Utility method added: getQualsInCycleOrder(read) - examines the read and returns its quals in the order the machine read them (i.e. always from cycle 1 to cycle N). Simply inverts quals if the read happens to be rc-aligned :)
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@3183 348d0f76-0448-11de-a6fe-93d51630548a
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@ -339,4 +339,17 @@ public class AlignmentUtils {
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&& r.getAlignmentStart() != SAMRecord.NO_ALIGNMENT_START ) return false ;
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return true;
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}
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/** Returns the array of base qualitites in the order the bases were read on the machine (i.e. always starting from
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* cycle 1). In other words, if the read is unmapped or aligned in the forward direction, the read's own base
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* qualities are returned as stored in the SAM record; if the read is aligned in the reverse direction, the array
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* of read's base qualitites is inverted (in this case new array is allocated and returned).
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* @param read
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* @return
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*/
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public static byte [] getQualsInCycleOrder(SAMRecord read) {
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if ( isReadUnmapped(read) || ! read.getReadNegativeStrandFlag() ) return read.getBaseQualities();
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return BaseUtils.reverse(read.getBaseQualities());
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}
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}
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