4d4ef5b42c
In the end, it's not worth rewriting TranscriptToInfo from scratch. I'm keeping the old one around for a bit so I can play with this new version which 1. doesn't store the records in memory so can be run in under 1Gb of memory, 2. actually emits all of the records (the original fails in some cases), and 3. is refactored to cut out ~20% of the code.
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4215 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-06 02:37:34 +00:00
0dd5a0990d
Now annotates sites marked as filtered out (this is important if sites are in a lower-quality tranche).
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4214 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-04 00:36:55 +00:00
e9af893bf4
Write headers that are VCF4.0 compliant
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4213 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-03 23:46:27 +00:00
ef7454a241
Minor improvements to indel genotyper:
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a) Ability to specify haplotype size from command line
b) Expand reference context window so we can form haplotypes for longer indel events.
c) small bug fix in temp output writer (to be removed once I can emit vcfs)
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4212 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-03 22:52:08 +00:00
7eeabe534a
QSample walker for 1KG -- measures aggregate quality of sequencing. Includes misc. improvements throughtout the code, including using the new Tribble GenotypeLikelihoods class for working with VCF GLs from the UG
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4211 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-03 18:21:43 +00:00
e3962c0d13
VR integration tests are longer but much more useful.
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4210 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-03 15:50:19 +00:00
da11efa1a2
Automatically write BAM file indices for coordinate-sorted BAMs.
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4209 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-03 14:10:44 +00:00
529eecd4dc
Added phasing sub-directory to keep walkers directory clean
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4208 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-03 13:38:46 +00:00
c0ce9ca8cc
Added phasing sub-directory to keep walkers directory clean
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4207 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-03 13:32:30 +00:00
60003aeaca
Bug fix in VariantRecalibrator. Only add sample names from the input rod bindings, not from all rod bindings.
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4206 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-03 13:31:49 +00:00
c119f64514
Added phasing sub-directory to keep walkers directory clean
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4205 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-03 13:24:18 +00:00
fc13191352
Added phasing sub-directory to keep walkers directory clean
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4204 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-03 13:23:24 +00:00
3c9597d45a
OnTraversalDone writes output to out now
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4203 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-03 12:55:03 +00:00
73d41bfa24
CountLoci nows writes out to a file for Queue status tracking. VariantAnnotatorEngine has a special group None that doesn't add any annotations; useful for those who are testing UG performance
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4202 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-03 12:52:33 +00:00
b59d62927e
Fix busted performance test (-outputBam has been deprecated in the BQ recalibrator in favor of -o)
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4201 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-03 12:51:53 +00:00
70bb480939
The battle is over. Picard is revved.
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4200 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-03 05:28:01 +00:00
fdaac4aa78
As the VCF guru, I'll take this one for Andrey. Someone has actually found a deletion at the beginning of the chromosome. Instead of failing with an ArrayIndexOutOfBoundsException, just don't try to print out the record. Our VCF writer doesn't really support this case (yet).
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4199 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-03 03:27:43 +00:00
c45ffcdaed
Changing documentation (temporarily) to warn people that -U is not supported.
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4198 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-03 03:18:07 +00:00
8a7f5aba4b
First more or less sort of functional framework for statistical Indel error caller. Current implementation computes Pr(read|haplotype) based on Dindel's error model. A simple walker that takes an existing vcf, generates haplotypes around calls and computes genotype likelihoods is used to test this as first example. No attempt yet to use prior information on indel AF, nor to use multi-sample caller abilities.
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4197 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-03 00:25:34 +00:00
a1cf3398a5
Added basic version of phasing evaluation: GenotypePhasingEvaluator
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4196 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-02 22:09:50 +00:00
fd5970fdd4
At chartl's superb suggestion, command line files are now all Files instead of old method of sometimes "has a File". Should be easier when reassigning them.
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No longer generating deprecated GATK arguments on the Queue extensions.
Emitting deprecation warnings to Queue compile to help debugging issues.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4195 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-02 21:30:48 +00:00
0bb05fb472
Bug fix in VariantRecalibrator. Only add sample names from the input rod bindings, not from all rod bindings.
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4194 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-02 21:12:09 +00:00
3a4844ebde
Additional partition types into DepthOfCoverage:
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- Sequencing Center
- Platform
- Sample by Center
- Sample by Platform
- Sample by Platform by Center <---- needed for analysis I'm doing
The fact that the latter three needed their own partition types, rather than being dictatable from the command line, combined with the new hierarchical traversal types, and new output formatting engine, suggest that DepthOfCoverageV3 is about ready to be retired in favor of a newer, sleeker version.
For now, this will do.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4193 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-02 19:30:03 +00:00
590bb50d16
Test for missing read group
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4192 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-02 14:22:13 +00:00
acd6bd2430
Experimental tool to annotates indels that are provided in a VCF file based on RefGene. Specifies gene, transcript, strand, type (Non-frameshift, frameshift, 5'-UTR, 3'-UTR, SpliceSiteDisruption, Intron, or Unknown).
