Commit Graph

9737 Commits (7fbca7013e7b9da220396d9bcaec209efdf67cb7)

Author SHA1 Message Date
Mark DePristo 7fbca7013e Don't add missing value binding from field to Genotype object in VCF3Codec 2012-06-14 16:42:28 -04:00
Mark DePristo cfd1e50068 Minor updates to test code 2012-06-14 16:42:28 -04:00
Mark DePristo 54817f8d16 VCFHeaderUnitTest needed to be updated to reflect that we are doing VCF4.1 not VCF4.0 2012-06-14 16:42:28 -04:00
Mark DePristo d74c46bbcb Convenient python script to update md5s in one go across all files
-- integration tests now write out mismatches file, which this script reads in, walks the file system to find files with these md5s, and updates them in the files.
-- Should be run like: python private/python/updateMD5s.py integrationtests/md5mismatches.txt public/java/test
-- Note that before using this code you should really commit your code for safety
-- Note also that you ***MUST*** review the differences before running this code.  It's not ok to just update md5s willy-nilly
-- Doc from the code:

    Update the MD5s across multiple files.

    The first argument is the database of MD5 updates produced automatically by the
    integrationtests, which lives in integrationtest/md5mismatches.txt.

    The second (and more) argument(s) is the path to the source directory containing the java / scala
    code with md5s to update.  This program crawls that directory looking for .java
    and .scala files to update, id's those with MD5s in the md5mismatches.txt files and
    updates these files.

    It is highly recommended that you commit your code before updating the MD5s to ensure
    that if things go wrong with this script that you don't lose any work.
2012-06-14 16:42:27 -04:00
Mark DePristo 982192e2e4 MD5DB for integrationtest management now writes out a md5mismatches files for clean analysis
-- This file is in integrationtests/md5mismatches.txt, and looks like:

expected        observed        test
7fd0d0c2d1af3b16378339c181e40611        2339d841d3c3c7233ebba9a6ace895fd        test BeagleOutputToVCF
43865f3f0d975ee2c5912b31393842f8        1b9c4734274edd3142a05033e520beac        testBeagleChangesSitesToRef
daead9bfab1a5df72c5e3a239366118e        27be14f9fc951c4e714b4540b045c2df        testDiffObjects:master=/local/dev/depristo/itest/public/testdata/diffTestMaster.vcf,test=/local/dev/depristo/itest/public/testdata/diffTestTest.vcf,md5=daead9bfab1a5df72c5e3a239366118e

