More accessors. Can compute the sum of the quality scores in the read (useful for sorting) and can return a subset of itself.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@948 348d0f76-0448-11de-a6fe-93d51630548a
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@ -5,7 +5,7 @@ package org.broadinstitute.sting.secondarybase;
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*
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* @author Kiran Garimella
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*/
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public class RawRead {
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public class RawRead implements Comparable<RawRead> {
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private byte lane;
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private short tile;
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private short x;
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@ -15,6 +15,21 @@ public class RawRead {
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private byte[] quals;
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private short[][] intensities;
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/**
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* Blank constructor.
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*/
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public RawRead() {}
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/**
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* Construct a raw read from the output of a PasteParser (in the order of int, seq, prb).
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* Takes data from entire read.
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*
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* @param pastedReadString the 3x(fragment length) output array from the PasteParser.
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*/
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public RawRead(String[][] pastedReadString) {
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loadRange(pastedReadString, 0, pastedReadString[1][4].length() - 1);
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}
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/**
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* Construct a raw read from the output of a PasteParser (in the order of int, seq, prb).
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* Takes data within specified cycle ranges.
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@ -24,6 +39,17 @@ public class RawRead {
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* @param cycleEnd the end cycle for the read (0-based, inclusive)
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*/
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public RawRead(String[][] pastedReadString, int cycleBegin, int cycleEnd) {
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loadRange(pastedReadString, cycleBegin, cycleEnd);
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}
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/**
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* Does the actual parsing of the PasteParser output.
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*
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* @param pastedReadString the 3x(fragment length) output array from the PasteParser.
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* @param cycleBegin the start cycle for the read (0-based, inclusive)
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* @param cycleEnd the end cycle for the read (0-based, inclusive)
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*/
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private void loadRange(String pastedReadString[][], int cycleBegin, int cycleEnd) {
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lane = Byte.valueOf(pastedReadString[0][0]);
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tile = Short.valueOf(pastedReadString[0][1]);
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x = Short.valueOf(pastedReadString[0][2]);
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@ -61,12 +87,26 @@ public class RawRead {
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*/
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public byte getLane() { return lane; }
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/**
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* Set lane number of read.
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*
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* @param lane lane number of read
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*/
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public void setLane(byte lane) { this.lane = lane; }
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/**
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* Get tile number of read.
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*
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* @return tile number of read
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*/
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public int getTile() { return tile; }
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public short getTile() { return tile; }
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/**
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* Set tile number of read.
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*
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* @param tile tile number of read
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*/
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public void setTile(short tile) { this.tile = tile; }
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/**
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* Get x-coordinate of read.
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@ -75,6 +115,13 @@ public class RawRead {
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*/
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public int getXCoordinate() { return x; }
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/**
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* Set x-coordinate of read.
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*
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* @param x x-coordinate of read
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*/
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public void setXCoordinate(short x) { this.x = x; }
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/**
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* Get y-coordinate of read.
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*
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@ -82,6 +129,13 @@ public class RawRead {
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*/
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public int getYCoordinate() { return y; }
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/**
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* Set y-coordinate of read.
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*
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* @param y y-coordinate of read
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*/
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public void setYCoordinate(short y) { this.y = y; }
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/**
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* Get read key (lane:tile:x:y).
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*
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@ -108,9 +162,7 @@ public class RawRead {
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*
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* @return the read sequence in String form
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*/
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public String getSequenceAsString() {
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return new String(getSequence());
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}
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public String getSequenceAsString() { return new String(getSequence()); }
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/**
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* Get the quals.
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@ -146,4 +198,59 @@ public class RawRead {
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* @return the read length
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*/
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public int getReadLength() { return sequence.length; }
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/**
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* Return the sum of the quality scores for this RawRead.
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*
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* @return the sum of the quality scores
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*/
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public int getQualityScoreSum() {
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int qualSum = 0;
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for ( byte qual : quals ) {
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qualSum += (int) qual;
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}
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return qualSum;
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}
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/**
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* Compare two RawRead objects by summing their quality scores. The one with lower aggregate quality is the "lesser" RawRead.
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*
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* @param rawRead the other RawRead
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* @return -1, 0, or 1 if the RawRead on which this method is called is the lesser one, is equal to the comparison RawRead, or is greater than the comparison RawRead, respectively.
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*/
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public int compareTo(RawRead rawRead) {
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int qualSum1 = this.getQualityScoreSum();
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int qualSum2 = rawRead.getQualityScoreSum();
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if (qualSum1 < qualSum2) { return -1; }
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else if (qualSum1 > qualSum2) { return 1; }
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return 0;
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}
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public RawRead getSubset(int cycleStart, int cycleStop) {
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RawRead subRead = new RawRead();
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subRead.setLane(lane);
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subRead.setTile(tile);
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subRead.setXCoordinate(x);
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subRead.setYCoordinate(y);
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byte[] newSequence = new byte[cycleStop - cycleStart + 1];
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byte[] newQuals = new byte[cycleStop - cycleStart + 1];
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short[][] newIntensities = new short[cycleStop - cycleStart + 1][4];
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for (int cycle = cycleStart, offset = 0; cycle <= cycleStop; cycle++, offset++) {
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newSequence[offset] = sequence[cycle];
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newQuals[offset] = quals[cycle];
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newIntensities[offset] = intensities[cycle];
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}
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subRead.setSequence(newSequence);
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subRead.setQuals(newQuals);
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subRead.setIntensities(newIntensities);
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return subRead;
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}
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}
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