Commit Graph

105 Commits (ff26f2bf688048bbd6e2b9ffcf31cedce4fa99dd)

Author SHA1 Message Date
Christopher Hartl 2c1b14d35e Mostly small changes to my own scala scripts: .vcf.gz compatibility for output files, smarter beagle generation, simple script to scatter-gather combine variants. Whole genome indel calling now uses the gold standard indel set. 2012-02-22 17:20:04 -05:00
Christopher Hartl 974c2499cc Bugfixed to script. 2012-02-02 12:55:54 -05:00
Christopher Hartl 27ea6426a4 Small script to chunk up a VCF into equal-sized chunks 2012-02-02 12:29:03 -05:00
Christopher Hartl 0c562756eb Add a memory limit so this thing doesn't get killed on the farm 2012-02-02 10:30:09 -05:00
Christopher Hartl 45bf2562cc . 2012-02-02 09:11:17 -05:00
Christopher Hartl f8c5406084 Add the ability to extract samples 2012-02-02 09:06:39 -05:00
Christopher Hartl b567ed8793 Use the right reference path :( 2012-02-01 12:35:18 -05:00
Christopher Hartl 87a63d54d6 fix the script! 2012-02-01 12:05:29 -05:00
Christopher Hartl 810996cfca Introducing: VariantsToPed, the world's most annoying walker! And also a busted QScript to run it that I need Khalid's help debugging ( frownie face ). Note that VariantsToPed and PlinkSeq generate the same binary file (up to strand flips...thanks PlinkSeq), so I know it's working properly. Hooray! 2012-02-01 10:39:03 -05:00
Mauricio Carneiro 052a4bdb9c Turning off PHONE HOME option in the MDCP
* MDCP is for internal use and there is no need to report to the Amazon cloud.
   * Reporting to ASW_S3 is not allowing jobs to finish, this is probably a bug.
2012-01-27 11:13:30 -05:00
Mauricio Carneiro 97499529c7 another small bug with the file extension. 2012-01-24 16:14:35 -05:00
Mauricio Carneiro 945cf03889 IntelliJ ate my import! 2012-01-23 21:46:45 -05:00
Mauricio Carneiro 2bb9525e7f Don't set base qualities if fastQ is provided
* Pacbio Processing pipeline now works with the new fastQ files outputted by the Pacbio instrument
2012-01-23 17:57:29 -05:00
Khalid Shakir c18beadbdb Device files like /dev/null are now tracked as special by Queue and are not used to generate .out file paths, scattered into a temporary directory, gathered, deleted, etc.
Attempted workaround for xdr_resourceInfoReq unsatisfied link during loading of libbat.so.
2012-01-23 16:17:04 -05:00
Ryan Poplin 75f87db468 Replacing Mills file with new gold standard indel set in the resource bundle for release with v1.5 2012-01-17 15:02:45 -05:00
Mauricio Carneiro 5bf960deb8 adding dbsnp to indel VQSR 2012-01-10 12:38:49 -05:00
Mauricio Carneiro 6f2abd76df Updating the MDCP with the new indel gold standard from Ryan. 2012-01-09 15:31:18 -05:00
Khalid Shakir 5793625592 No more "Q-<pid>@<host>". Generated log file names now use the first output + ".out" (ex. my.vcf.out) or the name of the first QScript plus the order the function was added (ex. MyScript-1.out). The same function added twice with the same outputs will now have the same default logs, meaning the 2nd instance of the function won't be added to the graph twice.
QScript accessor to QSettings to specify a default runName and other default function settings.
Because log files are no longer pseudo-random their presense can be used to tell if a job without other file outputs is "done". For now still using the log's .done file in addition to original outputs.
Gathered log files concatenate all log files together into the stdout.
InProcessFunctions now have PrintStreams for stdout and stderr.
Updated ivy to use commons-io 2.1 for copying logs to the stdout PrintStream. Removed snakeyaml.
During graph tracking of outputs the Index files, and now BAM MD5s, are tracked with the gathering of the original file.
In Queue generated wrappers for the GATK the Index and MD5s used for tracking are switched to private scope.
Added more detailed output when running with -l DEBUG.
Simplified graphviz visualization for additional debugging.
Switched usage of the scala class 'List' to the trait 'Seq' (think java.util.ArrayList vs. using the interface java.util.List)
Minor cleanup to build including sending ant gsalib to R's default libloc.
2012-01-08 12:11:55 -05:00
Mauricio Carneiro f6a18aea63 Updated MDCP with INDEL best practices
* chose 90.0 indel cut target for most datasets (this is arbitrary).
2012-01-06 17:21:59 -05:00
Khalid Shakir 7486696c07 When using bam list mode in HSP deriving VCF name from bam list instead of requiring an additional parameter.
Creating a single temporary directory per ant test run instead of a putting temp files across all runs in the same directory.
Updated various tests for above items and other small fixes.
2011-12-16 18:09:25 -05:00
Mauricio Carneiro 663184ee9d Added test mode to PPP
* in test mode, no @PG tags are output to the final bam file
* updated pipeline test to use -test mode.
* MD5s updated accordingly
2011-12-12 18:29:06 -05:00
Mauricio Carneiro a3c3d72313 Added test mode to DPP
* in test mode, no @PG tags are output to the final bam file
* updated pipeline test to use -test mode.
* MD5s are now dependent on BWA version
2011-12-12 18:29:06 -05:00
Mauricio Carneiro cca8a18608 PPP pipeline test
* added a pipeline test to the Pacbio Processing Pipeline.
* updated exampleBAM with more complete RG information so we can use it in a wider variety of pipeline tests
* added exampleDBSNP.vcf file with only chromosome 1 in the range of the exampleFASTA.fasta reference for pipeline tests
2011-12-11 17:32:21 -05:00
Mauricio Carneiro 21ac3b59d7 Merged bug fix from Stable into Unstable 2011-12-09 16:51:46 -05:00
Mauricio Carneiro 13905c00b3 Updating PacbioProcessingPipeline to new Queue standards 2011-12-09 16:51:02 -05:00
David Roazen d014c7faf9 Queue now properly escapes all shell arguments in generated shell scripts
This has implications for both Qscript authors and CommandLineFunction authors.