-- Associated cleanup with making md5db an instantiated object, rather than a bunch of static methods
2012-06-14 16:42:27 -04:00
Mark DePristo aef4c5eec1 BCFvsVCF now tests subsetting of XCF to sites 2012-06-14 16:42:27 -04:00
Mark DePristo 249d5e5533 Better tests for Genotype parsing 2012-06-14 16:42:27 -04:00
Mark DePristo 4a4d3cde3d UnitTests for decodeIntArray method 2012-06-14 16:42:27 -04:00
Mark DePristo 5b8bd81991 An option to not actually write out the results of select variants
-- Useful for performance testing of the SV operations themselves.
2012-06-14 16:42:26 -04:00
Mark DePristo 6f7a01e00d Bugfix for BCF2 reader / writer for > 0x0FFF samples :-)
-- Should be 0x00FFFFFF in the mask
2012-06-14 16:42:26 -04:00
Mark DePristo 1d4eb46606 Efficient reading of genotype fields v1
-- decodeIntArray in BCF2 decoder allows us to more efficiently read ints and int[] from stream directly into Genotype object
-- Code cleanup / contracts added were appropriate
-- V2 will have a yet more optimized path...
2012-06-14 16:42:26 -04:00
Mark DePristo 3a70f8216b Updated BCF vs VCF performance logs 2012-06-14 16:42:26 -04:00
Mark DePristo 96dbd8df63 Fix a nasty script bug in Queue
-- If you are using user-defined configurations (configureJobFeatures) and you didn't overwride the analysisName of your jobs, and there were other jobs using the same name, then you got very strange errors at the end of your script.  For example, in my script I was using SelectVariants to prepare VCF files, and SelectVariants to generate a useful performance table.  Since I forgot to make a special analysisName for my table commands, the generic SV commands were being included in the analysis group, and these were throwing an error since the special features added for the table weren't added to those SV commands
2012-06-14 16:42:26 -04:00
Mark DePristo 37b8d70321 Hidden option to SelectVariants to force the genotypes information to be decoded by computing AC 2012-06-14 16:42:25 -04:00
Mark DePristo 17fbd103d0 Smarter infrastructure to decode genotypes in BCF
-- Eliminated the large intermediate map from field name to list of list<Integer> values needed to create genotypes without the GenotypeBuilder.  The new code is cleaner and simply fills in an array of GenotypeBuilders as it moves through the column layout in BCF2
-- Now we create once decoders specialized for each GT field (GT, AD, etc) that can be optimized for putting data into the GenotypeBuilder.  In a subsequent commit these will actually use lower level BCF2 decoders to create the low-level ints and int[], avoiding the intermediate List<Integer> form
-- Reduced the amount of data further to be computed in the DiffEngine.  The DiffEngine algorithm needs to be rethought to be efficient...
2012-06-14 16:42:25 -04:00
Mark DePristo 889e3c4583 Code cleanup before major refactor 2012-06-14 16:42:25 -04:00
Mark DePristo cebd37609c Finalizing new Genotype object and associated routines
-- Builder now provides a depreciated log10pError function to make a new GQ value
-- Genotype is an abstract class, with most of the associated functions implemented here and not in the derived Fast and Slow versions
-- Lots of contracts
-- Bugfixes throughout
2012-06-14 16:42:25 -04:00
Mark DePristo 8b0a629a31 Terrible bugfix
-- The way I was handling the contig offset ordering wasn't correct.  Now the contigs are always indexed in the order in which their corresponding populate() functions are called, so that the order of the contigs is given by the order in which they are in the file, or in our refDict.  It has nothing to do with the contig index itself.
-- SelectVariants no longers prints all samples to the screen if you aren't selecting any explicitly
2012-06-14 16:42:24 -04:00
Mark DePristo d37a8a0bc8 Efficient Genotype object Intermediate commit
-- Created a new Genotype interface with a more limited set of operations
-- Old genotype object is now SlowGenotype.  New genotype object is FastGenotype.  They can be used interchangable
-- There's no way to create Genotypes directly any longer.  You have to use GenotypeBuilder just like VariantContextBuilder
-- Modified lots and lots of code to use GenotypeBuilder
-- Added a temporary hidden argument to engine to use FastGenotype by default.  Current default is SlowGenotype
-- Lots of bug fixes to BCF2 codec and encoder.
-- Feature additions
  -- Now properly handles BCF2 -> BCF2 without decoding or encoding from scratch the BCF2 genotype bytes
  -- Cleaned up semantics of subContextFromSamples.  There's one function that either rederives or not the alleles from the subsetted genotypes

-- MASSIVE BUGFIX in SelectVariants.  The code has been decoding genotypes always, even if you were not subsetting down samples.  Fixed!
2012-06-14 16:42:24 -04:00
Mark DePristo a648b5e65e First step towards an efficient Genotype object
-- Created new clean FastGenotype and GenotypeBuilder classes with contracts to enforce expected behavior and correctness.  Tested utility of this approach by rewritting -- and then commenting out -- a path in BCF2Codec that could use this new code.  Much cleaner interface now, but not yet hooked up to anything
-- Disabled SHADOW_BCF generation and generating contigs in the output VCFs automatically to ensure that the current code bases integration tests, before switching the code to new Genotype class
-- Code cleanup.  Moved "AD" to VCFConstants under GENOTYPE_ALLELIC_DEPTHS.  Uses in code replaced with constant
2012-06-14 16:42:23 -04:00
Mark DePristo ff9ac4b5f8 BCF2 genotype decoding is now lazy
-- Refactored BCF2Codec into a LazyGenotypesDecoder object that provides on-demand genotype decoding of BCF2 data blocks a la VCFCodec.
-- VCFHeader has getters for sampleNamesInOrder and sampleNameToOffset instead of protected variables directly accessed by vcfcodec
2012-06-14 16:42:23 -04:00
Mark DePristo 9eb83a0771 Enable adding contigs to VariantContextWriters on output 2012-06-14 16:42:23 -04:00
Mark DePristo 8fc1a26ac7 Fixed comparison of VCFHeader as the set.equals() isn't working as expected 2012-06-14 16:42:22 -04:00
Mark DePristo b0ea14ef0f VCFHeader getMetaData returns 4.1 version not 4.0 2012-06-14 16:42:22 -04:00
Mark DePristo 5fda16bea9 Enable shadow BCF2 2012-06-14 16:42:22 -04:00
Mauricio Carneiro 7d12429917 First step towards indel qualities in RR
Let the BI's and BD's pass through the reduce reads machinery
2012-06-14 15:37:39 -04:00
Mauricio Carneiro e68038c5d8 Refactor post-processing downsampling using David's generic downsampler interface 2012-06-14 15:37:32 -04:00
Eric Banks 0398ae9695 I hate these disabled unit tests, #2 2012-06-14 15:19:27 -04:00
Eric Banks 676a57de7b I hate these disabled unit tests 2012-06-14 14:03:58 -04:00
Eric Banks 065fe9a7b9 Updating md5 for bug fixes 2012-06-14 13:03:51 -04:00
Eric Banks de5508fcea Bug fixes for cycle and context covariates 2012-06-14 13:01:14 -04:00
Eric Banks 5c3c6cbc40 Long -> long conversions in BQSR 2012-06-14 09:07:02 -04:00
Eric Banks 29a74908bb The next round of BQSR optimizations: no more Long[] array creation 2012-06-14 00:05:42 -04:00
Guillermo del Angel cd2074b1dc Merge branch 'master' of ssh://gsa4.broadinstitute.org/humgen/gsa-scr1/gsa-engineering/git/unstable 2012-06-13 20:59:30 -04:00
Guillermo del Angel 92669a0468 Second intermediate commit for indel pool caller - now works (more or less) in reference sample-free mode. Still needs a lot of cleanups/add more tests and not done w/refactoring quite yet 2012-06-13 20:59:17 -04:00
David Roazen 0550b27799 Make downsampler classes themselves generic (instead of just the Downsampler interface)
This is in response to a request from Mauricio to make it easier
to use the downsamplers with GATKSAMRecords (as opposed to SAMRecords)
without having to do any cumbersome typecasting. Sadly, Java
language limitations make this sort of solution the best choice.

