quick walker to find out where the reads mapped to huref were mapped in the consensus reference.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@5292 348d0f76-0448-11de-a6fe-93d51630548a
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/*
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* Copyright (c) 2010 The Broad Institute
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*
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* Permission is hereby granted, free of charge, to any person
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* obtaining a copy of this software and associated documentation
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* files (the "Software"), to deal in the Software without
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* restriction, including without limitation the rights to use,
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* copy, modify, merge, publish, distribute, sublicense, and/or sell
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* copies of the Software, and to permit persons to whom the
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* Software is furnished to do so, subject to the following
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* conditions:
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*
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* The above copyright notice and this permission notice shall be
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* included in all copies or substantial portions of the Software.
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*
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* THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND,
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* EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES
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* OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND
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* NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT
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* HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY,
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* WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING
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* FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR
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* THE USE OR OTHER DEALINGS IN THE SOFTWARE.
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*/
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package org.broadinstitute.sting.gatk.walkers;
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import net.sf.samtools.SAMFileWriter;
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import net.sf.samtools.SAMReadGroupRecord;
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import net.sf.samtools.SAMRecord;
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import org.broadinstitute.sting.commandline.Argument;
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import org.broadinstitute.sting.commandline.Output;
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import org.broadinstitute.sting.gatk.contexts.ReferenceContext;
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import org.broadinstitute.sting.gatk.refdata.ReadMetaDataTracker;
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import org.broadinstitute.sting.utils.baq.BAQ;
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import org.broadinstitute.sting.utils.exceptions.UserException;
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import org.broadinstitute.sting.utils.text.XReadLines;
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import java.io.File;
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import java.io.FileNotFoundException;
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import java.util.HashSet;
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import java.util.Set;
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/**
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* Renders, in SAM/BAM format, all reads from the input data set in the order in which they appear
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* in the input file. It can dynamically merge the contents of multiple input BAM files, resulting
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* in merged output sorted in coordinate order. Can also optionally filter reads based on the --read-filter
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* command line argument.
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*/
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@BAQMode(QualityMode = BAQ.QualityMode.ADD_TAG, ApplicationTime = BAQ.ApplicationTime.ON_OUTPUT)
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@Requires({DataSource.READS, DataSource.REFERENCE})
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public class FindReadsWithNamesWalker extends ReadWalker<SAMRecord, SAMFileWriter> {
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/** an optional argument to dump the reads out to a BAM file */
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@Output(doc="Write output to this BAM filename instead of STDOUT")
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SAMFileWriter out;
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@Argument(fullName = "readNamesToKeep", shortName = "rn", doc="names to keep", required = true)
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File readNamesFile = null;
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Set<String> namesToKeep;
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/**
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* The initialize function.
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*/
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public void initialize() {
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try {
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namesToKeep = new HashSet<String>(new XReadLines(readNamesFile).readLines());
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} catch (FileNotFoundException e) {
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throw new UserException.CouldNotReadInputFile(readNamesFile, e);
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}
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}
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/**
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* The reads filter function.
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* @param ref the reference bases that correspond to our read, if a reference was provided
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* @param read the read itself, as a SAMRecord
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* @return true if the read passes the filter, false if it doesn't
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*/
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public boolean filter(ReferenceContext ref, SAMRecord read) {
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return namesToKeep.contains(read.getReadName());
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}
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/**
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* The reads map function.
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* @param ref the reference bases that correspond to our read, if a reference was provided
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* @param read the read itself, as a SAMRecord
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* @return the read itself
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*/
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public SAMRecord map( ReferenceContext ref, SAMRecord read, ReadMetaDataTracker metaDataTracker ) {
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return read;
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}
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/**
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* reduceInit is called once before any calls to the map function. We use it here to setup the output
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* bam file, if it was specified on the command line
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* @return SAMFileWriter, set to the BAM output file if the command line option was set, null otherwise
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*/
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public SAMFileWriter reduceInit() {
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return out;
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}
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/**
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* given a read and a output location, reduce by emitting the read
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* @param read the read itself
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* @param output the output source
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* @return the SAMFileWriter, so that the next reduce can emit to the same source
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*/
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public SAMFileWriter reduce( SAMRecord read, SAMFileWriter output ) {
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output.addAlignment(read);
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return output;
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}
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}
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