Putting the annotation back in, both to the filters and to UG
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4709 348d0f76-0448-11de-a6fe-93d51630548a
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@ -206,6 +206,8 @@ class fullCallingPipeline extends QScript {
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snps.jobOutputFile = new File(".queue/logs/SNPCalling/UnifiedGenotyper.out")
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snps.jobOutputFile = new File(".queue/logs/SNPCalling/UnifiedGenotyper.out")
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snps.analysisName = base+"_SNP_calls"
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snps.analysisName = base+"_SNP_calls"
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snps.input_file = bamFiles
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snps.input_file = bamFiles
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snps.annotation ++= List("AlleleBalance")
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snps.input_file = bamFiles
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snps.group :+= "Standard"
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snps.group :+= "Standard"
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snps.out = new File("SnpCalls", base+".vcf")
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snps.out = new File("SnpCalls", base+".vcf")
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snps.standard_min_confidence_threshold_for_emitting = Some(10)
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snps.standard_min_confidence_threshold_for_emitting = Some(10)
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@ -309,8 +311,8 @@ class fullCallingPipeline extends QScript {
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handFilter.jobOutputFile = new File(".queue/logs/SNPCalling/HandFilter.out")
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handFilter.jobOutputFile = new File(".queue/logs/SNPCalling/HandFilter.out")
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handFilter.variantVCF = masker.out
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handFilter.variantVCF = masker.out
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handFilter.rodBind :+= RodBind("mask", "VCF", mergeIndels.out)
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handFilter.rodBind :+= RodBind("mask", "VCF", mergeIndels.out)
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handFilter.filterName ++= List("StrandBias","QualByDepth","HomopolymerRun")
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handFilter.filterName ++= List("StrandBias","AlleleBalance","QualByDepth","HomopolymerRun")
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handFilter.filterExpression ++= List("\"SB>=0.10\"","\"QD<5.0\"","\"HRun>=4\"")
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handFilter.filterExpression ++= List("\"SB>=0.10\"","\"AB>=0.75\"","\"QD<5.0\"","\"HRun>=4\"")
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handFilter.out = swapExt("SnpCalls",annotated.out,".vcf",".handfiltered.vcf")
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handFilter.out = swapExt("SnpCalls",annotated.out,".vcf",".handfiltered.vcf")
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handFilter.analysisName = base+"_HandFilter"
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handFilter.analysisName = base+"_HandFilter"
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