Thanks to Khalid for his feedback on this issue.

Also:

-added a unit test to verify GATKSAMRecord support with no typecasting required

-added some unit tests for the FractionalDownsampler that Mauricio will/might be using

-moved classes from private to public to better sync up with my local development
branch for engine integration
2012-06-13 16:43:39 -04:00
Joel Thibault fc36177004 Accidentally removed the scattering from this test 2012-06-13 14:18:32 -04:00
Guillermo del Angel 67c0569f9c Merge branch 'master' of ssh://gsa4.broadinstitute.org/humgen/gsa-scr1/gsa-engineering/git/unstable 2012-06-13 11:50:00 -04:00
Guillermo del Angel 9a4c39357a Fixed bugs in previous commit 2012-06-13 11:49:20 -04:00
Eric Banks 81993b08e2 Don't put null entries into the key array 2012-06-13 11:43:44 -04:00
Roger Zurawicki bdf5945dcc Fixed bugs in DiagnoseTargets
DT would not report bad mates!
that has been fixed

Signed-off-by: Mauricio Carneiro <carneiro@broadinstitute.org>
2012-06-13 11:15:26 -04:00
Roger Zurawicki 538cdf9210 Created the FindCoveredIntervals
Moved some stuff in the DiagnoseTargets walker to the more general ThresHolder class
Minor tweaks
FindCoveredIntervals supports Gathering
FindCoveredIntervals outputs an interval list instead of GATKReport

Signed-off-by: Mauricio Carneiro <carneiro@broadinstitute.org>
2012-06-13 11:15:25 -04:00
Guillermo del Angel aee66ab157 Big UG refactoring and intermediate commit to support indels in pool caller (not done yet). Lots of code pulled out of long spaghetti-like functions and modularized to be easily shareable. Add functionality in ErrorModel to count indel matches/mismatches (but left part disabled as not to change integration tests in this commit), add computation of pool genotype likelihoods for indels (not fully working yet in more realistic cases, only working in artificial nice pools). Lot's of TBD's still but existing UG and pool SNP functionality should be intact 2012-06-13 11:14:44 -04:00
Eric Banks bb77aa88c3 Drat, forgot the unit tests again 2012-06-12 19:00:47 -04:00
Eric Banks bacd25d1a4 Merge branch 'master' of ssh://gsa2.broadinstitute.org/humgen/gsa-scr1/gsa-engineering/git/unstable 2012-06-12 16:15:36 -04:00
Eric Banks 37f56ce8fd A couple of minor updates to BQSR 2012-06-12 16:12:13 -04:00
Eric Banks 277493dd83 Yet more instances of Lists changed over to native arrays 2012-06-12 15:56:09 -04:00
Eric Banks 613badc835 Very minor optimizations for the context covariate 2012-06-12 15:47:32 -04:00
Joel Thibault c8cda209b5 Make intersection easy to change
Write each client's output to a different file
2012-06-12 15:46:34 -04:00
Eric Banks 0f79adb2aa Changing more Java Lists to native arrays in BQSR for performance optimization. 2012-06-12 15:41:01 -04:00