From c3ea96d85616a3ce0b4b90d40e2d141b1efd16cf Mon Sep 17 00:00:00 2001 From: Mark DePristo Date: Fri, 2 Sep 2011 08:42:01 -0400 Subject: [PATCH 01/41] Removing many unused functions of unquestionable purpose --- .../sting/utils/QualityUtils.java | 101 ++---------------- 1 file changed, 10 insertions(+), 91 deletions(-) diff --git a/public/java/src/org/broadinstitute/sting/utils/QualityUtils.java b/public/java/src/org/broadinstitute/sting/utils/QualityUtils.java index fad2320fc..093da7dd6 100755 --- a/public/java/src/org/broadinstitute/sting/utils/QualityUtils.java +++ b/public/java/src/org/broadinstitute/sting/utils/QualityUtils.java @@ -9,14 +9,17 @@ import net.sf.samtools.SAMUtils; * @author Kiran Garimella */ public class QualityUtils { - public final static byte MAX_QUAL_SCORE = SAMUtils.MAX_PHRED_SCORE; public final static double MIN_REASONABLE_ERROR = 0.0001; public final static byte MAX_REASONABLE_Q_SCORE = 40; public final static byte MIN_USABLE_Q_SCORE = 6; - public final static int MAPPING_QUALITY_UNAVAILABLE = 255; + private static double qualToErrorProbCache[] = new double[256]; + static { + for (byte i = 0; i < 256; i++) qualToErrorProbCache[i] = qualToErrorProbRaw(i); + } + /** * Private constructor. No instantiating this class! */ @@ -33,10 +36,6 @@ public class QualityUtils { return 1.0 - qualToErrorProb(qual); } - static public double qualToProb(int qual) { - return qualToProb( (double)qual ); - } - static public double qualToProb(double qual) { return 1.0 - Math.pow(10.0, qual/(-10.0)); } @@ -48,10 +47,14 @@ public class QualityUtils { * @param qual a quality score (0-40) * @return a probability (0.0-1.0) */ - static public double qualToErrorProb(byte qual) { + static public double qualToErrorProbRaw(byte qual) { return Math.pow(10.0, ((double) qual)/-10.0); } + static public double qualToErrorProb(byte qual) { + return qualToErrorProbCache[qual]; + } + /** * Convert a probability to a quality score. Note, this is capped at Q40. * @@ -110,88 +113,4 @@ public class QualityUtils { //return (byte) Math.min(qual, maxQual); return (byte) Math.max(Math.min(qual, maxQual), 1); } - - /** - * Compress a base and a probability into a single byte so that it can be output in a SAMRecord's SQ field. - * Note: the highest probability this function can encode is 64%, so this function should only never be used on the best base hypothesis. - * Another note: the probability encoded here gets rounded to the nearest 1%. - * - * @param baseIndex the base index - * @param prob the base probability - * @return a byte containing the index and the probability - */ - static public byte baseAndProbToCompressedQuality(int baseIndex, double prob) { - byte compressedQual = 0; - - compressedQual = (byte) baseIndex; - - byte cprob = (byte) (100.0*prob); - byte qualmask = (byte) 252; - compressedQual += ((cprob << 2) & qualmask); - - return compressedQual; - } - - /** - * From a compressed base, extract the base index (0:A, 1:C, 2:G, 3:T) - * - * @param compressedQual the compressed quality score, as returned by baseAndProbToCompressedQuality - * @return base index - */ - static public int compressedQualityToBaseIndex(byte compressedQual) { - return (int) (compressedQual & 0x3); - } - - /** - * From a compressed base, extract the base probability - * - * @param compressedQual the compressed quality score, as returned by baseAndProbToCompressedQuality - * @return the probability - */ - static public double compressedQualityToProb(byte compressedQual) { - // Because java natives are signed, extra care must be taken to avoid - // shifting a 1 into the sign bit in the implicit promotion of 2 to an int. - int x2 = ((int) compressedQual) & 0xff; - x2 = (x2 >>> 2); - - return ((double) x2)/100.0; - } - - /** - * Return the complement of a compressed quality - * - * @param compressedQual the compressed quality score (as returned by baseAndProbToCompressedQuality) - * @return the complementary compressed quality - */ - static public byte complementCompressedQuality(byte compressedQual) { - int baseIndex = compressedQualityToBaseIndex(compressedQual); - double prob = compressedQualityToProb(compressedQual); - - return baseAndProbToCompressedQuality(BaseUtils.complementIndex(baseIndex), prob); - } - - /** - * Return the reverse complement of a byte array of compressed qualities - * - * @param compressedQuals a byte array of compressed quality scores - * @return the reverse complement of the byte array - */ - static public byte[] reverseComplementCompressedQualityArray(byte[] compressedQuals) { - byte[] rcCompressedQuals = new byte[compressedQuals.length]; - - for (int pos = 0; pos < compressedQuals.length; pos++) { - rcCompressedQuals[compressedQuals.length - pos - 1] = complementCompressedQuality(compressedQuals[pos]); - } - - return rcCompressedQuals; - } - - /** - * Return the reverse of a byte array of qualities (compressed or otherwise) - * @param quals the array of bytes to be reversed - * @return the reverse of the quality array - */ - static public byte[] reverseQualityArray( byte[] quals ) { - return Utils.reverse(quals); // no sense in duplicating functionality - } } From c57198a1b998ba25b7facac526cafa04f9b8f77a Mon Sep 17 00:00:00 2001 From: Mark DePristo Date: Fri, 2 Sep 2011 08:46:17 -0400 Subject: [PATCH 02/41] Optimizations in VCFCodec -- Don't create an empty LinkedHashSet() for PASS fields. Just return Collections.emptySet() instead. -- For filter fields with actual values, returns an unmodifiableSet instead of one that can be changed --- .../broadinstitute/sting/utils/codecs/vcf/VCFCodec.java | 9 ++++----- 1 file changed, 4 insertions(+), 5 deletions(-) diff --git a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/VCFCodec.java b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/VCFCodec.java index fa030ef5f..cd320b332 100755 --- a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/VCFCodec.java +++ b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/VCFCodec.java @@ -110,11 +110,8 @@ public class VCFCodec extends AbstractVCFCodec { if ( filterString.equals(VCFConstants.UNFILTERED) ) return null; - // empty set for passes filters - LinkedHashSet fFields = new LinkedHashSet(); - if ( filterString.equals(VCFConstants.PASSES_FILTERS_v4) ) - return fFields; + return Collections.emptySet(); if ( filterString.equals(VCFConstants.PASSES_FILTERS_v3) ) generateException(VCFConstants.PASSES_FILTERS_v3 + " is an invalid filter name in vcf4"); if ( filterString.length() == 0 ) @@ -124,6 +121,8 @@ public class VCFCodec extends AbstractVCFCodec { if ( filterHash.containsKey(filterString) ) return filterHash.get(filterString); + // empty set for passes filters + LinkedHashSet fFields = new LinkedHashSet(); // otherwise we have to parse and cache the value if ( filterString.indexOf(VCFConstants.FILTER_CODE_SEPARATOR) == -1 ) fFields.add(filterString); @@ -132,7 +131,7 @@ public class VCFCodec extends AbstractVCFCodec { filterHash.put(filterString, fFields); - return fFields; + return Collections.unmodifiableSet(fFields); } From 82f213177730123def312b6a5b64878f7f665769 Mon Sep 17 00:00:00 2001 From: Mark DePristo Date: Fri, 2 Sep 2011 12:27:11 -0400 Subject: [PATCH 03/41] Simplied getAttributeAsX interfaces -- Removed versions getAttribriteAsX(key) that except on not having the value. -- Removed version that getAttributeAsXNoException(key) -- The only available assessors are now getAttributeAsX(key, default). -- This single accessors properly handle their argument types, so if the value is a double it is returned directly for getAttributeAsDouble(), or if it's a string it's converted to a double. If the key isn't found, default is returned. --- .../gatk/walkers/annotator/SBByDepth.java | 2 +- .../indels/HaplotypeIndelErrorModel.java | 2 +- .../gatk/walkers/phasing/PhasingRead.java | 2 +- .../walkers/phasing/RefSeqDataParser.java | 10 ++-- .../varianteval/evaluators/CountVariants.java | 6 +-- .../evaluators/GenotypePhasingEvaluator.java | 3 +- .../evaluators/SimpleMetricsByAC.java | 2 +- .../evaluators/TiTvVariantEvaluator.java | 2 +- .../evaluators/ValidationReport.java | 2 +- .../stratifications/AlleleCount.java | 2 +- .../stratifications/AlleleFrequency.java | 2 +- .../stratifications/Degeneracy.java | 10 ++-- .../stratifications/FunctionalClass.java | 4 +- .../VQSRCalibrationCurve.java | 4 +- .../walkers/variantutils/SelectVariants.java | 2 +- .../walkers/variantutils/VariantsToTable.java | 2 +- .../sting/utils/codecs/vcf/VCFCodec.java | 14 +++-- .../sting/utils/variantcontext/Genotype.java | 9 ---- .../InferredGeneticContext.java | 53 ++++++++++++------- .../utils/variantcontext/VariantContext.java | 10 ---- .../variantcontext/VariantContextUtils.java | 16 +++--- 21 files changed, 79 insertions(+), 80 deletions(-) diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/annotator/SBByDepth.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/annotator/SBByDepth.java index 180bed24d..d2c4d24ab 100755 --- a/public/java/src/org/broadinstitute/sting/gatk/walkers/annotator/SBByDepth.java +++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/annotator/SBByDepth.java @@ -26,7 +26,7 @@ public class SBByDepth extends AnnotationByDepth { if (!vc.hasAttribute(VCFConstants.STRAND_BIAS_KEY)) return null; - double sBias = Double.valueOf(vc.getAttributeAsString(VCFConstants.STRAND_BIAS_KEY)); + double sBias = vc.getAttributeAsDouble(VCFConstants.STRAND_BIAS_KEY, -1); final Map genotypes = vc.getGenotypes(); if ( genotypes == null || genotypes.size() == 0 ) diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/indels/HaplotypeIndelErrorModel.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/indels/HaplotypeIndelErrorModel.java index e68aa31e0..232e468f9 100755 --- a/public/java/src/org/broadinstitute/sting/gatk/walkers/indels/HaplotypeIndelErrorModel.java +++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/indels/HaplotypeIndelErrorModel.java @@ -73,7 +73,7 @@ public class HaplotypeIndelErrorModel { baseMatchArray = new double[MAX_CACHED_QUAL+1]; baseMismatchArray = new double[MAX_CACHED_QUAL+1]; for (int k=1; k <= MAX_CACHED_QUAL; k++) { - double baseProb = QualityUtils.qualToProb(k); + double baseProb = QualityUtils.qualToProb((byte)k); baseMatchArray[k] = probToQual(baseProb); diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/phasing/PhasingRead.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/phasing/PhasingRead.java index a56c9e21e..63fb33295 100644 --- a/public/java/src/org/broadinstitute/sting/gatk/walkers/phasing/PhasingRead.java +++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/phasing/PhasingRead.java @@ -37,7 +37,7 @@ public class PhasingRead extends BaseArray { public PhasingRead(int length, int mappingQual) { super(length); - this.mappingProb = new PreciseNonNegativeDouble(QualityUtils.qualToProb(mappingQual)); + this.mappingProb = new PreciseNonNegativeDouble(QualityUtils.qualToProb((byte)mappingQual)); this.baseProbs = new PreciseNonNegativeDouble[length]; Arrays.fill(this.baseProbs, null); diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/phasing/RefSeqDataParser.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/phasing/RefSeqDataParser.java index 55da1c152..f94140814 100644 --- a/public/java/src/org/broadinstitute/sting/gatk/walkers/phasing/RefSeqDataParser.java +++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/phasing/RefSeqDataParser.java @@ -44,12 +44,12 @@ public class RefSeqDataParser { String nameKeyToUseMultiplePrefix = nameKeyToUse + "_"; Map entriesToNames = new HashMap(); - Integer numRecords = vc.getAttributeAsIntegerNoException(NUM_RECORDS_KEY); - if (numRecords != null) { + int numRecords = vc.getAttributeAsInt(NUM_RECORDS_KEY, -1); + if (numRecords != -1) { boolean done = false; if (numRecords == 1) { // Check if perhaps the single record doesn't end with "_1": - String name = vc.getAttributeAsStringNoException(nameKeyToUse); + String name = vc.getAttributeAsString(nameKeyToUse, null); if (name != null) { entriesToNames.put(nameKeyToUse, name); done = true; @@ -59,14 +59,14 @@ public class RefSeqDataParser { if (!done) { for (int i = 1; i <= numRecords; i++) { String key = nameKeyToUseMultiplePrefix + i; - String name = vc.getAttributeAsStringNoException(key); + String name = vc.getAttributeAsString(key, null); if (name != null) entriesToNames.put(key, name); } } } else { // no entry with the # of records: - String name = vc.getAttributeAsStringNoException(nameKeyToUse); + String name = vc.getAttributeAsString(nameKeyToUse, null); if (name != null) { entriesToNames.put(nameKeyToUse, name); } diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/evaluators/CountVariants.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/evaluators/CountVariants.java index 59ef3d992..fd379dfda 100755 --- a/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/evaluators/CountVariants.java +++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/evaluators/CountVariants.java @@ -109,12 +109,12 @@ public class CountVariants extends VariantEvaluator implements StandardEval { case SNP: nVariantLoci++; nSNPs++; - if (vc1.getAttributeAsBoolean("ISSINGLETON")) nSingletons++; + if (vc1.getAttributeAsBoolean("ISSINGLETON", false)) nSingletons++; break; case MNP: nVariantLoci++; nMNPs++; - if (vc1.getAttributeAsBoolean("ISSINGLETON")) nSingletons++; + if (vc1.getAttributeAsBoolean("ISSINGLETON", false)) nSingletons++; break; case INDEL: nVariantLoci++; @@ -136,7 +136,7 @@ public class CountVariants extends VariantEvaluator implements StandardEval { String refStr = vc1.getReference().getBaseString().toUpperCase(); - String aaStr = vc1.hasAttribute("ANCESTRALALLELE") ? vc1.getAttributeAsString("ANCESTRALALLELE").toUpperCase() : null; + String aaStr = vc1.hasAttribute("ANCESTRALALLELE") ? vc1.getAttributeAsString("ANCESTRALALLELE", null).toUpperCase() : null; // if (aaStr.equals(".")) { // aaStr = refStr; // } diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/evaluators/GenotypePhasingEvaluator.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/evaluators/GenotypePhasingEvaluator.java index a476a2680..e69dbfb28 100755 --- a/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/evaluators/GenotypePhasingEvaluator.java +++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/evaluators/GenotypePhasingEvaluator.java @@ -219,7 +219,8 @@ public class GenotypePhasingEvaluator extends VariantEvaluator { } public static Double getPQ(Genotype gt) { - return gt.getAttributeAsDoubleNoException(ReadBackedPhasingWalker.PQ_KEY); + Double d = gt.getAttributeAsDouble(ReadBackedPhasingWalker.PQ_KEY, -1); + return d == -1 ? null : d; } public static boolean topMatchesTop(AllelePair b1, AllelePair b2) { diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/evaluators/SimpleMetricsByAC.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/evaluators/SimpleMetricsByAC.java index d466645ea..38cbf1c45 100755 --- a/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/evaluators/SimpleMetricsByAC.java +++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/evaluators/SimpleMetricsByAC.java @@ -120,7 +120,7 @@ public class SimpleMetricsByAC extends VariantEvaluator implements StandardEval if ( eval.hasGenotypes() ) ac = eval.getChromosomeCount(eval.getAlternateAllele(0)); else if ( eval.hasAttribute("AC") ) { - ac = Integer.valueOf(eval.getAttributeAsString("AC")); + ac = eval.getAttributeAsInt("AC", -1); } if ( ac != -1 ) { diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/evaluators/TiTvVariantEvaluator.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/evaluators/TiTvVariantEvaluator.java index be957abd7..ee58012a0 100755 --- a/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/evaluators/TiTvVariantEvaluator.java +++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/evaluators/TiTvVariantEvaluator.java @@ -50,7 +50,7 @@ public class TiTvVariantEvaluator extends VariantEvaluator implements StandardEv } String refStr = vc.getReference().getBaseString().toUpperCase(); - String aaStr = vc.getAttributeAsString("ANCESTRALALLELE").toUpperCase(); + String aaStr = vc.getAttributeAsString("ANCESTRALALLELE", null).toUpperCase(); if (aaStr != null && !aaStr.equalsIgnoreCase("null") && !aaStr.equals(".")) { BaseUtils.BaseSubstitutionType aaSubType = BaseUtils.SNPSubstitutionType(aaStr.getBytes()[0], vc.getAlternateAllele(0).getBases()[0]); diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/evaluators/ValidationReport.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/evaluators/ValidationReport.java index 9c331b577..7fa56785b 100755 --- a/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/evaluators/ValidationReport.java +++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/evaluators/ValidationReport.java @@ -130,7 +130,7 @@ public class ValidationReport extends VariantEvaluator implements StandardEval { //// System.out.printf(" ac = %d%n", ac); } else - ac = vc.getAttributeAsInt(VCFConstants.ALLELE_COUNT_KEY); + ac = vc.getAttributeAsInt(VCFConstants.ALLELE_COUNT_KEY, 0); return ac > 0 ? SiteStatus.POLY : SiteStatus.MONO; } else if ( vc.hasGenotypes() ) { return vc.isPolymorphic() ? SiteStatus.POLY : SiteStatus.MONO; diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/stratifications/AlleleCount.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/stratifications/AlleleCount.java index 5cdea4e00..56b06d032 100755 --- a/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/stratifications/AlleleCount.java +++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/stratifications/AlleleCount.java @@ -45,7 +45,7 @@ public class AlleleCount extends VariantStratifier { if (eval != null) { int AC = -1; if ( eval.hasAttribute("AC") && eval.getAttribute("AC") instanceof Integer ) { - AC = eval.getAttributeAsInt("AC"); + AC = eval.getAttributeAsInt("AC", 0); } else if ( eval.isVariant() ) { for (Allele allele : eval.getAlternateAlleles()) AC = Math.max(AC, eval.getChromosomeCount(allele)); diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/stratifications/AlleleFrequency.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/stratifications/AlleleFrequency.java index 96d9f30ec..ac1ee9e0e 100755 --- a/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/stratifications/AlleleFrequency.java +++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/stratifications/AlleleFrequency.java @@ -28,7 +28,7 @@ public class AlleleFrequency extends VariantStratifier { if (eval != null) { try { - relevantStates.add(String.format("%.3f", (5.0 * MathUtils.round(eval.getAttributeAsDouble("AF") / 5.0, 3)))); + relevantStates.add(String.format("%.3f", (5.0 * MathUtils.round(eval.getAttributeAsDouble("AF", 0.0) / 5.0, 3)))); } catch (Exception e) { return relevantStates; } diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/stratifications/Degeneracy.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/stratifications/Degeneracy.java index cc878e975..06ac05ec8 100755 --- a/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/stratifications/Degeneracy.java +++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/stratifications/Degeneracy.java @@ -92,8 +92,8 @@ public class Degeneracy extends VariantStratifier { Integer frame = null; if (eval.hasAttribute("refseq.functionalClass")) { - aa = eval.getAttributeAsString("refseq.variantAA"); - frame = eval.getAttributeAsInt("refseq.frame"); + aa = eval.getAttributeAsString("refseq.variantAA", null); + frame = eval.getAttributeAsInt("refseq.frame", 0); } else if (eval.hasAttribute("refseq.functionalClass_1")) { int annotationId = 1; String key; @@ -101,7 +101,7 @@ public class Degeneracy extends VariantStratifier { do { key = String.format("refseq.functionalClass_%d", annotationId); - String newtype = eval.getAttributeAsString(key); + String newtype = eval.getAttributeAsString(key, null); if ( newtype != null && ( type == null || @@ -111,13 +111,13 @@ public class Degeneracy extends VariantStratifier { type = newtype; String aakey = String.format("refseq.variantAA_%d", annotationId); - aa = eval.getAttributeAsString(aakey); + aa = eval.getAttributeAsString(aakey, null); if (aa != null) { String framekey = String.format("refseq.frame_%d", annotationId); if (eval.hasAttribute(framekey)) { - frame = eval.getAttributeAsInt(framekey); + frame = eval.getAttributeAsInt(framekey, 0); } } } diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/stratifications/FunctionalClass.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/stratifications/FunctionalClass.java index 0de871fe6..4af12fbd1 100755 --- a/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/stratifications/FunctionalClass.java +++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/varianteval/stratifications/FunctionalClass.java @@ -32,7 +32,7 @@ public class FunctionalClass extends VariantStratifier { String type = null; if (eval.hasAttribute("refseq.functionalClass")) { - type = eval.getAttributeAsString("refseq.functionalClass"); + type = eval.getAttributeAsString("refseq.functionalClass", null); } else if (eval.hasAttribute("refseq.functionalClass_1")) { int annotationId = 1; String key; @@ -40,7 +40,7 @@ public class FunctionalClass extends VariantStratifier { do { key = String.format("refseq.functionalClass_%d", annotationId); - String newtype = eval.getAttributeAsString(key); + String newtype = eval.getAttributeAsString(key, null); if ( newtype != null && !newtype.equalsIgnoreCase("null") && ( type == null || diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/variantrecalibration/VQSRCalibrationCurve.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/variantrecalibration/VQSRCalibrationCurve.java index bc7252ec2..04ba3ff14 100644 --- a/public/java/src/org/broadinstitute/sting/gatk/walkers/variantrecalibration/VQSRCalibrationCurve.java +++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/variantrecalibration/VQSRCalibrationCurve.java @@ -115,7 +115,7 @@ public class VQSRCalibrationCurve { if ( vc.isFiltered() ) return 0.0; else if ( vc.hasAttribute(VQSRQualKey) ) { - double qual = vc.getAttributeAsDouble(VQSRQualKey); + double qual = vc.getAttributeAsDouble(VQSRQualKey, 0.0); return probTrueVariant(qual); } else { throw new UserException.VariantContextMissingRequiredField(VQSRQualKey, vc); @@ -143,7 +143,7 @@ public class VQSRCalibrationCurve { for ( int i = 0; i < log10Likelihoods.length; i++) { double p = Math.pow(10, log10Likelihoods[i]); double q = alpha * p + (1-alpha) * noInfoPr; - if ( DEBUG ) System.out.printf(" vqslod = %.2f, p = %.2e, alpha = %.2e, q = %.2e%n", vc.getAttributeAsDouble(VQSRQualKey), p, alpha, q); + if ( DEBUG ) System.out.printf(" vqslod = %.2f, p = %.2e, alpha = %.2e, q = %.2e%n", vc.getAttributeAsDouble(VQSRQualKey, 0.0), p, alpha, q); updated[i] = Math.log10(q); } diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/variantutils/SelectVariants.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/variantutils/SelectVariants.java index bb3cd82a1..ceafb0cf5 100755 --- a/public/java/src/org/broadinstitute/sting/gatk/walkers/variantutils/SelectVariants.java +++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/variantutils/SelectVariants.java @@ -575,7 +575,7 @@ public class SelectVariants extends RodWalker { // ok we have a comp VC and we need to match the AF spectrum of inputAFRodName. // We then pick a variant with probablity AF*desiredFraction if ( sub.hasAttribute(VCFConstants.ALLELE_FREQUENCY_KEY) ) { - String afo = sub.getAttributeAsString(VCFConstants.ALLELE_FREQUENCY_KEY); + String afo = sub.getAttributeAsString(VCFConstants.ALLELE_FREQUENCY_KEY, null); double af; double afBoost = 1.0; diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/variantutils/VariantsToTable.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/variantutils/VariantsToTable.java index 2a877fb09..aafbe4db4 100755 --- a/public/java/src/org/broadinstitute/sting/gatk/walkers/variantutils/VariantsToTable.java +++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/variantutils/VariantsToTable.java @@ -192,7 +192,7 @@ public class VariantsToTable extends RodWalker { if ( getters.containsKey(field) ) { val = getters.get(field).get(vc); } else if ( vc.hasAttribute(field) ) { - val = vc.getAttributeAsString(field); + val = vc.getAttributeAsString(field, null); } else if ( isWildCard(field) ) { Set wildVals = new HashSet(); for ( Map.Entry elt : vc.getAttributes().entrySet()) { diff --git a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/VCFCodec.java b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/VCFCodec.java index cd320b332..94e40fc98 100755 --- a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/VCFCodec.java +++ b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/VCFCodec.java @@ -105,7 +105,10 @@ public class VCFCodec extends AbstractVCFCodec { * @return a set of the filters applied or null if filters were not applied to the record (e.g. as per the missing value in a VCF) */ protected Set parseFilters(String filterString) { + return parseFilters(filterHash, lineNo, filterString); + } + public static Set parseFilters(final Map> cache, final int lineNo, final String filterString) { // null for unfiltered if ( filterString.equals(VCFConstants.UNFILTERED) ) return null; @@ -113,13 +116,13 @@ public class VCFCodec extends AbstractVCFCodec { if ( filterString.equals(VCFConstants.PASSES_FILTERS_v4) ) return Collections.emptySet(); if ( filterString.equals(VCFConstants.PASSES_FILTERS_v3) ) - generateException(VCFConstants.PASSES_FILTERS_v3 + " is an invalid filter name in vcf4"); + generateException(VCFConstants.PASSES_FILTERS_v3 + " is an invalid filter name in vcf4", lineNo); if ( filterString.length() == 0 ) - generateException("The VCF specification requires a valid filter status"); + generateException("The VCF specification requires a valid filter status", lineNo); // do we have the filter string cached? - if ( filterHash.containsKey(filterString) ) - return filterHash.get(filterString); + if ( cache != null && cache.containsKey(filterString) ) + return Collections.unmodifiableSet(cache.get(filterString)); // empty set for passes filters LinkedHashSet fFields = new LinkedHashSet(); @@ -129,7 +132,8 @@ public class VCFCodec extends AbstractVCFCodec { else fFields.addAll(Arrays.asList(filterString.split(VCFConstants.FILTER_CODE_SEPARATOR))); - filterHash.put(filterString, fFields); + fFields = fFields; + if ( cache != null ) cache.put(filterString, fFields); return Collections.unmodifiableSet(fFields); } diff --git a/public/java/src/org/broadinstitute/sting/utils/variantcontext/Genotype.java b/public/java/src/org/broadinstitute/sting/utils/variantcontext/Genotype.java index fdf3d97db..85d752003 100755 --- a/public/java/src/org/broadinstitute/sting/utils/variantcontext/Genotype.java +++ b/public/java/src/org/broadinstitute/sting/utils/variantcontext/Genotype.java @@ -293,17 +293,8 @@ public class Genotype { return commonInfo.getAttribute(key, defaultValue); } - public String getAttributeAsString(String key) { return commonInfo.getAttributeAsString(key); } public String getAttributeAsString(String key, String defaultValue) { return commonInfo.getAttributeAsString(key, defaultValue); } - public int getAttributeAsInt(String key) { return commonInfo.getAttributeAsInt(key); } public int getAttributeAsInt(String key, int defaultValue) { return commonInfo.getAttributeAsInt(key, defaultValue); } - public double getAttributeAsDouble(String key) { return commonInfo.getAttributeAsDouble(key); } public double getAttributeAsDouble(String key, double defaultValue) { return commonInfo.getAttributeAsDouble(key, defaultValue); } - public boolean getAttributeAsBoolean(String key) { return commonInfo.getAttributeAsBoolean(key); } public boolean getAttributeAsBoolean(String key, boolean defaultValue) { return commonInfo.getAttributeAsBoolean(key, defaultValue); } - - public Integer getAttributeAsIntegerNoException(String key) { return commonInfo.getAttributeAsIntegerNoException(key); } - public Double getAttributeAsDoubleNoException(String key) { return commonInfo.getAttributeAsDoubleNoException(key); } - public String getAttributeAsStringNoException(String key) { return commonInfo.getAttributeAsStringNoException(key); } - public Boolean getAttributeAsBooleanNoException(String key) { return commonInfo.getAttributeAsBooleanNoException(key); } } \ No newline at end of file diff --git a/public/java/src/org/broadinstitute/sting/utils/variantcontext/InferredGeneticContext.java b/public/java/src/org/broadinstitute/sting/utils/variantcontext/InferredGeneticContext.java index 3d162adb0..4266fb4b5 100755 --- a/public/java/src/org/broadinstitute/sting/utils/variantcontext/InferredGeneticContext.java +++ b/public/java/src/org/broadinstitute/sting/utils/variantcontext/InferredGeneticContext.java @@ -204,27 +204,40 @@ public final class InferredGeneticContext { return defaultValue; } -// public AttributedObject getAttributes(Collection keys) { -// AttributedObject selected = new AttributedObject(); -// -// for ( Object key : keys ) -// selected.putAttribute(key, this.getAttribute(key)); -// -// return selected; -// } + public String getAttributeAsString(String key, String defaultValue) { + Object x = getAttribute(key); + if ( x == null ) return defaultValue; + if ( x instanceof String ) return (String)x; + return String.valueOf(x); // throws an exception if this isn't a string + } - public String getAttributeAsString(String key) { return (String.valueOf(getAttribute(key))); } // **NOTE**: will turn a null Object into the String "null" - public int getAttributeAsInt(String key) { Object x = getAttribute(key); return x instanceof Integer ? (Integer)x : Integer.valueOf((String)x); } - public double getAttributeAsDouble(String key) { Object x = getAttribute(key); return x instanceof Double ? (Double)x : Double.valueOf((String)x); } - public boolean getAttributeAsBoolean(String key) { Object x = getAttribute(key); return x instanceof Boolean ? (Boolean)x : Boolean.valueOf((String)x); } + public int getAttributeAsInt(String key, int defaultValue) { + Object x = getAttribute(key); + if ( x == null ) return defaultValue; + if ( x instanceof Integer ) return (Integer)x; + return Integer.valueOf((String)x); // throws an exception if this isn't a string + } - public String getAttributeAsString(String key, String defaultValue) { return (String)getAttribute(key, defaultValue); } - public int getAttributeAsInt(String key, int defaultValue) { return (Integer)getAttribute(key, defaultValue); } - public double getAttributeAsDouble(String key, double defaultValue) { return (Double)getAttribute(key, defaultValue); } - public boolean getAttributeAsBoolean(String key, boolean defaultValue){ return (Boolean)getAttribute(key, defaultValue); } + public