Very early draft of script integrating the covariant counting / logistic regression. Deleted some unused code and spurious debug info.

git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@902 348d0f76-0448-11de-a6fe-93d51630548a

java/src/org/broadinstitute/sting/playground/gatk/walkers/LogisticRecalibrationWalker.java

@@ -91,7 +91,6 @@ public class LogisticRecalibrationWalker extends ReadWalker<SAMRecord, SAMFileWr
 
 
     public SAMRecord map(char[] ref, SAMRecord read) {
-        System.out.println(String.format("Reading: %s (%s:%d-%d)",read.getReadName(),read.getReferenceName(),read.getAlignmentStart(),read.getAlignmentEnd()));              
         SAMRecord recalRead = read;
         byte[] bases = read.getReadBases();
         byte[] quals = read.getBaseQualities();

python/LogisticRegressionByReadGroup.py

@@ -56,13 +56,6 @@ def process_file(source_file,read_group,dinuc,target_file):
     target_file.write('\t'.join([read_group,dinuc]+parameters)+'\n')
     os.remove('.'.join((base,dinuc,'csv')))
     os.remove('.'.join((base,dinuc,'parameters')))
-
-class LogisticRegressionRunner:
-    def __init__(self,source_filename,read_group,dinuc):
-        __base = source_file.name[:source_file.name.rfind('.csv')]
-        __read_group = read_group
-        __dinuc = dinuc
-        
     
 if len(sys.argv) < 3:
     exit("Usage: logistic_regression <covariate count input csv> <parameters csv>",1)

python/RecalQual.py

@@ -0,0 +1,68 @@
+#!/usr/bin/env python
+
+# Executable files.  Please update these to match the installed locations of your tools.
+samtools_exe='/seq/dirseq/samtools/current/samtools'
+java_exe='/broad/tools/Linux/x86_64/pkgs/jdk_1.6.0_12/bin/java'
+R_exe='/util/bin/R'
+GATK_jar='/humgen/gsa-scr1/hanna/src/StingWorking/dist/GenomeAnalysisTK.jar'
+
+# Location of the resource files distributed with the recalibration tool.
+resources='resources/'
+
+# Where does the reference live?
+reference_base = resources + 'Homo_sapiens_assembly18'
+reference      = reference_base + '.fasta'
+reference_dict = reference_base + '.dict'
+reference_fai  = reference_base + '.fasta.fai'
+
+# Where does DBSNP live?
+dbsnp = resources + 'dbsnp.rod.out'
+
+import sys,os
+
+def exit(msg,errorcode):
+    print msg
+    sys.exit(errorcode)
+
+def check_input_file_available(filename,description):
+    if not os.access(filename,os.R_OK):
+        exit('Unable to access %s %s' % (description,filename),1)
+
+if len(sys.argv) < 3:
+    exit('Usage: python RecalQual.py <input bam file> <calibrated output bam file>',1)
+
+# check that the input bam file exists, and that the bam is indexed.
+bam = sys.argv[1]
+bam_index = bam + '.bai'
+
+check_input_file_available(bam,'reads file')
+check_input_file_available(bam_index,'reads index file')
+
+# parse the user's calibration output file requirements
+calibrated_bam = sys.argv[2]
+calibrated_bam_index = calibrated_bam + '.bai'
+
+# check that the fasta and supporting files are available
+check_input_file_available(reference,'reference file')
+check_input_file_available(reference_dict,'reference dictionary')
+check_input_file_available(reference_fai,'reference index file')
+
+# check that the dbsnp is available
+check_input_file_available(dbsnp,'dbsnp file')
+
+# make an output directory for temporary files
+if not os.path.isdir('output'):
+    os.mkdir('output')
+
+# assemble the required program arguments
+gatk_base_cmdline = ' '.join((java_exe,'-ea','-jar',GATK_jar,'-R',reference,'--DBSNP',dbsnp,'-l INFO','-L chrM'))
+
+generate_covariates         = ' '.join((gatk_base_cmdline,'-T CountCovariates','-I',bam,'-mqs 40','--OUTPUT_FILEROOT output/output','--CREATE_TRAINING_DATA','--MIN_MAPPING_QUALITY 1'))
+compute_logistic_regression = ' '.join(('python','LogisticRegressionByReadGroup.py','output/output.covariate_counts.csv','output/linear_regression_results.out'))
+apply_logistic_regression   = ' '.join((gatk_base_cmdline,'-T LogisticRecalibration','-I',bam,'-logisticParams output/linear_regression_results.out','-outputBAM',calibrated_bam))
+index_calibrated_bamfile    = ' '.join((samtools_exe,'index',calibrated_bam))
+
+os.system(generate_covariates)
+os.system(compute_logistic_regression)
+os.system(apply_logistic_regression)
+os.system(index_calibrated_bamfile)