.sting.gatk.walkers.Walker;
import org.broadinstitute.sting.utils.StingException;
import org.broadinstitute.sting.utils.xReadLines;
import org.broadinstitute.sting.utils.cmdLine.*;
import java.io.FileNotFoundException;
import java.io.File;
import java.util.List;
import java.util.ArrayList;
import net.sf.samtools.SAMFileReader;
/**
*
* User: aaron
* Date: May 8, 2009
* Time: 10:50:58 AM
*
* The Broad Institute
* SOFTWARE COPYRIGHT NOTICE AGREEMENT
* This software and its documentation are copyright 2009 by the
* Broad Institute/Massachusetts Institute of Technology. All rights are reserved.
*
* This software is supplied without any warranty or guaranteed support whatsoever. Neither
* the Broad Institute nor MIT can be responsible for its use, misuse, or functionality.
*
*/
/**
* @author aaron
* @version 1.0
* @date May 8, 2009
*
* Class CommandLineGATK
*
* We run command line GATK programs using this class. It gets the command line args, parses them, and hands the
* gatk all the parsed out information. Pretty much anything dealing with the underlying system should go here,
* the gatk engine should deal with any data related information.
*/
public class CommandLineGATK extends CommandLineProgram {
@Argument(fullName = "analysis_type", shortName = "T", doc = "Type of analysis to run")
public String analysisName = null;
@ArgumentCollection // our argument collection, the collection of command line args we accept
public GATKArgumentCollection argCollection = new GATKArgumentCollection();
/** our genome analysis engine */
GenomeAnalysisEngine GATKEngine = new GenomeAnalysisEngine();
/** Required main method implementation. */
public static void main(String[] argv) {
try {
CommandLineGATK instance = new CommandLineGATK();
start(instance, argv);
} catch (Exception e) {
exitSystemWithError(e);
}
}
/**
* this is the function that the inheriting class can expect to have called
* when the command line system has initialized.
*
* @return the return code to exit the program with
*/
protected int execute() {
Walker,?> mWalker = GATKEngine.getWalkerByName(analysisName);
loadArgumentsIntoObject(argCollection);
loadArgumentsIntoObject(mWalker);
processArguments(argCollection);
this.argCollection.analysisName = this.analysisName;
GATKEngine.execute(argCollection, mWalker);
return 0;
}
/**
* Get pleasing info about the GATK.
* @return
*/
@Override
protected List getApplicationHeader() {
List header = new ArrayList();
header.add("The Genome Analysis Toolkit (GATK)");
header.add("Copyright (c) 2009 The Broad Institute");
header.add("Please view our documentation at http://www.broadinstitute.org/gsa/wiki");
header.add("For support, email gsadevelopers@broadinstitute.org");
header.add("");
return header;
}
/**
* GATK can add arguments dynamically based on analysis type.
*
* @return true
*/
@Override
protected boolean canAddArgumentsDynamically() {
return true;
}
/**
* GATK provides the walker as an argument source. As a side-effect, initializes the walker variable.
*
* @return List of walkers to load dynamically.
*/
@Override
protected Class[] getArgumentSources() {
// No walker info? No plugins.
if (analysisName == null) return new Class[] {};
return new Class[] { GATKEngine.getWalkerByName(analysisName).getClass() };
}
@Override
protected String getArgumentSourceName(Class argumentSource) {
return WalkerManager.getWalkerName((Class) argumentSource);
}
public GATKArgumentCollection getArgCollection() {
return argCollection;
}
public void setArgCollection(GATKArgumentCollection argCollection) {
this.argCollection = argCollection;
}
/**
* Get a custom factory for instantiating specialty GATK arguments.
* @return An instance of the command-line argument of the specified type.
*/
@Override
protected ArgumentFactory getCustomArgumentFactory() {
return new ArgumentFactory() {
public Object createArgument( Class type, List repr ) {
if (type == SAMFileReader.class && repr.size() == 1) {
SAMFileReader samFileReader = new SAMFileReader(new File(repr.get(0)),true);
samFileReader.setValidationStringency(argCollection.strictnessLevel);
return samFileReader;
}
return null;
}
};
}
/**
* Preprocess the arguments before submitting them to the GATK engine.
* @param argCollection Collection of arguments to preprocess.
*/
private void processArguments( GATKArgumentCollection argCollection ) {
argCollection.samFiles = unpackReads( argCollection.samFiles );
}
/**
* Unpack the files to be processed, given a list of files. That list of files can
* itself contain lists of other files to be read.
* @param inputFiles
* @return
*/
private List unpackReads( List inputFiles ) {
List unpackedReads = new ArrayList();
for( File inputFile: inputFiles ) {
if( inputFile.getName().endsWith(".list") ) {
try {
for( String fileName : new xReadLines(inputFile) )
unpackedReads.add( new File(fileName) );
}
catch( FileNotFoundException ex ) {
throw new StingException("Unable to find file while unpacking reads", ex);
}
}
else
unpackedReads.add( inputFile );
}
return unpackedReads;
}
}