double getAttributeAsDouble(String key, double defaultValue) { + Object x = getAttribute(key); + if ( x == null ) return defaultValue; + if ( x instanceof Double ) return (Double)x; + return Double.valueOf((String)x); // throws an exception if this isn't a string + } - public Integer getAttributeAsIntegerNoException(String key) { try {return getAttributeAsInt(key);} catch (Exception e) {return null;} } - public Double getAttributeAsDoubleNoException(String key) { try {return getAttributeAsDouble(key);} catch (Exception e) {return null;} } - public String getAttributeAsStringNoException(String key) { if (getAttribute(key) == null) return null; return getAttributeAsString(key); } - public Boolean getAttributeAsBooleanNoException(String key) { try {return getAttributeAsBoolean(key);} catch (Exception e) {return null;} } + public boolean getAttributeAsBoolean(String key, boolean defaultValue) { + Object x = getAttribute(key); + if ( x == null ) return defaultValue; + if ( x instanceof Boolean ) return (Boolean)x; + return Boolean.valueOf((String)x); // throws an exception if this isn't a string + } + +// public String getAttributeAsString(String key) { return (String.valueOf(getAttribute(key))); } // **NOTE**: will turn a null Object into the String "null" +// public int getAttributeAsInt(String key) { Object x = getAttribute(key); return x instanceof Integer ? (Integer)x : Integer.valueOf((String)x); } +// public double getAttributeAsDouble(String key) { Object x = getAttribute(key); return x instanceof Double ? (Double)x : Double.valueOf((String)x); } +// public boolean getAttributeAsBoolean(String key) { Object x = getAttribute(key); return x instanceof Boolean ? (Boolean)x : Boolean.valueOf((String)x); } +// public Integer getAttributeAsIntegerNoException(String key) { try {return getAttributeAsInt(key);} catch (Exception e) {return null;} } +// public Double getAttributeAsDoubleNoException(String key) { try {return getAttributeAsDouble(key);} catch (Exception e) {return null;} } +// public String getAttributeAsStringNoException(String key) { if (getAttribute(key) == null) return null; return getAttributeAsString(key); } +// public Boolean getAttributeAsBooleanNoException(String key) { try {return getAttributeAsBoolean(key);} catch (Exception e) {return null;} } } \ No newline at end of file diff --git a/public/java/src/org/broadinstitute/sting/utils/variantcontext/VariantContext.java b/public/java/src/org/broadinstitute/sting/utils/variantcontext/VariantContext.java index 673fe4529..e6637a5d9 100755 --- a/public/java/src/org/broadinstitute/sting/utils/variantcontext/VariantContext.java +++ b/public/java/src/org/broadinstitute/sting/utils/variantcontext/VariantContext.java @@ -666,21 +666,11 @@ public class VariantContext implements Feature { // to enable tribble intergrati return commonInfo.getAttribute(key, defaultValue); } - public String getAttributeAsString(String key) { return commonInfo.getAttributeAsString(key); } public String getAttributeAsString(String key, String defaultValue) { return commonInfo.getAttributeAsString(key, defaultValue); } - public int getAttributeAsInt(String key) { return commonInfo.getAttributeAsInt(key); } public int getAttributeAsInt(String key, int defaultValue) { return commonInfo.getAttributeAsInt(key, defaultValue); } - public double getAttributeAsDouble(String key) { return commonInfo.getAttributeAsDouble(key); } public double getAttributeAsDouble(String key, double defaultValue) { return commonInfo.getAttributeAsDouble(key, defaultValue); } - public boolean getAttributeAsBoolean(String key) { return commonInfo.getAttributeAsBoolean(key); } public boolean getAttributeAsBoolean(String key, boolean defaultValue) { return commonInfo.getAttributeAsBoolean(key, defaultValue); } - public Integer getAttributeAsIntegerNoException(String key) { return commonInfo.getAttributeAsIntegerNoException(key); } - public Double getAttributeAsDoubleNoException(String key) { return commonInfo.getAttributeAsDoubleNoException(key); } - public String getAttributeAsStringNoException(String key) { return commonInfo.getAttributeAsStringNoException(key); } - public Boolean getAttributeAsBooleanNoException(String key) { return commonInfo.getAttributeAsBooleanNoException(key); } - - // --------------------------------------------------------------------------------------------------------- // // Working with alleles diff --git a/public/java/src/org/broadinstitute/sting/utils/variantcontext/VariantContextUtils.java b/public/java/src/org/broadinstitute/sting/utils/variantcontext/VariantContextUtils.java index 986d6305c..d5c541b19 100755 --- a/public/java/src/org/broadinstitute/sting/utils/variantcontext/VariantContextUtils.java +++ b/public/java/src/org/broadinstitute/sting/utils/variantcontext/VariantContextUtils.java @@ -565,11 +565,11 @@ public class VariantContextUtils { // special case DP (add it up) and ID (just preserve it) // if (vc.hasAttribute(VCFConstants.DEPTH_KEY)) - depth += Integer.valueOf(vc.getAttributeAsString(VCFConstants.DEPTH_KEY)); + depth += vc.getAttributeAsInt(VCFConstants.DEPTH_KEY, 0); if (rsID == null && vc.hasID()) rsID = vc.getID(); if (mergeInfoWithMaxAC && vc.hasAttribute(VCFConstants.ALLELE_COUNT_KEY)) { - String rawAlleleCounts = vc.getAttributeAsString(VCFConstants.ALLELE_COUNT_KEY); + String rawAlleleCounts = vc.getAttributeAsString(VCFConstants.ALLELE_COUNT_KEY, null); // lets see if the string contains a , separator if (rawAlleleCounts.contains(VCFConstants.INFO_FIELD_ARRAY_SEPARATOR)) { List alleleCountArray = Arrays.asList(rawAlleleCounts.substring(1, rawAlleleCounts.length() - 1).split(VCFConstants.INFO_FIELD_ARRAY_SEPARATOR)); @@ -1147,9 +1147,7 @@ public class VariantContextUtils { for (String orAttrib : MERGE_OR_ATTRIBS) { boolean attribVal = false; for (VariantContext vc : vcList) { - Boolean val = vc.getAttributeAsBooleanNoException(orAttrib); - if (val != null) - attribVal = (attribVal || val); + attribVal = vc.getAttributeAsBoolean(orAttrib, false); if (attribVal) // already true, so no reason to continue: break; } @@ -1159,7 +1157,7 @@ public class VariantContextUtils { // Merge ID fields: String iDVal = null; for (VariantContext vc : vcList) { - String val = vc.getAttributeAsStringNoException(VariantContext.ID_KEY); + String val = vc.getAttributeAsString(VariantContext.ID_KEY, null); if (val != null && !val.equals(VCFConstants.EMPTY_ID_FIELD)) { if (iDVal == null) iDVal = val; @@ -1239,8 +1237,10 @@ public class VariantContextUtils { public PhaseAndQuality(Genotype gt) { this.isPhased = gt.isPhased(); - if (this.isPhased) - this.PQ = gt.getAttributeAsDoubleNoException(ReadBackedPhasingWalker.PQ_KEY); + if (this.isPhased) { + this.PQ = gt.getAttributeAsDouble(ReadBackedPhasingWalker.PQ_KEY, -1); + if ( this.PQ == -1 ) this.PQ = null; + } } } From 124ef6c4834d8a948629291762b7f9d7d9696d50 Mon Sep 17 00:00:00 2001 From: Mark DePristo Date: Fri, 2 Sep 2011 21:12:28 -0400 Subject: [PATCH 04/41] MISSING_VALUE now gets defaultValue in getAttribute functions --- .../sting/utils/variantcontext/InferredGeneticContext.java | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/public/java/src/org/broadinstitute/sting/utils/variantcontext/InferredGeneticContext.java b/public/java/src/org/broadinstitute/sting/utils/variantcontext/InferredGeneticContext.java index 4266fb4b5..bf16cd1cf 100755 --- a/public/java/src/org/broadinstitute/sting/utils/variantcontext/InferredGeneticContext.java +++ b/public/java/src/org/broadinstitute/sting/utils/variantcontext/InferredGeneticContext.java @@ -1,6 +1,8 @@ package org.broadinstitute.sting.utils.variantcontext; +import org.broadinstitute.sting.utils.codecs.vcf.VCFConstants; + import java.util.*; @@ -213,7 +215,7 @@ public final class InferredGeneticContext { public int getAttributeAsInt(String key, int defaultValue) { Object x = getAttribute(key); - if ( x == null ) return defaultValue; + if ( x == null || x == VCFConstants.MISSING_VALUE_v4 ) return defaultValue; if ( x instanceof Integer ) return (Integer)x; return Integer.valueOf((String)x); // throws an exception if this isn't a string } From 03aa04e37c8a2cf31d650cba6a8c703fbb033978 Mon Sep 17 00:00:00 2001 From: Mark DePristo Date: Fri, 2 Sep 2011 21:13:08 -0400 Subject: [PATCH 05/41] Simple refactoring to make formating functions public --- .../sting/utils/codecs/vcf/AbstractVCFCodec.java | 2 +- .../sting/utils/codecs/vcf/StandardVCFWriter.java | 11 +++++++++-- .../sting/utils/codecs/vcf/VCFCodec.java | 2 +- 3 files changed, 11 insertions(+), 4 deletions(-) diff --git a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/AbstractVCFCodec.java b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/AbstractVCFCodec.java index bb212e128..624d06a71 100755 --- a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/AbstractVCFCodec.java +++ b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/AbstractVCFCodec.java @@ -227,7 +227,7 @@ public abstract class AbstractVCFCodec implements FeatureCodec, NameAwareCodec, throw new UserException.MalformedVCF(message, lineNo); } - private static void generateException(String message, int lineNo) { + protected static void generateException(String message, int lineNo) { throw new UserException.MalformedVCF(message, lineNo); } diff --git a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/StandardVCFWriter.java b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/StandardVCFWriter.java index d3705813c..e28cd7598 100755 --- a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/StandardVCFWriter.java +++ b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/StandardVCFWriter.java @@ -275,7 +275,7 @@ public class StandardVCFWriter implements VCFWriter { mWriter.write(VCFConstants.FIELD_SEPARATOR); // FILTER - String filters = vc.isFiltered() ? ParsingUtils.join(";", ParsingUtils.sortList(vc.getFilters())) : (filtersWereAppliedToContext || vc.filtersWereApplied() ? VCFConstants.PASSES_FILTERS_v4 : VCFConstants.UNFILTERED); + String filters = getFilterString(vc, filtersWereAppliedToContext); mWriter.write(filters); mWriter.write(VCFConstants.FIELD_SEPARATOR); @@ -319,7 +319,14 @@ public class StandardVCFWriter implements VCFWriter { } catch (IOException e) { throw new RuntimeException("Unable to write the VCF object to " + locationString()); } + } + public static final String getFilterString(final VariantContext vc) { + return getFilterString(vc, false); + } + + public static final String getFilterString(final VariantContext vc, boolean forcePASS) { + return vc.isFiltered() ? ParsingUtils.join(";", ParsingUtils.sortList(vc.getFilters())) : (forcePASS || vc.filtersWereApplied() ? VCFConstants.PASSES_FILTERS_v4 : VCFConstants.UNFILTERED); } private String getQualValue(double qual) { @@ -462,7 +469,7 @@ public class StandardVCFWriter implements VCFWriter { mWriter.write(encoding); } - private static String formatVCFField(Object val) { + public static String formatVCFField(Object val) { String result; if ( val == null ) result = VCFConstants.MISSING_VALUE_v4; diff --git a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/VCFCodec.java b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/VCFCodec.java index 94e40fc98..42ea05355 100755 --- a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/VCFCodec.java +++ b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/VCFCodec.java @@ -118,7 +118,7 @@ public class VCFCodec extends AbstractVCFCodec { if ( filterString.equals(VCFConstants.PASSES_FILTERS_v3) ) generateException(VCFConstants.PASSES_FILTERS_v3 + " is an invalid filter name in vcf4", lineNo); if ( filterString.length() == 0 ) - generateException("The VCF specification requires a valid filter status", lineNo); + generateException("The VCF specification requires a valid filter status: filter was " + filterString, lineNo); // do we have the filter string cached? if ( cache != null && cache.containsKey(filterString) ) From 048202d18e42444e47e6237246b31d24c57dec9a Mon Sep 17 00:00:00 2001 From: Mark DePristo Date: Fri, 2 Sep 2011 21:13:28 -0400 Subject: [PATCH 06/41] Bugfix for cached quals --- .../java/src/org/broadinstitute/sting/utils/QualityUtils.java | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/public/java/src/org/broadinstitute/sting/utils/QualityUtils.java b/public/java/src/org/broadinstitute/sting/utils/QualityUtils.java index 093da7dd6..19e03a19d 100755 --- a/public/java/src/org/broadinstitute/sting/utils/QualityUtils.java +++ b/public/java/src/org/broadinstitute/sting/utils/QualityUtils.java @@ -17,7 +17,7 @@ public class QualityUtils { private static double qualToErrorProbCache[] = new double[256]; static { - for (byte i = 0; i < 256; i++) qualToErrorProbCache[i] = qualToErrorProbRaw(i); + for (int i = 0; i < 256; i++) qualToErrorProbCache[i] = qualToErrorProbRaw((byte)i); } /** From d471617c65f6d1f3885ea6628b7676ed6bbc6f8d Mon Sep 17 00:00:00 2001 From: Mark DePristo Date: Fri, 2 Sep 2011 21:15:19 -0400 Subject: [PATCH 07/41] GATK binary VCF (gvcf) prototype format for efficiency testing -- Very minimal working version that can read / write binary VCFs with genotypes -- Already 10x faster for sites, 5x for fully parsed genotypes, and 1000x for skipping genotypes when reading --- .../broadinstitute/sting/utils/gvcf/GVCF.java | 252 ++++++++++++++++++ .../sting/utils/gvcf/GVCFGenotype.java | 147 ++++++++++ .../sting/utils/gvcf/GVCFHeader.java | 180 +++++++++++++ .../sting/utils/gvcf/GVCFHeaderBuilder.java | 80 ++++++ 4 files changed, 659 insertions(+) create mode 100644 public/java/src/org/broadinstitute/sting/utils/gvcf/GVCF.java create mode 100644 public/java/src/org/broadinstitute/sting/utils/gvcf/GVCFGenotype.java create mode 100644 public/java/src/org/broadinstitute/sting/utils/gvcf/GVCFHeader.java create mode 100644 public/java/src/org/broadinstitute/sting/utils/gvcf/GVCFHeaderBuilder.java diff --git a/public/java/src/org/broadinstitute/sting/utils/gvcf/GVCF.java b/public/java/src/org/broadinstitute/sting/utils/gvcf/GVCF.java new file mode 100644 index 000000000..8568c1aab --- /dev/null +++ b/public/java/src/org/broadinstitute/sting/utils/gvcf/GVCF.java @@ -0,0 +1,252 @@ +/* + * Copyright (c) 2011, The Broad Institute + * + * Permission is hereby granted, free of charge, to any person + * obtaining a copy of this software and associated documentation + * files (the "Software"), to deal in the Software without + * restriction, including without limitation the rights to use, + * copy, modify, merge, publish, distribute, sublicense, and/or sell + * copies of the Software, and to permit persons to whom the + * Software is furnished to do so, subject to the following + * conditions: + * + * The above copyright notice and this permission notice shall be + * included in all copies or substantial portions of the Software. + * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, + * EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES + * OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND + * NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT + * HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, + * WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING + * FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR + * OTHER DEALINGS IN THE SOFTWARE. + */ + +package org.broadinstitute.sting.utils.gvcf; + +import org.broadinstitute.sting.utils.QualityUtils; +import org.broadinstitute.sting.utils.Utils; +import org.broadinstitute.sting.utils.codecs.vcf.StandardVCFWriter; +import org.broadinstitute.sting.utils.codecs.vcf.VCFConstants; +import org.broadinstitute.sting.utils.exceptions.UserException; +import org.broadinstitute.sting.utils.variantcontext.Allele; +import org.broadinstitute.sting.utils.variantcontext.Genotype; +import org.broadinstitute.sting.utils.variantcontext.VariantContext; + +import java.io.*; +import java.util.*; + +/** + * GATK binary VCF record + * + * @author Your Name + * @since Date created + */ +public class GVCF { + private final static int RECORD_TERMINATOR = 123456789; + private int chromOffset; + private int start, stop; + private String id; + private List alleleMap; + private int alleleOffsets[]; + private float qual; + private byte refPad; + private String info; + private int filterOffset; + + private List genotypes = Collections.emptyList(); + + public GVCF(final GVCFHeaderBuilder gvcfHeaderBuilder, final VariantContext vc, boolean skipGenotypes) { + chromOffset = gvcfHeaderBuilder.encodeString(vc.getChr()); + start = vc.getStart(); + stop = vc.getEnd(); + refPad = vc.hasReferenceBaseForIndel() ? vc.getReferenceBaseForIndel() : 0; + id = vc.getID(); + + // encode alleles + alleleMap = new ArrayList(vc.getNAlleles()); + alleleOffsets = new int[vc.getNAlleles()]; + alleleMap.add(vc.getReference()); + alleleOffsets[0] = gvcfHeaderBuilder.encodeAllele(vc.getReference()); + for ( int i = 0; i < vc.getAlternateAlleles().size(); i++ ) { + alleleMap.add(vc.getAlternateAllele(i)); + alleleOffsets[i+1] = gvcfHeaderBuilder.encodeAllele(vc.getAlternateAllele(i)); + } + + qual = (float)vc.getNegLog10PError(); //qualToByte(vc.getPhredScaledQual()); + info = infoFieldString(vc, gvcfHeaderBuilder); + filterOffset = gvcfHeaderBuilder.encodeString(StandardVCFWriter.getFilterString(vc)); + + if ( ! skipGenotypes ) { + genotypes = encodeGenotypes(gvcfHeaderBuilder, vc); + } + } + + public GVCF(DataInputStream inputStream, boolean skipGenotypes) throws IOException { + chromOffset = inputStream.readInt(); + start = inputStream.readInt(); + stop = inputStream.readInt(); + id = inputStream.readUTF(); + refPad = inputStream.readByte(); + alleleOffsets = readIntArray(inputStream); + qual = inputStream.readFloat(); + info = inputStream.readUTF(); + filterOffset = inputStream.readInt(); + + int nGenotypes = inputStream.readInt(); + int sizeOfGenotypes = inputStream.readInt(); + if ( skipGenotypes ) { + genotypes = Collections.emptyList(); + inputStream.skipBytes(sizeOfGenotypes); + } else { + genotypes = new ArrayList(nGenotypes); + for ( int i = 0; i < nGenotypes; i++ ) + genotypes.add(new GVCFGenotype(this, inputStream)); + } + + int recordDone = inputStream.readInt(); + if ( recordDone != RECORD_TERMINATOR ) + throw new UserException.MalformedFile("Record not terminated by RECORD_TERMINATOR key"); + } + + public VariantContext decode(final String source, final GVCFHeader header) { + final String contig = header.getString(chromOffset); + alleleMap = header.getAlleles(alleleOffsets); + double negLog10PError = qual; // QualityUtils.qualToErrorProb(qual); + Set filters = header.getFilters(filterOffset); + Map attributes = new HashMap(); + attributes.put("INFO", info); + Byte refPadByte = refPad == 0 ? null : refPad; + Map genotypes = decodeGenotypes(header); + + return new VariantContext(source, contig, start, stop, alleleMap, genotypes, negLog10PError, filters, attributes, refPadByte); + } + + private Map decodeGenotypes(final GVCFHeader header) { + if ( genotypes.isEmpty() ) + return VariantContext.NO_GENOTYPES; + else { + Map map = new TreeMap(); + + for ( int i = 0; i < genotypes.size(); i++ ) { + final String sampleName = header.getSample(i); + final Genotype g = genotypes.get(i).decode(sampleName, header, this, alleleMap); + map.put(sampleName, g); + } + + return map; + } + } + + private List encodeGenotypes(final GVCFHeaderBuilder gvcfHeaderBuilder, final VariantContext vc) { + int nGenotypes = vc.getNSamples(); + if ( nGenotypes > 0 ) { + List genotypes = new ArrayList(nGenotypes); + for ( int i = 0; i < nGenotypes; i++ ) genotypes.add(null); + + for ( Genotype g : vc.getGenotypes().values() ) { + int i = gvcfHeaderBuilder.encodeSample(g.getSampleName()); + genotypes.set(i, new GVCFGenotype(gvcfHeaderBuilder, alleleMap, g)); + } + + return genotypes; + } else { + return Collections.emptyList(); + } + } + + public int getNAlleles() { return alleleOffsets.length; } + + public int write(DataOutputStream outputStream) throws IOException { + int startSize = outputStream.size(); + outputStream.writeInt(chromOffset); + outputStream.writeInt(start); + outputStream.writeInt(stop); + outputStream.writeUTF(id); + outputStream.writeByte(refPad); + writeIntArray(alleleOffsets, outputStream, true); + outputStream.writeFloat(qual); + outputStream.writeUTF(info); + outputStream.writeInt(filterOffset); + + int nGenotypes = genotypes.size(); + int expectedSizeOfGenotypes = nGenotypes == 0 ? 0 : genotypes.get(0).sizeInBytes() * nGenotypes; + outputStream.writeInt(nGenotypes); + outputStream.writeInt(expectedSizeOfGenotypes); + int obsSizeOfGenotypes = 0; + for ( GVCFGenotype g : genotypes ) + obsSizeOfGenotypes += g.write(outputStream); + if ( obsSizeOfGenotypes != expectedSizeOfGenotypes ) + throw new RuntimeException("Expect and observed genotype sizes disagree! expect = " + expectedSizeOfGenotypes + " obs =" + obsSizeOfGenotypes); + + outputStream.writeInt(RECORD_TERMINATOR); + return outputStream.size() - startSize; + } + + private final String infoFieldString(VariantContext vc, final GVCFHeaderBuilder gvcfHeaderBuilder) { + StringBuilder s = new StringBuilder(); + + boolean first = true; + for ( Map.Entry field : vc.getAttributes().entrySet() ) { + String key = field.getKey(); + if ( key.equals(VariantContext.ID_KEY) || key.equals(VariantContext.UNPARSED_GENOTYPE_MAP_KEY) || key.equals(VariantContext.UNPARSED_GENOTYPE_PARSER_KEY) ) + continue; + int stringIndex = gvcfHeaderBuilder.encodeString(key); + String outputValue = StandardVCFWriter.formatVCFField(field.getValue()); + if ( outputValue != null ) { + if ( ! first ) s.append(";"); + s.append(stringIndex).append("=").append(outputValue); + first = false; + } + } + + return s.toString(); + } + + private final static int BUFFER_SIZE = 1048576; // 2**20 + public static DataOutputStream createOutputStream(final File file) throws FileNotFoundException { + return new DataOutputStream(new BufferedOutputStream(new FileOutputStream(file), BUFFER_SIZE)); + } + + public static DataInputStream createInputStream(final File file) throws FileNotFoundException { + return new DataInputStream(new BufferedInputStream(new FileInputStream(file), BUFFER_SIZE)); + } + + protected final static int[] readIntArray(final DataInputStream inputStream) throws IOException { + return readIntArray(inputStream, inputStream.readInt()); + } + + protected final static int[] readIntArray(final DataInputStream inputStream, int size) throws IOException { + int[] array = new int[size]; + for ( int i = 0; i < array.length; i++ ) + array[i] = inputStream.readInt(); + return array; + } + + protected final static void writeIntArray(int[] array, final DataOutputStream outputStream, boolean writeSize) throws IOException { + if ( writeSize ) outputStream.writeInt(array.length); + for ( int i : array ) + outputStream.writeInt(i); + } + + protected final static byte[] readByteArray(final DataInputStream inputStream) throws IOException { + return readByteArray(inputStream, inputStream.readInt()); + } + + protected final static byte[] readByteArray(final DataInputStream inputStream, int size) throws IOException { + byte[] array = new byte[size]; + for ( int i = 0; i < array.length; i++ ) + array[i] = inputStream.readByte(); + return array; + } + + protected final static void writeByteArray(byte[] array, final DataOutputStream outputStream, boolean writeSize) throws IOException { + if ( writeSize ) outputStream.writeInt(array.length); + for ( byte i : array ) + outputStream.writeByte(i); + } + + protected final static byte qualToByte(double phredScaledQual) { + return (byte)Math.round(Math.min(phredScaledQual, 255)); + } +} diff --git a/public/java/src/org/broadinstitute/sting/utils/gvcf/GVCFGenotype.java b/public/java/src/org/broadinstitute/sting/utils/gvcf/GVCFGenotype.java new file mode 100644 index 000000000..2ef6d9b3a --- /dev/null +++ b/public/java/src/org/broadinstitute/sting/utils/gvcf/GVCFGenotype.java @@ -0,0 +1,147 @@ +/* + * Copyright (c) 2011, The Broad Institute + * + * Permission is hereby granted, free of charge, to any person + * obtaining a copy of this software and associated documentation + * files (the "Software"), to deal in the Software without + * restriction, including without limitation the rights to use, + * copy, modify, merge, publish, distribute, sublicense, and/or sell + * copies of the Software, and to permit persons to whom the + * Software is furnished to do so, subject to the following + * conditions: + * + * The above copyright notice and this permission notice shall be + * included in all copies or substantial portions of the Software. + * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, + * EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES + * OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND + * NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT + * HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, + * WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING + * FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR + * OTHER DEALINGS IN THE SOFTWARE. + */ + +package org.broadinstitute.sting.utils.gvcf; + +import org.broadinstitute.sting.utils.variantcontext.Allele; +import org.broadinstitute.sting.utils.variantcontext.Genotype; + +import java.io.DataInputStream; +import java.io.DataOutputStream; +import java.io.IOException; +import java.util.*; + +/** + * GATK binary VCF record + * + * @author Your Name + * @since Date created + */ +public class GVCFGenotype { + private byte gq; + private int gt; + private int dp; + private int ad[]; + private byte[] pl; + + // todo -- what to do about phasing? Perhaps we shouldn't support it + // todo -- is the FL field generic or just a flag? Should we even support per sample filtering? + + public GVCFGenotype(final GVCFHeaderBuilder gvcfHeaderBuilder, final List allAlleles, Genotype genotype) { + gq = GVCF.qualToByte(genotype.getPhredScaledQual()); + gt = encodeAlleles(genotype.getAlleles(), allAlleles); + + dp = genotype.getAttributeAsInt("DP", 0); + + int nAlleles = allAlleles.size(); + ad = new int[nAlleles]; + + int npls = nAllelesToNPls(nAlleles); + pl = new byte[npls]; + } + + private int nAllelesToNPls( int nAlleles ) { + return nAlleles*(nAlleles+1) / 2; + } + + public GVCFGenotype(GVCF gvcf, DataInputStream inputStream) throws IOException { + int gqInt = inputStream.readUnsignedByte(); + gq = (byte)gqInt; + gt = inputStream.readInt(); + dp = inputStream.readInt(); + ad = GVCF.readIntArray(inputStream, gvcf.getNAlleles()); + pl = GVCF.readByteArray(inputStream, nAllelesToNPls(gvcf.getNAlleles())); + } + + // 2 alleles => 1 + 8 + 8 + 3 => 20 + protected int sizeInBytes() { + return 1 // gq + + 4 * 2 // gt + dp + + 4 * ad.length // ad + + 1 * pl.length; // pl + } + + public Genotype decode(final String sampleName, final GVCFHeader header, GVCF gvcf, List alleleIndex) { + final List alleles = decodeAlleles(gt, alleleIndex); + final double negLog10PError = gq / 10.0; + final Set filters = Collections.emptySet(); + final Map attributes = new HashMap(); + attributes.put("DP", dp); + attributes.put("AD", ad); + attributes.put("PL", pl); + + return new Genotype(sampleName, alleles, negLog10PError, filters, attributes, false); + } + + private static int encodeAlleles(List gtList, List allAlleles) { + final int nAlleles = gtList.size(); + if ( nAlleles > 4 ) + throw new IllegalArgumentException("encodeAlleles doesn't support more than 4 alt alleles, but I saw " + gtList); + + int gtInt = 0; + for ( int i = 0; i < nAlleles ; i++ ) { + final int bitOffset = i * 8; + final int allelei = getAlleleIndex(gtList.get(i), allAlleles); + final int gti = (allelei + 1) << bitOffset; + gtInt = gtInt | gti; + } + + return gtInt; + } + + private static int getAlleleIndex(Allele q, List allAlleles) { + if ( q.isNoCall() ) + return 254; + for ( int i = 0; i < allAlleles.size(); i++ ) + if ( q.equals(allAlleles.get(i)) ) + return i; + throw new IllegalStateException("getAlleleIndex passed allele not in map! allele " + q + " allAlleles " + allAlleles); + } + + private static List decodeAlleles(int gtInt, List alleleIndex) { + List alleles = new ArrayList(4); + + for ( int i = 0; i < 32; i += 8 ) { + final int gi = (gtInt & (0x000000FF << i)) >> i; + if ( gi != 0 ) { + final int allelei = gi - 1; + alleles.add( allelei == 254 ? Allele.NO_CALL : alleleIndex.get(allelei) ); + } else { + break; + } + } + + return alleles; + } + + public int write(DataOutputStream outputStream) throws IOException { + int startSize = outputStream.size(); + outputStream.writeByte(gq); + outputStream.writeInt(gt); + outputStream.writeInt(dp); + GVCF.writeIntArray(ad, outputStream, false); + GVCF.writeByteArray(pl, outputStream, false); + return outputStream.size() - startSize; + } +} diff --git a/public/java/src/org/broadinstitute/sting/utils/gvcf/GVCFHeader.java b/public/java/src/org/broadinstitute/sting/utils/gvcf/GVCFHeader.java new file mode 100644 index 000000000..c52c975bd --- /dev/null +++ b/public/java/src/org/broadinstitute/sting/utils/gvcf/GVCFHeader.java @@ -0,0 +1,180 @@ +/* + * Copyright (c) 2011, The Broad Institute + * + * Permission is hereby granted, free of charge, to any person + * obtaining a copy of this software and associated documentation + * files (the "Software"), to deal in the Software without + * restriction, including without limitation the rights to use, + * copy, modify, merge, publish, distribute, sublicense, and/or sell + * copies of the Software, and to permit persons to whom the + * Software is furnished to do so, subject to the following + * conditions: + * + * The above copyright notice and this permission notice shall be + * included in all copies or substantial portions of the Software. + * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, + * EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES + * OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND + * NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT + * HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, + * WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING + * FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR + * OTHER DEALINGS IN THE SOFTWARE. + */ + +package org.broadinstitute.sting.utils.gvcf; + +import org.apache.log4j.Logger; +import org.broadinstitute.sting.utils.QualityUtils; +import org.broadinstitute.sting.utils.codecs.vcf.AbstractVCFCodec; +import org.broadinstitute.sting.utils.codecs.vcf.VCFCodec; +import org.broadinstitute.sting.utils.codecs.vcf.VCFConstants; +import org.broadinstitute.sting.utils.exceptions.UserException; +import org.broadinstitute.sting.utils.variantcontext.Allele; + +import java.io.DataInputStream; +import java.io.DataOutputStream; +import java.io.IOException; +import java.util.*; + +/** + * [Short one sentence description of this walker] + *

