Reverting the DPP to the original version, going to create a new simplified version for CMI in private.
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4324bd72fd
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@ -96,7 +96,6 @@ class DataProcessingPipeline extends QScript {
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var cleanModelEnum: ConsensusDeterminationModel = ConsensusDeterminationModel.USE_READS
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val bwaParameters: String = " -q 5 -l 32 -k 2 -t 4 -o 1 "
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@ -166,15 +165,12 @@ class DataProcessingPipeline extends QScript {
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var realignedBams: Seq[File] = Seq()
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var index = 1
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for (bam <- bams) {
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val extension = bam.toString.substring(bam.toString.length - 4)
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val saiFile1 = swapExt(bam, extension, "." + index + ".1.sai")
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val saiFile2 = swapExt(bam, extension, "." + index + ".2.sai")
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val realignedSamFile = swapExt(bam, extension, "." + index + ".realigned.sam")
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val realignedBamFile = swapExt(bam, extension, "." + index + ".realigned.bam")
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val rgRealignedBamFile = swapExt(bam, extension, "." + index + ".realigned.rg.bam")
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// first revert the BAM file to the original qualities
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val saiFile1 = swapExt(bam, ".bam", "." + index + ".1.sai")
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val saiFile2 = swapExt(bam, ".bam", "." + index + ".2.sai")
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val realignedSamFile = swapExt(bam, ".bam", "." + index + ".realigned.sam")
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val realignedBamFile = swapExt(bam, ".bam", "." + index + ".realigned.bam")
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val rgRealignedBamFile = swapExt(bam, ".bam", "." + index + ".realigned.rg.bam")
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if (useBWAse) {
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val revertedBAM = revertBAM(bam, true)
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@ -448,7 +444,7 @@ class DataProcessingPipeline extends QScript {
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case class bwa_aln_se (inBam: File, outSai: File) extends CommandLineFunction with ExternalCommonArgs {
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@Input(doc="bam file to be aligned") var bam = inBam
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@Output(doc="output sai file") var sai = outSai
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def commandLine = bwaPath + " aln -t " + bwaThreads + bwaParameters + reference + " -b " + bam + " > " + sai
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def commandLine = bwaPath + " aln -t " + bwaThreads + " -q 5 " + reference + " -b " + bam + " > " + sai
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this.analysisName = queueLogDir + outSai + ".bwa_aln_se"
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this.jobName = queueLogDir + outSai + ".bwa_aln_se"
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}
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@ -456,7 +452,7 @@ class DataProcessingPipeline extends QScript {
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case class bwa_aln_pe (inBam: File, outSai1: File, index: Int) extends CommandLineFunction with ExternalCommonArgs {
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@Input(doc="bam file to be aligned") var bam = inBam
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@Output(doc="output sai file for 1st mating pair") var sai = outSai1
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def commandLine = bwaPath + " aln -t " + bwaThreads + bwaParameters + reference + " -b" + index + " " + bam + " > " + sai
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def commandLine = bwaPath + " aln -t " + bwaThreads + " -q 5 " + reference + " -b" + index + " " + bam + " > " + sai
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this.analysisName = queueLogDir + outSai1 + ".bwa_aln_pe1"
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this.jobName = queueLogDir + outSai1 + ".bwa_aln_pe1"
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}
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