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Added a method to ReadUtils that converts reads of the form 10S20M10S to 40M (just unclips the soft-clips). 

Be careful when using this - if you're writing a bam file it will be potentially written out of order (since the previous alignment start was at the M, not the S).
This commit is contained in:
Christopher Hartl 2011-09-21 18:16:42 -04:00
parent faff6e4019
commit f9cdc119af
1 changed files with 45 additions and 0 deletions
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45
public/java/src/org/broadinstitute/sting/utils/sam/ReadUtils.java 100644 → 100755
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@ -681,6 +681,9 @@ public class ReadUtils {
@Ensures({"result >= read.getUnclippedStart()", "result <= read.getUnclippedEnd() || readIsEntirelyInsertion(read)"}) @Ensures({"result >= read.getUnclippedStart()", "result <= read.getUnclippedEnd() || readIsEntirelyInsertion(read)"})
public static int getRefCoordSoftUnclippedEnd(SAMRecord read) { public static int getRefCoordSoftUnclippedEnd(SAMRecord read) {
if ( read.getCigar().numCigarElements() == 1 && read.getCigar().getCigarElement(0).getOperator().equals(CigarOperator.INSERTION)) {
return read.getUnclippedEnd();
}
int stop = read.getUnclippedStart(); int stop = read.getUnclippedStart();
if (readIsEntirelyInsertion(read)) if (readIsEntirelyInsertion(read))
@ -787,5 +790,47 @@ public class ReadUtils {
return readBases; return readBases;
} }
public static SAMRecord unclipSoftClippedBases(SAMRecord rec) {
int newReadStart = rec.getAlignmentStart();
int newReadEnd = rec.getAlignmentEnd();
List<CigarElement> newCigarElements = new ArrayList<CigarElement>(rec.getCigar().getCigarElements().size());
int heldOver = -1;
boolean sSeen = false;
for ( CigarElement e : rec.getCigar().getCigarElements() ) {
if ( e.getOperator().equals(CigarOperator.S) ) {
newCigarElements.add(new CigarElement(e.getLength(),CigarOperator.M));
if ( sSeen ) {
newReadEnd += e.getLength();
sSeen = true;
} else {
newReadStart -= e.getLength();
}
} else {
newCigarElements.add(e);
}
}
// merge duplicate operators together
int idx = 0;
List<CigarElement> finalCigarElements = new ArrayList<CigarElement>(rec.getCigar().getCigarElements().size());
while ( idx < newCigarElements.size() -1 ) {
if ( newCigarElements.get(idx).getOperator().equals(newCigarElements.get(idx+1).getOperator()) ) {
int combSize = newCigarElements.get(idx).getLength();
int offset = 0;
while ( idx + offset < newCigarElements.size()-1 && newCigarElements.get(idx+offset).getOperator().equals(newCigarElements.get(idx+1+offset).getOperator()) ) {
combSize += newCigarElements.get(idx+offset+1).getLength();
offset++;
}
finalCigarElements.add(new CigarElement(combSize,newCigarElements.get(idx).getOperator()));
idx = idx + offset -1;
} else {
finalCigarElements.add(newCigarElements.get(idx));
}
idx++;
}
rec.setCigar(new Cigar(finalCigarElements));
rec.setAlignmentStart(newReadStart);
return rec;
}
} }