+ *

+ * [Functionality of this walker] + *

+ *

+ *

Input

+ *

+ * [Input description] + *

+ *

+ *

Output

+ *

+ * [Output description] + *

+ *

+ *

Examples

+ * 
+ *    java
+ *      -jar GenomeAnalysisTK.jar
+ *      -T $WalkerName
+ *
+ * + * @author Your Name + * @since Date created + */ +public class GVCFHeader { + final protected static Logger logger = Logger.getLogger(GVCFHeader.class); + + private static byte[] MAGIC_HEADER = "GVCF0.1\1".getBytes(); + final List alleles; + final List strings; + final List samples; + final List> filters; + + public GVCFHeader(final Map allelesIn, final Map stringIn, final Map samplesIn) { + this.alleles = linearize(allelesIn); + this.strings = linearize(stringIn); + this.samples = linearize(samplesIn); + this.filters = null; // not used with this constructor + } + + public GVCFHeader(DataInputStream inputStream) throws IOException { + byte[] headerTest = new byte[MAGIC_HEADER.length]; + inputStream.read(headerTest); + if ( ! Arrays.equals(headerTest, MAGIC_HEADER) ) { + throw new UserException("Could not read GVCF file. MAGIC_HEADER missing. Saw " + headerTest); + } else { + alleles = stringsToAlleles(readStrings(inputStream)); + strings = readStrings(inputStream); + samples = readStrings(inputStream); + logger.info(String.format("Allele map of %d elements", alleles.size())); + logger.info(String.format("String map of %d elements", strings.size())); + logger.info(String.format("Sample map of %d elements", samples.size())); + filters = initializeFilterCache(); + } + } + + public int write(final DataOutputStream outputStream) throws IOException { + int startBytes = outputStream.size(); + outputStream.write(MAGIC_HEADER); + write(outputStream, allelesToStrings(alleles)); + write(outputStream, strings); + write(outputStream, samples); + return outputStream.size() - startBytes; + } + + public void write(DataOutputStream outputStream, List l) throws IOException { + outputStream.writeInt(l.size()); + for ( String elt : l ) outputStream.writeUTF(elt); + } + + private List allelesToStrings(List alleles) { + List strings = new ArrayList(alleles.size()); + for ( Allele allele : alleles ) strings.add(allele.toString()); + return strings; + } + + private List> initializeFilterCache() { + // required to allow offset -> set lookup + List> l = new ArrayList>(strings.size()); + for ( int i = 0; i < strings.size(); i++ ) l.add(null); + return l; + } + + private static List stringsToAlleles(final List strings) { + final List alleles = new ArrayList(strings.size()); + for ( String string : strings ) { + boolean isRef = string.endsWith("*"); + if ( isRef ) string = string.substring(0, string.length() - 1); + alleles.add(Allele.create(string, isRef)); + } + return alleles; + } + + private static List readStrings(final DataInputStream inputStream) throws IOException { + final int nStrings = inputStream.readInt(); + + final List strings = new ArrayList(nStrings); + for ( int i = 0; i < nStrings; i++ ) { + strings.add(inputStream.readUTF()); + } + + return strings; + } + + private static List linearize(final Map map) { + final ArrayList l = new ArrayList(map.size()); + for ( int i = 0; i < map.size(); i++ ) l.add(null); + for ( final Map.Entry elt : map.entrySet() ) + l.set(elt.getValue(), elt.getKey()); + return l; + } + + public String getSample(final int offset) { return samples.get(offset); } + public String getString(final int offset) { return strings.get(offset); } + public Allele getAllele(final int offset) { return alleles.get(offset); } + public List getAlleles(final int[] offsets) { + final List alleles = new ArrayList(offsets.length); + for ( int i : offsets ) alleles.add(getAllele(i)); + return alleles; + } + + public Set getFilters(final int offset) { + Set cached = filters.get(offset); + + if ( cached != null ) + return cached; + else { + final String filterString = getString(offset); + if ( filterString.equals(VCFConstants.UNFILTERED) ) + return null; // UNFILTERED records are represented by null + else { + Set set = VCFCodec.parseFilters(null, -1, filterString); + filters.set(offset, set); // remember the result + return set; + } + } + } +} diff --git a/public/java/src/org/broadinstitute/sting/utils/gvcf/GVCFHeaderBuilder.java b/public/java/src/org/broadinstitute/sting/utils/gvcf/GVCFHeaderBuilder.java new file mode 100644 index 000000000..2d045b8ea --- /dev/null +++ b/public/java/src/org/broadinstitute/sting/utils/gvcf/GVCFHeaderBuilder.java @@ -0,0 +1,80 @@ +/* + * Copyright (c) 2011, The Broad Institute + * + * Permission is hereby granted, free of charge, to any person + * obtaining a copy of this software and associated documentation + * files (the "Software"), to deal in the Software without + * restriction, including without limitation the rights to use, + * copy, modify, merge, publish, distribute, sublicense, and/or sell + * copies of the Software, and to permit persons to whom the + * Software is furnished to do so, subject to the following + * conditions: + * + * The above copyright notice and this permission notice shall be + * included in all copies or substantial portions of the Software. + * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, + * EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES + * OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND + * NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT + * HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, + * WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING + * FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR + * OTHER DEALINGS IN THE SOFTWARE. + */ + +package org.broadinstitute.sting.utils.gvcf; + +import org.broadinstitute.sting.utils.variantcontext.Allele; + +import java.util.HashMap; +import java.util.Map; + +/** + * [Short one sentence description of this walker] + *

