more powerful management routines for my pipeline

git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@3351 348d0f76-0448-11de-a6fe-93d51630548a

python/farm_commands2.py

@@ -109,7 +109,7 @@ def executeJob(job, farm_queue = None, just_print_commands = False, debug = True
         job.jobID = justPrintJobIDCounter
         justPrintJobIDCounter += 1
     elif farm_queue:
-        print 'job.executionString', job.executionString
+        #print 'job.executionString', job.executionString
         result = subprocess.Popen([job.executionString, ""], shell=True, stdout=subprocess.PIPE).communicate()[0]
         p = re.compile('Job <(\d+)> is submitted to queue')
         job.jobID = p.match(result).group(1)

python/madPipelineUtils.py

@@ -17,11 +17,11 @@ GATK_JAR = GATK_STABLE_JAR
 # add to GATK to enable dbSNP aware cleaning
 # -D /humgen/gsa-scr1/GATK_Data/dbsnp_129_hg18.rod
 
-#hg18 = ['chrM'] + ['chr' + str(i) for i in range(1,23)] + ['chrX', 'chrY']
-#b36 = [str(i) for i in range(1,23)] + ['X', 'Y', 'MT']
+hg18 = ['chrM'] + ['chr' + str(i) for i in range(1,23)] + ['chrX', 'chrY']
+b36 = [str(i) for i in range(1,23)] + ['X', 'Y', 'MT']
 
-hg18 = ['chr' + str(i) for i in range(1,23)] + ['chrX', 'chrY']
-b36 = [str(i) for i in range(1,23)] + ['X', 'Y']
+#hg18 = ['chr' + str(i) for i in range(1,23)] + ['chrX', 'chrY']
+#b36 = [str(i) for i in range(1,23)] + ['X', 'Y']
 
 HG18_TO_B36 = {
     'hg18' : 'b36',
@@ -45,11 +45,12 @@ def appendExtension(path, newExt, addExtension = True):
 #    return os.path.join(OPTIONS.dir, s)
 
 class PipelineArgs:
-    def __init__( self, GATK_JAR = GATK_JAR, ref = 'hg18', name = None, memory = '4g' ):
+    def __init__( self, GATK_JAR = GATK_JAR, ref = 'hg18', name = None, memory = '4g', excludeChrs = [] ):
         self.GATK = 'java -Xmx%s -Djava.io.tmpdir=/broad/shptmp/depristo/tmp/ -jar ' + GATK_JAR + ' -R /seq/references/Homo_sapiens_assembly18/v0/Homo_sapiens_assembly18.fasta -l INFO '
         self.ref = ref
         self.name = name
         self.memory = memory
+        self.excludeChrs = excludeChrs
 
     def convertToB36(self): 
         return self.ref == 'b36'
@@ -71,6 +72,9 @@ class PipelineArgs:
             cmd = hg18args_to_b36(cmd)
         return cmd
         
+    def chrsToSplitBy(self, chrs):
+        return filter(lambda x: x not in self.excludeChrs, chrs)
+        
 # 
 # General features
 #
@@ -97,7 +101,7 @@ def splitGATKCommandByChr( myPipelineArgs, cmd, outputsToParallelize, mergeComma
         chrCmd = FarmJob(chr_cmd_str, jobName = cmd.jobName + '.byChr' + chr, dependencies = cmd.dependencies)
         return chrCmd, chrOutputMap
 
-    splits = map( makeChrCmd, hg18 )
+    splits = map( makeChrCmd, myPipelineArgs.chrsToSplitBy(hg18) )
     splitCommands = map(lambda x: x[0], splits)
 
     def mergeCommand1(i):

python/realignBamByChr.py

@@ -9,6 +9,7 @@ import faiReader
 import math
 import shutil
 import string
+import picard_utils
 from madPipelineUtils import *
 
 def main():
@@ -42,7 +43,7 @@ def main():
     inputBam, outputRoot = args[1:]
     outputBamList = outputRoot + '.bams.list'
     
-    STAGES = ['targets', 'realign', 'index']
+    STAGES = ['targets', 'realign', 'index', 'merge']
     for stage in stages:
         if stage not in STAGES:
             sys.exit('unknown stage ' + stage)
@@ -57,6 +58,7 @@ def main():
         return name in stages
 
     out = open(outputBamList, 'w')
+    realignInfo = []
     for chr in hg18:
         lastJobs = None
         
@@ -66,23 +68,30 @@ def main():
             allJobs.append(newjobs)
             if newjobs != []: 
                 lastJobs = newjobs
-            return [], lastJobs
-
-        newJobs = []
+            return lastJobs
 
         def execStage(name, func, args = [], lastJobs = []):
             if OPTIONS.verbose: print 'Name is', name
             newJobs, results = func(myPipelineArgs, chr, inputBam, outputRoot + '.' + chr, args, lastJobs)
-            if includeStage(name): newJobs, lastJobs = updateNewJobs(newJobs, lastJobs)
-            return newJobs, lastJobs, results
+            if includeStage(name): lastJobs = updateNewJobs(newJobs, lastJobs)
+            return lastJobs, results
             
-        newJobs, lastJobs, intervals = execStage('targets', createTargets)
-        newJobs, lastJobs, realignedBam = execStage('realign', realign, intervals, lastJobs)
+        lastJobs, intervals = execStage('targets', createTargets)
+        realignJobs, realignedBam = execStage('realign', realign, intervals, lastJobs)
+        realignInfo.append([realignJobs, realignedBam])
         # need to merge and then index
-        newJobs, lastJobs, ignore = execStage('index', index, realignedBam, lastJobs)
+        indexJobs, ignore = execStage('index', index, realignedBam, realignJobs)
         print >> out, os.path.abspath(realignedBam)
      
     out.close() 
+
+    if 'merge' in stages:
+        realignerJobs = []
+        if realignInfo[0][0] != []:
+            realignerJobs = map(lambda x: x[0][0], realignInfo)
+        mergerJob = mergeBams(myPipelineArgs, outputRoot + ".bam", map(lambda x: x[1], realignInfo), realignerJobs)
+        allJobs.append(mergerJob)
+
     print 'EXECUTING JOBS'
     executeJobs(allJobs, farm_queue = OPTIONS.farmQueue, just_print_commands = OPTIONS.dry) 
 
@@ -99,5 +108,10 @@ def realign( myPipelineArgs, chr, inputBam, outputRoot, intervals, lastJobs ):
 def index( myPipelineArgs, chr, inputBam, outputRoot, realignedBam, lastJobs ):
     return indexBAMFile( myPipelineArgs.name, realignedBam, lastJobs )
 
+def mergeBams( myPipelineArgs, outputFilename, bamsToMerge, lastJobs ):
+    print lastJobs
+    cmd = picard_utils.mergeBAMCmd( outputFilename, bamsToMerge, compression_level = 5 )
+    return FarmJob(cmd, jobName = 'merge.' + myPipelineArgs.name, dependencies = lastJobs)
+
 if __name__ == "__main__":
     main()