/* * Copyright (c) 2010. * * Permission is hereby granted, free of charge, to any person * obtaining a copy of this software and associated documentation * files (the "Software"), to deal in the Software without * restriction, including without limitation the rights to use, * copy, modify, merge, publish, distribute, sublicense, and/or sell * copies of the Software, and to permit persons to whom the * Software is furnished to do so, subject to the following * conditions: * * The above copyright notice and this permission notice shall be * included in all copies or substantial portions of the Software. * * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, * EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES * OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND * NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT * HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, * WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING * FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR * THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ package org.broadinstitute.sting.oneoffprojects.walkers; import org.broad.tribble.util.variantcontext.VariantContext; import org.broad.tribble.vcf.*; import org.broadinstitute.sting.commandline.Output; import org.broadinstitute.sting.gatk.contexts.AlignmentContext; import org.broadinstitute.sting.gatk.contexts.ReferenceContext; import org.broadinstitute.sting.gatk.refdata.RefMetaDataTracker; import org.broadinstitute.sting.gatk.walkers.Reference; import org.broadinstitute.sting.gatk.walkers.Requires; import org.broadinstitute.sting.gatk.walkers.RodWalker; import org.broadinstitute.sting.gatk.walkers.Window; import org.broadinstitute.sting.utils.vcf.VCFUtils; import java.util.*; /** * Assesses calls missing from the BI set that are made by all other centers. * Use -B:broad,vcf and -B:1kg,vcf */ @Reference(window=@Window(start=-50,stop=50)) @Requires(value={}) public class AssessMissingBroadCalls extends RodWalker { private static final String status_key = "BI_STATUS"; private static final String qual_key = "BI_QUAL"; @Output(doc="File to which variants should be written",required=true) protected VCFWriter writer = null; public void initialize() { final ArrayList inputNames = new ArrayList(); inputNames.add("1kg"); // setup the header fields Set hInfo = new HashSet(); hInfo.addAll(VCFUtils.getHeaderFields(getToolkit(), inputNames)); writer.writeHeader(new VCFHeader(hInfo, new HashSet())); } public Integer map(RefMetaDataTracker tracker, ReferenceContext ref, AlignmentContext context) { if ( tracker == null ) // RodWalkers can make funky map calls return 0; List kgRods = tracker.getReferenceMetaData("1kg"); // ignore places where we don't have a variant if ( kgRods.size() == 0 ) return 0; VariantContext vc = (VariantContext)kgRods.get(0); Map attrs = new HashMap(vc.getAttributes()); List biRods = tracker.getReferenceMetaData("broad"); if ( biRods.size() == 0 ) attrs.put(status_key, "NotCalled"); else { VariantContext BIvc = (VariantContext)biRods.get(0); // skip the site if we called it if ( !BIvc.isFiltered() ) return 0; attrs.put(qual_key, BIvc.getPhredScaledQual()); Set filters = BIvc.getFilters(); StringBuilder sb = new StringBuilder(); for ( String filter : filters ) { if ( sb.length() != 0 ) sb.append("-"); sb.append(filter); } attrs.put(status_key, sb.toString()); } vc = VariantContext.modifyAttributes(vc, attrs); writer.add(vc, ref.getBase()); return 1; } public Integer reduceInit() { return 0; } public Integer reduce(Integer counter, Integer sum) { return counter + sum; } public void onTraversalDone(Integer sum) {} }