+ *

+ * [Functionality of this walker] + *

+ *

+ *

Input

+ *

+ * [Input description] + *

+ *

+ *

Output

+ *

+ * [Output description] + *

+ *

+ *

Examples

+ * 
+ *    java
+ *      -jar GenomeAnalysisTK.jar
+ *      -T $WalkerName
+ *
+ * + * @author Your Name + * @since Date created + */ +public class GVCFHeaderBuilder { + Map alleles = new HashMap(); + Map strings = new HashMap(); + Map samples = new HashMap(); + + public GVCFHeader createHeader() { + return new GVCFHeader(alleles, strings, samples); + } + + public int encodeString(final String chr) { return encode(strings, chr); } + public int encodeAllele(final Allele allele) { return encode(alleles, allele); } + public int encodeSample(final String sampleName) { return encode(samples, sampleName); } + + private int encode(Map map, T key) { + Integer v = map.get(key); + if ( v == null ) { + v = map.size(); + map.put(key, v); + } + return v; + } +} From 01b6177ce15c2d4270ac863da1a2e4e43e020411 Mon Sep 17 00:00:00 2001 From: Mark DePristo Date: Wed, 7 Sep 2011 17:10:56 -0400 Subject: [PATCH 08/41] Renaming GVCF -> GCF --- .../utils/{gvcf/GVCF.java => gcf/GCF.java} | 47 +++++++++---------- .../GCFGenotype.java} | 20 ++++---- .../GVCFHeader.java => gcf/GCFHeader.java} | 12 ++--- .../GCFHeaderBuilder.java} | 8 ++-- 4 files changed, 41 insertions(+), 46 deletions(-) rename public/java/src/org/broadinstitute/sting/utils/{gvcf/GVCF.java => gcf/GCF.java} (83%) rename public/java/src/org/broadinstitute/sting/utils/{gvcf/GVCFGenotype.java => gcf/GCFGenotype.java} (86%) rename public/java/src/org/broadinstitute/sting/utils/{gvcf/GVCFHeader.java => gcf/GCFHeader.java} (92%) rename public/java/src/org/broadinstitute/sting/utils/{gvcf/GVCFHeaderBuilder.java => gcf/GCFHeaderBuilder.java} (93%) diff --git a/public/java/src/org/broadinstitute/sting/utils/gvcf/GVCF.java b/public/java/src/org/broadinstitute/sting/utils/gcf/GCF.java similarity index 83% rename from public/java/src/org/broadinstitute/sting/utils/gvcf/GVCF.java rename to public/java/src/org/broadinstitute/sting/utils/gcf/GCF.java index 8568c1aab..5ab241ebf 100644 --- a/public/java/src/org/broadinstitute/sting/utils/gvcf/GVCF.java +++ b/public/java/src/org/broadinstitute/sting/utils/gcf/GCF.java @@ -22,12 +22,9 @@ * OTHER DEALINGS IN THE SOFTWARE. */ -package org.broadinstitute.sting.utils.gvcf; +package org.broadinstitute.sting.utils.gcf; -import org.broadinstitute.sting.utils.QualityUtils; -import org.broadinstitute.sting.utils.Utils; import org.broadinstitute.sting.utils.codecs.vcf.StandardVCFWriter; -import org.broadinstitute.sting.utils.codecs.vcf.VCFConstants; import org.broadinstitute.sting.utils.exceptions.UserException; import org.broadinstitute.sting.utils.variantcontext.Allele; import org.broadinstitute.sting.utils.variantcontext.Genotype; @@ -42,7 +39,7 @@ import java.util.*; * @author Your Name * @since Date created */ -public class GVCF { +public class GCF { private final static int RECORD_TERMINATOR = 123456789; private int chromOffset; private int start, stop; @@ -54,10 +51,10 @@ public class GVCF { private String info; private int filterOffset; - private List genotypes = Collections.emptyList(); + private List genotypes = Collections.emptyList(); - public GVCF(final GVCFHeaderBuilder gvcfHeaderBuilder, final VariantContext vc, boolean skipGenotypes) { - chromOffset = gvcfHeaderBuilder.encodeString(vc.getChr()); + public GCF(final GCFHeaderBuilder GCFHeaderBuilder, final VariantContext vc, boolean skipGenotypes) { + chromOffset = GCFHeaderBuilder.encodeString(vc.getChr()); start = vc.getStart(); stop = vc.getEnd(); refPad = vc.hasReferenceBaseForIndel() ? vc.getReferenceBaseForIndel() : 0; @@ -67,22 +64,22 @@ public class GVCF { alleleMap = new ArrayList(vc.getNAlleles()); alleleOffsets = new int[vc.getNAlleles()]; alleleMap.add(vc.getReference()); - alleleOffsets[0] = gvcfHeaderBuilder.encodeAllele(vc.getReference()); + alleleOffsets[0] = GCFHeaderBuilder.encodeAllele(vc.getReference()); for ( int i = 0; i < vc.getAlternateAlleles().size(); i++ ) { alleleMap.add(vc.getAlternateAllele(i)); - alleleOffsets[i+1] = gvcfHeaderBuilder.encodeAllele(vc.getAlternateAllele(i)); + alleleOffsets[i+1] = GCFHeaderBuilder.encodeAllele(vc.getAlternateAllele(i)); } qual = (float)vc.getNegLog10PError(); //qualToByte(vc.getPhredScaledQual()); - info = infoFieldString(vc, gvcfHeaderBuilder); - filterOffset = gvcfHeaderBuilder.encodeString(StandardVCFWriter.getFilterString(vc)); + info = infoFieldString(vc, GCFHeaderBuilder); + filterOffset = GCFHeaderBuilder.encodeString(StandardVCFWriter.getFilterString(vc)); if ( ! skipGenotypes ) { - genotypes = encodeGenotypes(gvcfHeaderBuilder, vc); + genotypes = encodeGenotypes(GCFHeaderBuilder, vc); } } - public GVCF(DataInputStream inputStream, boolean skipGenotypes) throws IOException { + public GCF(DataInputStream inputStream, boolean skipGenotypes) throws IOException { chromOffset = inputStream.readInt(); start = inputStream.readInt(); stop = inputStream.readInt(); @@ -99,9 +96,9 @@ public class GVCF { genotypes = Collections.emptyList(); inputStream.skipBytes(sizeOfGenotypes); } else { - genotypes = new ArrayList(nGenotypes); + genotypes = new ArrayList(nGenotypes); for ( int i = 0; i < nGenotypes; i++ ) - genotypes.add(new GVCFGenotype(this, inputStream)); + genotypes.add(new GCFGenotype(this, inputStream)); } int recordDone = inputStream.readInt(); @@ -109,7 +106,7 @@ public class GVCF { throw new UserException.MalformedFile("Record not terminated by RECORD_TERMINATOR key"); } - public VariantContext decode(final String source, final GVCFHeader header) { + public VariantContext decode(final String source, final GCFHeader header) { final String contig = header.getString(chromOffset); alleleMap = header.getAlleles(alleleOffsets); double negLog10PError = qual; // QualityUtils.qualToErrorProb(qual); @@ -122,7 +119,7 @@ public class GVCF { return new VariantContext(source, contig, start, stop, alleleMap, genotypes, negLog10PError, filters, attributes, refPadByte); } - private Map decodeGenotypes(final GVCFHeader header) { + private Map decodeGenotypes(final GCFHeader header) { if ( genotypes.isEmpty() ) return VariantContext.NO_GENOTYPES; else { @@ -138,15 +135,15 @@ public class GVCF { } } - private List encodeGenotypes(final GVCFHeaderBuilder gvcfHeaderBuilder, final VariantContext vc) { + private List encodeGenotypes(final GCFHeaderBuilder GCFHeaderBuilder, final VariantContext vc) { int nGenotypes = vc.getNSamples(); if ( nGenotypes > 0 ) { - List genotypes = new ArrayList(nGenotypes); + List genotypes = new ArrayList(nGenotypes); for ( int i = 0; i < nGenotypes; i++ ) genotypes.add(null); for ( Genotype g : vc.getGenotypes().values() ) { - int i = gvcfHeaderBuilder.encodeSample(g.getSampleName()); - genotypes.set(i, new GVCFGenotype(gvcfHeaderBuilder, alleleMap, g)); + int i = GCFHeaderBuilder.encodeSample(g.getSampleName()); + genotypes.set(i, new GCFGenotype(GCFHeaderBuilder, alleleMap, g)); } return genotypes; @@ -174,7 +171,7 @@ public class GVCF { outputStream.writeInt(nGenotypes); outputStream.writeInt(expectedSizeOfGenotypes); int obsSizeOfGenotypes = 0; - for ( GVCFGenotype g : genotypes ) + for ( GCFGenotype g : genotypes ) obsSizeOfGenotypes += g.write(outputStream); if ( obsSizeOfGenotypes != expectedSizeOfGenotypes ) throw new RuntimeException("Expect and observed genotype sizes disagree! expect = " + expectedSizeOfGenotypes + " obs =" + obsSizeOfGenotypes); @@ -183,7 +180,7 @@ public class GVCF { return outputStream.size() - startSize; } - private final String infoFieldString(VariantContext vc, final GVCFHeaderBuilder gvcfHeaderBuilder) { + private final String infoFieldString(VariantContext vc, final GCFHeaderBuilder GCFHeaderBuilder) { StringBuilder s = new StringBuilder(); boolean first = true; @@ -191,7 +188,7 @@ public class GVCF { String key = field.getKey(); if ( key.equals(VariantContext.ID_KEY) || key.equals(VariantContext.UNPARSED_GENOTYPE_MAP_KEY) || key.equals(VariantContext.UNPARSED_GENOTYPE_PARSER_KEY) ) continue; - int stringIndex = gvcfHeaderBuilder.encodeString(key); + int stringIndex = GCFHeaderBuilder.encodeString(key); String outputValue = StandardVCFWriter.formatVCFField(field.getValue()); if ( outputValue != null ) { if ( ! first ) s.append(";"); diff --git a/public/java/src/org/broadinstitute/sting/utils/gvcf/GVCFGenotype.java b/public/java/src/org/broadinstitute/sting/utils/gcf/GCFGenotype.java similarity index 86% rename from public/java/src/org/broadinstitute/sting/utils/gvcf/GVCFGenotype.java rename to public/java/src/org/broadinstitute/sting/utils/gcf/GCFGenotype.java index 2ef6d9b3a..dd1fb091c 100644 --- a/public/java/src/org/broadinstitute/sting/utils/gvcf/GVCFGenotype.java +++ b/public/java/src/org/broadinstitute/sting/utils/gcf/GCFGenotype.java @@ -22,7 +22,7 @@ * OTHER DEALINGS IN THE SOFTWARE. */ -package org.broadinstitute.sting.utils.gvcf; +package org.broadinstitute.sting.utils.gcf; import org.broadinstitute.sting.utils.variantcontext.Allele; import org.broadinstitute.sting.utils.variantcontext.Genotype; @@ -38,7 +38,7 @@ import java.util.*; * @author Your Name * @since Date created */ -public class GVCFGenotype { +public class GCFGenotype { private byte gq; private int gt; private int dp; @@ -48,8 +48,8 @@ public class GVCFGenotype { // todo -- what to do about phasing? Perhaps we shouldn't support it // todo -- is the FL field generic or just a flag? Should we even support per sample filtering? - public GVCFGenotype(final GVCFHeaderBuilder gvcfHeaderBuilder, final List allAlleles, Genotype genotype) { - gq = GVCF.qualToByte(genotype.getPhredScaledQual()); + public GCFGenotype(final GCFHeaderBuilder GCFHeaderBuilder, final List allAlleles, Genotype genotype) { + gq = GCF.qualToByte(genotype.getPhredScaledQual()); gt = encodeAlleles(genotype.getAlleles(), allAlleles); dp = genotype.getAttributeAsInt("DP", 0); @@ -65,13 +65,13 @@ public class GVCFGenotype { return nAlleles*(nAlleles+1) / 2; } - public GVCFGenotype(GVCF gvcf, DataInputStream inputStream) throws IOException { + public GCFGenotype(GCF GCF, DataInputStream inputStream) throws IOException { int gqInt = inputStream.readUnsignedByte(); gq = (byte)gqInt; gt = inputStream.readInt(); dp = inputStream.readInt(); - ad = GVCF.readIntArray(inputStream, gvcf.getNAlleles()); - pl = GVCF.readByteArray(inputStream, nAllelesToNPls(gvcf.getNAlleles())); + ad = GCF.readIntArray(inputStream, GCF.getNAlleles()); + pl = GCF.readByteArray(inputStream, nAllelesToNPls(GCF.getNAlleles())); } // 2 alleles => 1 + 8 + 8 + 3 => 20 @@ -82,7 +82,7 @@ public class GVCFGenotype { + 1 * pl.length; // pl } - public Genotype decode(final String sampleName, final GVCFHeader header, GVCF gvcf, List alleleIndex) { + public Genotype decode(final String sampleName, final GCFHeader header, GCF GCF, List alleleIndex) { final List alleles = decodeAlleles(gt, alleleIndex); final double negLog10PError = gq / 10.0; final Set filters = Collections.emptySet(); @@ -140,8 +140,8 @@ public class GVCFGenotype { outputStream.writeByte(gq); outputStream.writeInt(gt); outputStream.writeInt(dp); - GVCF.writeIntArray(ad, outputStream, false); - GVCF.writeByteArray(pl, outputStream, false); + GCF.writeIntArray(ad, outputStream, false); + GCF.writeByteArray(pl, outputStream, false); return outputStream.size() - startSize; } } diff --git a/public/java/src/org/broadinstitute/sting/utils/gvcf/GVCFHeader.java b/public/java/src/org/broadinstitute/sting/utils/gcf/GCFHeader.java similarity index 92% rename from public/java/src/org/broadinstitute/sting/utils/gvcf/GVCFHeader.java rename to public/java/src/org/broadinstitute/sting/utils/gcf/GCFHeader.java index c52c975bd..d0c765cc4 100644 --- a/public/java/src/org/broadinstitute/sting/utils/gvcf/GVCFHeader.java +++ b/public/java/src/org/broadinstitute/sting/utils/gcf/GCFHeader.java @@ -22,11 +22,9 @@ * OTHER DEALINGS IN THE SOFTWARE. */ -package org.broadinstitute.sting.utils.gvcf; +package org.broadinstitute.sting.utils.gcf; import org.apache.log4j.Logger; -import org.broadinstitute.sting.utils.QualityUtils; -import org.broadinstitute.sting.utils.codecs.vcf.AbstractVCFCodec; import org.broadinstitute.sting.utils.codecs.vcf.VCFCodec; import org.broadinstitute.sting.utils.codecs.vcf.VCFConstants; import org.broadinstitute.sting.utils.exceptions.UserException; @@ -64,8 +62,8 @@ import java.util.*; * @author Your Name * @since Date created */ -public class GVCFHeader { - final protected static Logger logger = Logger.getLogger(GVCFHeader.class); +public class GCFHeader { + final protected static Logger logger = Logger.getLogger(GCFHeader.class); private static byte[] MAGIC_HEADER = "GVCF0.1\1".getBytes(); final List alleles; @@ -73,14 +71,14 @@ public class GVCFHeader { final List samples; final List> filters; - public GVCFHeader(final Map allelesIn, final Map stringIn, final Map samplesIn) { + public GCFHeader(final Map allelesIn, final Map stringIn, final Map samplesIn) { this.alleles = linearize(allelesIn); this.strings = linearize(stringIn); this.samples = linearize(samplesIn); this.filters = null; // not used with this constructor } - public GVCFHeader(DataInputStream inputStream) throws IOException { + public GCFHeader(DataInputStream inputStream) throws IOException { byte[] headerTest = new byte[MAGIC_HEADER.length]; inputStream.read(headerTest); if ( ! Arrays.equals(headerTest, MAGIC_HEADER) ) { diff --git a/public/java/src/org/broadinstitute/sting/utils/gvcf/GVCFHeaderBuilder.java b/public/java/src/org/broadinstitute/sting/utils/gcf/GCFHeaderBuilder.java similarity index 93% rename from public/java/src/org/broadinstitute/sting/utils/gvcf/GVCFHeaderBuilder.java rename to public/java/src/org/broadinstitute/sting/utils/gcf/GCFHeaderBuilder.java index 2d045b8ea..40e01ec72 100644 --- a/public/java/src/org/broadinstitute/sting/utils/gvcf/GVCFHeaderBuilder.java +++ b/public/java/src/org/broadinstitute/sting/utils/gcf/GCFHeaderBuilder.java @@ -22,7 +22,7 @@ * OTHER DEALINGS IN THE SOFTWARE. */ -package org.broadinstitute.sting.utils.gvcf; +package org.broadinstitute.sting.utils.gcf; import org.broadinstitute.sting.utils.variantcontext.Allele; @@ -56,13 +56,13 @@ import java.util.Map; * @author Your Name * @since Date created */ -public class GVCFHeaderBuilder { +public class GCFHeaderBuilder { Map alleles = new HashMap(); Map strings = new HashMap(); Map samples = new HashMap(); - public GVCFHeader createHeader() { - return new GVCFHeader(alleles, strings, samples); + public GCFHeader createHeader() { + return new GCFHeader(alleles, strings, samples); } public int encodeString(final String chr) { return encode(strings, chr); } From fe5724b6ea7c77f3f38f35b2d29d118860b6fc2a Mon Sep 17 00:00:00 2001 From: Mark DePristo Date: Wed, 7 Sep 2011 23:27:08 -0400 Subject: [PATCH 09/41] Refactored indexing part of StandardVCFWriter into superclass -- Now other implementations of the VCFWriter can easily share common functions, such as writing an index on the fly --- .../utils/codecs/vcf/IndexingVCFWriter.java | 116 ++++++++++++++++++ .../utils/codecs/vcf/StandardVCFWriter.java | 107 +++++----------- 2 files changed, 148 insertions(+), 75 deletions(-) create mode 100644 public/java/src/org/broadinstitute/sting/utils/codecs/vcf/IndexingVCFWriter.java diff --git a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/IndexingVCFWriter.java b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/IndexingVCFWriter.java new file mode 100644 index 000000000..632bf8ed3 --- /dev/null +++ b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/IndexingVCFWriter.java @@ -0,0 +1,116 @@ +/* + * Copyright (c) 2011, The Broad Institute + * + * Permission is hereby granted, free of charge, to any person + * obtaining a copy of this software and associated documentation + * files (the "Software"), to deal in the Software without + * restriction, including without limitation the rights to use, + * copy, modify, merge, publish, distribute, sublicense, and/or sell + * copies of the Software, and to permit persons to whom the + * Software is furnished to do so, subject to the following + * conditions: + * + * The above copyright notice and this permission notice shall be + * included in all copies or substantial portions of the Software. + * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, + * EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES + * OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND + * NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT + * HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, + * WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING + * FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR + * OTHER DEALINGS IN THE SOFTWARE. + */ + +package org.broadinstitute.sting.utils.codecs.vcf; + +import org.broad.tribble.Tribble; +import org.broad.tribble.TribbleException; +import org.broad.tribble.index.DynamicIndexCreator; +import org.broad.tribble.index.Index; +import org.broad.tribble.index.IndexFactory; +import org.broad.tribble.util.LittleEndianOutputStream; +import org.broad.tribble.util.PositionalStream; +import org.broadinstitute.sting.utils.exceptions.ReviewedStingException; +import org.broadinstitute.sting.utils.variantcontext.VariantContext; + +import java.io.*; + +/** + * this class writes VCF files + */ +public abstract class IndexingVCFWriter implements VCFWriter { + final private File indexFile; + final private String name; + + private PositionalStream positionalStream; + private DynamicIndexCreator indexer; + private LittleEndianOutputStream idxStream; + + protected IndexingVCFWriter(String name, File location, OutputStream output, boolean enableOnTheFlyIndexing) { + this.name = name; + + if ( enableOnTheFlyIndexing ) { + indexFile = Tribble.indexFile(location); + try { + idxStream = new LittleEndianOutputStream(new FileOutputStream(indexFile)); + //System.out.println("Creating index on the fly for " + location); + indexer = new DynamicIndexCreator(IndexFactory.IndexBalanceApproach.FOR_SEEK_TIME); + indexer.initialize(location, indexer.defaultBinSize()); + positionalStream = new PositionalStream(output); + } catch ( IOException ex ) { + // No matter what we keep going, since we don't care if we can't create the index file + } + } else { + idxStream = null; + indexer = null; + positionalStream = null; + indexFile = null; + } + } + + public String getStreamName() { + return name; + } + + public abstract void writeHeader(VCFHeader header); + + /** + * attempt to close the VCF file + */ + public void close() { + // try to close the index stream (keep it separate to help debugging efforts) + if ( indexer != null ) { + try { + Index index = indexer.finalizeIndex(positionalStream.getPosition()); + index.write(idxStream); + idxStream.close(); + } catch (IOException e) { + throw new ReviewedStingException("Unable to close index for " + getStreamName(), e); + } + } + } + + /** + * add a record to the file + * + * @param vc the Variant Context object + */ + public void add(VariantContext vc) { + // if we are doing on the fly indexing, add the record ***before*** we write any bytes + if ( indexer != null ) + indexer.addFeature(vc, positionalStream.getPosition()); + } + + protected static final String writerName(File location, OutputStream stream) { + return location == null ? stream.toString() : location.getAbsolutePath(); + } + + protected static OutputStream openOutputStream(File location) { + try { + return new FileOutputStream(location); + } catch (FileNotFoundException e) { + throw new ReviewedStingException("Unable to create VCF file at location: " + location, e); + } + } +} diff --git a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/StandardVCFWriter.java b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/StandardVCFWriter.java index e28cd7598..ebcba9635 100755 --- a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/StandardVCFWriter.java +++ b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/StandardVCFWriter.java @@ -44,26 +44,19 @@ import java.util.*; /** * this class writes VCF files */ -public class StandardVCFWriter implements VCFWriter { +public class StandardVCFWriter extends IndexingVCFWriter { + // the print stream we're writing to + final protected BufferedWriter mWriter; + + // should we write genotypes or just sites? + final protected boolean doNotWriteGenotypes; // the VCF header we're storing protected VCFHeader mHeader = null; - // the print stream we're writing to - protected BufferedWriter mWriter; - protected PositionalStream positionalStream = null; - // were filters applied? protected boolean filtersWereAppliedToContext = false; - // should we write genotypes or just sites? - protected boolean doNotWriteGenotypes = false; - - protected DynamicIndexCreator indexer = null; - protected File indexFile = null; - LittleEndianOutputStream idxStream = null; - File location = null; - /** * create a VCF writer, given a file to write to * @@ -93,32 +86,22 @@ public class StandardVCFWriter implements VCFWriter { * @param doNotWriteGenotypes do not write genotypes */ public StandardVCFWriter(OutputStream output, boolean doNotWriteGenotypes) { - mWriter = new BufferedWriter(new OutputStreamWriter(output)); - this.doNotWriteGenotypes = doNotWriteGenotypes; + this(null, output, false, doNotWriteGenotypes); } public StandardVCFWriter(File location, OutputStream output, boolean enableOnTheFlyIndexing, boolean doNotWriteGenotypes) { - this.location = location; - - if ( enableOnTheFlyIndexing ) { - indexFile = Tribble.indexFile(location); - try { - idxStream = new LittleEndianOutputStream(new FileOutputStream(indexFile)); - //System.out.println("Creating index on the fly for " + location); - indexer = new DynamicIndexCreator(IndexFactory.IndexBalanceApproach.FOR_SEEK_TIME); - indexer.initialize(location, indexer.defaultBinSize()); - positionalStream = new PositionalStream(output); - output = positionalStream; - } catch ( IOException ex ) { - // No matter what we keep going, since we don't care if we can't create the index file - } - } - - //mWriter = new BufferedWriter(new OutputStreamWriter(new PositionalStream(output))); - mWriter = new BufferedWriter(new OutputStreamWriter(output)); + super(writerName(location, output), location, output, enableOnTheFlyIndexing); + mWriter = new BufferedWriter(new OutputStreamWriter(output)); // todo -- fix buffer size this.doNotWriteGenotypes = doNotWriteGenotypes; } + // -------------------------------------------------------------------------------- + // + // VCFWriter interface functions + // + // -------------------------------------------------------------------------------- + + @Override public void writeHeader(VCFHeader header) { mHeader = doNotWriteGenotypes ? new VCFHeader(header.getMetaData()) : header; @@ -158,44 +141,24 @@ public class StandardVCFWriter implements VCFWriter { mWriter.flush(); // necessary so that writing to an output stream will work } catch (IOException e) { - throw new TribbleException("IOException writing the VCF header to " + locationString(), e); + throw new ReviewedStingException("IOException writing the VCF header to " + getStreamName(), e); } } - private String locationString() { - return location == null ? mWriter.toString() : location.getAbsolutePath(); - } - /** * attempt to close the VCF file */ + @Override public void close() { // try to close the vcf stream try { mWriter.flush(); mWriter.close(); } catch (IOException e) { - throw new TribbleException("Unable to close " + locationString() + " because of " + e.getMessage()); + throw new ReviewedStingException("Unable to close " + getStreamName(), e); } - // try to close the index stream (keep it separate to help debugging efforts) - if ( indexer != null ) { - try { - Index index = indexer.finalizeIndex(positionalStream.getPosition()); - index.write(idxStream); - idxStream.close(); - } catch (IOException e) { - throw new TribbleException("Unable to close index for " + locationString() + " because of " + e.getMessage()); - } - } - } - - protected static OutputStream openOutputStream(File location) { - try { - return new FileOutputStream(location); - } catch (FileNotFoundException e) { - throw new TribbleException("Unable to create VCF file at location: " + location); - } + super.close(); } /** @@ -203,28 +166,17 @@ public class StandardVCFWriter implements VCFWriter { * * @param vc the Variant Context object */ + @Override public void add(VariantContext vc) { - add(vc, false); - } - - /** - * add a record to the file - * - * @param vc the Variant Context object - * @param refBaseShouldBeAppliedToEndOfAlleles *** THIS SHOULD BE FALSE EXCEPT FOR AN INDEL AT THE EXTREME BEGINNING OF A CONTIG (WHERE THERE IS NO PREVIOUS BASE, SO WE USE THE BASE AFTER THE EVENT INSTEAD) - */ - public void add(VariantContext vc, boolean refBaseShouldBeAppliedToEndOfAlleles) { if ( mHeader == null ) - throw new IllegalStateException("The VCF Header must be written before records can be added: " + locationString()); + throw new IllegalStateException("The VCF Header must be written before records can be added: " + getStreamName()); if ( doNotWriteGenotypes ) vc = VariantContext.modifyGenotypes(vc, null); try { - vc = VariantContext.createVariantContextWithPaddedAlleles(vc, refBaseShouldBeAppliedToEndOfAlleles); - - // if we are doing on the fly indexing, add the record ***before*** we write any bytes - if ( indexer != null ) indexer.addFeature(vc, positionalStream.getPosition()); + vc = VariantContext.createVariantContextWithPaddedAlleles(vc, false); + super.add(vc); Map alleleMap = new HashMap(vc.getAlleles().size()); alleleMap.put(Allele.NO_CALL, VCFConstants.EMPTY_ALLELE); // convenience for lookup @@ -317,10 +269,16 @@ public class StandardVCFWriter implements VCFWriter { mWriter.write("\n"); mWriter.flush(); // necessary so that writing to an output stream will work } catch (IOException e) { - throw new RuntimeException("Unable to write the VCF object to " + locationString()); + throw new RuntimeException("Unable to write the VCF object to " + getStreamName()); } } + // -------------------------------------------------------------------------------- + // + // implementation functions + // + // -------------------------------------------------------------------------------- + public static final String getFilterString(final VariantContext vc) { return getFilterString(vc, false); } @@ -531,12 +489,11 @@ public class StandardVCFWriter implements VCFWriter { } - public static int countOccurrences(char c, String s) { + private static int countOccurrences(char c, String s) { int count = 0; for (int i = 0; i < s.length(); i++) { count += s.charAt(i) == c ? 1 : 0; } return count; } - } From cd2c511c4ae8a7d13ca6fe3604308ca5fdea5c00 Mon Sep 17 00:00:00 2001 From: Mark DePristo Date: Wed, 7 Sep 2011 23:28:46 -0400 Subject: [PATCH 10/41] GCF improvements -- Support for streaming VCF writing via the VCFWriter interface -- GCF now has a header and a footer. The header is minimal, and contains a forward pointer to the position of the footer in the file. -- Readers now read the header, and then jump to the footer to get the rest of the "header" information -- Version now a field in GCF --- .../broadinstitute/sting/utils/gcf/GCF.java | 69 +++++----- .../sting/utils/gcf/GCFHeader.java | 49 +++++-- .../sting/utils/gcf/GCFWriter.java | 122 ++++++++++++++++++ 3 files changed, 198 insertions(+), 42 deletions(-) create mode 100644 public/java/src/org/broadinstitute/sting/utils/gcf/GCFWriter.java diff --git a/public/java/src/org/broadinstitute/sting/utils/gcf/GCF.java b/public/java/src/org/broadinstitute/sting/utils/gcf/GCF.java index 5ab241ebf..ef0d9ca42 100644 --- a/public/java/src/org/broadinstitute/sting/utils/gcf/GCF.java +++ b/public/java/src/org/broadinstitute/sting/utils/gcf/GCF.java @@ -79,8 +79,13 @@ public class GCF { } } - public GCF(DataInputStream inputStream, boolean skipGenotypes) throws IOException { + public GCF(DataInputStream inputStream, boolean skipGenotypes) throws IOException, EOFException { chromOffset = inputStream.readInt(); + + // have we reached the footer? + if ( chromOffset == GCFHeader.FOOTER_START_MARKER ) + throw new EOFException(); + start = inputStream.readInt(); stop = inputStream.readInt(); id = inputStream.readUTF(); @@ -106,6 +111,32 @@ public class GCF { throw new UserException.MalformedFile("Record not terminated by RECORD_TERMINATOR key"); } + public int write(DataOutputStream outputStream) throws IOException { + int startSize = outputStream.size(); + outputStream.writeInt(chromOffset); + outputStream.writeInt(start); + outputStream.writeInt(stop); + outputStream.writeUTF(id); + outputStream.writeByte(refPad); + writeIntArray(alleleOffsets, outputStream, true); + outputStream.writeFloat(qual); + outputStream.writeUTF(info); + outputStream.writeInt(filterOffset); + + int nGenotypes = genotypes.size(); + int expectedSizeOfGenotypes = nGenotypes == 0 ? 0 : genotypes.get(0).sizeInBytes() * nGenotypes; + outputStream.writeInt(nGenotypes); + outputStream.writeInt(expectedSizeOfGenotypes); + int obsSizeOfGenotypes = 0; + for ( GCFGenotype g : genotypes ) + obsSizeOfGenotypes += g.write(outputStream); + if ( obsSizeOfGenotypes != expectedSizeOfGenotypes ) + throw new RuntimeException("Expect and observed genotype sizes disagree! expect = " + expectedSizeOfGenotypes + " obs =" + obsSizeOfGenotypes); + + outputStream.writeInt(RECORD_TERMINATOR); + return outputStream.size() - startSize; + } + public VariantContext decode(final String source, final GCFHeader header) { final String contig = header.getString(chromOffset); alleleMap = header.getAlleles(alleleOffsets); @@ -154,31 +185,6 @@ public class GCF { public int getNAlleles() { return alleleOffsets.length; } - public int write(DataOutputStream outputStream) throws IOException { - int startSize = outputStream.size(); - outputStream.writeInt(chromOffset); - outputStream.writeInt(start); - outputStream.writeInt(stop); - outputStream.writeUTF(id); - outputStream.writeByte(refPad); - writeIntArray(alleleOffsets, outputStream, true); - outputStream.writeFloat(qual); - outputStream.writeUTF(info); - outputStream.writeInt(filterOffset); - - int nGenotypes = genotypes.size(); - int expectedSizeOfGenotypes = nGenotypes == 0 ? 0 : genotypes.get(0).sizeInBytes() * nGenotypes; - outputStream.writeInt(nGenotypes); - outputStream.writeInt(expectedSizeOfGenotypes); - int obsSizeOfGenotypes = 0; - for ( GCFGenotype g : genotypes ) - obsSizeOfGenotypes += g.write(outputStream); - if ( obsSizeOfGenotypes != expectedSizeOfGenotypes ) - throw new RuntimeException("Expect and observed genotype sizes disagree! expect = " + expectedSizeOfGenotypes + " obs =" + obsSizeOfGenotypes); - - outputStream.writeInt(RECORD_TERMINATOR); - return outputStream.size() - startSize; - } private final String infoFieldString(VariantContext vc, final GCFHeaderBuilder GCFHeaderBuilder) { StringBuilder s = new StringBuilder(); @@ -200,13 +206,14 @@ public class GCF { return s.toString(); } - private final static int BUFFER_SIZE = 1048576; // 2**20 - public static DataOutputStream createOutputStream(final File file) throws FileNotFoundException { - return new DataOutputStream(new BufferedOutputStream(new FileOutputStream(file), BUFFER_SIZE)); + protected final static int BUFFER_SIZE = 1048576; // 2**20 + + public static DataInputStream createDataInputStream(final InputStream stream) { + return new DataInputStream(new BufferedInputStream(stream, BUFFER_SIZE)); } - public static DataInputStream createInputStream(final File file) throws FileNotFoundException { - return new DataInputStream(new BufferedInputStream(new FileInputStream(file), BUFFER_SIZE)); + public static FileInputStream createFileInputStream(final File file) throws FileNotFoundException { + return new FileInputStream(file); } protected final static int[] readIntArray(final DataInputStream inputStream) throws IOException { diff --git a/public/java/src/org/broadinstitute/sting/utils/gcf/GCFHeader.java b/public/java/src/org/broadinstitute/sting/utils/gcf/GCFHeader.java index d0c765cc4..6d96eda56 100644 --- a/public/java/src/org/broadinstitute/sting/utils/gcf/GCFHeader.java +++ b/public/java/src/org/broadinstitute/sting/utils/gcf/GCFHeader.java @@ -30,9 +30,7 @@ import org.broadinstitute.sting.utils.codecs.vcf.VCFConstants; import org.broadinstitute.sting.utils.exceptions.UserException; import org.broadinstitute.sting.utils.variantcontext.Allele; -import java.io.DataInputStream; -import java.io.DataOutputStream; -import java.io.IOException; +import java.io.*; import java.util.*; /** @@ -65,25 +63,45 @@ import java.util.*; public class GCFHeader { final protected static Logger logger = Logger.getLogger(GCFHeader.class); - private static byte[] MAGIC_HEADER = "GVCF0.1\1".getBytes(); + public final static int GCF_VERSION = 1; + public final static byte[] GCF_FILE_START_MARKER = "GCF\1".getBytes(); + public final static int FOOTER_START_MARKER = -1; + public final static long HEADER_FORWARD_REFERENCE_OFFSET = GCF_FILE_START_MARKER.length + 4; // for the version + + final int version; + long footerPosition; final List alleles; final List strings; final List samples; final List> filters; public GCFHeader(final Map allelesIn, final Map stringIn, final Map samplesIn) { + version = GCF_VERSION; + footerPosition = 0; this.alleles = linearize(allelesIn); this.strings = linearize(stringIn); this.samples = linearize(samplesIn); this.filters = null; // not used with this constructor } - public GCFHeader(DataInputStream inputStream) throws IOException { - byte[] headerTest = new byte[MAGIC_HEADER.length]; + public GCFHeader(FileInputStream fileInputStream) throws IOException { + DataInputStream inputStream = new DataInputStream(fileInputStream); + byte[] headerTest = new byte[GCF_FILE_START_MARKER.length]; inputStream.read(headerTest); - if ( ! Arrays.equals(headerTest, MAGIC_HEADER) ) { - throw new UserException("Could not read GVCF file. MAGIC_HEADER missing. Saw " + headerTest); + if ( ! Arrays.equals(headerTest, GCF_FILE_START_MARKER) ) { + throw new UserException("Could not read GVCF file. GCF_FILE_START_MARKER missing. Saw " + new String(headerTest)); } else { + version = inputStream.readInt(); + logger.info("Read GCF version " + version); + footerPosition = inputStream.readLong(); + logger.info("Read footer position of " + footerPosition); + long lastPos = fileInputStream.getChannel().position(); + logger.info(" Last position is " + lastPos); + + // seek to the footer + fileInputStream.getChannel().position(footerPosition); + if ( inputStream.readInt() != FOOTER_START_MARKER ) + throw new UserException.MalformedFile("Malformed GCF file: couldn't find the footer marker"); alleles = stringsToAlleles(readStrings(inputStream)); strings = readStrings(inputStream); samples = readStrings(inputStream); @@ -91,19 +109,28 @@ public class GCFHeader { logger.info(String.format("String map of %d elements", strings.size())); logger.info(String.format("Sample map of %d elements", samples.size())); filters = initializeFilterCache(); + fileInputStream.getChannel().position(lastPos); } } - public int write(final DataOutputStream outputStream) throws IOException { + public static int writeHeader(final DataOutputStream outputStream) throws IOException { int startBytes = outputStream.size(); - outputStream.write(MAGIC_HEADER); + outputStream.write(GCF_FILE_START_MARKER); + outputStream.writeInt(GCF_VERSION); + outputStream.writeLong(0); + return outputStream.size() - startBytes; + } + + public int writeFooter(final DataOutputStream outputStream) throws IOException { + int startBytes = outputStream.size(); + outputStream.writeInt(FOOTER_START_MARKER); // has to be the same as chrom encoding write(outputStream, allelesToStrings(alleles)); write(outputStream, strings); write(outputStream, samples); return outputStream.size() - startBytes; } - public void write(DataOutputStream outputStream, List l) throws IOException { + private void write(DataOutputStream outputStream, List l) throws IOException { outputStream.writeInt(l.size()); for ( String elt : l ) outputStream.writeUTF(elt); } diff --git a/public/java/src/org/broadinstitute/sting/utils/gcf/GCFWriter.java b/public/java/src/org/broadinstitute/sting/utils/gcf/GCFWriter.java new file mode 100644 index 000000000..7ff6e27a2 --- /dev/null +++ b/public/java/src/org/broadinstitute/sting/utils/gcf/GCFWriter.java @@ -0,0 +1,122 @@ +/* + * Copyright (c) 2011, The Broad Institute + * + * Permission is hereby granted, free of charge, to any person + * obtaining a copy of this software and associated documentation + * files (the "Software"), to deal in the Software without + * restriction, including without limitation the rights to use, + * copy, modify, merge, publish, distribute, sublicense, and/or sell + * copies of the Software, and to permit persons to whom the + * Software is furnished to do so, subject to the following + * conditions: + * + * The above copyright notice and this permission notice shall be + * included in all copies or substantial portions of the Software. + * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, + * EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES + * OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND + * NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT + * HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, + * WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING + * FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR + * OTHER DEALINGS IN THE SOFTWARE. + */ + +package org.broadinstitute.sting.utils.gcf; + +import org.broadinstitute.sting.utils.codecs.vcf.IndexingVCFWriter; +import org.broadinstitute.sting.utils.codecs.vcf.VCFHeader; +import org.broadinstitute.sting.utils.exceptions.ReviewedStingException; +import org.broadinstitute.sting.utils.exceptions.UserException; +import org.broadinstitute.sting.utils.variantcontext.VariantContext; + +import java.io.*; + +/** + * GCFWriter implementing the VCFWriter interface + * @author Your Name + * @since Date created + */ +public class GCFWriter extends IndexingVCFWriter { + final boolean skipGenotypes; + final FileOutputStream fileOutputStream; + final DataOutputStream dataOutputStream; + final GCFHeaderBuilder gcfHeaderBuilder; + int nbytes = 0; + VCFHeader header = null; + File location; + + // -------------------------------------------------------------------------------- + // + // Constructors + // + // -------------------------------------------------------------------------------- + + public GCFWriter(File location, boolean enableOnTheFlyIndexing, boolean doNotWriteGenotypes) { + super(writerName(location, null), location, null, enableOnTheFlyIndexing); + this.location = location; + this.skipGenotypes = doNotWriteGenotypes; + + // write the output + try { + fileOutputStream = new FileOutputStream(location); + dataOutputStream = createDataOutputStream(fileOutputStream); + gcfHeaderBuilder = new GCFHeaderBuilder(); + } catch ( FileNotFoundException e ) { + throw new UserException.CouldNotCreateOutputFile(location, e); + } + } + + // -------------------------------------------------------------------------------- + // + // VCFWriter interface functions + // + // -------------------------------------------------------------------------------- + + @Override + public void writeHeader(VCFHeader header) { + this.header = header; + try { + nbytes += GCFHeader.writeHeader(dataOutputStream); + } catch ( IOException e ) { + throw new UserException.CouldNotCreateOutputFile(getStreamName(), "Couldn't write header", e); + } + } + + @Override + public void add(VariantContext vc) { + super.add(vc); + GCF gcf = new GCF(gcfHeaderBuilder, vc, skipGenotypes); + try { + nbytes += gcf.write(dataOutputStream); + } catch ( IOException e ) { + throw new UserException.CouldNotCreateOutputFile(getStreamName(), "Failed to add gcf record " + gcf + " to stream " + getStreamName(), e); + } + } + + @Override + public void close() { + // todo -- write out VCF header lines + GCFHeader gcfHeader = gcfHeaderBuilder.createHeader(); + try { + long headerPosition = nbytes; + nbytes += gcfHeader.writeFooter(dataOutputStream); + dataOutputStream.close(); + //System.out.println("Writing forward reference to " + headerPosition); + + RandomAccessFile raFile = new RandomAccessFile(location, "rw"); + raFile.seek(GCFHeader.HEADER_FORWARD_REFERENCE_OFFSET); + raFile.writeLong(headerPosition); + raFile.close(); + } catch ( IOException e ) { + throw new ReviewedStingException("Failed to close GCFWriter " + getStreamName(), e); + } + + super.close(); + } + + private static final DataOutputStream createDataOutputStream(final OutputStream stream) { + return new DataOutputStream(new BufferedOutputStream(stream, GCF.BUFFER_SIZE)); + } + +} From cb4a50b1478bb54c19ae57703733e4f330bd7a2f Mon Sep 17 00:00:00 2001 From: Ryan Poplin Date: Sat, 17 Sep 2011 16:42:49 -0400 Subject: [PATCH 12/41] Adding ability to try both small and large kmer lengths. Highest likelihood wins. --- .../sting/gatk/walkers/genotyper/UnifiedGenotyperEngine.java | 1 + 1 file changed, 1 insertion(+) diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/genotyper/UnifiedGenotyperEngine.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/genotyper/UnifiedGenotyperEngine.java index 87dd37bf6..a5cb00a5d 100755 --- a/public/java/src/org/broadinstitute/sting/gatk/walkers/genotyper/UnifiedGenotyperEngine.java +++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/genotyper/UnifiedGenotyperEngine.java @@ -40,6 +40,7 @@ import org.broadinstitute.sting.utils.codecs.vcf.VCFConstants; import org.broadinstitute.sting.utils.exceptions.ReviewedStingException; import org.broadinstitute.sting.utils.pileup.PileupElement; import org.broadinstitute.sting.utils.pileup.ReadBackedExtendedEventPileup; +import org.broadinstitute.sting.utils.pileup.ReadBackedExtendedEventPileupImpl; import org.broadinstitute.sting.utils.pileup.ReadBackedPileup; import org.broadinstitute.sting.utils.variantcontext.*; From 091c7197cdfa63e341e72230e41f187cf66931e0 Mon Sep 17 00:00:00 2001 From: Roger Zurawicki Date: Sun, 18 Sep 2011 19:21:51 -0400 Subject: [PATCH 13/41] Fixed memory leak and bug with deletions in clipping The ClippingOp clip cigar function would run into a endless loop if the parameter were out of the reads range, I stopped the bug. * There is no check to make sure the read coordinate are covered by the read though When Hard clipping to interval, I added a check for deletions. NOTE: method works for NA12878 WEx but needs to be more thoroughly tested/optimized --- .../sting/utils/clipreads/ClippingOp.java | 4 +++ .../sting/utils/clipreads/ReadClipper.java | 25 ++++++++++++++++--- 2 files changed, 26 insertions(+), 3 deletions(-) diff --git a/public/java/src/org/broadinstitute/sting/utils/clipreads/ClippingOp.java b/public/java/src/org/broadinstitute/sting/utils/clipreads/ClippingOp.java index bc200372f..951ab265c 100644 --- a/public/java/src/org/broadinstitute/sting/utils/clipreads/ClippingOp.java +++ b/public/java/src/org/broadinstitute/sting/utils/clipreads/ClippingOp.java @@ -324,6 +324,8 @@ public class ClippingOp { if (index <= stop && cigarElementIterator.hasNext()) cigarElement = cigarElementIterator.next(); + else + break; } // add the remaining cigar elements @@ -363,6 +365,8 @@ public class ClippingOp { index += shift; if (index < start && cigarElementIterator.hasNext()) cigarElement = cigarElementIterator.next(); + else + break; } // check if we are hard clipping indels diff --git a/public/java/src/org/broadinstitute/sting/utils/clipreads/ReadClipper.java b/public/java/src/org/broadinstitute/sting/utils/clipreads/ReadClipper.java index 26c25850a..a8a3fad9e 100644 --- a/public/java/src/org/broadinstitute/sting/utils/clipreads/ReadClipper.java +++ b/public/java/src/org/broadinstitute/sting/utils/clipreads/ReadClipper.java @@ -1,6 +1,8 @@ package org.broadinstitute.sting.utils.clipreads; import com.google.java.contract.Requires; +import net.sf.samtools.CigarElement; +import net.sf.samtools.CigarOperator; import net.sf.samtools.SAMRecord; import org.broadinstitute.sting.utils.exceptions.ReviewedStingException; import org.broadinstitute.sting.utils.sam.ReadUtils; @@ -56,17 +58,34 @@ public class ReadClipper { return hardClipByReferenceCoordinates(refStart, -1); } + private int numDeletions(SAMRecord read) { + int result = 0; + for (CigarElement e: read.getCigar().getCigarElements()) { + if ( e.getOperator() == CigarOperator.DELETION || e.getOperator() == CigarOperator.D ) + result =+ e.getLength(); + } + return result; + } + private SAMRecord hardClipByReferenceCoordinates(int refStart, int refStop) { int start = (refStart < 0) ? 0 : ReadUtils.getReadCoordinateForReferenceCoordinate(read, refStart); - int stop = (refStop < 0) ? read.getReadLength() - 1 : ReadUtils.getReadCoordinateForReferenceCoordinate(read, refStop); + int stop = (refStop < 0) ? read.getReadLength() - 1: ReadUtils.getReadCoordinateForReferenceCoordinate(read, refStop); - if (start < 0 || stop > read.getReadLength() - 1) + if (start < 0 || stop > read.getReadLength() - 1 + numDeletions(read)) throw new ReviewedStingException("Trying to clip before the start or after the end of a read"); - // TODO add requires statement/check in the Hardclip function + // TODO add check in the Hardclip function if ( start > stop ) stop = ReadUtils.getReadCoordinateForReferenceCoordinate(read, ReadUtils.getRefCoordSoftUnclippedEnd(read)); + + //This tries to fix the bug where the deletion is counted a read base and as a result, the hardCLipper runs into + //an endless loop when hard clipping the cigar string because the read coordinates are not covered by the read + stop -= numDeletions(read); + if ( start > stop ) + start -= numDeletions(read); + + //System.out.println("Clipping start/stop: " + start + "/" + stop); this.addOp(new ClippingOp(start, stop)); SAMRecord clippedRead = clipRead(ClippingRepresentation.HARDCLIP_BASES); From 85626e7a5dbae8a263b8e2ff2e64bd25656d6e9c Mon Sep 17 00:00:00 2001 From: Eric Banks Date: Mon, 19 Sep 2011 12:24:05 -0400 Subject: [PATCH 14/41] We no longer want people to use the August 2010 Dindel calls for indel realignment but instead Guillermo's new whole genome bi-allelic indel calls; updating the bundle accordingly. Also, there was some confusion by the 1000G data processing folks as to exactly what these indel files are, so I've renamed them so that it's clear. Wiki updated too. --- .../sting/queue/qscripts/GATKResourcesBundle.scala | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/public/scala/qscript/org/broadinstitute/sting/queue/qscripts/GATKResourcesBundle.scala b/public/scala/qscript/org/broadinstitute/sting/queue/qscripts/GATKResourcesBundle.scala index 59c00b8cd..e8b8258c1 100755 --- a/public/scala/qscript/org/broadinstitute/sting/queue/qscripts/GATKResourcesBundle.scala +++ b/public/scala/qscript/org/broadinstitute/sting/queue/qscripts/GATKResourcesBundle.scala @@ -131,11 +131,11 @@ class GATKResourcesBundle extends QScript { addResource(new Resource("/humgen/gsa-hpprojects/GATK/data/Comparisons/Validated/HapMap/3.3/genotypes_r27_nr.b37_fwd.vcf", "hapmap_3.3", b37, true, true)) - addResource(new Resource("/humgen/gsa-hpprojects/GATK/data/Comparisons/Unvalidated/AFR+EUR+ASN+1KG.dindel_august_release_merged_pilot1.20110126.sites.vcf", - "1000G_indels_for_realignment", b37, true, false)) + addResource(new Resource("/humgen/1kg/processing/official_release/phase1/ALL.wgs.VQSR_consensus_biallelic.20101123.indels.sites.vcf", + "1000G_biallelic.indels", b37, true, false)) addResource(new Resource("/humgen/gsa-hpprojects/GATK/data/Comparisons/Validated/Mills_Devine_Indels_2011/ALL.wgs.indels_mills_devine_hg19_leftAligned_collapsed_double_hit.sites.vcf", - "indels_mills_devine", b37, true, true)) + "Mills_Devine_2hit.indels", b37, true, true)) // // example call set for wiki tutorial From 8143def292a49844ab3ff302fbb00f5c866299f7 Mon Sep 17 00:00:00 2001 From: Christopher Hartl Date: Mon, 19 Sep 2011 10:29:06 -0400 Subject: [PATCH 15/41] Fix the -T argument in the DepthOfCoverage docs Add documentation for the RefSeqCodec, pointing users to the wiki page describing how to create the file --- .../coverage/DepthOfCoverageWalker.java | 9 ++++--- .../utils/codecs/refseq/RefSeqCodec.java | 24 +++++++++++++++---- 2 files changed, 26 insertions(+), 7 deletions(-) diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/coverage/DepthOfCoverageWalker.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/coverage/DepthOfCoverageWalker.java index 86f97a36c..664c319ab 100644 --- a/public/java/src/org/broadinstitute/sting/gatk/walkers/coverage/DepthOfCoverageWalker.java +++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/coverage/DepthOfCoverageWalker.java @@ -63,9 +63,12 @@ import java.util.*; *

Input

*

* One or more bam files (with proper headers) to be analyzed for coverage statistics - * (Optional) A REFSEQ Rod to aggregate coverage to the gene level *

- * + *

+ *(Optional) A REFSEQ Rod to aggregate coverage to the gene level + *

+ * (for information about creating the REFSEQ Rod, please consult the RefSeqCodec documentation) + *

*

Output

*

* Tables pertaining to different coverage summaries. Suffix on the table files declares the contents: @@ -93,7 +96,7 @@ import java.util.*; * 

  * java -Xmx2g -jar GenomeAnalysisTK.jar \
  *   -R ref.fasta \
- *   -T VariantEval \
+ *   -T DepthOfCoverage \
  *   -o file_name_base \
  *   -I input_bams.list
  *   [-geneList refSeq.sorted.txt] \
diff --git a/public/java/src/org/broadinstitute/sting/utils/codecs/refseq/RefSeqCodec.java b/public/java/src/org/broadinstitute/sting/utils/codecs/refseq/RefSeqCodec.java
index d94d9ff84..f142fa5aa 100644
--- a/public/java/src/org/broadinstitute/sting/utils/codecs/refseq/RefSeqCodec.java
+++ b/public/java/src/org/broadinstitute/sting/utils/codecs/refseq/RefSeqCodec.java
@@ -12,19 +12,35 @@ import org.broadinstitute.sting.utils.exceptions.UserException;
 import java.util.ArrayList;
 
 /**
- * TODO FOR CHRIS HARTL
+ * Allows for reading in RefSeq information
  *
  * 

- * Codec Description
+ * Parses a sorted UCSC RefSeq file (see below) into relevant features: the gene name, the unique gene name (if multiple transcrips get separate entries), exons, gene start/stop, coding start/stop,
+ * strandedness of transcription. 
  * 

  *
  * 

- * See also: link to file specification
+ * Instructions for generating a RefSeq file for use with the RefSeq codec can be found on the Wiki here
+ * http://www.broadinstitute.org/gsa/wiki/index.php/RefSeq
  * 

+ * 
 Usage 

+ * The RefSeq Rod can be bound as any other rod, and is specified by REFSEQ, for example
+ * 
+ * -refSeqBinding:REFSEQ /path/to/refSeq.txt
+ * 

+ *
+ * You will need to consult individual walkers for the binding name ("refSeqBinding", above)
  *
  * 
File format example

+ * If you want to define your own file for use, the format is (tab delimited):
+ * bin, name, chrom, strand, transcription start, transcription end, coding start, coding end, num exons, exon starts, exon ends, id, alt. name, coding start status (complete/incomplete), coding end status (complete,incomplete)
+ * and exon frames, for example:
+ * 
+ * 76 NM_001011874 1 - 3204562 3661579 3206102 3661429 3 3204562,3411782,3660632, 3207049,3411982,3661579, 0 Xkr4 cmpl cmpl 1,2,0,
+ * 

+ * for more information see here
  * 

- *     A BAM file containing exactly one sample.
+ *     
  * 

  *
  * @author Mark DePristo

From 5e832254a4e024378f7fdee252abf7df9e289c6a Mon Sep 17 00:00:00 2001
From: Mauricio Carneiro 
Date: Mon, 19 Sep 2011 13:28:41 -0400
Subject: [PATCH 16/41] Fixing ReadAndInterval overlap comments.

