Several improvements to AssessNA12878 and KB
-- Bugfix for BAMs containing reads without real (M,I,D,N) operators. Simply needed to set validation stringency to SILENT in the read. Added a BadCigar filter to the SAMRecord stream anyway -- Add capture all sites mode to AssessNA12878: will write all sites to the badSites VCF, regardless of whether they are bad. It's useful if you essentially want to annotate a VCF with KB information for later analysis, such as computing ROC curves -- Add ignore filters mode to AssessNA12878: will as expected treat all sites in the input VCF calls as PASS, even if the site has a FILTER field setting -- Add minPNonRef argument to AssessNA12878: this will consider a site not called even if the NA12878 genotype is not 0/0 if the PLs are present and the PL for 0/0 isn't greater than this value. It allows us to easily differentiate low confidence non-ref sites obtained via multi-sample calling from highly confident non-ref calls that might be real TP or FPs
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@ -29,7 +29,8 @@ import com.google.java.contract.Ensures;
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import com.google.java.contract.Requires;
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import net.sf.samtools.CigarOperator;
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import net.sf.samtools.SAMFileReader;
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import net.sf.samtools.SAMRecordIterator;
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import net.sf.samtools.SAMRecord;
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import net.sf.samtools.util.CloseableIterator;
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import org.apache.log4j.Logger;
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import org.broadinstitute.sting.gatk.ReadProperties;
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import org.broadinstitute.sting.gatk.contexts.AlignmentContext;
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@ -154,7 +155,7 @@ public final class LocusIteratorByState extends LocusIterator {
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* @param reader a non-null reader
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* @param it an iterator from reader that has the reads we want to use to create ReadBackPileups
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*/
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public LocusIteratorByState(final SAMFileReader reader, final SAMRecordIterator it) {
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public LocusIteratorByState(final SAMFileReader reader, final CloseableIterator<SAMRecord> it) {
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this(new GATKSAMIterator(it),
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new LIBSDownsamplingInfo(false, 0),
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true,
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