Commit Graph

26 Commits (e48078b47670c7a0cd5c7a4b25eb4e88929944a7)

Author SHA1 Message Date
kiran e48078b476 Updated to reflect change to BasecallingReadModel constructor.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@760 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-20 15:43:26 +00:00
kiran 505f588768 Forgot to say that the mate is unmapped too. This is necessary to prevent SAM-JDK from yelling at me about an invalid SAM file.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@759 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-20 15:38:51 +00:00
kiran 6c5fbb988b Now basecalls an entire read (both ends of the pair, barcode... everything) at once. After, RawRead and FourProbRead can be asked to return a specified subset (corresponding to the ranges specified for each end of the read.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@754 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-20 00:09:20 +00:00
kiran e293d65ede Refactored to allow the user to specify the range of cycles they wish to call. Simply specify a single range (i.e. '0-75') or two ranges ('0-75,76-151'). This allows single and paired-end read processing to coexist happily. Also implements annotation of an aligned bam file (which should hopefully fit in under two gigs now, but I'm waiting on a bug fix or a clarification from the Picard team.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@753 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-20 00:07:24 +00:00
kiran 08c9f4d86b Renamed to BasecallingTrainer.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@752 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-20 00:03:46 +00:00
kiran 7c615c8fb0 Some changes to the system for annotating a pre-aligned bam file. Doesn't fit within 2gigs.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@746 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-18 17:42:08 +00:00
kiran 28bf7ec8ad Aesthetic cleanup.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@735 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-17 04:09:23 +00:00
kiran a0464633fd Whoops. Changed denominator from reads to bases.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@734 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-17 03:42:25 +00:00
kiran 5d60efc498 Factored out some simple stats accumulation.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@733 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-17 03:37:57 +00:00
kiran 6f1559bd77 Cleaned up a bit. Added some documentation.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@728 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-15 21:22:24 +00:00
kiran dae77bf14a Fixed a typo in a comment.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@723 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-15 20:07:31 +00:00
kiran bfc40f54f0 Nicer output when training off of perfect reads. Not that that works yet...
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@722 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-15 20:07:08 +00:00
kiran 36db44620b Improved output. Can optionally limit the number reads actually called.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@720 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-15 00:07:57 +00:00
kiran 5858f20902 Documentation.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@712 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-14 18:58:43 +00:00
kiran 3761c0900b Added Bustard vs. Four-prob percent bases consistent output.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@710 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-14 18:01:41 +00:00
kiran 959cf09d4b Removed some debugging print statements.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@707 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-14 17:12:42 +00:00
kiran 2f42a643a8 A new, much simpler (and now, complete) driver program for four-base probs. Serves as a model for anyone who wants to write their own driver program that trains and calls with data from a different source than the raw Illumina data.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@706 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-14 16:58:22 +00:00
kiran 5824dea0c1 Trains and calls a read at a time rather than a base at a time (which, given it's name, it should have done in the first place)
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@705 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-14 16:57:00 +00:00
kiran e4770885fd The four-probs for all bases in a single read. Some utility functions for generating the primary and secondary base strings, as well as generating the SQ tag byte array in a manner that's consistent with the Bustard base calls (meaning the primary Bustard call and the secondary Four-Prob call are not permitted to be the same).
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@704 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-14 16:55:49 +00:00
kiran fdd123fe16 A parser the raw Illumina data. Allows one to arbitrarily jump from one tile to another.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@703 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-14 16:53:07 +00:00
kiran 6d98234555 Holds raw intensities, sequence, and quality scores.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@701 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-14 16:52:03 +00:00
kiran 241de0b235 A class that implements multiple training strategies and presents the training data in a common form.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@700 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-14 16:51:29 +00:00
depristo 5b47c5ab6c fixing kiran's busted build
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@686 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-12 21:29:04 +00:00
kiran 4f2c8bf0a3 Fixed an import statement that broke when all the files were moved to this directory.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@685 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-12 20:43:16 +00:00
kiran cedc4c9ccb Refactored into oblivion.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@684 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-12 20:33:07 +00:00
kiran 688358190c Moved secondary base stuff out of playground for the purpose of making it a core utility. Modified package names and imports such that things would build properly.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@680 348d0f76-0448-11de-a6fe-93d51630548a
2009-05-12 20:24:18 +00:00