Walker that calculates the percentage of bases that are covered to at least 20x. Very useful! In oneoffs until someone else thinks it's as useful as I think it is ;)

git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@5714 348d0f76-0448-11de-a6fe-93d51630548a
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carneiro 2011-04-29 19:19:39 +00:00
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package org.broadinstitute.sting.oneoffprojects.walkers;
import org.broadinstitute.sting.commandline.Argument;
import org.broadinstitute.sting.commandline.Output;
import org.broadinstitute.sting.gatk.contexts.AlignmentContext;
import org.broadinstitute.sting.gatk.contexts.ReferenceContext;
import org.broadinstitute.sting.gatk.refdata.RefMetaDataTracker;
import org.broadinstitute.sting.gatk.walkers.LocusWalker;
import org.broadinstitute.sting.gatk.walkers.TreeReducible;
import java.io.PrintStream;
/**
* Walks over the input data set, calculating the total number of covered loci for diagnostic purposes.
* Simplest example of a locus walker.
*/
public class Percent20xCoverage extends LocusWalker<Integer, Long> implements TreeReducible<Long> {
@Output(doc="Write count to this file instead of STDOUT")
PrintStream out;
@Argument(fullName="target_coverage", shortName="coverage", doc="Set the target coverage", required=false)
private int targetCoverage = 20;
private long totalLoci;
private long totalCoverage;
public void initialize () {
totalLoci = 0;
totalCoverage = 0;
}
public Integer map(RefMetaDataTracker tracker, ReferenceContext ref, AlignmentContext context) {
totalLoci++;
int coverage = context.getBasePileup().size();
totalCoverage += coverage;
if (coverage >= targetCoverage)
return 1;
return 0;
}
public Long reduceInit() { return 0l; }
public Long reduce(Integer value, Long sum) {
return value + sum;
}
/**
* Reduces two subtrees together. In this case, the implementation of the tree reduce
* is exactly the same as the implementation of the single reduce.
*/
public Long treeReduce(Long lhs, Long rhs) {
return lhs + rhs;
}
public void onTraversalDone( Long c ) {
out.println(Math.floor(totalCoverage/totalLoci) + "\t" + (double) c/totalLoci);
}
}