Qscript authors:
You no longer need to (and in fact must not) manually escape String values to
avoid interpretation by the shell when setting up Walker parameters. Queue will
safely escape all of your Strings for you so that they'll be interpreted literally. Eg.,

Old way:
filterSNPs.filterExpression = List("\"QD<2.0\"", "\"MQ<40.0\"", "\"HaplotypeScore>13.0\"")

New way:
filterSNPs.filterExpression = List("QD<2.0", "MQ<40.0", "HaplotypeScore>13.0")

CommandLineFunction authors:
If you're writing a one-off CommandLineFunction in a Qscript and don't really
care about quoting issues, just keep doing things the direct, simple way:

def commandLine = "cat %s | grep -v \"#\" > %s".format(files, out)

If you're writing a CommandLineFunction that will become part of Queue and
will be used by other QScripts, however, it's advisable to do things the
newer, safer way, ie.:

When you construct your commandLine, you should do so ONLY using the API methods
required(), optional(), conditional(), and repeat(). These will manage quoting
and whitespace separation for you, so you shouldn't insert quotes/extraneous
whitespace in your Strings. By default you get both (quoting and whitespace
separation), but you can disable either of these via parameters. Eg.,

override def commandLine = super.commandLine +
                           required("eff") +
                           conditional(verbose, "-v") +
                           optional("-c", config) +
                           required("-i", "vcf") +
                           required("-o", "vcf") +
                           required(genomeVersion) +
                           required(inVcf) +
                           required(">", escape=false) +  // This will be shell-interpreted
                           required(outVcf)

