Fix for Solid reads that have '.' in their color space field. The recalibrator will just set them to be illumina reads and won't apply color space correction.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@2661 348d0f76-0448-11de-a6fe-93d51630548a
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@ -259,12 +259,16 @@ public class RecalDataManager {
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if( attr != null ) {
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if( attr != null ) {
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char[] colorSpace;
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char[] colorSpace;
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if( attr instanceof String ) {
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if( attr instanceof String ) {
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if( ((String)attr).contains(".") ) { // empty color space, abort
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read.getReadGroup().setPlatform("illumina");
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return;
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}
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colorSpace = ((String)attr).toCharArray();
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colorSpace = ((String)attr).toCharArray();
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} else {
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} else {
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throw new StingException(String.format("Value encoded by %s in %s isn't a string!", RecalDataManager.COLOR_SPACE_ATTRIBUTE_TAG, read.getReadName()));
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throw new StingException(String.format("Value encoded by %s in %s isn't a string!", RecalDataManager.COLOR_SPACE_ATTRIBUTE_TAG, read.getReadName()));
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}
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}
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// Loop over the read and calculate first the infered bases from the color and then check if it is consistent with the read
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// Loop over the read and calculate first the inferred bases from the color and then check if it is consistent with the read
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byte[] readBases = read.getReadBases();
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byte[] readBases = read.getReadBases();
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if( read.getReadNegativeStrandFlag() ) {
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if( read.getReadNegativeStrandFlag() ) {
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readBases = BaseUtils.simpleReverseComplement( read.getReadBases() );
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readBases = BaseUtils.simpleReverseComplement( read.getReadBases() );
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@ -304,6 +308,10 @@ public class RecalDataManager {
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if( attr != null ) {
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if( attr != null ) {
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char[] colorSpace;
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char[] colorSpace;
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if( attr instanceof String ) {
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if( attr instanceof String ) {
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if( ((String)attr).contains(".") ) { // empty color space, abort
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read.getReadGroup().setPlatform("illumina");
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return originalQualScores;
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}
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colorSpace = ((String)attr).toCharArray();
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colorSpace = ((String)attr).toCharArray();
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} else {
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} else {
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throw new StingException(String.format("Value encoded by %s in %s isn't a string!", RecalDataManager.COLOR_SPACE_ATTRIBUTE_TAG, read.getReadName()));
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throw new StingException(String.format("Value encoded by %s in %s isn't a string!", RecalDataManager.COLOR_SPACE_ATTRIBUTE_TAG, read.getReadName()));
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