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4191 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-01 23:30:28 +00:00
dc5f858d29
Replaced placeholder support for splitting by read group with read support (sorry everyone), and added relatively comprehensive unit tests to ensure that splitting by read group works.
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4190 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-01 22:24:50 +00:00
b28f63a948
Base recalibrator now uses -o and deprecates -outputBam
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4189 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-01 22:13:50 +00:00
33400074fa
Updated tribble BED parsing code to use the official UCSC spec, and updated tests to match expected results.
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4188 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-01 21:49:06 +00:00
924e16f4f0
More robust analysis tool
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4187 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-01 20:24:04 +00:00
ca503e5801
Queue scripts for recalibration and running nSample UG jobs pre and dynamic merging
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4186 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-01 20:23:37 +00:00
995cfe34fe
You can have an error so early that some engine fields are uninitialized. Commit protects RunReport from these errors
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4185 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-01 19:00:25 +00:00
a975db2c2e
Bug fix for the case of reads with no read bases!
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4184 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-01 16:58:54 +00:00
0c54bf4195
Better reporting and now with a special mode for listing exceptions
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4183 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-01 16:19:51 +00:00
cdad243645
updated version of the DPR. Now produces part of the tearsheet as well as good depth of coverage figures
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4182 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-01 15:38:58 +00:00
469bbaa240
Added more integration tests for the variant quality score recalibrator
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4181 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-01 15:31:24 +00:00
fc5caa98a5
Improved reporting now with metrics by day/week/etc.
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4180 348d0f76-0448-11de-a6fe-93d51630548a
2010-09-01 02:43:13 +00:00
8c4009ee18
Oops, don't enable reporting in integration tests
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4179 348d0f76-0448-11de-a6fe-93d51630548a
2010-08-31 22:56:18 +00:00
5b94c926c8
More precise language.
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4178 348d0f76-0448-11de-a6fe-93d51630548a
2010-08-31 21:44:22 +00:00
96040726ac
Better exception text for the common error of providing only dbsnp but giving dbsnp sites zero clustering weight.
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4177 348d0f76-0448-11de-a6fe-93d51630548a
2010-08-31 21:36:43 +00:00
8683087756
Suppl. tools for working with and displaying GATK run reports
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4176 348d0f76-0448-11de-a6fe-93d51630548a
2010-08-31 20:32:22 +00:00
32c6b48106
Proper memory metrics in the file. Please use -et if at all possible
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4175 348d0f76-0448-11de-a6fe-93d51630548a
2010-08-31 20:30:09 +00:00
63c7cbd89b
Forgot to commit this long ago, change so the tables are correctly propagated
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4174 348d0f76-0448-11de-a6fe-93d51630548a
2010-08-31 19:06:52 +00:00
db4ff7317f
allowing empty RMD files (we need to not validate their sequence dictionaries against the reference in this case)
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4173 348d0f76-0448-11de-a6fe-93d51630548a
2010-08-31 17:45:33 +00:00
3d6c4fc55f
Removing the obsolete --hapmap and --hapmap_chip options
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4172 348d0f76-0448-11de-a6fe-93d51630548a
2010-08-31 16:57:05 +00:00
b33873206a
GATKRunReport now has an ID (random 32 char string) that uniquely identifies the JOB run and can be used to find a run in the run repository
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4171 348d0f76-0448-11de-a6fe-93d51630548a
2010-08-31 16:18:57 +00:00
5e710050d6
minor change, bamFiles comes from the input list, not the script
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4170 348d0f76-0448-11de-a6fe-93d51630548a
2010-08-31 16:03:35 +00:00
1a14dbee1e
Adding in .bam indexing; commit for Khalid
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4169 348d0f76-0448-11de-a6fe-93d51630548a
2010-08-31 15:21:41 +00:00
3c956110f3
Fixing up the VCFWriter storage code: instead of assuming all samples are coming from the input bam file (they're not), just use the original VCF header for writing the temporary thread files. Now parallelization in e.g. the Genomic Annotator works.
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4168 348d0f76-0448-11de-a6fe-93d51630548a
2010-08-31 02:16:07 +00:00
69d92fab4f
adding the ability to get iterators from Tribble without having an index, and updating the Tabix code to the latest Samtools SVN version (this still doesn't fix the outstanding tabix bugs, waiting for Heng on that).
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4167 348d0f76-0448-11de-a6fe-93d51630548a
2010-08-30 21:49:23 +00:00
50f7f18cbd
Changed ReadBackedPhasing default PQ threshold to 10
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git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4166 348d0f76-0448-11de-a6fe-93d51630548a
2010-08-30 21:26:15 +00:00
ebanks
kiran
delangel
depristo
rpoplin
hanna
fromer
kshakir
chartl
corin
aaron