---
 .../sting/utils/sam/ReadUtils.java            | 19 ++++++++++++++-----
 1 file changed, 14 insertions(+), 5 deletions(-)

diff --git a/public/java/src/org/broadinstitute/sting/utils/sam/ReadUtils.java b/public/java/src/org/broadinstitute/sting/utils/sam/ReadUtils.java
index 62bbb0307..18fcdabf2 100644
--- a/public/java/src/org/broadinstitute/sting/utils/sam/ReadUtils.java
+++ b/public/java/src/org/broadinstitute/sting/utils/sam/ReadUtils.java
@@ -118,31 +118,40 @@ public class ReadUtils {
     /**
      * This enum represents all the different ways in which a read can overlap an interval.
      *
-     * NO_OVERLAP:
+     * NO_OVERLAP_CONTIG:
+     * read and interval are in different contigs.
+     *
+     * NO_OVERLAP_LEFT:
+     * the read does not overlap the interval.
+     *
+     *                        |----------------| (interval)
+     *   <---------------->                      (read)
+     *
+     * NO_OVERLAP_RIGHT:
      * the read does not overlap the interval.
      *
      *   |----------------|                      (interval)
      *                        <----------------> (read)
      *
-     * LEFT_OVERLAP:
+     * OVERLAP_LEFT:
      * the read starts before the beginning of the interval but ends inside of it
      *
      *          |----------------| (interval)
      *   <---------------->        (read)
      *
-     * RIGHT_OVERLAP:
+     * OVERLAP_RIGHT:
      * the read starts inside the interval but ends outside of it
      *
      *   |----------------|     (interval)
      *       <----------------> (read)
      *
-     * FULL_OVERLAP:
+     * OVERLAP_LEFT_AND_RIGHT:
      * the read starts before the interval and ends after the interval
      *
      *      |-----------|     (interval)
      *  <-------------------> (read)
      *
-     * CONTAINED:
+     * OVERLAP_CONTAINED:
      * the read starts and ends inside the interval
      *
      *  |----------------|     (interval)

From ba150570f3d7747256f634a2828ab673a98953f7 Mon Sep 17 00:00:00 2001
From: Eric Banks 
Date: Mon, 19 Sep 2011 13:30:32 -0400
Subject: [PATCH 17/41] Updating to use new rod system syntax plus name change
 for CountRODs

---
 .../sting/queue/qscripts/GATKResourcesBundle.scala          | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/public/scala/qscript/org/broadinstitute/sting/queue/qscripts/GATKResourcesBundle.scala b/public/scala/qscript/org/broadinstitute/sting/queue/qscripts/GATKResourcesBundle.scala
index e8b8258c1..036a77b58 100755
--- a/public/scala/qscript/org/broadinstitute/sting/queue/qscripts/GATKResourcesBundle.scala
+++ b/public/scala/qscript/org/broadinstitute/sting/queue/qscripts/GATKResourcesBundle.scala
@@ -300,9 +300,9 @@ class GATKResourcesBundle extends QScript {
     bamFile = bamIn
   }
 
-  class IndexVCF(@Input vcf: File, @Input ref: File) extends CountRod with UNIVERSAL_GATK_ARGS {
+  class IndexVCF(@Input vcf: File, @Input ref: File) extends CountRODs with UNIVERSAL_GATK_ARGS {
     //@Output val vcfIndex: File = swapExt(vcf.getParent, vcf, ".vcf", ".vcf.idx")
-    this.rodBind :+= RodBind(vcf.getName, "VCF", vcf)
+    this.rod :+= vcf
     this.reference_sequence = ref
   }
 
@@ -313,7 +313,7 @@ class GATKResourcesBundle extends QScript {
   }
 
   class MakeDBSNP129(@Input dbsnp: File, @Input ref: File, @Output dbsnp129: File) extends SelectVariants with UNIVERSAL_GATK_ARGS {
-    this.rodBind :+= RodBind("variant", "VCF", dbsnp)
+    this.variant = dbsnp
     this.select ++= List("\"dbSNPBuildID <= 129\"")
     this.reference_sequence = ref
     this.out = dbsnp129

From 080c9575470c505e10f7b09d59fa22fcb668867d Mon Sep 17 00:00:00 2001
From: Mauricio Carneiro 
Date: Mon, 19 Sep 2011 13:53:08 -0400
Subject: [PATCH 18/41] Fixing contracts for SoftUnclippedEnd utils

Now accepts reads that are entirely contained inside an insertion.
---
 .../broadinstitute/sting/utils/sam/ReadUtils.java    | 12 ++++++++++--
 1 file changed, 10 insertions(+), 2 deletions(-)

diff --git a/public/java/src/org/broadinstitute/sting/utils/sam/ReadUtils.java b/public/java/src/org/broadinstitute/sting/utils/sam/ReadUtils.java
index 18fcdabf2..2de17db14 100644
--- a/public/java/src/org/broadinstitute/sting/utils/sam/ReadUtils.java
+++ b/public/java/src/org/broadinstitute/sting/utils/sam/ReadUtils.java
@@ -667,7 +667,7 @@ public class ReadUtils {
             return ReadAndIntervalOverlap.OVERLAP_RIGHT;
     }
 
-    @Ensures({"result >= read.getUnclippedStart()", "result <= read.getUnclippedEnd()"})
+    @Ensures({"(result >= read.getUnclippedStart() && result <= read.getUnclippedEnd()) || readIsEntirelyInsertion(read)"})
     public static int getRefCoordSoftUnclippedStart(SAMRecord read) {
         int start = read.getUnclippedStart();
         for (CigarElement cigarElement : read.getCigar().getCigarElements()) {
@@ -679,7 +679,7 @@ public class ReadUtils {
         return start;
     }
 
-    @Ensures({"result >= read.getUnclippedStart()", "result <= read.getUnclippedEnd()"})
+    @Ensures({"(result >= read.getUnclippedStart() && result <= read.getUnclippedEnd()) || readIsEntirelyInsertion(read)"})
     public static int getRefCoordSoftUnclippedEnd(SAMRecord read) {
         int stop = read.getUnclippedStart();
         int shift = 0;
@@ -695,6 +695,14 @@ public class ReadUtils {
         return (lastOperator == CigarOperator.HARD_CLIP) ? stop-1 : stop+shift-1 ;
     }
 
+    private static boolean readIsEntirelyInsertion(SAMRecord read) {
+        for (CigarElement cigarElement : read.getCigar().getCigarElements()) {
+            if (cigarElement.getOperator() != CigarOperator.INSERTION)
+                return false;
+        }
+        return true;
+    }
+
     /**
      * Looks for a read coordinate that corresponds to the reference coordinate in the soft clipped region before
      * the alignment start of the read.

From 56106d54ed620965aea0b39052de43c81671c817 Mon Sep 17 00:00:00 2001
From: Mauricio Carneiro 
Date: Mon, 19 Sep 2011 14:00:00 -0400
Subject: [PATCH 19/41] Changing ReadUtils behavior to comply with
 GenomeLocParser

Now the functions getRefCoordSoftUnclippedStart and getRefCoordSoftUnclippedEnd will return getUnclippedStart if the read is all contained within an insertion. Updated the contracts accordingly. This should give the same behavior as the GenomeLocParser now.
---
 .../src/org/broadinstitute/sting/utils/sam/ReadUtils.java | 8 ++++++--
 1 file changed, 6 insertions(+), 2 deletions(-)

diff --git a/public/java/src/org/broadinstitute/sting/utils/sam/ReadUtils.java b/public/java/src/org/broadinstitute/sting/utils/sam/ReadUtils.java
index 2de17db14..5d3ef3086 100644
--- a/public/java/src/org/broadinstitute/sting/utils/sam/ReadUtils.java
+++ b/public/java/src/org/broadinstitute/sting/utils/sam/ReadUtils.java
@@ -667,7 +667,7 @@ public class ReadUtils {
             return ReadAndIntervalOverlap.OVERLAP_RIGHT;
     }
 
-    @Ensures({"(result >= read.getUnclippedStart() && result <= read.getUnclippedEnd()) || readIsEntirelyInsertion(read)"})
+    @Ensures({"result >= read.getUnclippedStart()", "result <= read.getUnclippedEnd() || readIsEntirelyInsertion(read)"})
     public static int getRefCoordSoftUnclippedStart(SAMRecord read) {
         int start = read.getUnclippedStart();
         for (CigarElement cigarElement : read.getCigar().getCigarElements()) {
@@ -679,9 +679,13 @@ public class ReadUtils {
         return start;
     }
 
-    @Ensures({"(result >= read.getUnclippedStart() && result <= read.getUnclippedEnd()) || readIsEntirelyInsertion(read)"})
+    @Ensures({"result >= read.getUnclippedStart()", "result <= read.getUnclippedEnd() || readIsEntirelyInsertion(read)"})
     public static int getRefCoordSoftUnclippedEnd(SAMRecord read) {
         int stop = read.getUnclippedStart();
+
+        if (readIsEntirelyInsertion(read))
+            return stop;
+
         int shift = 0;
         CigarOperator lastOperator = null;
         for (CigarElement cigarElement : read.getCigar().getCigarElements()) {

From 61b89e236ab13b073a3572e983b6c730efd5331e Mon Sep 17 00:00:00 2001
From: Khalid Shakir 
Date: Tue, 20 Sep 2011 00:14:35 -0400
Subject: [PATCH 20/41] To work around potential problem with invalid
 javax.mail 1.4.1 in ivy cache, added explicit javax.mail 1.4.4 along with
 build.xml code to remove 1.4.1.

---
 build.xml | 8 ++++++++
 ivy.xml   | 6 ++----
 2 files changed, 10 insertions(+), 4 deletions(-)

diff --git a/build.xml b/build.xml
index e5ad9daf0..1f26e7b7a 100644
--- a/build.xml
+++ b/build.xml
@@ -163,6 +163,14 @@
       
       
       
+      
+      
+      
+      
     
 
     
diff --git a/ivy.xml b/ivy.xml
index 115f4062a..f90b9a010 100644
--- a/ivy.xml
+++ b/ivy.xml
@@ -15,10 +15,8 @@
     
     
 
-    
-      
-      
-    
+    
+    
     
     
     

From 5d0705acd654cfed6e9bf2ba690a0c75ee5a50d8 Mon Sep 17 00:00:00 2001
From: Ryan Poplin 
Date: Tue, 20 Sep 2011 09:07:28 -0400
Subject: [PATCH 21/41] Adding quality scores to the VCF records created by the
 Haplotype Caller

---
 .../sting/gatk/walkers/genotyper/UnifiedGenotyperEngine.java     | 1 -
 1 file changed, 1 deletion(-)

diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/genotyper/UnifiedGenotyperEngine.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/genotyper/UnifiedGenotyperEngine.java
index a5cb00a5d..87dd37bf6 100755
--- a/public/java/src/org/broadinstitute/sting/gatk/walkers/genotyper/UnifiedGenotyperEngine.java
+++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/genotyper/UnifiedGenotyperEngine.java
@@ -40,7 +40,6 @@ import org.broadinstitute.sting.utils.codecs.vcf.VCFConstants;
 import org.broadinstitute.sting.utils.exceptions.ReviewedStingException;
 import org.broadinstitute.sting.utils.pileup.PileupElement;
 import org.broadinstitute.sting.utils.pileup.ReadBackedExtendedEventPileup;
-import org.broadinstitute.sting.utils.pileup.ReadBackedExtendedEventPileupImpl;
 import org.broadinstitute.sting.utils.pileup.ReadBackedPileup;
 import org.broadinstitute.sting.utils.variantcontext.*;
 

From b7511c5ff3b36e16037bfbbbd17b9fd4c9ea47af Mon Sep 17 00:00:00 2001
From: Mark DePristo 
Date: Tue, 20 Sep 2011 10:53:18 -0400
Subject: [PATCH 22/41] Fixed long-standing bug in tribble index creation

-- Previously, on the fly indices didn't have dictionary set on the fly, so the GATK would read, add dictionary, and rewrite the index.  This is now fixed, so that the on the fly index contains the reference dictionary when first written, avoiding the unnecessary read and write
-- Added a GenomeAnalysisEngine and Walker function called getMasterSequenceDictionary() that fetches the reference sequence dictionary.  This can be used conveniently everywhere, and is what's written into the Tribble index
-- Refactored tribble index utilities from RMDTrackBuilder into IndexDictionaryUtils
-- VCFWriter now requires the master sequence dictionary
-- Updated walkers that create VCFWriters to provide the master sequence dictionary
---
 .../sting/gatk/GenomeAnalysisEngine.java      |   8 ++
 .../gatk/io/storage/VCFWriterStorage.java     |   4 +-
 .../sting/gatk/io/stubs/VCFWriterStub.java    |  10 ++
 .../gatk/refdata/indexer/RMDIndexer.java      |   2 +-
 .../refdata/tracks/IndexDictionaryUtils.java  | 106 ++++++++++++++++
 .../gatk/refdata/tracks/RMDTrackBuilder.java  | 113 ++++--------------
 .../sting/gatk/walkers/Walker.java            |  10 ++
 .../variantutils/LiftoverVariants.java        |   2 +-
 .../variantutils/RandomlySplitVariants.java   |   2 +-
 .../utils/codecs/vcf/IndexingVCFWriter.java   |  38 ++++--
 .../utils/codecs/vcf/StandardVCFWriter.java   |  26 ++--
 .../sting/utils/gcf/GCFWriter.java            |   5 +-
 .../org/broadinstitute/sting/WalkerTest.java  |   2 +-
 .../tracks/RMDTrackBuilderUnitTest.java       |   4 +-
 .../codecs/vcf/IndexFactoryUnitTest.java      |  22 +++-
 .../utils/genotype/vcf/VCFWriterUnitTest.java |   5 +-
 16 files changed, 228 insertions(+), 131 deletions(-)
 create mode 100644 public/java/src/org/broadinstitute/sting/gatk/refdata/tracks/IndexDictionaryUtils.java

diff --git a/public/java/src/org/broadinstitute/sting/gatk/GenomeAnalysisEngine.java b/public/java/src/org/broadinstitute/sting/gatk/GenomeAnalysisEngine.java
index 5b9ebd99b..972943e26 100755
--- a/public/java/src/org/broadinstitute/sting/gatk/GenomeAnalysisEngine.java
+++ b/public/java/src/org/broadinstitute/sting/gatk/GenomeAnalysisEngine.java
@@ -929,6 +929,14 @@ public class GenomeAnalysisEngine {
         return readsDataSource.getHeader(reader);
     }
 
+    /**
+     * Gets the master sequence dictionary for this GATK engine instance
+     * @return a never-null dictionary listing all of the contigs known to this engine instance
+     */
+    public SAMSequenceDictionary getMasterSequenceDictionary() {
+        return getReferenceDataSource().getReference().getSequenceDictionary();
+    }
+
     /**
      * Returns data source object encapsulating all essential info and handlers used to traverse
      * reads; header merger, individual file readers etc can be accessed through the returned data source object.
diff --git a/public/java/src/org/broadinstitute/sting/gatk/io/storage/VCFWriterStorage.java b/public/java/src/org/broadinstitute/sting/gatk/io/storage/VCFWriterStorage.java
index ebb4cbe66..4ca7b935f 100644
--- a/public/java/src/org/broadinstitute/sting/gatk/io/storage/VCFWriterStorage.java
+++ b/public/java/src/org/broadinstitute/sting/gatk/io/storage/VCFWriterStorage.java
@@ -46,7 +46,7 @@ public class VCFWriterStorage implements Storage, VCFWriter {
         else if ( stub.getOutputStream() != null ) {
             this.file = null;
             this.stream = stub.getOutputStream();
-            writer = new StandardVCFWriter(stream, stub.doNotWriteGenotypes());
+            writer = new StandardVCFWriter(stream, stub.getMasterSequenceDictionary(), stub.doNotWriteGenotypes());
         }
         else
             throw new ReviewedStingException("Unable to create target to which to write; storage was provided with neither a file nor a stream.");
@@ -71,7 +71,7 @@ public class VCFWriterStorage implements Storage, VCFWriter {
         }
 
         // The GATK/Tribble can't currently index block-compressed files on the fly.  Disable OTF indexing even if the user explicitly asked for it.
-        return new StandardVCFWriter(file, this.stream, indexOnTheFly && !stub.isCompressed(), stub.doNotWriteGenotypes());
+        return new StandardVCFWriter(file, this.stream, stub.getMasterSequenceDictionary(), indexOnTheFly && !stub.isCompressed(), stub.doNotWriteGenotypes());
     }
 
 
diff --git a/public/java/src/org/broadinstitute/sting/gatk/io/stubs/VCFWriterStub.java b/public/java/src/org/broadinstitute/sting/gatk/io/stubs/VCFWriterStub.java
index 936243f9d..82cb43634 100755
--- a/public/java/src/org/broadinstitute/sting/gatk/io/stubs/VCFWriterStub.java
+++ b/public/java/src/org/broadinstitute/sting/gatk/io/stubs/VCFWriterStub.java
@@ -25,6 +25,7 @@
 
 package org.broadinstitute.sting.gatk.io.stubs;
 
+import net.sf.samtools.SAMSequenceDictionary;
 import net.sf.samtools.SAMSequenceRecord;
 import org.broadinstitute.sting.gatk.CommandLineExecutable;
 import org.broadinstitute.sting.gatk.GenomeAnalysisEngine;
@@ -150,6 +151,15 @@ public class VCFWriterStub implements Stub, VCFWriter {
         return isCompressed;
     }
 
+    /**
+     * Gets the master sequence dictionary from the engine associated with this stub
+     * @link GenomeAnalysisEngine.getMasterSequenceDictionary
+     * @return
+     */
+    public SAMSequenceDictionary getMasterSequenceDictionary() {
+        return engine.getMasterSequenceDictionary();
+    }
+
     /**
      * Should we tell the VCF writer not to write genotypes?
      * @return true if the writer should not write genotypes.
diff --git a/public/java/src/org/broadinstitute/sting/gatk/refdata/indexer/RMDIndexer.java b/public/java/src/org/broadinstitute/sting/gatk/refdata/indexer/RMDIndexer.java
index 029800aea..9e5a95d10 100644
--- a/public/java/src/org/broadinstitute/sting/gatk/refdata/indexer/RMDIndexer.java
+++ b/public/java/src/org/broadinstitute/sting/gatk/refdata/indexer/RMDIndexer.java
@@ -101,7 +101,7 @@ public class RMDIndexer extends CommandLineProgram {
         Index index = IndexFactory.createIndex(inputFileSource, codec, approach);
 
         // add writing of the sequence dictionary, if supplied
-        builder.setIndexSequenceDictionary(inputFileSource, index, ref.getSequenceDictionary(), indexFile, false);
+        builder.validateAndUpdateIndexSequenceDictionary(inputFileSource, index, ref.getSequenceDictionary());
 
         // create the output stream, and write the index
         LittleEndianOutputStream stream = new LittleEndianOutputStream(new FileOutputStream(indexFile));
diff --git a/public/java/src/org/broadinstitute/sting/gatk/refdata/tracks/IndexDictionaryUtils.java b/public/java/src/org/broadinstitute/sting/gatk/refdata/tracks/IndexDictionaryUtils.java
new file mode 100644
index 000000000..d133439dc
--- /dev/null
+++ b/public/java/src/org/broadinstitute/sting/gatk/refdata/tracks/IndexDictionaryUtils.java
@@ -0,0 +1,106 @@
+/*
+ * Copyright (c) 2011, The Broad Institute
+ *
+ * Permission is hereby granted, free of charge, to any person
+ * obtaining a copy of this software and associated documentation
+ * files (the "Software"), to deal in the Software without
+ * restriction, including without limitation the rights to use,
+ * copy, modify, merge, publish, distribute, sublicense, and/or sell
+ * copies of the Software, and to permit persons to whom the
+ * Software is furnished to do so, subject to the following
+ * conditions:
+ *
+ * The above copyright notice and this permission notice shall be
+ * included in all copies or substantial portions of the Software.
+ * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND,
+ * EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES
+ * OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND
+ * NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT
+ * HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY,
+ * WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING
+ * FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR
+ * OTHER DEALINGS IN THE SOFTWARE.
+ */
+
+package org.broadinstitute.sting.gatk.refdata.tracks;
+
+import net.sf.samtools.SAMSequenceDictionary;
+import net.sf.samtools.SAMSequenceRecord;
+import org.apache.log4j.Logger;
+import org.broad.tribble.index.Index;
+import org.broadinstitute.sting.gatk.arguments.ValidationExclusion;
+import org.broadinstitute.sting.utils.SequenceDictionaryUtils;
+
+import java.util.LinkedHashSet;
+import java.util.Map;
+import java.util.Set;
+import java.util.TreeSet;
+
+/**
+ * Utilities for working with Sequence Dictionaries embedded in tribble indices
+ *
+ * @author Your Name
+ * @since Date created
+ */
+public class IndexDictionaryUtils {
+    private final static Logger logger = Logger.getLogger(IndexDictionaryUtils.class);
+
+    // a constant we use for marking sequence dictionary entries in the Tribble index property list
+    public static final String SequenceDictionaryPropertyPredicate = "DICT:";
+
+    /**
+     * get the sequence dictionary from the track, if available.  If not, make it from the contig list that is always in the index
+     * @param index the index file to use
+     * @return a SAMSequenceDictionary if available, null if unavailable
+     */
+    public static SAMSequenceDictionary getSequenceDictionaryFromProperties(Index index) {
+        SAMSequenceDictionary dict = new SAMSequenceDictionary();
+        for (Map.Entry entry : index.getProperties().entrySet()) {
+            if (entry.getKey().startsWith(SequenceDictionaryPropertyPredicate))
+                dict.addSequence(new SAMSequenceRecord(entry.getKey().substring(SequenceDictionaryPropertyPredicate.length() , entry.getKey().length()),
+                        Integer.valueOf(entry.getValue())));
+        }
+        return dict;
+    }
+
+    /**
+     * create the sequence dictionary with the contig list; a backup approach
+     * @param index the index file to use
+     * @param dict the sequence dictionary to add contigs to
+     * @return the filled-in sequence dictionary
+     */
+    static SAMSequenceDictionary createSequenceDictionaryFromContigList(Index index, SAMSequenceDictionary dict) {
+        LinkedHashSet seqNames = index.getSequenceNames();
+        if (seqNames == null) {
+            return dict;
+        }
+        for (String name : seqNames) {
+            SAMSequenceRecord seq = new SAMSequenceRecord(name, 0);
+            dict.addSequence(seq);
+        }
+        return dict;
+    }
+
+    public static void setIndexSequenceDictionary(Index index, SAMSequenceDictionary dict) {
+        for ( SAMSequenceRecord seq : dict.getSequences() ) {
+            final String contig = IndexDictionaryUtils.SequenceDictionaryPropertyPredicate + seq.getSequenceName();
+            final String length = String.valueOf(seq.getSequenceLength());
+            index.addProperty(contig,length);
+        }
+    }
+
+    public static void validateTrackSequenceDictionary(final String trackName,
+                                                       final SAMSequenceDictionary trackDict,
+                                                       final SAMSequenceDictionary referenceDict,
+                                                       final ValidationExclusion.TYPE validationExclusionType ) {
+        // if the sequence dictionary is empty (as well as null which means it doesn't have a dictionary), skip validation
+        if (trackDict == null || trackDict.size() == 0)
+            logger.info("Track " + trackName + " doesn't have a sequence dictionary built in, skipping dictionary validation");
+        else {
+            Set trackSequences = new TreeSet();
+            for (SAMSequenceRecord dictionaryEntry : trackDict.getSequences())
+                trackSequences.add(dictionaryEntry.getSequenceName());
+            SequenceDictionaryUtils.validateDictionaries(logger, validationExclusionType, trackName, trackDict, "reference", referenceDict);
+        }
+    }
+}
diff --git a/public/java/src/org/broadinstitute/sting/gatk/refdata/tracks/RMDTrackBuilder.java b/public/java/src/org/broadinstitute/sting/gatk/refdata/tracks/RMDTrackBuilder.java
index 46eb79aa7..3b4558579 100644
--- a/public/java/src/org/broadinstitute/sting/gatk/refdata/tracks/RMDTrackBuilder.java
+++ b/public/java/src/org/broadinstitute/sting/gatk/refdata/tracks/RMDTrackBuilder.java
@@ -25,7 +25,6 @@
 package org.broadinstitute.sting.gatk.refdata.tracks;
 
 import net.sf.samtools.SAMSequenceDictionary;
-import net.sf.samtools.SAMSequenceRecord;
 import org.apache.log4j.Logger;
 import org.broad.tribble.FeatureCodec;
 import org.broad.tribble.FeatureSource;
@@ -41,7 +40,6 @@ import org.broadinstitute.sting.gatk.arguments.ValidationExclusion;
 import org.broadinstitute.sting.gatk.refdata.utils.RMDTriplet;
 import org.broadinstitute.sting.gatk.refdata.utils.RMDTriplet.RMDStorageType;
 import org.broadinstitute.sting.utils.GenomeLocParser;
-import org.broadinstitute.sting.utils.SequenceDictionaryUtils;
 import org.broadinstitute.sting.utils.collections.Pair;
 import org.broadinstitute.sting.utils.exceptions.ReviewedStingException;
 import org.broadinstitute.sting.utils.exceptions.UserException;
@@ -52,16 +50,11 @@ import org.broadinstitute.sting.utils.instrumentation.Sizeof;
 import java.io.File;
 import java.io.FileOutputStream;
 import java.io.IOException;
-import java.util.LinkedHashSet;
-import java.util.Map;
-import java.util.Set;
-import java.util.TreeSet;
-
 
 
 /**
- * 
- * @author aaron 
+ *
+ * @author aaron
  *                                           `
  * Class RMDTrackBuilder
  *
@@ -76,9 +69,6 @@ public class RMDTrackBuilder { // extends PluginManager {
     private final static Logger logger = Logger.getLogger(RMDTrackBuilder.class);
     public final static boolean MEASURE_TRIBBLE_QUERY_PERFORMANCE = false;
 
-    // a constant we use for marking sequence dictionary entries in the Tribble index property list
-    public static final String SequenceDictionaryPropertyPredicate = "DICT:";
-
     // private sequence dictionary we use to set our tracks with
     private SAMSequenceDictionary dict = null;
 
@@ -210,13 +200,19 @@ public class RMDTrackBuilder { // extends PluginManager {
                 try { logger.info(String.format("  Index for %s has size in bytes %d", inputFile, Sizeof.getObjectGraphSize(index))); }
                 catch (ReviewedStingException e) { }
 
-                sequenceDictionary = getSequenceDictionaryFromProperties(index);
+                sequenceDictionary = IndexDictionaryUtils.getSequenceDictionaryFromProperties(index);
 
                 // if we don't have a dictionary in the Tribble file, and we've set a dictionary for this builder, set it in the file if they match
                 if (sequenceDictionary.size() == 0 && dict != null) {
                     File indexFile = Tribble.indexFile(inputFile);
-                    setIndexSequenceDictionary(inputFile,index,dict,indexFile,true);
-                    sequenceDictionary = getSequenceDictionaryFromProperties(index);
+                    validateAndUpdateIndexSequenceDictionary(inputFile, index, dict);
+                    try { // re-write the index
+                        writeIndexToDisk(index,indexFile,new FSLockWithShared(indexFile));
+                    } catch (IOException e) {
+                        logger.warn("Unable to update index with the sequence dictionary for file " + indexFile + "; this will not effect your run of the GATK");
+                    }
+
+                    sequenceDictionary = IndexDictionaryUtils.getSequenceDictionaryFromProperties(index);
                 }
 
                 if ( MEASURE_TRIBBLE_QUERY_PERFORMANCE )
@@ -363,96 +359,31 @@ public class RMDTrackBuilder { // extends PluginManager {
         // this can take a while, let them know what we're doing
         logger.info("Creating Tribble index in memory for file " + inputFile);
         Index idx = IndexFactory.createIndex(inputFile, codec, IndexFactory.IndexBalanceApproach.FOR_SEEK_TIME);
-        setIndexSequenceDictionary(inputFile, idx, dict, null, false);
+        validateAndUpdateIndexSequenceDictionary(inputFile, idx, dict);
         return idx;
     }
 
-
-    // ---------------------------------------------------------------------------------------------------------
-    // static functions to work with the sequence dictionaries of indexes
-    // ---------------------------------------------------------------------------------------------------------
-    
-    /**
-     * get the sequence dictionary from the track, if available.  If not, make it from the contig list that is always in the index
-     * @param index the index file to use
-     * @return a SAMSequenceDictionary if available, null if unavailable
-     */
-    public static SAMSequenceDictionary getSequenceDictionaryFromProperties(Index index) {
-        SAMSequenceDictionary dict = new SAMSequenceDictionary();
-        for (Map.Entry entry : index.getProperties().entrySet()) {
-            if (entry.getKey().startsWith(SequenceDictionaryPropertyPredicate))
-                dict.addSequence(new SAMSequenceRecord(entry.getKey().substring(SequenceDictionaryPropertyPredicate.length() , entry.getKey().length()),
-                                 Integer.valueOf(entry.getValue())));
-        }
-        return dict;
-    }
-
-    /**
-     * create the sequence dictionary with the contig list; a backup approach
-     * @param index the index file to use
-     * @param dict the sequence dictionary to add contigs to
-     * @return the filled-in sequence dictionary
-     */
-    private static SAMSequenceDictionary createSequenceDictionaryFromContigList(Index index, SAMSequenceDictionary dict) {
-        LinkedHashSet seqNames = index.getSequenceNames();
-        if (seqNames == null) {
-            return dict;
-        }
-        for (String name : seqNames) {
-            SAMSequenceRecord seq = new SAMSequenceRecord(name, 0);
-            dict.addSequence(seq);
-        }
-        return dict;
-    }
-
     /**
      * set the sequence dictionary of the track.  This function checks that the contig listing of the underlying file is compatible.
      * (that each contig in the index is in the sequence dictionary).
      * @param inputFile for proper error message formatting.
      * @param dict the sequence dictionary
      * @param index the index file
-     * @param indexFile the index file
-     * @param rewriteIndex should we rewrite the index when we're done?
-     *
      */
-    public void setIndexSequenceDictionary(File inputFile, Index index, SAMSequenceDictionary dict, File indexFile, boolean rewriteIndex) {
-        if (dict == null) return;
-
-        SAMSequenceDictionary currentDict = createSequenceDictionaryFromContigList(index, new SAMSequenceDictionary());
+    public void validateAndUpdateIndexSequenceDictionary(final File inputFile, final Index index, final SAMSequenceDictionary dict) {
+        if (dict == null) throw new ReviewedStingException("BUG: dict cannot be null");
 