I've ported the Picard/Samtools/SnpEff CommandLineFunction classes to the new
system, so you'll get free shell escaping when you use those in Qscripts just
like with walkers.
2011-12-01 18:13:44 -05:00
Mauricio Carneiro dbd8c25787 No more R resources in the DPP
updating the DPP to conform with Analyze Covariates changes.
2011-10-28 16:57:01 -04:00
Mauricio Carneiro 86305a5dcf Adjusting the memory limits of the MDCP
Indel caller needs more than 3G for large datasets.
2011-10-21 17:41:52 -04:00
Mauricio Carneiro c9d8b22092 Added BWASW support to the pipeline
Data Processing Pipeline can now use BWASW for realigning the reads. Useful for Ion Torrent data.
2011-10-20 18:36:28 -04:00
Mauricio Carneiro 093cd95c5d Merged bug fix from Stable into Unstable 2011-10-20 17:03:22 -04:00
Mauricio Carneiro d7367c152a Fixing 'revert' when not realigning
RevertSam was reverting the alignment information and that was screwing up the pipeline if you didn't want to run it with BWA. Fixed.
2011-10-20 17:01:54 -04:00
Mauricio Carneiro ed402588cc Adding the "gold standard NA12878" target 2011-10-20 16:19:13 -04:00
Mauricio Carneiro 0939d16a8d String not empty bug
Apparently var X: String = _ is not the same as var X: String = "".  :(
2011-10-13 13:22:05 -04:00
Mauricio Carneiro 66b5646f95 Adding hidden options to the DPP
controlling the default platform parameter to Count Covariates and the number of scatter gather jobs to generate are now available under hidden parameters
2011-10-11 13:56:00 -04:00
Mark DePristo a91509e7dd Shouldn't be public 2011-10-05 15:22:57 -07:00
Mauricio Carneiro d3cc25454c Updating the MDCP 2011-09-22 11:27:40 -04:00
Mauricio Carneiro 623c49765d NO BAQ ON EXOMES!
says the boss.
2011-09-22 11:13:40 -04:00
Ryan Poplin 5d0f284305 Fixing exome specific arguments to the VQSR in the methods development calling pipeline 2011-09-21 20:26:28 -04:00
Mauricio Carneiro 758ecf2d43 Bringing latest updates of ReduceReads to the master repository 2011-09-20 16:35:09 -04:00
Mauricio Carneiro 08ffb18b96 Renaming datasets in the MDCP
Making dataset names and files generated by the MDCP more uniform.
2011-09-20 11:02:51 -04:00
Eric Banks ba150570f3 Updating to use new rod system syntax plus name change for CountRODs 2011-09-19 13:30:32 -04:00
Eric Banks 85626e7a5d We no longer want people to use the August 2010 Dindel calls for indel realignment but instead Guillermo's new whole genome bi-allelic indel calls; updating the bundle accordingly. Also, there was some confusion by the 1000G data processing folks as to exactly what these indel files are, so I've renamed them so that it's clear. Wiki updated too. 2011-09-19 12:24:05 -04:00
Khalid Shakir 33967a4e0c Fixed issue reported by chartl where cloned functions lost tags on @Inputs.
Updated ExampleUnifiedGenotyper.scala with new syntax.
2011-09-16 12:46:07 -04:00
Ryan Poplin 981b78ea50 Changing the VQSR command line syntax back to the parsed tags approach. This cleans up the code and makes sure we won't be parsing the same rod file multiple times. I've tried to update the appropriate qscripts. 2011-09-12 12:17:43 -04:00
Mauricio Carneiro 7f9000382e Making indel calls default in the MDCP
You can turn off indel calling by using -noIndels.
2011-09-09 14:09:26 -04:00
Mauricio Carneiro ee9d599558 Just cleaning up
clean up old commented code from tha data processing pipeline.
2011-09-07 13:32:40 -04:00
Mauricio Carneiro 28d782b4c7 Allowing multiple dnsnp and indel files in the DPP 2011-09-02 13:38:47 -04:00
Mauricio Carneiro ad4ea0b80b Merged bug fix from Stable into Unstable 2011-09-01 18:14:45 -04:00
Mauricio Carneiro e253f6f05d Fixing typo in DPP
platform and library were exchanged when rebuilding the read group information
2011-09-01 18:13:52 -04:00
Mauricio Carneiro d2a33beff7 Added WGS/WEX b37-decoy CEU trio datasets 2011-09-01 13:14:40 -04:00