Fixing up some comments in the BQSR
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@ -100,7 +100,7 @@ public class CountCovariatesWalker extends LocusWalker<CountCovariatesWalker.Cou
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/////////////////////////////
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// Command Line Arguments
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/////////////////////////////
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@Output(fullName="recal_file", shortName="recalFile", required=true, doc="Filename for the outputted covariates table recalibration file")
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@Output(fullName="recal_file", shortName="recalFile", required=true, doc="Filename for the output covariates table recalibration file")
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@Gather(CountCovariatesGatherer.class)
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public PrintStream RECAL_FILE;
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@ -124,8 +124,8 @@ public class CountCovariatesWalker extends LocusWalker<CountCovariatesWalker.Cou
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/////////////////////////////
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private final RecalDataManager dataManager = new RecalDataManager(); // Holds the data HashMap, mostly used by TableRecalibrationWalker to create collapsed data hashmaps
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private final ArrayList<Covariate> requestedCovariates = new ArrayList<Covariate>(); // A list to hold the covariate objects that were requested
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private static final double DBSNP_VS_NOVEL_MISMATCH_RATE = 2.0; // rate at which dbSNP sites (on an individual level) mismatch relative to novel sites (determined by looking at NA12878)
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private static int DBSNP_VALIDATION_CHECK_FREQUENCY = 1000000; // how often to validate dbsnp mismatch rate (in terms of loci seen)
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private static final double DBSNP_VS_NOVEL_MISMATCH_RATE = 2.0; // rate at which dbSNP sites (on an individual level) mismatch relative to novel sites (determined by looking at NA12878)
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private static int DBSNP_VALIDATION_CHECK_FREQUENCY = 1000000; // how often to validate dbsnp mismatch rate (in terms of loci seen)
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public static class CountedData {
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private long countedSites = 0; // Number of loci used in the calculations, used for reporting in the output file
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@ -136,7 +136,7 @@ public class CountCovariatesWalker extends LocusWalker<CountCovariatesWalker.Cou
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private long dbSNPCountsMM = 0, dbSNPCountsBases = 0; // mismatch/base counts for dbSNP loci
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private long novelCountsMM = 0, novelCountsBases = 0; // mismatch/base counts for non-dbSNP loci
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private int lociSinceLastDbsnpCheck = 0; // loci since last dbsnp validation
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private int lociSinceLastDbsnpCheck = 0; // loci since last dbsnp validation
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/**
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* Adds the values of other to this, returning this
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@ -298,8 +298,8 @@ public class CountCovariatesWalker extends LocusWalker<CountCovariatesWalker.Cou
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CountedData counter = new CountedData();
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if( !isSNP ) {
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// For each read at this locus
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for( PileupElement p : context.getBasePileup() ) {
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GATKSAMRecord gatkRead = (GATKSAMRecord) p.getRead();
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for( final PileupElement p : context.getBasePileup() ) {
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final GATKSAMRecord gatkRead = (GATKSAMRecord) p.getRead();
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int offset = p.getOffset();
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if( gatkRead.containsTemporaryAttribute( SKIP_RECORD_ATTRIBUTE ) ) {
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@ -93,8 +93,7 @@ public class TableRecalibrationWalker extends ReadWalker<SAMRecord, SAMFileWrite
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@Output(doc="The output BAM file", required=true)
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private StingSAMFileWriter OUTPUT_BAM = null;
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@Argument(fullName="preserve_qscores_less_than", shortName="pQ",
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doc="Bases with quality scores less than this threshold won't be recalibrated, default=5. In general it's unsafe to change qualities scores below < 5, since base callers use these values to indicate random or bad bases", required=false)
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@Argument(fullName="preserve_qscores_less_than", shortName="pQ", doc="Bases with quality scores less than this threshold won't be recalibrated, default=5. In general it's unsafe to change qualities scores below < 5, since base callers use these values to indicate random or bad bases", required=false)
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private int PRESERVE_QSCORES_LESS_THAN = 5;
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@Argument(fullName="smoothing", shortName="sm", required = false, doc="Number of imaginary counts to add to each bin in order to smooth out bins with few data points, default=1")
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private int SMOOTHING = 1;
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