Walker that calculates the percentage of bases that are covered to at least 20x. Very useful! In oneoffs until someone else thinks it's as useful as I think it is ;)
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@5714 348d0f76-0448-11de-a6fe-93d51630548a
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package org.broadinstitute.sting.oneoffprojects.walkers;
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import org.broadinstitute.sting.commandline.Argument;
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import org.broadinstitute.sting.commandline.Output;
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import org.broadinstitute.sting.gatk.contexts.AlignmentContext;
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import org.broadinstitute.sting.gatk.contexts.ReferenceContext;
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import org.broadinstitute.sting.gatk.refdata.RefMetaDataTracker;
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import org.broadinstitute.sting.gatk.walkers.LocusWalker;
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import org.broadinstitute.sting.gatk.walkers.TreeReducible;
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import java.io.PrintStream;
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/**
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* Walks over the input data set, calculating the total number of covered loci for diagnostic purposes.
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* Simplest example of a locus walker.
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*/
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public class Percent20xCoverage extends LocusWalker<Integer, Long> implements TreeReducible<Long> {
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@Output(doc="Write count to this file instead of STDOUT")
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PrintStream out;
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@Argument(fullName="target_coverage", shortName="coverage", doc="Set the target coverage", required=false)
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private int targetCoverage = 20;
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private long totalLoci;
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private long totalCoverage;
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public void initialize () {
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totalLoci = 0;
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totalCoverage = 0;
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}
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public Integer map(RefMetaDataTracker tracker, ReferenceContext ref, AlignmentContext context) {
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totalLoci++;
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int coverage = context.getBasePileup().size();
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totalCoverage += coverage;
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if (coverage >= targetCoverage)
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return 1;
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return 0;
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}
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public Long reduceInit() { return 0l; }
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public Long reduce(Integer value, Long sum) {
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return value + sum;
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}
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/**
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* Reduces two subtrees together. In this case, the implementation of the tree reduce
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* is exactly the same as the implementation of the single reduce.
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*/
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public Long treeReduce(Long lhs, Long rhs) {
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return lhs + rhs;
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}
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public void onTraversalDone( Long c ) {
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out.println(Math.floor(totalCoverage/totalLoci) + "\t" + (double) c/totalLoci);
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}
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}
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