         // check that every contig in the RMD contig list is at least in the sequence dictionary we're being asked to set
-        validateTrackSequenceDictionary(inputFile.getAbsolutePath(),currentDict,dict);
+        final SAMSequenceDictionary currentDict = IndexDictionaryUtils.createSequenceDictionaryFromContigList(index, new SAMSequenceDictionary());
+        validateTrackSequenceDictionary(inputFile.getAbsolutePath(), currentDict, dict);
 
-        for (SAMSequenceRecord seq : currentDict.getSequences()) {
-            if (dict.getSequence(seq.getSequenceName()) == null)
-                continue;
-            index.addProperty(SequenceDictionaryPropertyPredicate + dict.getSequence(seq.getSequenceName()).getSequenceName(), String.valueOf(dict.getSequence(seq.getSequenceName()).getSequenceLength()));
-        }
-        // re-write the index
-        if (rewriteIndex) try {
-            writeIndexToDisk(index,indexFile,new FSLockWithShared(indexFile));
-        } catch (IOException e) {
-            logger.warn("Unable to update index with the sequence dictionary for file " + indexFile + "; this will not effect your run of the GATK");
-        }
+        // actually update the dictionary in the index
+        IndexDictionaryUtils.setIndexSequenceDictionary(index, dict);
     }
 
-    public void setIndexSequenceDictionary(Index index, SAMSequenceDictionary dict) {
-        for ( SAMSequenceRecord seq : dict.getSequences() ) {
-            final String contig = SequenceDictionaryPropertyPredicate + seq.getSequenceName();
-            final String length = String.valueOf(seq.getSequenceLength());
-            index.addProperty(contig,length);
-        }
-    }
-
-    public void validateTrackSequenceDictionary(String trackName, SAMSequenceDictionary trackDict, SAMSequenceDictionary referenceDict) {
-        // if the sequence dictionary is empty (as well as null which means it doesn't have a dictionary), skip validation
-        if (trackDict == null || trackDict.size() == 0)
-            logger.info("Track " + trackName + " doesn't have a sequence dictionary built in, skipping dictionary validation");
-        else {
-            Set trackSequences = new TreeSet();
-            for (SAMSequenceRecord dictionaryEntry : trackDict.getSequences())
-                trackSequences.add(dictionaryEntry.getSequenceName());
-            SequenceDictionaryUtils.validateDictionaries(logger, validationExclusionType, trackName, trackDict, "reference", referenceDict);
-        }
+    public void validateTrackSequenceDictionary(final String trackName,
+                                                final SAMSequenceDictionary trackDict,
+                                                final SAMSequenceDictionary referenceDict ) {
+        IndexDictionaryUtils.validateTrackSequenceDictionary(trackName, trackDict, referenceDict, validationExclusionType);
     }
 }
diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/Walker.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/Walker.java
index c88c7c3c4..10261112c 100755
--- a/public/java/src/org/broadinstitute/sting/gatk/walkers/Walker.java
+++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/Walker.java
@@ -25,6 +25,7 @@
 
 package org.broadinstitute.sting.gatk.walkers;
 
+import net.sf.samtools.SAMSequenceDictionary;
 import org.apache.log4j.Logger;
 import org.broadinstitute.sting.gatk.CommandLineGATK;
 import org.broadinstitute.sting.gatk.GenomeAnalysisEngine;
@@ -77,6 +78,15 @@ public abstract class Walker {
         return toolkit;
     }
 
+    /**
+     * Gets the master sequence dictionary for this walker
+     * @link GenomeAnalysisEngine.getMasterSequenceDictionary
+     * @return
+     */
+    protected SAMSequenceDictionary getMasterSequenceDictionary() {
+        return getToolkit().getMasterSequenceDictionary();
+    }
+
     /**
      * (conceptual static) method that states whether you want to see reads piling up at a locus
      * that contain a deletion at the locus.
diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/variantutils/LiftoverVariants.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/variantutils/LiftoverVariants.java
index 1c76a21ea..a932d44ed 100755
--- a/public/java/src/org/broadinstitute/sting/gatk/walkers/variantutils/LiftoverVariants.java
+++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/variantutils/LiftoverVariants.java
@@ -99,7 +99,7 @@ public class LiftoverVariants extends RodWalker {
 
 
         final VCFHeader vcfHeader = new VCFHeader(metaData, samples);
-        writer = new StandardVCFWriter(file, false);
+        writer = new StandardVCFWriter(file, getMasterSequenceDictionary(), false);
         writer.writeHeader(vcfHeader);
     }
 
diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/variantutils/RandomlySplitVariants.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/variantutils/RandomlySplitVariants.java
index 1fefd20fc..fa5093839 100755
--- a/public/java/src/org/broadinstitute/sting/gatk/walkers/variantutils/RandomlySplitVariants.java
+++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/variantutils/RandomlySplitVariants.java
@@ -75,7 +75,7 @@ public class RandomlySplitVariants extends RodWalker {
         hInfo.addAll(VCFUtils.getHeaderFields(getToolkit(), inputNames));
 
         vcfWriter1.writeHeader(new VCFHeader(hInfo, samples));
-        vcfWriter2 = new StandardVCFWriter(file2, true);
+        vcfWriter2 = new StandardVCFWriter(file2, getMasterSequenceDictionary(), true);
         vcfWriter2.writeHeader(new VCFHeader(hInfo, samples));
     }
 
diff --git a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/IndexingVCFWriter.java b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/IndexingVCFWriter.java
index 4ae87ddcb..71ec4ce1b 100644
--- a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/IndexingVCFWriter.java
+++ b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/IndexingVCFWriter.java
@@ -24,6 +24,9 @@
 
 package org.broadinstitute.sting.utils.codecs.vcf;
 
+import com.google.java.contract.Ensures;
+import com.google.java.contract.Requires;
+import net.sf.samtools.SAMSequenceDictionary;
 import org.broad.tribble.Tribble;
 import org.broad.tribble.TribbleException;
 import org.broad.tribble.index.DynamicIndexCreator;
@@ -31,7 +34,9 @@ import org.broad.tribble.index.Index;
 import org.broad.tribble.index.IndexFactory;
 import org.broad.tribble.util.LittleEndianOutputStream;
 import org.broad.tribble.util.PositionalStream;
+import org.broadinstitute.sting.gatk.refdata.tracks.IndexDictionaryUtils;
 import org.broadinstitute.sting.utils.exceptions.ReviewedStingException;
+import org.broadinstitute.sting.utils.exceptions.UserException;
 import org.broadinstitute.sting.utils.variantcontext.VariantContext;
 
 import java.io.*;
@@ -41,21 +46,24 @@ import java.io.*;
  */
 public abstract class IndexingVCFWriter implements VCFWriter {
     final private String name;
+    private final SAMSequenceDictionary refDict;
 
-    private File indexFile = null;
     private OutputStream outputStream;
     private PositionalStream positionalStream = null;
     private DynamicIndexCreator indexer = null;
     private LittleEndianOutputStream idxStream = null;
 
-    protected IndexingVCFWriter(String name, File location, OutputStream output, boolean enableOnTheFlyIndexing) {
+    @Requires({"name != null",
+            "! ( location == null && output == null )",
+            "! ( enableOnTheFlyIndexing && location == null )"})
+    protected IndexingVCFWriter(final String name, final File location, final OutputStream output, final SAMSequenceDictionary refDict, final boolean enableOnTheFlyIndexing) {
         outputStream = output;
         this.name = name;
+        this.refDict = refDict;
 
         if ( enableOnTheFlyIndexing ) {
-            indexFile = Tribble.indexFile(location);
             try {
-                idxStream = new LittleEndianOutputStream(new FileOutputStream(indexFile));
+                idxStream = new LittleEndianOutputStream(new FileOutputStream(Tribble.indexFile(location)));
                 //System.out.println("Creating index on the fly for " + location);
                 indexer = new DynamicIndexCreator(IndexFactory.IndexBalanceApproach.FOR_SEEK_TIME);
                 indexer.initialize(location, indexer.defaultBinSize());
@@ -66,15 +74,16 @@ public abstract class IndexingVCFWriter implements VCFWriter {
                 idxStream = null;
                 indexer = null;
                 positionalStream = null;
-                indexFile = null;
             }
         }
     }
 
+    @Ensures("result != null")
     public OutputStream getOutputStream() {
         return outputStream;
     }
 
+    @Ensures("result != null")
     public String getStreamName() {
         return name;
     }
@@ -89,6 +98,7 @@ public abstract class IndexingVCFWriter implements VCFWriter {
         if ( indexer != null ) {
             try {
                 Index index = indexer.finalizeIndex(positionalStream.getPosition());
+                IndexDictionaryUtils.setIndexSequenceDictionary(index, refDict);
                 index.write(idxStream);
                 idxStream.close();
             } catch (IOException e) {
@@ -108,15 +118,27 @@ public abstract class IndexingVCFWriter implements VCFWriter {
             indexer.addFeature(vc, positionalStream.getPosition());
     }
 
-    protected static final String writerName(File location, OutputStream stream) {
+    /**
+     * Returns a reasonable "name" for this writer, to display to the user if something goes wrong
+     *
+     * @param location
+     * @param stream
+     * @return
+     */
+    protected static final String writerName(final File location, final OutputStream stream) {
         return location == null ? stream.toString() : location.getAbsolutePath();
     }
 
-    protected static OutputStream openOutputStream(File location) {
+    /**
+     * Returns a output stream writing to location, or throws a UserException if this fails
+     * @param location
+     * @return
+     */
+    protected static OutputStream openOutputStream(final File location) {
         try {
             return new FileOutputStream(location);
         } catch (FileNotFoundException e) {
-            throw new ReviewedStingException("Unable to create VCF file at location: " + location, e);
+            throw new UserException.CouldNotCreateOutputFile(location, "Unable to create VCF writer", e);
         }
     }
 }
diff --git a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/StandardVCFWriter.java b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/StandardVCFWriter.java
index 7cba5fc3e..0da7a100f 100755
--- a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/StandardVCFWriter.java
+++ b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/StandardVCFWriter.java
@@ -24,6 +24,7 @@
 
 package org.broadinstitute.sting.utils.codecs.vcf;
 
+import net.sf.samtools.SAMSequenceDictionary;
 import org.broad.tribble.Tribble;
 import org.broad.tribble.TribbleException;
 import org.broad.tribble.index.DynamicIndexCreator;
@@ -62,21 +63,12 @@ public class StandardVCFWriter extends IndexingVCFWriter {
      *
      * @param location the file location to write to
      */
-    public StandardVCFWriter(File location) {
-        this(location, openOutputStream(location), true, false);
+    public StandardVCFWriter(final File location, final SAMSequenceDictionary refDict) {
+        this(location, openOutputStream(location), refDict, true, false);
     }
 
-    public StandardVCFWriter(File location, boolean enableOnTheFlyIndexing) {
-        this(location, openOutputStream(location), enableOnTheFlyIndexing, false);
-    }
-
-    /**
-     * create a VCF writer, given a stream to write to
-     *
-     * @param output   the file location to write to
-     */
-    public StandardVCFWriter(OutputStream output) {
-        this(output, false);
+    public StandardVCFWriter(File location, final SAMSequenceDictionary refDict, boolean enableOnTheFlyIndexing) {
+        this(location, openOutputStream(location), refDict, enableOnTheFlyIndexing, false);
     }
 
     /**
@@ -85,12 +77,12 @@ public class StandardVCFWriter extends IndexingVCFWriter {
      * @param output   the file location to write to
      * @param doNotWriteGenotypes   do not write genotypes
      */
-    public StandardVCFWriter(OutputStream output, boolean doNotWriteGenotypes) {
-        this(null, output, false, doNotWriteGenotypes);
+    public StandardVCFWriter(final OutputStream output, final SAMSequenceDictionary refDict, final boolean doNotWriteGenotypes) {
+        this(null, output, refDict, false, doNotWriteGenotypes);
     }
 
-    public StandardVCFWriter(File location, OutputStream output, boolean enableOnTheFlyIndexing, boolean doNotWriteGenotypes) {
-        super(writerName(location, output), location, output, enableOnTheFlyIndexing);
+    public StandardVCFWriter(final File location, final OutputStream output, final SAMSequenceDictionary refDict, final boolean enableOnTheFlyIndexing, boolean doNotWriteGenotypes) {
+        super(writerName(location, output), location, output, refDict, enableOnTheFlyIndexing);
         mWriter = new BufferedWriter(new OutputStreamWriter(getOutputStream())); // todo -- fix buffer size
         this.doNotWriteGenotypes = doNotWriteGenotypes;
     }
diff --git a/public/java/src/org/broadinstitute/sting/utils/gcf/GCFWriter.java b/public/java/src/org/broadinstitute/sting/utils/gcf/GCFWriter.java
index 7ff6e27a2..18fae18c4 100644
--- a/public/java/src/org/broadinstitute/sting/utils/gcf/GCFWriter.java
+++ b/public/java/src/org/broadinstitute/sting/utils/gcf/GCFWriter.java
@@ -24,6 +24,7 @@
 
 package org.broadinstitute.sting.utils.gcf;
 
+import net.sf.samtools.SAMSequenceDictionary;
 import org.broadinstitute.sting.utils.codecs.vcf.IndexingVCFWriter;
 import org.broadinstitute.sting.utils.codecs.vcf.VCFHeader;
 import org.broadinstitute.sting.utils.exceptions.ReviewedStingException;
@@ -52,8 +53,8 @@ public class GCFWriter extends IndexingVCFWriter {
     //
     // --------------------------------------------------------------------------------
 
-    public GCFWriter(File location, boolean enableOnTheFlyIndexing, boolean doNotWriteGenotypes) {
-        super(writerName(location, null), location, null, enableOnTheFlyIndexing);
+    public GCFWriter(final File location, final SAMSequenceDictionary refDict, boolean enableOnTheFlyIndexing, boolean doNotWriteGenotypes) {
+        super(writerName(location, null), location, null, refDict, enableOnTheFlyIndexing);
         this.location = location;
         this.skipGenotypes = doNotWriteGenotypes;
 
diff --git a/public/java/test/org/broadinstitute/sting/WalkerTest.java b/public/java/test/org/broadinstitute/sting/WalkerTest.java
index 386c17659..a1817e3c7 100755
--- a/public/java/test/org/broadinstitute/sting/WalkerTest.java
+++ b/public/java/test/org/broadinstitute/sting/WalkerTest.java
@@ -75,7 +75,7 @@ public class WalkerTest extends BaseTest {
         Index indexFromOutputFile = IndexFactory.createIndex(resultFile, new VCFCodec());
         Index dynamicIndex = IndexFactory.loadIndex(indexFile.getAbsolutePath());
 
-        if ( ! indexFromOutputFile.equalsIgnoreTimestamp(dynamicIndex) ) {
+        if ( ! indexFromOutputFile.equalsIgnoreProperties(dynamicIndex) ) {
             Assert.fail(String.format("Index on disk from indexing on the fly not equal to the index created after the run completed.  FileIndex %s vs. on-the-fly %s%n",
                     indexFromOutputFile.getProperties(),
                     dynamicIndex.getProperties()));
diff --git a/public/java/test/org/broadinstitute/sting/gatk/refdata/tracks/RMDTrackBuilderUnitTest.java b/public/java/test/org/broadinstitute/sting/gatk/refdata/tracks/RMDTrackBuilderUnitTest.java
index ae218e898..724c343e4 100644
--- a/public/java/test/org/broadinstitute/sting/gatk/refdata/tracks/RMDTrackBuilderUnitTest.java
+++ b/public/java/test/org/broadinstitute/sting/gatk/refdata/tracks/RMDTrackBuilderUnitTest.java
@@ -29,7 +29,6 @@ import net.sf.picard.reference.IndexedFastaSequenceFile;
 import net.sf.samtools.SAMSequenceDictionary;
 import org.broad.tribble.Tribble;
 import org.broad.tribble.index.Index;
-import org.broadinstitute.sting.gatk.refdata.tracks.RMDTrackBuilder;
 import org.broadinstitute.sting.utils.codecs.vcf.VCF3Codec;
 import org.broadinstitute.sting.utils.codecs.vcf.VCFCodec;
 import org.broadinstitute.sting.utils.exceptions.UserException;
@@ -45,7 +44,6 @@ import org.testng.annotations.Test;
 
 import java.io.*;
 import java.nio.channels.FileChannel;
-import java.util.Map;
 
 
 /**
@@ -164,7 +162,7 @@ public class RMDTrackBuilderUnitTest extends BaseTest {
         try {
             Index idx = builder.loadIndex(vcfFile, new VCFCodec());
             // catch any exception; this call should pass correctly
-            SAMSequenceDictionary dict =  RMDTrackBuilder.getSequenceDictionaryFromProperties(idx);
+            SAMSequenceDictionary dict =  IndexDictionaryUtils.getSequenceDictionaryFromProperties(idx);
         } catch (IOException e) {
             e.printStackTrace();
             Assert.fail("IO exception unexpected" + e.getMessage());
diff --git a/public/java/test/org/broadinstitute/sting/utils/codecs/vcf/IndexFactoryUnitTest.java b/public/java/test/org/broadinstitute/sting/utils/codecs/vcf/IndexFactoryUnitTest.java
index 1809ab778..55bd4783b 100755
--- a/public/java/test/org/broadinstitute/sting/utils/codecs/vcf/IndexFactoryUnitTest.java
+++ b/public/java/test/org/broadinstitute/sting/utils/codecs/vcf/IndexFactoryUnitTest.java
@@ -1,27 +1,45 @@
 package org.broadinstitute.sting.utils.codecs.vcf;
 
+import net.sf.samtools.SAMSequenceDictionary;
 import org.broad.tribble.Tribble;
 import org.broad.tribble.index.*;
 import org.broad.tribble.iterators.CloseableTribbleIterator;
 import org.broad.tribble.source.BasicFeatureSource;
+import org.broadinstitute.sting.BaseTest;
 import org.broadinstitute.sting.WalkerTest;
+import org.broadinstitute.sting.utils.exceptions.UserException;
+import org.broadinstitute.sting.utils.fasta.CachingIndexedFastaSequenceFile;
 import org.broadinstitute.sting.utils.variantcontext.VariantContext;
 import org.testng.Assert;
+import org.testng.annotations.BeforeTest;
 import org.testng.annotations.Test;
 
 import java.io.File;
+import java.io.FileNotFoundException;
 import java.io.IOException;
 import java.util.*;
 
 /**
  * tests out the various functions in the index factory class
  */
-public class IndexFactoryUnitTest {
+public class IndexFactoryUnitTest extends BaseTest {
 
     File inputFile = new File("public/testdata/HiSeq.10000.vcf");
     File outputFile = new File("public/testdata/onTheFlyOutputTest.vcf");
     File outputFileIndex = Tribble.indexFile(outputFile);
 
+    private SAMSequenceDictionary dict;
+
+    @BeforeTest
+    public void setup() {
+        try {
+            dict = new CachingIndexedFastaSequenceFile(new File(b37KGReference)).getSequenceDictionary();
+        }
+        catch(FileNotFoundException ex) {
+            throw new UserException.CouldNotReadInputFile(b37KGReference,ex);
+        }
+    }
+
     //
     // test out scoring the indexes
     //
@@ -37,7 +55,7 @@ public class IndexFactoryUnitTest {
             BasicFeatureSource source = new BasicFeatureSource(inputFile.getAbsolutePath(), indexFromInputFile, new VCFCodec());
 
             int counter = 0;
-            VCFWriter writer = new StandardVCFWriter(outputFile);
+            VCFWriter writer = new StandardVCFWriter(outputFile, dict);
             writer.writeHeader((VCFHeader)source.getHeader());
             CloseableTribbleIterator it = source.iterator();
             while (it.hasNext() && (counter++ < maxRecords || maxRecords == -1) ) {
diff --git a/public/java/test/org/broadinstitute/sting/utils/genotype/vcf/VCFWriterUnitTest.java b/public/java/test/org/broadinstitute/sting/utils/genotype/vcf/VCFWriterUnitTest.java
index e3a926fb9..a8e6593b1 100644
--- a/public/java/test/org/broadinstitute/sting/utils/genotype/vcf/VCFWriterUnitTest.java
+++ b/public/java/test/org/broadinstitute/sting/utils/genotype/vcf/VCFWriterUnitTest.java
@@ -38,12 +38,13 @@ public class VCFWriterUnitTest extends BaseTest {
     private Set additionalColumns = new HashSet();
     private File fakeVCFFile = new File("FAKEVCFFILEFORTESTING.vcf");
     private GenomeLocParser genomeLocParser;
+    private IndexedFastaSequenceFile seq;
 
     @BeforeClass
     public void beforeTests() {
         File referenceFile = new File(hg18Reference);
         try {
-            IndexedFastaSequenceFile seq = new CachingIndexedFastaSequenceFile(referenceFile);
+            seq = new CachingIndexedFastaSequenceFile(referenceFile);
             genomeLocParser = new GenomeLocParser(seq);
         }
         catch(FileNotFoundException ex) {
@@ -55,7 +56,7 @@ public class VCFWriterUnitTest extends BaseTest {
     @Test
     public void testBasicWriteAndRead() {
         VCFHeader header = createFakeHeader(metaData,additionalColumns);
-        VCFWriter writer = new StandardVCFWriter(fakeVCFFile);
+        VCFWriter writer = new StandardVCFWriter(fakeVCFFile, seq.getSequenceDictionary());
         writer.writeHeader(header);
         writer.add(createVC(header));
         writer.add(createVC(header));

From 08ffb18b96b1d22f14c488cd71a1d02b5490f6a1 Mon Sep 17 00:00:00 2001
From: Mauricio Carneiro 
Date: Tue, 20 Sep 2011 11:02:51 -0400
Subject: [PATCH 23/41] Renaming datasets in the MDCP

Making dataset names and files generated by the MDCP more uniform.
---
 .../MethodsDevelopmentCallingPipeline.scala   | 40 ++++++++++++-------
 1 file changed, 26 insertions(+), 14 deletions(-)

diff --git a/public/scala/qscript/org/broadinstitute/sting/queue/qscripts/MethodsDevelopmentCallingPipeline.scala b/public/scala/qscript/org/broadinstitute/sting/queue/qscripts/MethodsDevelopmentCallingPipeline.scala
index 80bfe03d1..637d3edca 100755
--- a/public/scala/qscript/org/broadinstitute/sting/queue/qscripts/MethodsDevelopmentCallingPipeline.scala
+++ b/public/scala/qscript/org/broadinstitute/sting/queue/qscripts/MethodsDevelopmentCallingPipeline.scala
@@ -98,40 +98,52 @@ class MethodsDevelopmentCallingPipeline extends QScript {
   // BUGBUG: We no longer support b36/hg18 because several of the necessary files aren't available aligned to those references
 
   val targetDataSets: Map[String, Target] = Map(
-    "HiSeq" -> new Target("NA12878.HiSeq", hg18, dbSNP_hg18_129, hapmap_hg18,
+    "NA12878_wgs_b37" -> new Target("NA12878.HiSeq.WGS.b37", hg19, dbSNP_b37, hapmap_b37, indelMask_b37,
+              new File("/humgen/gsa-hpprojects/NA12878Collection/bams/NA12878.HiSeq.WGS.bwa.cleaned.recal.hg19.bam"),
+              new File("/humgen/gsa-hpprojects/dev/carneiro/hiseq19/analysis/snps/NA12878.HiSeq19.filtered.vcf"),
+              "/humgen/1kg/processing/pipeline_test_bams/whole_genome_chunked.noChrY.hg19.intervals", 2.14, 99.0, !lowPass, !exome, 1),
+    "NA12878_wgs_decoy" -> new Target("NA12878.HiSeq.WGS.b37_decoy", b37_decoy, dbSNP_b37, hapmap_b37, indelMask_b37,
+              new File("/humgen/gsa-hpprojects/NA12878Collection/bams/CEUTrio.HiSeq.WGS.b37_decoy.NA12878.clean.dedup.recal.bam"),
+              new File("/humgen/gsa-hpprojects/dev/carneiro/hiseq19/analysis/snps/NA12878.HiSeq19.filtered.vcf"),          // ** THIS GOLD STANDARD NEEDS TO BE CORRECTED **
+              "/humgen/1kg/processing/pipeline_test_bams/whole_genome_chunked.noChrY.hg19.intervals", 2.14, 99.0, !lowPass, !exome, 1),
+    "NA12878_wgs_hg18" -> new Target("NA12878.HiSeq.WGS.hg18", hg18, dbSNP_hg18_129, hapmap_hg18,
               "/humgen/gsa-hpprojects/dev/depristo/oneOffProjects/1000GenomesProcessingPaper/wgs.v13/HiSeq.WGS.cleaned.indels.10.mask",
               new File("/humgen/gsa-hpprojects/NA12878Collection/bams/NA12878.HiSeq.WGS.bwa.cleaned.recal.bam"),
               new File("/home/radon01/depristo/work/oneOffProjects/1000GenomesProcessingPaper/wgs.v13/HiSeq.WGS.cleaned.ug.snpfiltered.indelfiltered.vcf"),
               "/humgen/1kg/processing/pipeline_test_bams/whole_genome_chunked.hg18.intervals", 2.14, 99.0, !lowPass, !exome, 1),
-    "HiSeq19" -> new Target("NA12878.HiSeq19", hg19, dbSNP_b37, hapmap_b37, indelMask_b37,
-              new File("/humgen/gsa-hpprojects/NA12878Collection/bams/NA12878.HiSeq.WGS.bwa.cleaned.recal.hg19.bam"),
-              new File("/humgen/gsa-hpprojects/dev/carneiro/hiseq19/analysis/snps/NA12878.HiSeq19.filtered.vcf"),
-              "/humgen/1kg/processing/pipeline_test_bams/whole_genome_chunked.noChrY.hg19.intervals", 2.14, 99.0, !lowPass, !exome, 1),
-    "GA2hg19" -> new Target("NA12878.GA2.hg19", hg19, dbSNP_b37, hapmap_b37, indelMask_b37,
-              new File("/humgen/gsa-hpprojects/NA12878Collection/bams/NA12878.GA2.WGS.bwa.cleaned.hg19.bam"),
-              new File("/humgen/gsa-hpprojects/dev/carneiro/hiseq19/analysis/snps/NA12878.GA2.hg19.filtered.vcf"),
-              "/humgen/1kg/processing/pipeline_test_bams/whole_genome_chunked.hg19.intervals", 2.14, 99.0, !lowPass, !exome, 1),
-    "WEx" -> new Target("NA12878.WEx", hg18, dbSNP_hg18_129, hapmap_hg18,
+    "NA12878_wex_b37" -> new Target("NA12878.HiSeq.WEx.b37", hg19, dbSNP_b37, hapmap_b37, indelMask_b37,
+              new File("/seq/picard_aggregation/C339/NA12878/v3/NA12878.bam"),
+              new File("/humgen/gsa-hpprojects/dev/carneiro/trio/analysis/snps/CEUTrio.WEx.filtered.vcf"),                  // ** THIS GOLD STANDARD NEEDS TO BE CORRECTED **
+              "/seq/references/HybSelOligos/whole_exome_agilent_1.1_refseq_plus_3_boosters/whole_exome_agilent_1.1_refseq_plus_3_boosters.Homo_sapiens_assembly19.targets.interval_list", 3.3, 98.0, !lowPass, exome, 1),
+    "NA12878_wex_hg18" -> new Target("NA12878.HiSeq.WEx.hg18", hg18, dbSNP_hg18_129, hapmap_hg18,
               "/humgen/gsa-hpprojects/dev/depristo/oneOffProjects/1000GenomesProcessingPaper/wgs.v13/GA2.WEx.cleaned.indels.10.mask",
               new File("/humgen/gsa-hpprojects/NA12878Collection/bams/NA12878.WEx.cleaned.recal.bam"),
               new File("/home/radon01/depristo/work/oneOffProjects/1000GenomesProcessingPaper/wgs.v13/GA2.WEx.cleaned.ug.snpfiltered.indelfiltered.vcf"),
               "/seq/references/HybSelOligos/whole_exome_agilent_1.1_refseq_plus_3_boosters/whole_exome_agilent_1.1_refseq_plus_3_boosters.targets.interval_list", 3.3, 98.0, !lowPass, exome, 1),
-    "WExTrio" -> new Target("CEUTrio.WEx", hg19, dbSNP_b37, hapmap_b37, indelMask_b37,
+    "NA12878_wex_decoy" -> new Target("NA12878.HiSeq.WEx.b37_decoy", b37_decoy, dbSNP_b37, hapmap_b37, indelMask_b37,
+              new File("/humgen/gsa-hpprojects/NA12878Collection/bams/CEUTrio.HiSeq.WEx.b37_decoy.NA12878.clean.dedup.recal.bam"),
+              new File("/humgen/gsa-hpprojects/dev/carneiro/trio/analysis/snps/CEUTrio.WEx.filtered.vcf"),                  // ** THIS GOLD STANDARD NEEDS TO BE CORRECTED **
+              "/seq/references/HybSelOligos/whole_exome_agilent_1.1_refseq_plus_3_boosters/whole_exome_agilent_1.1_refseq_plus_3_boosters.Homo_sapiens_assembly19.targets.interval_list", 3.3, 98.0, !lowPass, exome, 1),
+    "CEUTrio_wex_b37" -> new Target("CEUTrio.HiSeq.WEx.b37", hg19, dbSNP_b37, hapmap_b37, indelMask_b37,
               new File("/humgen/gsa-hpprojects/NA12878Collection/bams/CEUTrio.HiSeq.WEx.bwa.cleaned.recal.bam"),
               new File("/humgen/gsa-hpprojects/dev/carneiro/trio/analysis/snps/CEUTrio.WEx.filtered.vcf"),
               "/seq/references/HybSelOligos/whole_exome_agilent_1.1_refseq_plus_3_boosters/whole_exome_agilent_1.1_refseq_plus_3_boosters.Homo_sapiens_assembly19.targets.interval_list", 3.3, 98.0, !lowPass, exome, 3),
-    "WGSTrio" -> new Target("CEUTrio.WGS", hg19, dbSNP_b37, hapmap_b37, indelMask_b37,
+    "CEUTrio_wgs_b37" -> new Target("CEUTrio.HiSeq.WGS.b37", hg19, dbSNP_b37, hapmap_b37, indelMask_b37,
               new File("/humgen/gsa-hpprojects/NA12878Collection/bams/CEUTrio.HiSeq.WGS.bwa.cleaned.recal.bam"),
               new File("/humgen/gsa-hpprojects/dev/carneiro/trio/analysis/snps/CEUTrio.WEx.filtered.vcf"),                  // ** THIS GOLD STANDARD NEEDS TO BE CORRECTED **
               "/humgen/1kg/processing/pipeline_test_bams/whole_genome_chunked.hg19.intervals", 2.3, 99.0, !lowPass, !exome, 3),
-    "WExTrioDecoy" -> new Target("CEUTrio.HiSeq.WEx.b37_decoy", b37_decoy, dbSNP_b37, hapmap_b37, indelMask_b37,
+    "CEUTrio_wex_decoy" -> new Target("CEUTrio.HiSeq.WEx.b37_decoy", b37_decoy, dbSNP_b37, hapmap_b37, indelMask_b37,
               new File("/humgen/gsa-hpprojects/NA12878Collection/bams/CEUTrio.HiSeq.WEx.b37_decoy.list"),
               new File("/humgen/gsa-hpprojects/dev/carneiro/trio/analysis/snps/CEUTrio.WEx.filtered.vcf"),                  // ** THIS GOLD STANDARD NEEDS TO BE CORRECTED **
               "/seq/references/HybSelOligos/whole_exome_agilent_1.1_refseq_plus_3_boosters/whole_exome_agilent_1.1_refseq_plus_3_boosters.Homo_sapiens_assembly19.targets.interval_list", 3.3, 98.0, !lowPass, exome, 3),
-    "WGSTrioDecoy" -> new Target("CEUTrio.HiSeq.WGS.b37_decoy", b37_decoy, dbSNP_b37, hapmap_b37, indelMask_b37,
+    "CEUTrio_wgs_decoy" -> new Target("CEUTrio.HiSeq.WGS.b37_decoy", b37_decoy, dbSNP_b37, hapmap_b37, indelMask_b37,
               new File("/humgen/gsa-hpprojects/NA12878Collection/bams/CEUTrio.HiSeq.WGS.b37_decoy.list"),
               new File("/humgen/gsa-hpprojects/dev/carneiro/trio/analysis/snps/CEUTrio.WEx.filtered.vcf"),                  // ** THIS GOLD STANDARD NEEDS TO BE CORRECTED **
               "/humgen/1kg/processing/pipeline_test_bams/whole_genome_chunked.hg19.intervals", 2.3, 99.0, !lowPass, !exome, 3),
+    "GA2hg19" -> new Target("NA12878.GA2.hg19", hg19, dbSNP_b37, hapmap_b37, indelMask_b37,
+              new File("/humgen/gsa-hpprojects/NA12878Collection/bams/NA12878.GA2.WGS.bwa.cleaned.hg19.bam"),
+              new File("/humgen/gsa-hpprojects/dev/carneiro/hiseq19/analysis/snps/NA12878.GA2.hg19.filtered.vcf"),
+              "/humgen/1kg/processing/pipeline_test_bams/whole_genome_chunked.hg19.intervals", 2.14, 99.0, !lowPass, !exome, 1),
     "FIN" -> new Target("FIN", b37, dbSNP_b37, hapmap_b37, indelMask_b37,
               new File("/humgen/1kg/processing/pipeline_test_bams/FIN.79sample.Nov2010.chr20.bam"),
               new File("/humgen/gsa-hpprojects/dev/data/AugChr20Calls_v4_3state/ALL.august.v4.chr20.filtered.vcf"),         // ** THIS GOLD STANDARD NEEDS TO BE CORRECTED **

