Changed the OUTPUT_BAM_FILE argument from String to SAMFileWriter and removed the call to close().
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@2551 348d0f76-0448-11de-a6fe-93d51630548a
This commit is contained in:
parent
7893aaefe9
commit
80af0f2f54
|
|
@ -71,7 +71,7 @@ public class TableRecalibrationWalker extends ReadWalker<SAMRecord, SAMFileWrite
|
|||
// Command Line Arguments
|
||||
/////////////////////////////
|
||||
@Argument(fullName="output_bam", shortName="outputBam", doc="output BAM file", required=true)
|
||||
private String OUTPUT_BAM_FILE = null;
|
||||
private SAMFileWriter OUTPUT_BAM = null;
|
||||
@Argument(fullName="preserve_qscores_less_than", shortName="pQ",
|
||||
doc="Bases with quality scores less than this threshold won't be recalibrated, default=5. In general it's unsafe to change qualities scores below < 5, since base callers use these values to indicate random or bad bases", required=false)
|
||||
private int PRESERVE_QSCORES_LESS_THAN = 5;
|
||||
|
|
@ -95,7 +95,6 @@ public class TableRecalibrationWalker extends ReadWalker<SAMRecord, SAMFileWrite
|
|||
private static final Pattern OLD_RECALIBRATOR_HEADER = Pattern.compile("^rg,.*");
|
||||
private static final Pattern COVARIATE_PATTERN = Pattern.compile("^ReadGroup,QualityScore,.*");
|
||||
private static final String versionString = "v2.2.6"; // Major version, minor version, and build number
|
||||
private SAMFileWriter OUTPUT_BAM = null;// The File Writer that will write out the recalibrated bam
|
||||
private Random coinFlip; // Random number generator is used to remove reference bias in solid bams
|
||||
private static final long RANDOM_SEED = 1032861495;
|
||||
|
||||
|
|
@ -246,12 +245,6 @@ public class TableRecalibrationWalker extends ReadWalker<SAMRecord, SAMFileWrite
|
|||
programRecord.setCommandLine( commandLineString );
|
||||
header.addProgramRecord( programRecord );
|
||||
}
|
||||
|
||||
// Create the SAMFileWriter that we will be using to output the reads
|
||||
if( OUTPUT_BAM_FILE != null ) {
|
||||
final SAMFileWriterFactory factory = new SAMFileWriterFactory();
|
||||
OUTPUT_BAM = factory.makeBAMWriter( header, true, new File(OUTPUT_BAM_FILE), 5 ); // BUGBUG: Bam compression hardcoded to 5
|
||||
}
|
||||
}
|
||||
|
||||
/**
|
||||
|
|
@ -451,12 +444,7 @@ public class TableRecalibrationWalker extends ReadWalker<SAMRecord, SAMFileWrite
|
|||
}
|
||||
|
||||
/**
|
||||
* Close the output bam file
|
||||
* @param output The SAMFileWriter that outputs the bam file
|
||||
*/
|
||||
public void onTraversalDone(SAMFileWriter output) {
|
||||
if ( output != null ) {
|
||||
output.close();
|
||||
}
|
||||
}
|
||||
public void onTraversalDone(SAMFileWriter output) { }
|
||||
}
|
||||
|
|
|
|||
Loading…
Reference in New Issue