Moved platform flow information into NGSPlatform
* Explicitly added a type for rarely used platforms * PT 81767718
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@ -55,11 +55,10 @@ import org.broadinstitute.gatk.engine.recalibration.ReadCovariates;
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import org.broadinstitute.gatk.engine.recalibration.RecalibrationArgumentCollection;
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import org.broadinstitute.gatk.utils.BaseUtils;
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import org.broadinstitute.gatk.utils.NGSPlatform;
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import org.broadinstitute.gatk.utils.SequencerFlowClass;
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import org.broadinstitute.gatk.utils.exceptions.UserException;
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import org.broadinstitute.gatk.utils.sam.GATKSAMRecord;
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import java.util.EnumSet;
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/*
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* Copyright (c) 2009 The Broad Institute
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*
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@ -104,9 +103,6 @@ public class CycleCovariate implements StandardCovariate {
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public static final int CUSHION_FOR_INDELS = 4;
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private String default_platform = null;
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private static final EnumSet<NGSPlatform> DISCRETE_CYCLE_PLATFORMS = EnumSet.of(NGSPlatform.ILLUMINA, NGSPlatform.SOLID, NGSPlatform.PACBIO, NGSPlatform.COMPLETE_GENOMICS);
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private static final EnumSet<NGSPlatform> FLOW_CYCLE_PLATFORMS = EnumSet.of(NGSPlatform.LS454, NGSPlatform.ION_TORRENT);
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// Initialize any member variables using the command-line arguments passed to the walkers
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@Override
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public void initialize(final RecalibrationArgumentCollection RAC) {
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@ -126,7 +122,7 @@ public class CycleCovariate implements StandardCovariate {
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final NGSPlatform ngsPlatform = default_platform == null ? read.getNGSPlatform() : NGSPlatform.fromReadGroupPL(default_platform);
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// Discrete cycle platforms
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if (DISCRETE_CYCLE_PLATFORMS.contains(ngsPlatform)) {
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if (ngsPlatform.getSequencerType() == SequencerFlowClass.DISCRETE) {
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final int readOrderFactor = read.getReadPairedFlag() && read.getSecondOfPairFlag() ? -1 : 1;
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final int increment;
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int cycle;
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@ -149,7 +145,7 @@ public class CycleCovariate implements StandardCovariate {
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}
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// Flow cycle platforms
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else if (FLOW_CYCLE_PLATFORMS.contains(ngsPlatform)) {
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else if (ngsPlatform.getSequencerType() == SequencerFlowClass.FLOW) {
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final byte[] bases = read.getReadBases();
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@ -28,6 +28,7 @@ package org.broadinstitute.gatk.utils;
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import org.broadinstitute.gatk.utils.sam.GATKSAMReadGroupRecord;
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import org.broadinstitute.gatk.utils.sam.GATKSAMRecord;
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import java.util.Arrays;
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import java.util.LinkedList;
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import java.util.List;
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@ -42,38 +43,48 @@ import java.util.List;
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public enum NGSPlatform {
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// note the order of elements here determines the order of matching operations, and therefore the
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// efficiency of getting a NGSPlatform from a string.
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ILLUMINA("ILLUMINA", "SLX", "SOLEXA"),
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SOLID("SOLID"),
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LS454("454"),
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COMPLETE_GENOMICS("COMPLETE"),
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PACBIO("PACBIO"),
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ION_TORRENT("IONTORRENT"),
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CAPILLARY("CAPILLARY"),
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HELICOS("HELICOS"),
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UNKNOWN("UNKNOWN");
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ILLUMINA(SequencerFlowClass.DISCRETE, "ILLUMINA", "SLX", "SOLEXA"),
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SOLID(SequencerFlowClass.DISCRETE, "SOLID"),
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LS454(SequencerFlowClass.FLOW, "454", "LS454"),
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COMPLETE_GENOMICS(SequencerFlowClass.DISCRETE, "COMPLETE"),
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PACBIO(SequencerFlowClass.DISCRETE, "PACBIO"),
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ION_TORRENT(SequencerFlowClass.FLOW, "IONTORRENT"),
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CAPILLARY(SequencerFlowClass.OTHER, "CAPILLARY"),
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HELICOS(SequencerFlowClass.OTHER, "HELICOS"),
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UNKNOWN(SequencerFlowClass.OTHER, "UNKNOWN");
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/**
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* Array of the prefix names in a BAM file for each of the platforms.
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*/
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protected final String[] BAM_PL_NAMES;
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protected final SequencerFlowClass sequencerType;
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NGSPlatform(final String... BAM_PL_NAMES) {
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NGSPlatform(final SequencerFlowClass type, final String... BAM_PL_NAMES) {
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if ( BAM_PL_NAMES.length == 0 ) throw new IllegalStateException("Platforms must have at least one name");
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for ( int i = 0; i < BAM_PL_NAMES.length; i++ )
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BAM_PL_NAMES[i] = BAM_PL_NAMES[i].toUpperCase();
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this.BAM_PL_NAMES = BAM_PL_NAMES;
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this.sequencerType = type;
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}
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/**
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* Returns a representative PL string for this platform
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* @return
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* @return a representative PL string
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*/
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public final String getDefaultPlatform() {
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return BAM_PL_NAMES[0];
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}
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/**
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* The broad "type" of sequencer this platform represents (discrete or flow)
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* @return a SequencerFlowClass
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*/
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public final SequencerFlowClass getSequencerType() {
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return sequencerType;
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}
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/**
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* Convenience get -- get the NGSPlatform from a GATKSAMRecord.
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*
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@ -126,10 +137,9 @@ public enum NGSPlatform {
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* @return the list of platform names
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*/
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public static String knownPlatformsString() {
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final List<String> names = new LinkedList<String>();
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final List<String> names = new LinkedList<>();
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for ( final NGSPlatform pl : values() ) {
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for ( final String name : pl.BAM_PL_NAMES )
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names.add(name);
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names.addAll(Arrays.asList(pl.BAM_PL_NAMES));
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}
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return Utils.join(",", names);
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}
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@ -0,0 +1,38 @@
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/*
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* Copyright (c) 2012 The Broad Institute
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*
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* Permission is hereby granted, free of charge, to any person
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* obtaining a copy of this software and associated documentation
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* files (the "Software"), to deal in the Software without
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* restriction, including without limitation the rights to use,
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* copy, modify, merge, publish, distribute, sublicense, and/or sell
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* copies of the Software, and to permit persons to whom the
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* Software is furnished to do so, subject to the following
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* conditions:
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*
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* The above copyright notice and this permission notice shall be
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* included in all copies or substantial portions of the Software.
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*
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* THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND,
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* EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES
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* OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND
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* NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT
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* HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY,
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* WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING
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* FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR
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* THE USE OR OTHER DEALINGS IN THE SOFTWARE.
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*/
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package org.broadinstitute.gatk.utils;
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/**
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* In broad terms, each sequencing platform can be classified by whether it flows nucleotides in some order
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* such that homopolymers get sequenced in a single event (ie 454 or Ion) or it reads each position in the
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* sequence one at a time, regardless of base composition (Illumina or Solid). This information is primarily
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* useful in the BQSR process
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*/
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public enum SequencerFlowClass {
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DISCRETE,
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FLOW,
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OTHER //Catch-all for unknown platforms, as well as relics that GATK doesn't handle well (Capillary, Helicos)
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}
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