From a1b4cafe7a63ecbeea9b06a94445f6bbf925d5e1 Mon Sep 17 00:00:00 2001
From: Mark DePristo 
Date: Tue, 20 Sep 2011 13:59:59 -0400
Subject: [PATCH 24/41] Bug fix for NPE when timer wasn't initialized

---
 .../broadinstitute/sting/gatk/traversals/TraversalEngine.java   | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/public/java/src/org/broadinstitute/sting/gatk/traversals/TraversalEngine.java b/public/java/src/org/broadinstitute/sting/gatk/traversals/TraversalEngine.java
index 27fd173cb..c6321e2ad 100755
--- a/public/java/src/org/broadinstitute/sting/gatk/traversals/TraversalEngine.java
+++ b/public/java/src/org/broadinstitute/sting/gatk/traversals/TraversalEngine.java
@@ -358,7 +358,7 @@ public abstract class TraversalEngine,Provide
     public void printOnTraversalDone() {
         printProgress(null, null, true);
 
-        final double elapsed = timer.getElapsedTime();
+        final double elapsed = timer == null ? 0 : timer.getElapsedTime();
 
         ReadMetrics cumulativeMetrics = engine.getCumulativeMetrics();        
 

From 827c942c8027d7888b29ddff8399834b7bcf94e4 Mon Sep 17 00:00:00 2001
From: Mark DePristo 
Date: Tue, 20 Sep 2011 14:01:14 -0400
Subject: [PATCH 25/41] Rev tribble

---
 .../{tribble-24.jar => tribble-25.jar}        | Bin 299210 -> 305986 bytes
 .../{tribble-24.xml => tribble-25.xml}        |   2 +-
 2 files changed, 1 insertion(+), 1 deletion(-)
 rename settings/repository/org.broad/{tribble-24.jar => tribble-25.jar} (89%)
 rename settings/repository/org.broad/{tribble-24.xml => tribble-25.xml} (51%)

diff --git a/settings/repository/org.broad/tribble-24.jar b/settings/repository/org.broad/tribble-25.jar
similarity index 89%
rename from settings/repository/org.broad/tribble-24.jar
rename to settings/repository/org.broad/tribble-25.jar
index b1c39e60a14ce895e515cadb07c356d6d428bbc4..6d467ba3fdd6e6285e8244a87aefa4a0d232cfe1 100644
GIT binary patch
delta 11585
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z;7tR4K-XfWdO7^9j7rl4?$%O7{#TR;y~8Q^w0#E`%{4dw%f+`<{H>8pA1Cr#Y4j1Ng%%*T)-gSKtzyIxDu1~7*H?pL>M=c>O>$?I?Gb>dmFvBr?q$~;D)`&!U%F7H~)Ex
zE;LAoKdq%oQm?TDBzYe=-A#MQ@Js1b&FWYD@fLgjY0_KmqevD_pV2}>%IP4hnqm-(
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diff --git a/settings/repository/org.broad/tribble-24.xml b/settings/repository/org.broad/tribble-25.xml
similarity index 51%
rename from settings/repository/org.broad/tribble-24.xml
rename to settings/repository/org.broad/tribble-25.xml
index 9b2b967f8..ed7a1fd69 100644
--- a/settings/repository/org.broad/tribble-24.xml
+++ b/settings/repository/org.broad/tribble-25.xml
@@ -1,3 +1,3 @@
 
-    
+    
 

From bffd3cca6fe26fe31a6fa5a33745dcaee0139c56 Mon Sep 17 00:00:00 2001
From: Mark DePristo 
Date: Tue, 20 Sep 2011 15:07:06 -0400
Subject: [PATCH 27/41] Bug fix for reduced read; only adds regular bases for
 calculation

-- No longer passes on deletions for genotyping
---
 .../walkers/genotyper/DiploidSNPGenotypeLikelihoods.java   | 7 +++++--
 1 file changed, 5 insertions(+), 2 deletions(-)

diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/genotyper/DiploidSNPGenotypeLikelihoods.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/genotyper/DiploidSNPGenotypeLikelihoods.java
index 5f6865d04..ec180f0cd 100755
--- a/public/java/src/org/broadinstitute/sting/gatk/walkers/genotyper/DiploidSNPGenotypeLikelihoods.java
+++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/genotyper/DiploidSNPGenotypeLikelihoods.java
@@ -276,8 +276,11 @@ public class DiploidSNPGenotypeLikelihoods implements Cloneable {
         if ( elt.isReducedRead() ) {
             // reduced read representation
             byte qual = elt.getReducedQual();
-            add(obsBase, qual, (byte)0, (byte)0, elt.getReducedCount()); // fast calculation of n identical likelihoods
-            return elt.getReducedCount(); // we added nObs bases here
+            if ( BaseUtils.isRegularBase( elt.getBase() )) {
+                add(obsBase, qual, (byte)0, (byte)0, elt.getReducedCount()); // fast calculation of n identical likelihoods
+                return elt.getReducedCount(); // we added nObs bases here
+            } else // odd bases or deletions => don't use them
+                return 0;
         } else {
             byte qual = qualToUse(elt, ignoreBadBases, capBaseQualsAtMappingQual, minBaseQual);
             return qual > 0 ? add(obsBase, qual, (byte)0, (byte)0, 1) : 0;

From d9ea764611c0e6b63e8b6e2022fafc52ee56bea9 Mon Sep 17 00:00:00 2001
From: David Roazen 
Date: Tue, 20 Sep 2011 15:22:27 -0400
Subject: [PATCH 28/41] SnpEff annotator now adds OriginalSnpEffVersion and
 OriginalSnpEffCmd lines to the header of the VCF output file.

This change is urgently required for production, which is why it's going into Stable+Unstable
instead of just Unstable.

The keys for the SnpEff version and command header lines in the VCF file output by
VariantAnnotator (OriginalSnpEffVersion and OriginalSnpEffCmd) are intentionally
different from the keys for those same lines in the SnpEff output file (SnpEffVersion
and SnpEffCmd), so that output files from VariantAnnotator won't be confused
with output files from SnpEff itself.
---
 .../sting/gatk/walkers/annotator/SnpEff.java  | 49 +++++++++++++++----
 .../walkers/annotator/VariantAnnotator.java   |  4 +-
 .../annotator/VariantAnnotatorEngine.java     |  6 +--
 .../VariantAnnotatorAnnotation.java           |  4 +-
 .../VariantAnnotatorIntegrationTest.java      |  2 +-
 5 files changed, 48 insertions(+), 17 deletions(-)

diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/annotator/SnpEff.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/annotator/SnpEff.java
index 4ead77506..8f99c6118 100644
--- a/public/java/src/org/broadinstitute/sting/gatk/walkers/annotator/SnpEff.java
+++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/annotator/SnpEff.java
@@ -58,6 +58,13 @@ public class SnpEff extends InfoFieldAnnotation implements ExperimentalAnnotatio
     // lacking a SnpEff version number in the VCF header:
     public static final String[] SUPPORTED_SNPEFF_VERSIONS = { "2.0.2" };
     public static final String SNPEFF_VCF_HEADER_VERSION_LINE_KEY = "SnpEffVersion";
+    public static final String SNPEFF_VCF_HEADER_COMMAND_LINE_KEY = "SnpEffCmd";
+
+    // When we write the SnpEff version number and command line to the output VCF, we change
+    // the key name slightly so that the output VCF won't be confused in the future for an
+    // output file produced by SnpEff directly:
+    public static final String OUTPUT_VCF_HEADER_VERSION_LINE_KEY = "Original" + SNPEFF_VCF_HEADER_VERSION_LINE_KEY;
+    public static final String OUTPUT_VCF_HEADER_COMMAND_LINE_KEY = "Original" + SNPEFF_VCF_HEADER_COMMAND_LINE_KEY;
 
     // SnpEff aggregates all effects (and effect metadata) together into a single INFO
     // field annotation with the key EFF:
@@ -165,10 +172,26 @@ public class SnpEff extends InfoFieldAnnotation implements ExperimentalAnnotatio
         UNKNOWN
     }
 
-
-    public void initialize ( AnnotatorCompatibleWalker walker, GenomeAnalysisEngine toolkit ) {
+    public void initialize ( AnnotatorCompatibleWalker walker, GenomeAnalysisEngine toolkit, Set headerLines ) {
+        // Make sure that we actually have a valid SnpEff rod binding (just in case the user specified -A SnpEff
+        // without providing a SnpEff rod via --snpEffFile):
         validateRodBinding(walker.getSnpEffRodBinding());
-        checkSnpEffVersion(walker, toolkit);
+        RodBinding snpEffRodBinding = walker.getSnpEffRodBinding();
+
+        // Make sure that the SnpEff version number and command-line header lines are present in the VCF header of
+        // the SnpEff rod, and that the file was generated by a supported version of SnpEff:
+        VCFHeader snpEffVCFHeader = VCFUtils.getVCFHeadersFromRods(toolkit, Arrays.asList(snpEffRodBinding.getName())).get(snpEffRodBinding.getName());
+        VCFHeaderLine snpEffVersionLine = snpEffVCFHeader.getOtherHeaderLine(SNPEFF_VCF_HEADER_VERSION_LINE_KEY);
+        VCFHeaderLine snpEffCommandLine = snpEffVCFHeader.getOtherHeaderLine(SNPEFF_VCF_HEADER_COMMAND_LINE_KEY);
+
+        checkSnpEffVersion(snpEffVersionLine);
+        checkSnpEffCommandLine(snpEffCommandLine);
+
+        // If everything looks ok, add the SnpEff version number and command-line header lines to the
+        // header of the VCF output file, changing the key names so that our output file won't be
+        // mistaken in the future for a SnpEff output file:
+        headerLines.add(new VCFHeaderLine(OUTPUT_VCF_HEADER_VERSION_LINE_KEY, snpEffVersionLine.getValue()));
+        headerLines.add(new VCFHeaderLine(OUTPUT_VCF_HEADER_COMMAND_LINE_KEY, snpEffCommandLine.getValue()));
     }
 
     public Map annotate ( RefMetaDataTracker tracker, AnnotatorCompatibleWalker walker, ReferenceContext ref, Map stratifiedContexts, VariantContext vc ) {
@@ -204,12 +227,7 @@ public class SnpEff extends InfoFieldAnnotation implements ExperimentalAnnotatio
         }
     }
 
-    private void checkSnpEffVersion ( AnnotatorCompatibleWalker walker, GenomeAnalysisEngine toolkit ) {
-        RodBinding snpEffRodBinding = walker.getSnpEffRodBinding();
-
-        VCFHeader snpEffVCFHeader = VCFUtils.getVCFHeadersFromRods(toolkit, Arrays.asList(snpEffRodBinding.getName())).get(snpEffRodBinding.getName());
-        VCFHeaderLine snpEffVersionLine = snpEffVCFHeader.getOtherHeaderLine(SNPEFF_VCF_HEADER_VERSION_LINE_KEY);
-
+    private void checkSnpEffVersion ( VCFHeaderLine snpEffVersionLine ) {
         if ( snpEffVersionLine == null || snpEffVersionLine.getValue() == null || snpEffVersionLine.getValue().trim().length() == 0 ) {
             throw new UserException("Could not find a " + SNPEFF_VCF_HEADER_VERSION_LINE_KEY + " entry in the VCF header for the SnpEff " +
                                     "input file, and so could not verify that the file was generated by a supported version of SnpEff (" +
@@ -224,6 +242,14 @@ public class SnpEff extends InfoFieldAnnotation implements ExperimentalAnnotatio
         }
     }
 
+    private void checkSnpEffCommandLine ( VCFHeaderLine snpEffCommandLine ) {
+        if ( snpEffCommandLine == null || snpEffCommandLine.getValue() == null || snpEffCommandLine.getValue().trim().length() == 0 ) {
+            throw new UserException("Could not find a " + SNPEFF_VCF_HEADER_COMMAND_LINE_KEY + " entry in the VCF header for the SnpEff " +
+                                    "input file, which should be added by all supported versions of SnpEff (" +
+                                    Arrays.toString(SUPPORTED_SNPEFF_VERSIONS) + ")");
+        }
+    }
+
     private boolean isSupportedSnpEffVersion ( String versionString ) {
         for ( String supportedVersion : SUPPORTED_SNPEFF_VERSIONS ) {
             if ( supportedVersion.equals(versionString) ) {
@@ -248,10 +274,13 @@ public class SnpEff extends InfoFieldAnnotation implements ExperimentalAnnotatio
         List parsedEffects = new ArrayList();
 
         Object effectFieldValue = snpEffRecord.getAttribute(SNPEFF_INFO_FIELD_KEY);
-        List individualEffects;
+        if ( effectFieldValue == null ) {
+            return parsedEffects;
+        }
 
         // The VCF codec stores multi-valued fields as a List, and single-valued fields as a String.
         // We can have either in the case of SnpEff, since there may be one or more than one effect in this record.
+        List individualEffects;
         if ( effectFieldValue instanceof List ) {
             individualEffects = (List)effectFieldValue;
         }
diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/annotator/VariantAnnotator.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/annotator/VariantAnnotator.java
index fb3dbc3cf..f6a1c4f31 100755
--- a/public/java/src/org/broadinstitute/sting/gatk/walkers/annotator/VariantAnnotator.java
+++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/annotator/VariantAnnotator.java
@@ -208,8 +208,6 @@ public class VariantAnnotator extends RodWalker implements Ann
             engine = new VariantAnnotatorEngine(annotationGroupsToUse, annotationsToUse, this, getToolkit());
         engine.initializeExpressions(expressionsToUse);
 
-        engine.invokeAnnotationInitializationMethods();
-
         // setup the header fields
         // note that if any of the definitions conflict with our new ones, then we want to overwrite the old ones
         Set hInfo = new HashSet();
@@ -219,6 +217,8 @@ public class VariantAnnotator extends RodWalker implements Ann
                 hInfo.add(line);
         }
 
+        engine.invokeAnnotationInitializationMethods(hInfo);
+
         VCFHeader vcfHeader = new VCFHeader(hInfo, samples);
         vcfWriter.writeHeader(vcfHeader);
 
diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/annotator/VariantAnnotatorEngine.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/annotator/VariantAnnotatorEngine.java
index 68cd07803..e5effe6d8 100755
--- a/public/java/src/org/broadinstitute/sting/gatk/walkers/annotator/VariantAnnotatorEngine.java
+++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/annotator/VariantAnnotatorEngine.java
@@ -114,13 +114,13 @@ public class VariantAnnotatorEngine {
             dbAnnotations.put(rod, rod.getName());
     }
 
-    public void invokeAnnotationInitializationMethods() {
+    public void invokeAnnotationInitializationMethods( Set headerLines ) {
         for ( VariantAnnotatorAnnotation annotation : requestedInfoAnnotations ) {
-            annotation.initialize(walker, toolkit);
+            annotation.initialize(walker, toolkit, headerLines);
         }
 
         for ( VariantAnnotatorAnnotation annotation : requestedGenotypeAnnotations ) {
-            annotation.initialize(walker, toolkit);
+            annotation.initialize(walker, toolkit, headerLines);
         }
     }
 
diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/annotator/interfaces/VariantAnnotatorAnnotation.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/annotator/interfaces/VariantAnnotatorAnnotation.java
index 160a3d258..521f89016 100644
--- a/public/java/src/org/broadinstitute/sting/gatk/walkers/annotator/interfaces/VariantAnnotatorAnnotation.java
+++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/annotator/interfaces/VariantAnnotatorAnnotation.java
@@ -25,9 +25,11 @@
 package org.broadinstitute.sting.gatk.walkers.annotator.interfaces;
 
 import org.broadinstitute.sting.gatk.GenomeAnalysisEngine;
+import org.broadinstitute.sting.utils.codecs.vcf.VCFHeaderLine;
 import org.broadinstitute.sting.utils.help.DocumentedGATKFeature;
 
 import java.util.List;
+import java.util.Set;
 
 @DocumentedGATKFeature(enable = true, groupName = "VariantAnnotator annotations", summary = "VariantAnnotator annotations")
 public abstract class VariantAnnotatorAnnotation {
@@ -35,5 +37,5 @@ public abstract class VariantAnnotatorAnnotation {
     public abstract List getKeyNames();
 
     // initialization method (optional for subclasses, and therefore non-abstract)
-    public void initialize ( AnnotatorCompatibleWalker walker, GenomeAnalysisEngine toolkit ) { }
+    public void initialize ( AnnotatorCompatibleWalker walker, GenomeAnalysisEngine toolkit, Set headerLines ) { }
 }
\ No newline at end of file
diff --git a/public/java/test/org/broadinstitute/sting/gatk/walkers/annotator/VariantAnnotatorIntegrationTest.java b/public/java/test/org/broadinstitute/sting/gatk/walkers/annotator/VariantAnnotatorIntegrationTest.java
index 08baae7a7..2c06c6b7f 100755
--- a/public/java/test/org/broadinstitute/sting/gatk/walkers/annotator/VariantAnnotatorIntegrationTest.java
+++ b/public/java/test/org/broadinstitute/sting/gatk/walkers/annotator/VariantAnnotatorIntegrationTest.java
@@ -134,7 +134,7 @@ public class VariantAnnotatorIntegrationTest extends WalkerTest {
             validationDataLocation + "1kg_exomes_unfiltered.AFR.unfiltered.vcf --snpEffFile  " + validationDataLocation +
             "snpEff.AFR.unfiltered.vcf -L 1:1-1,500,000",
             1,
-            Arrays.asList("486fc6a5ca1819f5ab180d5d72b1ebc9")
+            Arrays.asList("ed9d1b37b0bd8b65ff9ce2688e0e102e")
         );
         executeTest("Testing SnpEff annotations", spec);
     }

From a91ac0c5dbb28667c38d69230827112af569ab55 Mon Sep 17 00:00:00 2001
From: Mark DePristo 
Date: Wed, 21 Sep 2011 10:15:05 -0400
Subject: [PATCH 32/41] Intermediate commit of bugfixes to CombineVariants

---
 .../org/broadinstitute/sting/utils/Utils.java |  40 ++++---
 .../variantcontext/VariantContextUtils.java   | 106 ++++++++----------
 .../CombineVariantsIntegrationTest.java       |  11 +-
 3 files changed, 82 insertions(+), 75 deletions(-)

diff --git a/public/java/src/org/broadinstitute/sting/utils/Utils.java b/public/java/src/org/broadinstitute/sting/utils/Utils.java
index f6edb319f..6ce492c63 100755
--- a/public/java/src/org/broadinstitute/sting/utils/Utils.java
+++ b/public/java/src/org/broadinstitute/sting/utils/Utils.java
@@ -240,22 +240,34 @@ public class Utils {
         return ret.toString();
     }
 
-    //public static String join(String separator, Collection strings) {
-    //    return join( separator, strings.toArray(new String[0]) );
-    //}
-
-    public static  String join(String separator, Collection objects) {
-        if(objects.isEmpty()) {
+    /**
+     * Returns a string of the form elt1.toString() [sep elt2.toString() ... sep elt.toString()] for a collection of
+     * elti objects (note there's no actual space between sep and the elti elements).  Returns
+     * "" if collection is empty.  If collection contains just elt, then returns elt.toString()
+     *
+     * @param separator the string to use to separate objects
+     * @param objects a collection of objects.  the element order is defined by the iterator over objects
+     * @param  the type of the objects
+     * @return a non-null string
+     */
+    public static  String join(final String separator, final Collection objects) {
+        if (objects.isEmpty()) { // fast path for empty collection
             return "";
-        }
-        Iterator iter = objects.iterator();
-        final StringBuilder ret = new StringBuilder(iter.next().toString());
-        while(iter.hasNext()) {
-            ret.append(separator);
-            ret.append(iter.next().toString());
-        }
+        } else {
+            final Iterator iter = objects.iterator();
+            final T first = iter.next();
 
-        return ret.toString();
+            if ( ! iter.hasNext() ) // fast path for singleton collections
+                return first.toString();
+            else { // full path for 2+ collection that actually need a join
+                final StringBuilder ret = new StringBuilder(first.toString());
+                while(iter.hasNext()) {
+                    ret.append(separator);
+                    ret.append(iter.next().toString());
+                }
+                return ret.toString();
+            }
+        }
     }
 
     public static String dupString(char c, int nCopies) {
diff --git a/public/java/src/org/broadinstitute/sting/utils/variantcontext/VariantContextUtils.java b/public/java/src/org/broadinstitute/sting/utils/variantcontext/VariantContextUtils.java
index 986d6305c..147538253 100755
--- a/public/java/src/org/broadinstitute/sting/utils/variantcontext/VariantContextUtils.java
+++ b/public/java/src/org/broadinstitute/sting/utils/variantcontext/VariantContextUtils.java
@@ -316,19 +316,22 @@ public class VariantContextUtils {
         return pruneVariantContext(vc, null);
     }
 
-    public static VariantContext pruneVariantContext(VariantContext vc, Collection keysToPreserve ) {
-        MutableVariantContext mvc = new MutableVariantContext(vc);
+    public static VariantContext pruneVariantContext(final VariantContext vc, final Collection keysToPreserve ) {
+        final MutableVariantContext mvc = new MutableVariantContext(vc);
 
         if ( keysToPreserve == null || keysToPreserve.size() == 0 )
             mvc.clearAttributes();
         else {
-            Map d = mvc.getAttributes();
+            final Map d = mvc.getAttributes();
             mvc.clearAttributes();
             for ( String key : keysToPreserve )
                 if ( d.containsKey(key) )
                     mvc.putAttribute(key, d.get(key));
         }
 
+        // this must be done as the ID is stored in the attributes field
+        if ( vc.hasID() ) mvc.setID(vc.getID());
+
         Collection gs = mvc.getGenotypes().values();
         mvc.clearGenotypes();
         for ( Genotype g : gs ) {
@@ -443,34 +446,6 @@ public class VariantContextUtils {
         throw new ReviewedStingException(String.format("Couldn't find master VCF %s at %s", masterName, unsortedVCs.iterator().next()));
     }
 
-
-    public static VariantContext simpleMerge(GenomeLocParser genomeLocParser, Collection unsortedVCs, byte refBase) {
-        return simpleMerge(genomeLocParser, unsortedVCs, null, FilteredRecordMergeType.KEEP_IF_ANY_UNFILTERED, GenotypeMergeType.UNSORTED, false, false, refBase);
-    }
-
-
-    /**
-     * Merges VariantContexts into a single hybrid.  Takes genotypes for common samples in priority order, if provided.
-     * If uniqifySamples is true, the priority order is ignored and names are created by concatenating the VC name with
-     * the sample name
-     *
-     * @param genomeLocParser           loc parser
-     * @param unsortedVCs               collection of unsorted VCs
-     * @param priorityListOfVCs         priority list detailing the order in which we should grab the VCs
-     * @param filteredRecordMergeType   merge type for filtered records
-     * @param genotypeMergeOptions      merge option for genotypes
-     * @param annotateOrigin            should we annotate the set it came from?
-     * @param printMessages             should we print messages?
-     * @param inputRefBase              the ref base
-     * @return new VariantContext
-     */
-    public static VariantContext simpleMerge(GenomeLocParser genomeLocParser, Collection unsortedVCs, List priorityListOfVCs,
-                                             FilteredRecordMergeType filteredRecordMergeType, GenotypeMergeType genotypeMergeOptions,
-                                             boolean annotateOrigin, boolean printMessages, byte inputRefBase ) {
-
-        return simpleMerge(genomeLocParser, unsortedVCs, priorityListOfVCs, filteredRecordMergeType, genotypeMergeOptions, annotateOrigin, printMessages, "set", false, false);
-    }
-
     /**
      * Merges VariantContexts into a single hybrid.  Takes genotypes for common samples in priority order, if provided.
      * If uniqifySamples is true, the priority order is ignored and names are created by concatenating the VC name with
@@ -486,12 +461,18 @@ public class VariantContextUtils {
      * @param setKey                    the key name of the set
      * @param filteredAreUncalled       are filtered records uncalled?
      * @param mergeInfoWithMaxAC        should we merge in info from the VC with maximum allele count?
-     * @return new VariantContext
+     * @return new VariantContext       representing the merge of unsortedVCs
      */
-    public static VariantContext simpleMerge(GenomeLocParser genomeLocParser, Collection unsortedVCs, List priorityListOfVCs,
-                                             FilteredRecordMergeType filteredRecordMergeType, GenotypeMergeType genotypeMergeOptions,
-                                             boolean annotateOrigin, boolean printMessages, String setKey,
-                                             boolean filteredAreUncalled, boolean mergeInfoWithMaxAC ) {
+    public static VariantContext simpleMerge(final GenomeLocParser genomeLocParser,
+                                             final Collection unsortedVCs,
+                                             final List priorityListOfVCs,
+                                             final FilteredRecordMergeType filteredRecordMergeType,
+                                             final GenotypeMergeType genotypeMergeOptions,
+                                             final boolean annotateOrigin,
+                                             final boolean printMessages,
+                                             final String setKey,
+                                             final boolean filteredAreUncalled,
+                                             final boolean mergeInfoWithMaxAC ) {
         if ( unsortedVCs == null || unsortedVCs.size() == 0 )
             return null;
 
@@ -514,26 +495,28 @@ public class VariantContextUtils {
             return null;
 
         // establish the baseline info from the first VC
-        VariantContext first = VCs.get(0);
-        String name = first.getSource();
-        GenomeLoc loc = getLocation(genomeLocParser,first);
+        final VariantContext first = VCs.get(0);
+        final String name = first.getSource();
+        final Allele refAllele = determineReferenceAllele(VCs);
 
-        Set alleles = new TreeSet();
-        Map genotypes = new TreeMap();
-        double negLog10PError = -1;
-        Set filters = new TreeSet();
-        Map attributes = new TreeMap();
-        Set inconsistentAttributes = new HashSet();
-        String rsID = null;
+        final Set alleles = new TreeSet();
+        final Set filters = new TreeSet();
+        final Map attributes = new TreeMap();
+        final Set inconsistentAttributes = new HashSet();
+        final Set variantSources = new HashSet(); // contains the set of sources we found in our set of VCs that are variant
+        final Set rsIDs = new LinkedHashSet(1); // most of the time there's one id
+
+        GenomeLoc loc = getLocation(genomeLocParser,first);
         int depth = 0;
         int maxAC = -1;
-        Map attributesWithMaxAC = new TreeMap();
+        final Map attributesWithMaxAC = new TreeMap();
+        double negLog10PError = -1;
         VariantContext vcWithMaxAC = null;
+        Map genotypes = new TreeMap();
 
         // counting the number of filtered and variant VCs
-        int nFiltered = 0, nVariant = 0;
+        int nFiltered = 0;
 
-        Allele refAllele = determineReferenceAllele(VCs);
         boolean remapped = false;
 
         // cycle through and add info from the other VCs, making sure the loc/reference matches
@@ -546,7 +529,7 @@ public class VariantContextUtils {
                 loc = getLocation(genomeLocParser,vc); // get the longest location
 
             nFiltered += vc.isFiltered() ? 1 : 0;
-            nVariant += vc.isVariant() ? 1 : 0;
+            if ( vc.isVariant() ) variantSources.add(vc.getSource());
 
             AlleleMapper alleleMapping = resolveIncompatibleAlleles(refAllele, vc, alleles);
             remapped = remapped || alleleMapping.needsRemapping();
@@ -566,8 +549,10 @@ public class VariantContextUtils {
             //
             if (vc.hasAttribute(VCFConstants.DEPTH_KEY))
                 depth += Integer.valueOf(vc.getAttributeAsString(VCFConstants.DEPTH_KEY));
-            if (rsID == null && vc.hasID())
-                rsID = vc.getID();
+
+            // TODO -- REVERT CHANGE
+            if (rsIDs.isEmpty() && vc.hasID()) rsIDs.add(vc.getID());
+
             if (mergeInfoWithMaxAC && vc.hasAttribute(VCFConstants.ALLELE_COUNT_KEY)) {
                 String rawAlleleCounts = vc.getAttributeAsString(VCFConstants.ALLELE_COUNT_KEY);
                 // lets see if the string contains a , separator
@@ -627,17 +612,16 @@ public class VariantContextUtils {
         if ( filteredRecordMergeType == FilteredRecordMergeType.KEEP_IF_ANY_UNFILTERED && nFiltered != VCs.size() )
             filters.clear();
 
-        // we care about where the call came from
-        if ( annotateOrigin ) {
+        if ( annotateOrigin ) { // we care about where the call came from
             String setValue;
-            if ( nFiltered == 0 && nVariant == priorityListOfVCs.size() )                   // nothing was unfiltered
+            if ( nFiltered == 0 && variantSources.size() == priorityListOfVCs.size() ) // nothing was unfiltered
                 setValue = "Intersection";
             else if ( nFiltered == VCs.size() )     // everything was filtered out
                 setValue = "FilteredInAll";
-            else if ( nVariant == 0 )               // everyone was reference
+            else if ( variantSources.isEmpty() )               // everyone was reference
                 setValue = "ReferenceInAll";
-            else {                                  // we are filtered in some subset
-                List s = new ArrayList();
+            else {
+                LinkedHashSet s = new LinkedHashSet();
                 for ( VariantContext vc : VCs )
                     if ( vc.isVariant() )
                         s.add( vc.isFiltered() ? "filterIn" + vc.getSource() : vc.getSource() );
@@ -652,8 +636,10 @@ public class VariantContextUtils {
 
         if ( depth > 0 )
             attributes.put(VCFConstants.DEPTH_KEY, String.valueOf(depth));
-        if ( rsID != null )
-            attributes.put(VariantContext.ID_KEY, rsID);
+
+        if ( ! rsIDs.isEmpty() ) {
+            attributes.put(VariantContext.ID_KEY, Utils.join(",", rsIDs));
+        }
 
         VariantContext merged = new VariantContext(name, loc.getContig(), loc.getStart(), loc.getStop(), alleles, genotypes, negLog10PError, filters, (mergeInfoWithMaxAC ? attributesWithMaxAC : attributes) );
         // Trim the padded bases of all alleles if necessary
diff --git a/public/java/test/org/broadinstitute/sting/gatk/walkers/variantutils/CombineVariantsIntegrationTest.java b/public/java/test/org/broadinstitute/sting/gatk/walkers/variantutils/CombineVariantsIntegrationTest.java
index 3267173a7..c6cf8454c 100755
--- a/public/java/test/org/broadinstitute/sting/gatk/walkers/variantutils/CombineVariantsIntegrationTest.java
+++ b/public/java/test/org/broadinstitute/sting/gatk/walkers/variantutils/CombineVariantsIntegrationTest.java
@@ -96,7 +96,7 @@ public class CombineVariantsIntegrationTest extends WalkerTest {
 
     @Test public void uniqueSNPs() { combine2("pilot2.snps.vcf4.genotypes.vcf", "yri.trio.gatk_glftrio.intersection.annotated.filtered.chr1.vcf", "", "89f55abea8f59e39d1effb908440548c"); }
 
-    @Test public void omniHM3Union() { combineSites(" -filteredRecordsMergeType KEEP_IF_ANY_UNFILTERED", "4836086891f6cbdd40eebef3076d215a"); }
+    @Test public void omniHM3Union() { combineSites(" -filteredRecordsMergeType KEEP_IF_ANY_UNFILTERED", "e6a053129c5f7b13129beefed9282155"); }
     @Test public void omniHM3Intersect() { combineSites(" -filteredRecordsMergeType KEEP_IF_ALL_UNFILTERED", "6a34b5d743efda8b2f3b639f3a2f5de8"); }
 
     @Test public void threeWayWithRefs() {
@@ -131,4 +131,13 @@ public class CombineVariantsIntegrationTest extends WalkerTest {
     @Test public void complexTestMinimal() { combineComplexSites(" -minimalVCF", "df96cb3beb2dbb5e02f80abec7d3571e"); }
     @Test public void complexTestSitesOnly() { combineComplexSites(" -sites_only", "f704caeaaaed6711943014b847fe381a"); }
     @Test public void complexTestSitesOnlyMinimal() { combineComplexSites(" -sites_only -minimalVCF", "f704caeaaaed6711943014b847fe381a"); }
+
+    @Test
+    public void combineDBSNPDuplicateSites() {
+         WalkerTestSpec spec = new WalkerTestSpec(
+                 "-T CombineVariants -NO_HEADER -L 1:902000-903000 -o %s -R " + b37KGReference + " -V:v1 " + b37dbSNP132,
+                 1,
+                 Arrays.asList(""));
+         executeTest("combineDBSNPDuplicateSites:", spec);
+    }
 }
\ No newline at end of file

From 34f435565ca294be93792e6998243116bf14f755 Mon Sep 17 00:00:00 2001
From: Mark DePristo 
Date: Wed, 21 Sep 2011 10:16:17 -0400
Subject: [PATCH 33/41] Accidentally committed unclean tribble jar to repo

---
 settings/repository/org.broad/tribble-25.jar | Bin 305986 -> 299110 bytes
 1 file changed, 0 insertions(+), 0 deletions(-)

diff --git a/settings/repository/org.broad/tribble-25.jar b/settings/repository/org.broad/tribble-25.jar
index 6d467ba3fdd6e6285e8244a87aefa4a0d232cfe1..e260764a5b7e4c676c5b260010ccad0dd46becfe 100644
GIT binary patch
delta 5549
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From 7d11f93b8270913e471ad92cc969c4c6257f9843 Mon Sep 17 00:00:00 2001
From: Mark DePristo 
Date: Wed, 21 Sep 2011 10:58:32 -0400
Subject: [PATCH 34/41] Final bugfix for CombineVariants

-- Now handles multiple records at a site, so that you don't see records like set=dbsnp-dbsnp-dbsnp when combining something with dbsnp
-- Proper handling of ids.  If you are merging files with multiple ids for the same record, the ids are merged into a comma separated list
---
 .../sting/utils/variantcontext/VariantContextUtils.java     | 3 +--
 .../variantutils/CombineVariantsIntegrationTest.java        | 6 +++---
 2 files changed, 4 insertions(+), 5 deletions(-)

diff --git a/public/java/src/org/broadinstitute/sting/utils/variantcontext/VariantContextUtils.java b/public/java/src/org/broadinstitute/sting/utils/variantcontext/VariantContextUtils.java
index 147538253..e0e27b4f7 100755
--- a/public/java/src/org/broadinstitute/sting/utils/variantcontext/VariantContextUtils.java
+++ b/public/java/src/org/broadinstitute/sting/utils/variantcontext/VariantContextUtils.java
@@ -550,8 +550,7 @@ public class VariantContextUtils {
             if (vc.hasAttribute(VCFConstants.DEPTH_KEY))
                 depth += Integer.valueOf(vc.getAttributeAsString(VCFConstants.DEPTH_KEY));
 
-            // TODO -- REVERT CHANGE
-            if (rsIDs.isEmpty() && vc.hasID()) rsIDs.add(vc.getID());
+            if ( vc.hasID() && ! vc.getID().equals(VCFConstants.EMPTY_ID_FIELD) ) rsIDs.add(vc.getID());
 
             if (mergeInfoWithMaxAC && vc.hasAttribute(VCFConstants.ALLELE_COUNT_KEY)) {
                 String rawAlleleCounts = vc.getAttributeAsString(VCFConstants.ALLELE_COUNT_KEY);
diff --git a/public/java/test/org/broadinstitute/sting/gatk/walkers/variantutils/CombineVariantsIntegrationTest.java b/public/java/test/org/broadinstitute/sting/gatk/walkers/variantutils/CombineVariantsIntegrationTest.java
index c6cf8454c..74ff850a4 100755
--- a/public/java/test/org/broadinstitute/sting/gatk/walkers/variantutils/CombineVariantsIntegrationTest.java
+++ b/public/java/test/org/broadinstitute/sting/gatk/walkers/variantutils/CombineVariantsIntegrationTest.java
@@ -90,14 +90,14 @@ public class CombineVariantsIntegrationTest extends WalkerTest {
 
     @Test public void combineTrioCalls() { combine2("CEU.trio.2010_03.genotypes.vcf.gz", "YRI.trio.2010_03.genotypes.vcf.gz", "", "1d5a021387a8a86554db45a29f66140f"); } // official project VCF files in tabix format
     @Test public void combineTrioCallsMin() { combine2("CEU.trio.2010_03.genotypes.vcf.gz", "YRI.trio.2010_03.genotypes.vcf.gz", " -minimalVCF", "20163d60f18a46496f6da744ab5cc0f9"); } // official project VCF files in tabix format
-    @Test public void combine2Indels() { combine2("CEU.dindel.vcf4.trio.2010_06.indel.genotypes.vcf", "CEU.dindel.vcf4.low_coverage.2010_06.indel.genotypes.vcf", "", "f1cf095c2fe9641b7ca1f8ee2c46fd4a"); }
+    @Test public void combine2Indels() { combine2("CEU.dindel.vcf4.trio.2010_06.indel.genotypes.vcf", "CEU.dindel.vcf4.low_coverage.2010_06.indel.genotypes.vcf", "", "d76cd5b3ced7745d42fe0af39ce0b32e"); }
 
     @Test public void combineSNPsAndIndels() { combine2("CEU.trio.2010_03.genotypes.vcf.gz", "CEU.dindel.vcf4.low_coverage.2010_06.indel.genotypes.vcf", "", "e144b6283765494bfe8189ac59965083"); }
 
     @Test public void uniqueSNPs() { combine2("pilot2.snps.vcf4.genotypes.vcf", "yri.trio.gatk_glftrio.intersection.annotated.filtered.chr1.vcf", "", "89f55abea8f59e39d1effb908440548c"); }
 
-    @Test public void omniHM3Union() { combineSites(" -filteredRecordsMergeType KEEP_IF_ANY_UNFILTERED", "e6a053129c5f7b13129beefed9282155"); }
-    @Test public void omniHM3Intersect() { combineSites(" -filteredRecordsMergeType KEEP_IF_ALL_UNFILTERED", "6a34b5d743efda8b2f3b639f3a2f5de8"); }
+    @Test public void omniHM3Union() { combineSites(" -filteredRecordsMergeType KEEP_IF_ANY_UNFILTERED", "c6adeda751cb2a08690dd9202356629f"); }
+    @Test public void omniHM3Intersect() { combineSites(" -filteredRecordsMergeType KEEP_IF_ALL_UNFILTERED", "3a08fd5ee18993dfc8882156ccf5d2e9"); }
 
     @Test public void threeWayWithRefs() {
         WalkerTestSpec spec = new WalkerTestSpec(

From ecc7f34774df37969a7d333d298bc803963c6159 Mon Sep 17 00:00:00 2001
From: Mark DePristo 
Date: Wed, 21 Sep 2011 11:09:54 -0400
Subject: [PATCH 35/41] Putative fix for BAQ problem.

---
 .../java/src/org/broadinstitute/sting/utils/baq/BAQ.java  | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/public/java/src/org/broadinstitute/sting/utils/baq/BAQ.java b/public/java/src/org/broadinstitute/sting/utils/baq/BAQ.java
index ef7cf751e..1723557c4 100644
--- a/public/java/src/org/broadinstitute/sting/utils/baq/BAQ.java
+++ b/public/java/src/org/broadinstitute/sting/utils/baq/BAQ.java
@@ -5,6 +5,7 @@ import net.sf.picard.reference.ReferenceSequence;
 import net.sf.samtools.CigarElement;
 import net.sf.samtools.CigarOperator;
 import net.sf.samtools.SAMRecord;
+import net.sf.samtools.SAMUtils;
 import org.broadinstitute.sting.utils.collections.Pair;
 import org.broadinstitute.sting.utils.exceptions.ReviewedStingException;
 import org.broadinstitute.sting.utils.exceptions.UserException;
@@ -131,19 +132,18 @@ public class BAQ {
     private final static double EM = 0.33333333333;
     private final static double EI = 0.25;
 
-    private double[][][] EPSILONS = new double[256][256][64];
+    private double[][][] EPSILONS = new double[256][256][SAMUtils.MAX_PHRED_SCORE+1];
 
     private void initializeCachedData() {
         for ( int i = 0; i < 256; i++ )
             for ( int j = 0; j < 256; j++ )
-                for ( int q = 0; q < 64; q++ ) {
-                    double qual = qual2prob[q < minBaseQual ? minBaseQual : q];
+                for ( int q = 0; q <= SAMUtils.MAX_PHRED_SCORE; q++ ) {
                     EPSILONS[i][j][q] = 1.0;
                 }
 
         for ( char b1 : "ACGTacgt".toCharArray() ) {
             for ( char b2 : "ACGTacgt".toCharArray() ) {
-                for ( int q = 0; q < 64; q++ ) {
+                for ( int q = 0; q <= SAMUtils.MAX_PHRED_SCORE; q++ ) {
                     double qual = qual2prob[q < minBaseQual ? minBaseQual : q];
                     double e = Character.toLowerCase(b1) == Character.toLowerCase(b2) ? 1 - qual : qual * EM;
                     EPSILONS[(byte)b1][(byte)b2][q] = e;

From 174859fc68c434ee568884ea9d9e53541070b24a Mon Sep 17 00:00:00 2001
From: Eric Banks 
Date: Wed, 21 Sep 2011 11:14:54 -0400
Subject: [PATCH 36/41] Don't allow whitespace in the INFO field

---
 .../sting/utils/codecs/vcf/AbstractVCFCodec.java             | 5 ++++-
 1 file changed, 4 insertions(+), 1 deletion(-)

diff --git a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/AbstractVCFCodec.java b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/AbstractVCFCodec.java
index 624d06a71..18646b057 100755
--- a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/AbstractVCFCodec.java
+++ b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/AbstractVCFCodec.java
@@ -215,7 +215,7 @@ public abstract class AbstractVCFCodec implements FeatureCodec, NameAwareCodec,
             int nParts = ParsingUtils.split(line, parts, VCFConstants.FIELD_SEPARATOR_CHAR, true);
 
             // if we have don't have a header, or we have a header with no genotyping data check that we have eight columns.  Otherwise check that we have nine (normal colummns + genotyping data)
-            if (( (header == null || (header != null && !header.hasGenotypingData())) && nParts != NUM_STANDARD_FIELDS) ||
+            if (( (header == null || !header.hasGenotypingData()) && nParts != NUM_STANDARD_FIELDS) ||
                  (header != null && header.hasGenotypingData() && nParts != (NUM_STANDARD_FIELDS + 1)) )
                 throw new UserException.MalformedVCF("there aren't enough columns for line " + line + " (we expected " + (header == null ? NUM_STANDARD_FIELDS : NUM_STANDARD_FIELDS + 1) +
                         " tokens, and saw " + nParts + " )", lineNo);
@@ -345,6 +345,9 @@ public abstract class AbstractVCFCodec implements FeatureCodec, NameAwareCodec,
             generateException("The VCF specification requires a valid info field");
 
         if ( !infoField.equals(VCFConstants.EMPTY_INFO_FIELD) ) {
+            if ( infoField.indexOf("\t") != -1 || infoField.indexOf(" ") != -1 )
+                generateException("The VCF specification does not allow for whitespace in the INFO field");
+
             int infoValueSplitSize = ParsingUtils.split(infoField, infoValueArray, VCFConstants.INFO_FIELD_SEPARATOR_CHAR);
             for (int i = 0; i < infoValueSplitSize; i++) {
                 String key;

From 6592972f82bab1bc0903460241b7b1656902e4cd Mon Sep 17 00:00:00 2001
From: Mark DePristo 
Date: Wed, 21 Sep 2011 11:25:08 -0400
Subject: [PATCH 37/41] Putative fix for BAQ array out of bounds

-- Old code required qual to be <64, which isn't strictly necessary.  Now uses the Picard SAMUtils.MAX_PHRED_SCORE constant
-- Unittest to enforce this behavior
---
 .../src/org/broadinstitute/sting/utils/baq/BAQ.java   |  2 +-
 .../broadinstitute/sting/utils/baq/BAQUnitTest.java   | 11 +++++++++++
 2 files changed, 12 insertions(+), 1 deletion(-)

diff --git a/public/java/src/org/broadinstitute/sting/utils/baq/BAQ.java b/public/java/src/org/broadinstitute/sting/utils/baq/BAQ.java
index 1723557c4..4f096f86e 100644
--- a/public/java/src/org/broadinstitute/sting/utils/baq/BAQ.java
+++ b/public/java/src/org/broadinstitute/sting/utils/baq/BAQ.java
@@ -152,7 +152,7 @@ public class BAQ {
         }
     }
 
-    private double calcEpsilon( byte ref, byte read, byte qualB ) {
+    protected double calcEpsilon( byte ref, byte read, byte qualB ) {
         return EPSILONS[ref][read][qualB];
     }
 
diff --git a/public/java/test/org/broadinstitute/sting/utils/baq/BAQUnitTest.java b/public/java/test/org/broadinstitute/sting/utils/baq/BAQUnitTest.java
index 2e4dac6da..67943ccb4 100644
--- a/public/java/test/org/broadinstitute/sting/utils/baq/BAQUnitTest.java
+++ b/public/java/test/org/broadinstitute/sting/utils/baq/BAQUnitTest.java
@@ -172,6 +172,17 @@ public class BAQUnitTest extends BaseTest {
         }
     }
 
+    @Test(enabled = true)
+    public void testBAQQualRange() {
+        BAQ baq = new BAQ(1e-3, 0.1, 7, (byte)4, false);         // matches current samtools parameters
+        final byte ref = (byte)'A';
+        final byte alt = (byte)'A';
+
+        for ( int i = 0; i <= SAMUtils.MAX_PHRED_SCORE; i++ )
+            Assert.assertTrue(baq.calcEpsilon( ref, alt, (byte)i) >= 0.0, "Failed to get baq epsilon range");
+    }
+
+
     public void testBAQ(BAQTest test, boolean lookupWithFasta) {
         BAQ baqHMM = new BAQ(1e-3, 0.1, 7, (byte)4, false);         // matches current samtools parameters
 

From 2585fc3d6cc4dd873eed2868fef8335d7e40a504 Mon Sep 17 00:00:00 2001
From: Ryan Poplin 
Date: Wed, 21 Sep 2011 15:22:26 -0400
Subject: [PATCH 38/41] Updating Rscript path doc text for Broad users

---
 .../sting/analyzecovariates/AnalyzeCovariates.java              | 2 +-
 .../gatk/walkers/variantrecalibration/VariantRecalibrator.java  | 2 +-
 .../src/org/broadinstitute/sting/utils/R/RScriptExecutor.java   | 2 +-
 3 files changed, 3 insertions(+), 3 deletions(-)

diff --git a/public/java/src/org/broadinstitute/sting/analyzecovariates/AnalyzeCovariates.java b/public/java/src/org/broadinstitute/sting/analyzecovariates/AnalyzeCovariates.java
index 7ea515591..1ef452a5c 100755
--- a/public/java/src/org/broadinstitute/sting/analyzecovariates/AnalyzeCovariates.java
+++ b/public/java/src/org/broadinstitute/sting/analyzecovariates/AnalyzeCovariates.java
@@ -114,7 +114,7 @@ public class AnalyzeCovariates extends CommandLineProgram {
     private String RECAL_FILE = "output.recal_data.csv";
     @Argument(fullName = "output_dir", shortName = "outputDir", doc = "The directory in which to output all the plots and intermediate data files", required = false)
     private String OUTPUT_DIR = "analyzeCovariates/";
-    @Argument(fullName = "path_to_Rscript", shortName = "Rscript", doc = "The path to your implementation of Rscript. For Broad users this is maybe /broad/tools/apps/R-2.6.0/bin/Rscript", required = false)
+    @Argument(fullName = "path_to_Rscript", shortName = "Rscript", doc = "The path to your implementation of Rscript. For Broad users this is maybe /broad/software/free/Linux/redhat_5_x86_64/pkgs/r_2.12.0/bin/Rscript", required = false)
     private String PATH_TO_RSCRIPT = "Rscript";
     @Argument(fullName = "path_to_resources", shortName = "resources", doc = "Path to resources folder holding the Sting R scripts.", required = false)
     private String PATH_TO_RESOURCES = "public/R/";
diff --git a/public/java/src/org/broadinstitute/sting/gatk/walkers/variantrecalibration/VariantRecalibrator.java b/public/java/src/org/broadinstitute/sting/gatk/walkers/variantrecalibration/VariantRecalibrator.java
index 529d17285..89e702b64 100755
--- a/public/java/src/org/broadinstitute/sting/gatk/walkers/variantrecalibration/VariantRecalibrator.java
+++ b/public/java/src/org/broadinstitute/sting/gatk/walkers/variantrecalibration/VariantRecalibrator.java
@@ -155,7 +155,7 @@ public class VariantRecalibrator extends RodWalker
Date: Wed, 21 Sep 2011 15:25:01 -0400
Subject: [PATCH 39/41] Marginally cleaner isVCFStream() function

-- cleanup trying to debug minor bug.  Failed to fix the bug, but the code is nicer now
---
 .../sting/utils/codecs/vcf/AbstractVCFCodec.java      | 11 +++++++----
 1 file changed, 7 insertions(+), 4 deletions(-)

diff --git a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/AbstractVCFCodec.java b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/AbstractVCFCodec.java
index 18646b057..e8d1dc6d6 100755
--- a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/AbstractVCFCodec.java
+++ b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/AbstractVCFCodec.java
@@ -601,12 +601,15 @@ public abstract class AbstractVCFCodec implements FeatureCodec, NameAwareCodec,
     private final static boolean isVCFStream(final InputStream stream, final String MAGIC_HEADER_LINE) {
         try {
             byte[] buff = new byte[MAGIC_HEADER_LINE.length()];
-            stream.read(buff, 0, MAGIC_HEADER_LINE.length());
-            String firstLine = new String(buff);
-            stream.close();
-            return firstLine.startsWith(MAGIC_HEADER_LINE);
+            int nread = stream.read(buff, 0, MAGIC_HEADER_LINE.length());
+            boolean eq = Arrays.equals(buff, MAGIC_HEADER_LINE.getBytes());
+            return eq;
+//            String firstLine = new String(buff);
+//            return firstLine.startsWith(MAGIC_HEADER_LINE);
         } catch ( IOException e ) {
             return false;
+        } finally {
+            try { stream.close(); } catch ( IOException e ) {}
         }
     }
 }

From 6bcfce225f42e31a03d721b119410da7c9063f5d Mon Sep 17 00:00:00 2001
From: Mark DePristo 
Date: Wed, 21 Sep 2011 15:39:19 -0400
Subject: [PATCH 40/41] Fix for dynamic type determination for bgzip files

-- GZipInputStream handles bgzip files under linux, but not mac
-- Added BlockCompressedInputStream test as well, which works properly on bgzip files
---
 .../sting/utils/codecs/vcf/AbstractVCFCodec.java            | 6 +++++-
 .../sting/gatk/refdata/tracks/FeatureManagerUnitTest.java   | 2 ++
 2 files changed, 7 insertions(+), 1 deletion(-)

diff --git a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/AbstractVCFCodec.java b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/AbstractVCFCodec.java
index e8d1dc6d6..83c7083d0 100755
--- a/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/AbstractVCFCodec.java
+++ b/public/java/src/org/broadinstitute/sting/utils/codecs/vcf/AbstractVCFCodec.java
@@ -6,6 +6,7 @@ import org.broad.tribble.FeatureCodec;
 import org.broad.tribble.NameAwareCodec;
 import org.broad.tribble.TribbleException;
 import org.broad.tribble.readers.LineReader;
+import org.broad.tribble.util.BlockCompressedInputStream;
 import org.broad.tribble.util.ParsingUtils;
 import org.broadinstitute.sting.gatk.refdata.SelfScopingFeatureCodec;
 import org.broadinstitute.sting.utils.exceptions.ReviewedStingException;
@@ -590,7 +591,8 @@ public abstract class AbstractVCFCodec implements FeatureCodec, NameAwareCodec,
     public final static boolean canDecodeFile(final File potentialInput, final String MAGIC_HEADER_LINE) {
         try {
             return isVCFStream(new FileInputStream(potentialInput), MAGIC_HEADER_LINE) ||
-                    isVCFStream(new GZIPInputStream(new FileInputStream(potentialInput)), MAGIC_HEADER_LINE);
+                    isVCFStream(new GZIPInputStream(new FileInputStream(potentialInput)), MAGIC_HEADER_LINE) ||
+                    isVCFStream(new BlockCompressedInputStream(new FileInputStream(potentialInput)), MAGIC_HEADER_LINE);
         } catch ( FileNotFoundException e ) {
             return false;
         } catch ( IOException e ) {
@@ -608,6 +610,8 @@ public abstract class AbstractVCFCodec implements FeatureCodec, NameAwareCodec,
 //            return firstLine.startsWith(MAGIC_HEADER_LINE);
         } catch ( IOException e ) {
             return false;
+        } catch ( RuntimeException e ) {
+            return false;
         } finally {
             try { stream.close(); } catch ( IOException e ) {}
         }
diff --git a/public/java/test/org/broadinstitute/sting/gatk/refdata/tracks/FeatureManagerUnitTest.java b/public/java/test/org/broadinstitute/sting/gatk/refdata/tracks/FeatureManagerUnitTest.java
index bae8e99ed..e8799e2ab 100644
--- a/public/java/test/org/broadinstitute/sting/gatk/refdata/tracks/FeatureManagerUnitTest.java
+++ b/public/java/test/org/broadinstitute/sting/gatk/refdata/tracks/FeatureManagerUnitTest.java
@@ -56,6 +56,7 @@ public class FeatureManagerUnitTest extends BaseTest {
     private static final File VCF3_FILE = new File(validationDataLocation + "vcfexample3.vcf");
     private static final File VCF4_FILE = new File(testDir + "HiSeq.10000.vcf");
     private static final File VCF4_FILE_GZ = new File(testDir + "HiSeq.10000.vcf.gz");
+    private static final File VCF4_FILE_BGZIP = new File(testDir + "HiSeq.10000.bgzip.vcf.gz");
 
     private FeatureManager manager;
     private GenomeLocParser genomeLocParser;
@@ -109,6 +110,7 @@ public class FeatureManagerUnitTest extends BaseTest {
         new FMTest(VariantContext.class, VCF3Codec.class, "VCF3", VCF3_FILE);
         new FMTest(VariantContext.class, VCFCodec.class, "VCF", VCF4_FILE);
         new FMTest(VariantContext.class, VCFCodec.class, "VCF", VCF4_FILE_GZ);
+        new FMTest(VariantContext.class, VCFCodec.class, "VCF", VCF4_FILE_BGZIP);
         new FMTest(TableFeature.class, BedTableCodec.class, "bedtable", null);
         return FMTest.getTests(FMTest.class);
     }

From c6ba94471985e3956b2108063c9f65d350d170cb Mon Sep 17 00:00:00 2001
From: Mark DePristo 
Date: Wed, 21 Sep 2011 15:39:45 -0400
Subject: [PATCH 41/41] Adding bgzip vcf file for unit tests

---
 public/testdata/HiSeq.10000.bgzip.vcf.gz | Bin 0 -> 509234 bytes
 1 file changed, 0 insertions(+), 0 deletions(-)
 create mode 100644 public/testdata/HiSeq.10000.bgzip.vcf.gz

diff --git a/public/testdata/HiSeq.10000.bgzip.vcf.gz b/public/testdata/HiSeq.10000.bgzip.vcf.gz
new file mode 100644
index 0000000000000000000000000000000000000000..3f2b9bf14a3e046fe2b7dd71ffdbf23e6c521bd9
GIT binary patch
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